Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Forward (+)

10659172 .. 10664260

Gene Structure 5089 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1117700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3237 bp

ATGAAGCGTGATCACATTGCCAGAAGGAAAAAGAAACTCGATCCCATGGGGAAAGGAGGGCAGAACTACGGGAAGGGAGAGAGTCCGAATCCGATTCCGATTCCGAAAGCCGATCACTCTTTCCCGGCGTGGTCGAGAGACGCCAAGGAATGCGAGGACGCGTTCCGGGTGAGTCGCGACTCGGGCTTGTCCTCCGAGGAAGTCGACAACCGGCGGAAGGTGTACGGATGGAACGAGCTGGAGAAGCACGAGGACCAGTCGATGTGGAAGCTGTTGCTGGACCAGCTGAGCGACACGCTGGTGCGGATTCTGCTGGCGGCGGCGATCATCTCGTTTGTCCTGGCCTGGTTCGACGGCGAGGAGGAGAAGGAGATCACGGCGTTTGTGGAGCCGCTGGTGATTTTCCTGATCCTGATCGTGAATGCAATCGTCGGGATTTGGCAGGAGAGCAACGCGGAGAAGGCGCTGGAGGCGTTGAAGGAGATTCAGTCAGAGCAGGCGGCGGTAATTAGGGATGGACGGAAGGTGTCGAATCTGTCGGCGAAGGAGCTGGTTCCGGGGGATATTGTGGAGCTGAGAGTTGGGGATAAGGTGCCGGCGGATATGAGGGTTTTGAACTTGGTGAGCTCGACGTTTAGGGTTGAGCAGGGGTCTTTGACAGGAGAGAGTGAGGCAGTGAGCAAGAGCGTGAAGGCGGTTTCAGAGAATTCGGATATTCAAGGGAAGAAATGTATGGTTTTTGCAGGGACGACTGTGGTGAATGGGCATTGTGTTTGTTTGGTGACGCAGATTGGGATGAGGACTGAGATTGGGCAAGTGCATTCTCAGATTCAAGAAGCTTCGCAATGCGAGAACGATACCCCGCTGAAGAAGAAGCTGAATGAGTTTGGGGAGATTTTGACTATGATCATTGGAGTTATTTGTGCAATTGTTTGGCTCATCAATGTCAAGTACTTTCTCAGTTGGGAGATTGTTGATGGTTTGCCAACCAACTTTCAATTCTCGTTCGAGAAGTGTACTTATTACTTCAAGATTGCTGTGGCATTGGCTGTGGCAGCCATTCCTGAAGGCTTGCCTGCCGTGATCACGACTTGTTTGGCGCTTGGAACACGAAGGATGGCTCAGAAGAATGCACTTGTTAGGAAGTTGCCGAGTGTTGAGACTCTCGGGTGCACTACTGTGATTTGTTCTGATAAAACTGGTACTCTGACTACCAATCAGATGGCTGTGGCAAAGCTTGTGGCTATTGGTTCGAGGCCTGGTACTCTTCGAGCCTTCGATGTGGAAGGAACCACCTACAACCCTTTCGATGGGAAAATACAGGACTGGCCGCCAGCTGGTGATATGGATACCAATGTTCAAACGATAGCCAAAATCGCTGCTGTGTGTAATGATGCTGGTGTTGAGTTATCTGGGAGTCATTATGTTGCCAACGGGATGCCCACCGAGGCAGCTTTGAAGGTTCTGGTTGAGAAGATGGGAGTACCTGAAGTATTAAATACTTGTTTCTCCTCAGTTGGAGATCTACTACGTTGTTCTCAACTATGGAATGACATTGACCGGCGAATTGCAACACTGGAGTTTGACCGTGATCGCAAATCCATGAGTGTTATTGTGAATTCGGGCTCGGGAAAAAACTTACTTTTTGTTAAGGGTGCAGTTGAAAATGTATTGGAACGGAGCTCGTCCATTCAGTTGGTTGACGGCTCCATTGTAGAATTGGATCAAAAAATAAGGGATCTTATCTTGGGCACCCTTAATGAAATGTCAAGTAATGCATTACGTTGTTTGGGGTTCGCATACAAGGACGACCTCCCAGAGTTTGCAATGTACAATGGTGATGAAGATCATCCAGCTCATCAGCTTTTACTCAATCCTTCCAATTATTCTTCAATTGAGAATAAACTTGTTTTTGTTGGCATGGTCGGGCTAAGGGATCCTCCGCGAAAAGAGGTCCGTCAAGCCATTGAGGACTGCAAAGCTGCCGGAATCCGTGTTATGGTTATTACAGGAGACAACAAGAATACGGCTGAAGCTATTTGCCGTGAAATAGGTGTATTTAAACCTTCAGAAGATATTAGTTTGAAAAGCTTAACAGGAAAAGAATTCATCGATCACCATGACCAGAAAAGTTATTTAAGACAAAGTGGAGGACTGTTGTTCTCTAGGGCTGAGCCAAGGCATAAACAAGAGATAGTGAGGCTGCTCAAGGAAGACGGTGAAGTGGTTGCAATGACTGGTGATGGAGTGAATGATGCACCTGCCTTGAAGTTGGCTGATATTGGGATTGCAATGGGCATCGCTGGGACAGAGGTTGCCAAGGAAGCCTCTGATATGGTATTAGCAGATGACAATTTTAGCACTATAGTTGCTGCTGTCGGAGAAGGCAGGTCTATTTACAACAACATGAAGGCTTTTATCAGGTACATGATCTCCTCAAACATTGGCGAAGTTGCATCCATTTTTCTGACAGCCGCCTTGGGTATTCCAGAAGGGATGATCCCAGTTCAGCTTCTCTGGGTCAATCTGGTCACTGATGGACCCCCAGCAACAGCTTTGGGATTCAATCCACCAGACAAAGACATAATGGAGAAGCCCCCTAGAAGAAGCAACGAGTCATTGATCACTCCTTGGATCTTATTCCGTTATCTGGTAATCGGGCTCTACGTCGGGACAGCAACAGTTGGGGTGTTCATTATTTGGTACACTCATTCCTCATTTTTGGGCATCGACCTTAGCCAAGACGGCCATAGTCTAGTGACCTACTCTCAACTTGCAAACTGGGGCAAGTGCCCTTCCTGGGAAGGATTCTCACCATCTAACTTCACAGCCGGATCCCAGGTGTTCACTTTCGACACCAATCCATGCGAATATTTCCACACTGGAAAAATCAAAGCCTCAACTCTCTCCCTCTCCGTCTTGGTTGCCATCGAGATGTTCAACTCCCTCAATGCACTATCTGAGGACGGAAGCCTCTTGACAATGCCTCCCTGGGTCAATCCGTGGCTCCTTCTAGCCATGTCCGTGTCATTCGGCTTGCACTTTCTGATCCTCTACATCCCGTTCCTTGCGAATGTGTTCAGCATTGTCCCTCTCAGCCTGAACGAATGGCTCTTGGTTCTGGCTGCCGCGCTTCCGGTGATCTTGATCGATGAGATTCTCAAGTTTGTGGGAAGATGTACGAGTAAGTTGCCGGATTCTCCTACTATCGACAGATCTAAGCAAAAAGCAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1079

Amino Acids

118.2

Weight (kDa)

5.32

Isoelectric Point (pI)

36.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	44 - 111	7.3e-18	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	162 - 277	1.5e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	453 - 651	4e-40	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	639 - 760	4.9e-16	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	832 - 1057	5.9e-49	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2269
Acc36I	ACCTGC	2 cut(s)	2269, 2379
AccB1I	GGYRCC	2 cut(s)	592, 1751
AccI	GTMKAC	1 cut(s)	204
AccII	CGCG	5 cut(s)	161, 177, 455, 1945, 3131
AcoI	YGGCCR	2 cut(s)	1328, 2746
AcsI	RAATTY	3 cut(s)	706, 1618, 2105
AcuI	CTGAAG	5 cut(s)	887, 1086, 1509, 2052, 2052
AcyI	GRCGYC	1 cut(s)	141
AfiI	CCNNNNNNNGG	6 cut(s)	217, 1310, 1337, 1999, 2650, 2800
AflIII	ACRYGT	1 cut(s)	159
AjnI	CCWGG	6 cut(s)	339, 344, 1258, 2798, 2838, 2990
AjuI	GAANNNNNNNTTGG	2 cut(s)	983, 1015
AleI	CACNNNNGTG	2 cut(s)	299, 1178
Alw21I	GWGCWC	3 cut(s)	629, 1175, 1685
Alw26I	GTCTC	3 cut(s)	132, 1154, 2007
Alw44I	GTGCAC	1 cut(s)	1171
Ama87I	CYCGRG	3 cut(s)	181, 1166, 1627
AoxI	GGCC	4 cut(s)	342, 1256, 1328, 2746
ApaLI	GTGCAC	1 cut(s)	1171
ApeKI	GCWGC	7 cut(s)	1055, 1379, 1451, 1982, 2203, 2372, 3125
ApoI	RAATTY	3 cut(s)	706, 1618, 2105
Asp700I	GAANNNNTTC	1 cut(s)	3077
AspLEI	GCGC	3 cut(s)	466, 1102, 3133
AspS9I	GGNCC	4 cut(s)	253, 280, 1954, 2540
AsuC2I	CCSGG	3 cut(s)	125, 167, 558
AvaI	CYCGRG	3 cut(s)	181, 1166, 1627
AvaII	GGWCC	4 cut(s)	253, 280, 1954, 2540
BaeGI	GKGCMC	3 cut(s)	1175, 1754, 2795
BaeI	ACNNNNGTAYC	2 cut(s)	1341, 1374
BamHI	GGATCC	2 cut(s)	1936, 2834
BanI	GGYRCC	2 cut(s)	592, 1751
BanII	GRGCYC	4 cut(s)	629, 1628, 1685, 2664
BauI	CACGAG	1 cut(s)	248
BbsI	GAAGAC	1 cut(s)	2220
Bbv12I	GWGCWC	3 cut(s)	629, 1175, 1685
BbvI	GCAGC	7 cut(s)	1067, 1366, 1463, 1969, 2190, 2359, 3112
BceAI	ACGGC	7 cut(s)	370, 393, 1064, 1720, 2028, 2043, 2761
BcgI	CGANNNNNNTGC	2 cut(s)	3172, 3206
BciT130I	CCWGG	6 cut(s)	341, 346, 1260, 2800, 2840, 2992
BciVI	GTATCC	1 cut(s)	1342
BclI	TGATCA	4 cut(s)	10, 906, 1083, 2622
BcnI	CCSGG	3 cut(s)	125, 167, 558
BcoDI	GTCTC	3 cut(s)	132, 1154, 2007
BfaI	CTAG	4 cut(s)	2166, 2601, 2756, 3014
BfmI	CTRYAG	1 cut(s)	2364
BfoI	RGCGCY	2 cut(s)	467, 1103
BfuAI	ACCTGC	2 cut(s)	2269, 2379
BfuI	GTATCC	1 cut(s)	1342
BglI	GCCNNNNNGGC	1 cut(s)	2745
BglII	AGATCT	2 cut(s)	1522, 3215
BlpI	GCTNAGC	2 cut(s)	287, 2172
BmcAI	AGTACT	1 cut(s)	953
Bme1390I	CCNGG	9 cut(s)	125, 167, 341, 346, 558, 1260, 2800, 2840, 2992
Bme18I	GGWCC	4 cut(s)	253, 280, 1954, 2540
BmeT110I	CYCGRG	3 cut(s)	181, 1166, 1627
BmgT120I	GGNCC	4 cut(s)	253, 280, 1954, 2540
BmrFI	CCNGG	9 cut(s)	125, 167, 341, 346, 558, 1260, 2800, 2840, 2992
BmrI	ACTGGG	2 cut(s)	2498, 2791
BmsI	GCATC	6 cut(s)	1384, 1428, 2245, 2307, 2465, 2736
BmuI	ACTGGG	2 cut(s)	2498, 2791
BpiI	GAAGAC	1 cut(s)	2220
BpmI	CTGGAG	3 cut(s)	260, 488, 1598
Bpu10I	CCTNAGC	2 cut(s)	1931, 2735
Bpu1102I	GCTNAGC	2 cut(s)	287, 2172
BpuEI	CTTGAG	2 cut(s)	2192, 3146
BpuMI	CCSGG	3 cut(s)	125, 167, 558
Bsa29I	ATCGAT	2 cut(s)	2112, 3150
BsaBI	GATNNNNATC	3 cut(s)	413, 1845, 3146
BsaHI	GRCGYC	1 cut(s)	141
BsaWI	WCCGGW	1 cut(s)	3136
BsaXI	ACNNNNNCTCC	6 cut(s)	473, 503, 2417, 2447, 2971, 3001
Bsc4I	CCNNNNNNNGG	6 cut(s)	217, 1310, 1337, 1999, 2650, 2800
Bse118I	RCCGGY	3 cut(s)	210, 595, 1560
Bse1I	ACTGG	8 cut(s)	256, 1204, 1331, 1581, 2242, 2504, 2786, 2887
Bse3DI	GCAATG	5 cut(s)	15, 851, 1833, 2238, 2298
Bse8I	GATNNNNATC	3 cut(s)	413, 1845, 3146
BseBI	CCWGG	6 cut(s)	341, 346, 1260, 2800, 2840, 2992
BseCI	ATCGAT	2 cut(s)	2112, 3150
BseGI	GGATG	9 cut(s)	233, 520, 801, 1122, 1443, 1849, 2456, 2502, 3057
BseJI	GATNNNNATC	3 cut(s)	413, 1845, 3146
BseLI	CCNNNNNNNGG	6 cut(s)	217, 1310, 1337, 1999, 2650, 2800
BseMI	GCAATG	5 cut(s)	15, 851, 1833, 2238, 2298
BseNI	ACTGG	8 cut(s)	256, 1204, 1331, 1581, 2242, 2504, 2786, 2887
BseRI	GAGGAG	4 cut(s)	374, 377, 1501, 2425
BseSI	GKGCMC	3 cut(s)	1175, 1754, 2795
BseXI	GCAGC	7 cut(s)	1067, 1366, 1463, 1969, 2190, 2359, 3112
BseYI	CCCAGC	2 cut(s)	2303, 2545
BsgI	GTGCAG	1 cut(s)	1677
Bsh1236I	CGCG	5 cut(s)	161, 177, 455, 1945, 3131
BshFI	GGCC	4 cut(s)	344, 1258, 1330, 2748
BshNI	GGYRCC	2 cut(s)	592, 1751
BshVI	ATCGAT	2 cut(s)	2112, 3150
BsiHKAI	GWGCWC	3 cut(s)	629, 1175, 1685
BsiHKCI	CYCGRG	3 cut(s)	181, 1166, 1627
BslFI	GGGAC	4 cut(s)	760, 2320, 2686, 3074
BslI	CCNNNNNNNGG	6 cut(s)	217, 1310, 1337, 1999, 2650, 2800
BsmAI	GTCTC	3 cut(s)	132, 1154, 2007
BsmBI	CGTCTC	1 cut(s)	132
BsmFI	GGGAC	4 cut(s)	760, 2320, 2686, 3074
BsmI	GAATGC	4 cut(s)	155, 427, 820, 1135
BsnI	GGCC	4 cut(s)	344, 1258, 1330, 2748
BsoBI	CYCGRG	3 cut(s)	181, 1166, 1627
Bsp1286I	GDGCHC	7 cut(s)	629, 1175, 1628, 1685, 1754, 2664, 2795
Bsp1407I	TGTACA	1 cut(s)	1830
Bsp1720I	GCTNAGC	2 cut(s)	287, 2172
Bsp19I	CCATGG	1 cut(s)	45
Bsp68I	TCGCGA	1 cut(s)	177
BspANI	GGCC	4 cut(s)	344, 1258, 1330, 2748
BspDI	ATCGAT	2 cut(s)	2112, 3150
BspFNI	CGCG	5 cut(s)	161, 177, 455, 1945, 3131
BspMI	ACCTGC	2 cut(s)	2269, 2379
BspT107I	GGYRCC	2 cut(s)	592, 1751
BsrDI	GCAATG	5 cut(s)	15, 851, 1833, 2238, 2298
BsrFI	RCCGGY	3 cut(s)	210, 595, 1560
BsrGI	TGTACA	1 cut(s)	1830
BsrI	ACTGG	8 cut(s)	256, 1204, 1331, 1581, 2242, 2504, 2786, 2887
BssAI	RCCGGY	3 cut(s)	210, 595, 1560
BssNI	GRCGYC	1 cut(s)	141
BssSI	CACGAG	1 cut(s)	248
BssT1I	CCWWGG	6 cut(s)	45, 144, 2177, 2319, 2478, 2630
Bst2BI	CACGAG	1 cut(s)	248
Bst2UI	CCWGG	6 cut(s)	341, 346, 1260, 2800, 2840, 2992
Bst4CI	ACNGT	6 cut(s)	754, 1180, 1589, 2157, 2219, 2683
Bst6I	CTCTTC	1 cut(s)	1272
BstACI	GRCGYC	1 cut(s)	141
BstAUI	TGTACA	1 cut(s)	1830
BstC8I	GCNNGC	7 cut(s)	315, 498, 597, 1073, 1077, 1335, 3038
BstDSI	CCRYGG	2 cut(s)	45, 3002
BstF5I	GGATG	9 cut(s)	233, 520, 801, 1122, 1443, 1849, 2456, 2502, 3057
BstFNI	CGCG	5 cut(s)	161, 177, 455, 1945, 3131
BstH2I	RGCGCY	2 cut(s)	467, 1103
BstHHI	GCGC	3 cut(s)	466, 1102, 3133
BstMAI	GTCTC	3 cut(s)	132, 1154, 2007
BstNI	CCWGG	6 cut(s)	341, 346, 1260, 2800, 2840, 2992
BstSCI	CCNGG	9 cut(s)	123, 165, 339, 344, 556, 1258, 2798, 2838, 2990
BstSFI	CTRYAG	1 cut(s)	2364
BstSLI	GKGCMC	3 cut(s)	1175, 1754, 2795
BstUI	CGCG	5 cut(s)	161, 177, 455, 1945, 3131
BstV1I	GCAGC	7 cut(s)	1067, 1366, 1463, 1969, 2190, 2359, 3112
BstV2I	GAAGAC	1 cut(s)	2220
BstX2I	RGATCY	6 cut(s)	1522, 1738, 1936, 2634, 2834, 3215
BstXI	CCANNNNNNTGG	2 cut(s)	1222, 1696
BstYI	RGATCY	6 cut(s)	1522, 1738, 1936, 2634, 2834, 3215
Bsu15I	ATCGAT	2 cut(s)	2112, 3150
BsuI	GTATCC	1 cut(s)	1342
BsuRI	GGCC	4 cut(s)	344, 1258, 1330, 2748
BsuTUI	ATCGAT	2 cut(s)	2112, 3150
BtgI	CCRYGG	2 cut(s)	45, 3002
BtgZI	GCGATG	1 cut(s)	2284
BtsCI	GGATG	9 cut(s)	233, 520, 801, 1122, 1443, 1849, 2456, 2502, 3057
BtsI	GCAGTG	1 cut(s)	681
BtsIMutI	CAGTG	4 cut(s)	681, 1574, 2532, 2880
BtuMI	TCGCGA	1 cut(s)	177
BveI	ACCTGC	2 cut(s)	2269, 2379
Cac8I	GCNNGC	7 cut(s)	315, 498, 597, 1073, 1077, 1335, 3038
CfoI	GCGC	3 cut(s)	466, 1102, 3133
Cfr10I	RCCGGY	3 cut(s)	210, 595, 1560
Cfr13I	GGNCC	4 cut(s)	253, 280, 1954, 2540
ClaI	ATCGAT	2 cut(s)	2112, 3150
CseI	GACGC	3 cut(s)	149, 167, 793
CspCI	CAANNNNNGTGG	2 cut(s)	1221, 1256
CviAII	CATG	8 cut(s)	46, 1603, 1921, 2120, 2407, 2428, 2865, 3019
DraI	TTTAAA	1 cut(s)	2062
EaeI	YGGCCR	2 cut(s)	1328, 2746
Eam1104I	CTCTTC	1 cut(s)	1272
EarI	CTCTTC	1 cut(s)	1272
EciI	GGCGGA	2 cut(s)	229, 614
Ecl136II	GAGCTC	2 cut(s)	627, 1683
Eco130I	CCWWGG	6 cut(s)	45, 144, 2177, 2319, 2478, 2630
Eco147I	AGGCCT	1 cut(s)	1258
Eco24I	GRGCYC	4 cut(s)	629, 1628, 1685, 2664
Eco47I	GGWCC	4 cut(s)	253, 280, 1954, 2540
Eco53kI	GAGCTC	2 cut(s)	627, 1683
Eco57I	CTGAAG	5 cut(s)	887, 1086, 1509, 2052, 2052
Eco88I	CYCGRG	3 cut(s)	181, 1166, 1627
EcoICRI	GAGCTC	2 cut(s)	627, 1683
EcoRI	GAATTC	3 cut(s)	706, 1618, 2105
EcoRII	CCWGG	6 cut(s)	339, 344, 1258, 2798, 2838, 2990
EcoT14I	CCWWGG	6 cut(s)	45, 144, 2177, 2319, 2478, 2630
EcoT22I	ATGCAT	1 cut(s)	1780
EcoT38I	GRGCYC	4 cut(s)	629, 1628, 1685, 2664
ErhI	CCWWGG	6 cut(s)	45, 144, 2177, 2319, 2478, 2630
Esp3I	CGTCTC	1 cut(s)	132
FaeI	CATG	8 cut(s)	49, 1606, 1924, 2123, 2410, 2431, 2868, 3022
FaqI	GGGAC	4 cut(s)	760, 2320, 2686, 3074
FatI	CATG	8 cut(s)	45, 1602, 1920, 2119, 2406, 2427, 2864, 3018
FauI	CCCGC	1 cut(s)	870
FbaI	TGATCA	4 cut(s)	10, 906, 1083, 2622
FblI	GTMKAC	1 cut(s)	204
FokI	GGATG	9 cut(s)	240, 527, 808, 1129, 1450, 1836, 2443, 2509, 3044
FriOI	GRGCYC	4 cut(s)	629, 1628, 1685, 2664
FspBI	CTAG	4 cut(s)	2166, 2601, 2756, 3014
GlaI	GCGC	3 cut(s)	465, 1101, 3132
GsaI	CCCAGC	2 cut(s)	2307, 2549
GsuI	CTGGAG	3 cut(s)	260, 488, 1598
HaeII	RGCGCY	2 cut(s)	467, 1103
HaeIII	GGCC	4 cut(s)	344, 1258, 1330, 2748
HgaI	GACGC	3 cut(s)	149, 167, 793
HhaI	GCGC	3 cut(s)	466, 1102, 3133
Hin1I	GRCGYC	1 cut(s)	141
Hin1II	CATG	8 cut(s)	49, 1606, 1924, 2123, 2410, 2431, 2868, 3022
Hin6I	GCGC	3 cut(s)	464, 1100, 3131
HinP1I	GCGC	3 cut(s)	464, 1100, 3131
HincII	GTYRAC	2 cut(s)	205, 1702
HindII	GTYRAC	2 cut(s)	205, 1702
HindIII	AAGCTT	3 cut(s)	837, 1235, 2089
Hpy166II	GTNNAC	7 cut(s)	205, 223, 1017, 1173, 1702, 2706, 2847
Hpy8I	GTNNAC	7 cut(s)	205, 223, 1017, 1173, 1702, 2706, 2847
Hpy99I	CGWCG	4 cut(s)	356, 434, 634, 2672
HpyCH4III	ACNGT	6 cut(s)	754, 1180, 1589, 2157, 2219, 2683
HpyCH4IV	ACGT	4 cut(s)	632, 1531, 1783, 2667
HpySE526I	ACGT	4 cut(s)	632, 1531, 1783, 2667
Hsp92I	GRCGYC	1 cut(s)	141
Hsp92II	CATG	8 cut(s)	49, 1606, 1924, 2123, 2410, 2431, 2868, 3022
HspAI	GCGC	3 cut(s)	464, 1100, 3131
KroI	GCCGGC	1 cut(s)	595
KroNI	GCCGGC	1 cut(s)	597
Ksp22I	TGATCA	4 cut(s)	10, 906, 1083, 2622
LmnI	GCTCC	6 cut(s)	388, 547, 571, 1680, 1712, 3012
Lsp1109I	GCAGC	7 cut(s)	1067, 1366, 1463, 1969, 2190, 2359, 3112
LweI	GCATC	6 cut(s)	1384, 1428, 2245, 2307, 2465, 2736
MaeI	CTAG	4 cut(s)	2166, 2601, 2756, 3014
MaeII	ACGT	4 cut(s)	632, 1531, 1783, 2667
MaeIII	GTNAC	3 cut(s)	781, 2530, 2758
MfeI	CAATTG	2 cut(s)	927, 1893
MflI	RGATCY	6 cut(s)	1522, 1738, 1936, 2634, 2834, 3215
MhlI	GDGCHC	7 cut(s)	629, 1175, 1628, 1685, 1754, 2664, 2795
MluI	ACGCGT	1 cut(s)	159
MlyI	GAGTC	6 cut(s)	91, 173, 181, 1156, 1426, 2624
MmeI	TCCRAC	2 cut(s)	1498, 2359
Mph1103I	ATGCAT	1 cut(s)	1780
MroNI	GCCGGC	1 cut(s)	595
MroXI	GAANNNNTTC	1 cut(s)	3077
MseI	TTAA	6 cut(s)	1496, 1650, 1758, 2061, 2093, 2138
MslI	CAYNNNNRTG	4 cut(s)	299, 1178, 2933, 3023
MspA1I	CMGCKG	4 cut(s)	286, 394, 865, 1337
MspR9I	CCNGG	9 cut(s)	125, 167, 341, 346, 558, 1260, 2800, 2840, 2992
MunI	CAATTG	2 cut(s)	927, 1893
Mva1269I	GAATGC	4 cut(s)	155, 427, 820, 1135
MvaI	CCWGG	6 cut(s)	341, 346, 1260, 2800, 2840, 2992
MvnI	CGCG	5 cut(s)	161, 177, 455, 1945, 3131
NaeI	GCCGGC	1 cut(s)	597
NciI	CCSGG	3 cut(s)	125, 167, 558
NcoI	CCATGG	1 cut(s)	45
NgoMIV	GCCGGC	1 cut(s)	595
NlaIII	CATG	8 cut(s)	49, 1606, 1924, 2123, 2410, 2431, 2868, 3022
NmeAIII	GCCGAG	1 cut(s)	1176
NmuCI	GTSAC	3 cut(s)	781, 2530, 2758
NruI	TCGCGA	1 cut(s)	177
NsiI	ATGCAT	1 cut(s)	1780
OliI	CACNNNNGTG	2 cut(s)	299, 1178
PaqCI	CACCTGC	1 cut(s)	2269
PasI	CCCWGGG	1 cut(s)	2991
PceI	AGGCCT	1 cut(s)	1258
PcsI	WCGNNNNNNNCGW	2 cut(s)	231, 2664
PctI	GAATGC	4 cut(s)	155, 427, 820, 1135
PdiI	GCCGGC	1 cut(s)	597
PdmI	GAANNNNTTC	1 cut(s)	3077
PleI	GAGTC	6 cut(s)	90, 173, 180, 1156, 1425, 2623
PpsI	GAGTC	6 cut(s)	90, 173, 180, 1156, 1425, 2623
Psp124BI	GAGCTC	2 cut(s)	629, 1685
Psp6I	CCWGG	6 cut(s)	339, 344, 1258, 2798, 2838, 2990
PspFI	CCCAGC	2 cut(s)	2303, 2545
PspGI	CCWGG	6 cut(s)	339, 344, 1258, 2798, 2838, 2990
PspPI	GGNCC	4 cut(s)	253, 280, 1954, 2540
PsuI	RGATCY	6 cut(s)	1522, 1738, 1936, 2634, 2834, 3215
PvuII	CAGCTG	2 cut(s)	286, 1337
RruI	TCGCGA	1 cut(s)	177
RseI	CAYNNNNRTG	4 cut(s)	299, 1178, 2933, 3023
SacI	GAGCTC	2 cut(s)	629, 1685
SalI	GTCGAC	1 cut(s)	203
SaqAI	TTAA	6 cut(s)	1496, 1650, 1758, 2061, 2093, 2138
Sau96I	GGNCC	4 cut(s)	253, 280, 1954, 2540
ScaI	AGTACT	1 cut(s)	953
SchI	GAGTC	6 cut(s)	91, 173, 181, 1156, 1426, 2624
ScrFI	CCNGG	9 cut(s)	125, 167, 341, 346, 558, 1260, 2800, 2840, 2992
SduI	GDGCHC	7 cut(s)	629, 1175, 1628, 1685, 1754, 2664, 2795
SfaNI	GCATC	6 cut(s)	1384, 1428, 2245, 2307, 2465, 2736
SfcI	CTRYAG	1 cut(s)	2364
SinI	GGWCC	4 cut(s)	253, 280, 1954, 2540
SmiMI	CAYNNNNRTG	4 cut(s)	299, 1178, 2933, 3023
SmlI	CTYRAG	2 cut(s)	2207, 3161
SmoI	CTYRAG	2 cut(s)	2207, 3161
SseBI	AGGCCT	1 cut(s)	1258
SspI	AATATT	1 cut(s)	2873
SspMI	CTAG	4 cut(s)	2166, 2601, 2756, 3014
SstI	GAGCTC	2 cut(s)	629, 1685
StuI	AGGCCT	1 cut(s)	1258
StyD4I	CCNGG	9 cut(s)	123, 165, 339, 344, 556, 1258, 2798, 2838, 2990
StyI	CCWWGG	6 cut(s)	45, 144, 2177, 2319, 2478, 2630
TaaI	ACNGT	6 cut(s)	754, 1180, 1589, 2157, 2219, 2683
TaiI	ACGT	4 cut(s)	635, 1534, 1786, 2670
TatI	WGTACW	3 cut(s)	951, 1016, 1830
TauI	GCSGC	7 cut(s)	320, 323, 394, 503, 1333, 2477, 3131
Tru1I	TTAA	6 cut(s)	1496, 1650, 1758, 2061, 2093, 2138
Tru9I	TTAA	6 cut(s)	1496, 1650, 1758, 2061, 2093, 2138
TscAI	CASTG	4 cut(s)	681, 1581, 2539, 2887
TseFI	GTSAC	3 cut(s)	781, 2530, 2758
TseI	GCWGC	7 cut(s)	1055, 1379, 1451, 1982, 2203, 2372, 3125
Tsp45I	GTSAC	3 cut(s)	781, 2530, 2758
TspDTI	ATGAA	6 cut(s)	17, 1776, 1857, 2098, 2423, 2683
TspRI	CASTG	4 cut(s)	681, 1581, 2539, 2887
VneI	GTGCAC	1 cut(s)	1171
VpaK11BI	GGWCC	4 cut(s)	253, 280, 1954, 2540
XapI	RAATTY	3 cut(s)	706, 1618, 2105
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1717
XmiI	GTMKAC	1 cut(s)	204
XmnI	GAANNNNTTC	1 cut(s)	3077
XspI	CTAG	4 cut(s)	2166, 2601, 2756, 3014
ZrmI	AGTACT	1 cut(s)	953
Zsp2I	ATGCAT	1 cut(s)	1780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.