Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Reverse (-)

70480549 .. 70487162

Gene Structure 6614 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG407900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGGGAGAAACTGAGAAGTCGGCGGCCACCAAAACGGAGTCGTTTCCGGCGTGGTCGAAGGACGTGAAGGAATGCGAGGACGAGTTCAAGGTCAGCCGCGACTCGGGGCTGTCGGAGGAGGAAGTCGAGAAGCGCCGGGCGGTGTACGGATGGAACGAACTGGAGAAGCACGAGGACCAGTCGATCCTGAAGCTCCTGATGGATCAGCTCAGCGACACGCTCGTCAGGGTTCTGTTGTTCGCGGCGGTTATCTCCTTCGTTCTCGCATGGTACGAAGGCGAGGAGACCGGCGAGATGGACATCACCGCGTTCGTGGAGCCGTTGGTGATCTTTCTGATCCTGATCGTGAATGCGATCGTCGGAATCTGGCAGGAGAGCAACGCCGAGAAGGCGCTGGAGGCCTTGAAGGAGATTCAGTCGGAGCAGGCGGCGGTGGTCAGGGACGGGAAGAAGGTGCCGAATTTGGCGGCGAAGGAGCTCGTTCCGGGCGATATTGTGGAGCTGAGAGTCGGCGATAAGGTTCCGGCGGATATGAGGGTTTTGAGTTTGGTGAGCTCGACGGTGAGGATCGAGCAAGGGTCGTTGACCGGGGAGAGTGAGGCGGTTAGCAAGACGGCGAAGGCGGTGGCGGAGAATTCGGACATTCAGGGGAAGAAGTGTATGGTTTTCGCGGGAACGACGGTGGTGAACGGGAATTGCATGTGTTTGGTGACTCAGATTGGGATGAAGACGGAGATAGGGCAGGTGCACTCTCAGATTCAGGAGGCTTCACAGAGTGAGGAAGAGACTCCATTGAAGAAGAAGCTCAATGAGTTTGGGGAGATTTTGACTGCTATAATTGGTGTCATTTGTGCTTTGGTTTGGCTGATCAATCTCAAGTACTTTCTCAGCTGGGAAATGGTTGATGGGTGGCCGACGAATTTCGAATTTTCATTCGAGAGGTGTACTTATTACTTCAAGATTGCTGTGGCATTGGCCGTGGCGGCAATTCCAGAAGGCTTGCCTGCAGTGATCACCACCTGTTTGGCGCTTGGGACGAGGAAGATGGCTCAGAAGAATGCACTTGTTAGGAAGCTGCCAAGTGTGGAGACTCTAGGCTGTACTACTGTGATTTGTTCTGATAAGACCGGTACTCTAACTACTAATCAGATGGCTGTGTCGAAGCTTGTGGCTATTGGTACTAGGCCTGGTACTCTTCGAGCCTTTGATGTTGAGGGAACTACATACAACCCTTCTGATGGGAAAATAACAGACTGGCCGCCGGCTGGTCATATGGATACCAATGTTCAAATGATTGCGAAGATCTCTGCTCTGTGTAATGATGCTGGTGTTGAATTGTCAGGGAGTCATTATGTTGCCAGTGGAATGCCCACAGAGGCAGCCTTGAAGGTTCTGGTTGAGAAGATGGGAGTTCCTGAATCACTCAATACTTGTTTCAATTCAGGTTCGAAGGATATACTGCGTTGTTCTACACTTTGGAAGGAAGTTGATCGCCGGATTGCAACACTTGAGTTTGACCGTGACCGCAAATCCATGGGTGTAATTGTGAATACTGGCGCCGGAAAGAACTCTCTTTATGTCAAGGGTGCAGTTGAAAATGTATTGGAGCGAAGCTCATCCATTCAGTTGATTGATGGCTCCATTGCAGAATTGGACCAAAATTTCAAAGATCTTATCTTAGGCAGCCTTCATGAAATGTCTAGTAGTGCATTGCGCTGTTTGGGGTTTGCATACAAGGAGGACCTGCCAGAGTTCGCAGGGTATAATGGTGATGAAGACCATCCAGCTCATCAGCTTTTACTTGATCCTTCCAACTACTCTTCAATTGAGAGTAAACTTGTTTTTGTTGGCTTTGTTGGTCTAAGGGATCCTCCACGAAAAGAGGTCCGTCAAGCAATTGAGGACTGCAAAACTGCTGGAATCCGTGTTATGGTTATTACGGGAGATAACAAGAATACAGCTGAAGCTATTTGCCGCGAAATAGGTGTATTTGGACCTTCTGAAAATATCAGTTCGAGAAGCTTAACAGGGAAAGAATTCATACATCACCATGATCAGAAAAATCATTTGAGACAACTTGGAGGCCTTTTGTTTTCTAGAGCTGAACCAAGGCATAAACAAGAGATAGTGAGGCTGCTAAAGGAAGACGGTGAAGTGGTTGCTATGACTGGTGATGGAGTGAATGATGCACCTGCCTTGAAGTTGGCTGACATTGGGATTGCCATGGGCATTGCTGGGACAGAGGTTGCAAAAGAGGCCTCTGATATGGTATTAGCAGATGACAATTTTAGCACCATAGTTTCTGCTGTTGGAGAAGGCAGATCTATTTACAACAACATGAAAGCTTTTATCAGGTACATGATTTCATCAAACATTGGCGAAGTTGCCTCCATTTTTCTGACAGCGGCCCTGGGAATTCCAGAAGGCATGATTCCAGTTCAGCTTCTGTGGGTCAACCTGGTTACTGATGGACCCCCAGCAACAGCTCTAGGATTCAATCCTCCGGACAAAGATATAATGGAGAAGCCCCCTAGAAGAAGCAACGACTCATTGATCAGTGTGTGGATCTTATTTCGTTATCTGGTACAAGAACTATTAATATTTCCCCATCAAAAACATTTCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

875

Amino Acids

95.68

Weight (kDa)

5.11

Isoelectric Point (pI)

37.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	18 - 85	6.8e-19	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	138 - 253	2e-23	P-type ATPase actuator domain
Cation_ATPase	PF13246	429 - 628	3.3e-37	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	616 - 737	1.6e-15	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	809 - 867	8e-19	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	733, 2200
AasI	GACNNNNNNGTC	1 cut(s)	221
Acc36I	ACCTGC	3 cut(s)	733, 1752, 2200
AccB1I	GGYRCC	2 cut(s)	454, 1556
AccII	CGCG	5 cut(s)	99, 242, 308, 671, 1977
AccIII	TCCGGA	1 cut(s)	2503
AclWI	GGATC	8 cut(s)	178, 210, 331, 575, 1799, 1862, 1875, 2573
AcoI	YGGCCR	4 cut(s)	24, 911, 975, 1256
AcsI	RAATTY	7 cut(s)	460, 634, 919, 926, 1660, 2036, 2415
AcuI	CTGAAG	2 cut(s)	209, 1983
AcyI	GRCGYC	1 cut(s)	1557
AdeI	CACNNNGTG	1 cut(s)	776
AfiI	CCNNNNNNNGG	4 cut(s)	103, 1238, 1265, 1930
AgeI	ACCGGT	1 cut(s)	1127
AjiI	CACGTC	1 cut(s)	64
AjnI	CCWGG	3 cut(s)	1186, 2409, 2457
Alw21I	GWGCWC	3 cut(s)	480, 557, 750
Alw26I	GTCTC	4 cut(s)	278, 779, 1082, 2065
Alw44I	GTGCAC	1 cut(s)	746
AlwI	GGATC	8 cut(s)	178, 210, 331, 575, 1799, 1862, 1875, 2573
AlwNI	CAGNNNCTG	1 cut(s)	2446
Ama87I	CYCGRG	1 cut(s)	103
Aor13HI	TCCGGA	1 cut(s)	2503
AoxI	GGCC	9 cut(s)	24, 399, 911, 975, 1184, 1256, 2083, 2256, 2406
ApaLI	GTGCAC	1 cut(s)	746
ApeKI	GCWGC	4 cut(s)	1075, 1379, 1683, 2134
ApoI	RAATTY	7 cut(s)	460, 634, 919, 926, 1660, 2036, 2415
AseI	ATTAAT	1 cut(s)	2597
AsiGI	ACCGGT	1 cut(s)	1127
Asp700I	GAANNNNTTC	1 cut(s)	2036
AspLEI	GCGC	5 cut(s)	135, 394, 1030, 1559, 1716
AspS9I	GGNCC	7 cut(s)	175, 1654, 1741, 1885, 1994, 2407, 2471
AsuC2I	CCSGG	3 cut(s)	137, 486, 589
AsuII	TTCGAA	2 cut(s)	924, 1448
AvaI	CYCGRG	1 cut(s)	103
AvaII	GGWCC	6 cut(s)	175, 1654, 1741, 1885, 1994, 2471
BaeGI	GKGCMC	1 cut(s)	750
BaeI	ACNNNNGTAYC	2 cut(s)	1269, 1302
BamHI	GGATCC	1 cut(s)	1867
BanI	GGYRCC	2 cut(s)	454, 1556
BanII	GRGCYC	2 cut(s)	480, 557
BauI	CACGAG	1 cut(s)	170
BbsI	GAAGAC	3 cut(s)	734, 1782, 2151
Bbv12I	GWGCWC	3 cut(s)	480, 557, 750
BbvI	GCAGC	4 cut(s)	1062, 1391, 1695, 2121
BceAI	ACGGC	3 cut(s)	304, 630, 962
BciT130I	CCWGG	3 cut(s)	1188, 2411, 2459
BciVI	GTATCC	1 cut(s)	1270
BclI	TGATCA	4 cut(s)	867, 1011, 2053, 2553
BcnI	CCSGG	3 cut(s)	137, 486, 589
BcoDI	GTCTC	4 cut(s)	278, 779, 1082, 2065
BfaI	CTAG	6 cut(s)	1094, 1182, 1701, 2097, 2489, 2532
BfmI	CTRYAG	1 cut(s)	1005
BfoI	RGCGCY	4 cut(s)	136, 395, 1031, 1560
BfuAI	ACCTGC	3 cut(s)	733, 1752, 2200
BfuI	GTATCC	1 cut(s)	1270
BglI	GCCNNNNNGGC	2 cut(s)	389, 983
BglII	AGATCT	3 cut(s)	1302, 1669, 2321
BlpI	GCTNAGC	1 cut(s)	209
BmcAI	AGTACT	1 cut(s)	881
Bme1390I	CCNGG	6 cut(s)	137, 486, 589, 1188, 2411, 2459
Bme18I	GGWCC	6 cut(s)	175, 1654, 1741, 1885, 1994, 2471
BmeT110I	CYCGRG	1 cut(s)	103
BmgBI	CACGTC	1 cut(s)	64
BmgT120I	GGNCC	7 cut(s)	175, 1654, 1741, 1885, 1994, 2407, 2471
BmiI	GGNNCC	7 cut(s)	318, 456, 522, 1558, 1639, 1869, 2473
BmrFI	CCNGG	6 cut(s)	137, 486, 589, 1188, 2411, 2459
BmsI	GCATC	2 cut(s)	1312, 2176
BpiI	GAAGAC	3 cut(s)	734, 1782, 2151
BplI	GAGNNNNNCTC	2 cut(s)	1598, 1630
BpmI	CTGGAG	2 cut(s)	182, 416
Bpu1102I	GCTNAGC	1 cut(s)	209
Bpu14I	TTCGAA	2 cut(s)	924, 1448
BpuEI	CTTGAG	2 cut(s)	860, 1529
BpuMI	CCSGG	3 cut(s)	137, 486, 589
BsaBI	GATNNNNATC	2 cut(s)	299, 341
BsaHI	GRCGYC	1 cut(s)	1557
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	7 cut(s)	588, 978, 1533, 2108, 2223, 2409, 2410
BsaWI	WCCGGW	2 cut(s)	1127, 2503
BsaXI	ACNNNNNCTCC	2 cut(s)	401, 431
Bsc4I	CCNNNNNNNGG	4 cut(s)	103, 1238, 1265, 1930
Bse118I	RCCGGY	3 cut(s)	287, 1127, 1261
Bse1I	ACTGG	7 cut(s)	165, 178, 1259, 1359, 1558, 2173, 2435
Bse3DI	GCAATG	3 cut(s)	1641, 1709, 2229
Bse8I	GATNNNNATC	2 cut(s)	299, 341
BseAI	TCCGGA	1 cut(s)	2503
BseBI	CCWGG	3 cut(s)	1188, 2411, 2459
BseDI	CCNNGG	7 cut(s)	588, 978, 1533, 2108, 2223, 2409, 2410
BseGI	GGATG	4 cut(s)	155, 729, 1616, 1780
BseJI	GATNNNNATC	2 cut(s)	299, 341
BseLI	CCNNNNNNNGG	4 cut(s)	103, 1238, 1265, 1930
BseMI	GCAATG	3 cut(s)	1641, 1709, 2229
BseMII	CTCAG	6 cut(s)	223, 494, 728, 767, 901, 1064
BseNI	ACTGG	7 cut(s)	165, 178, 1259, 1359, 1558, 2173, 2435
BseRI	GAGGAG	2 cut(s)	131, 296
BseSI	GKGCMC	1 cut(s)	750
BseXI	GCAGC	4 cut(s)	1062, 1391, 1695, 2121
BseYI	CCCAGC	3 cut(s)	891, 2234, 2476
BsgI	GTGCAG	1 cut(s)	1608
Bsh1236I	CGCG	5 cut(s)	99, 242, 308, 671, 1977
Bsh1285I	CGRYCG	1 cut(s)	357
BshFI	GGCC	9 cut(s)	26, 401, 913, 977, 1186, 1258, 2085, 2258, 2408
BshNI	GGYRCC	2 cut(s)	454, 1556
BshTI	ACCGGT	1 cut(s)	1127
BsiEI	CGRYCG	1 cut(s)	357
BsiHKAI	GWGCWC	3 cut(s)	480, 557, 750
BsiHKCI	CYCGRG	1 cut(s)	103
BslFI	GGGAC	3 cut(s)	455, 1048, 2251
BslI	CCNNNNNNNGG	4 cut(s)	103, 1238, 1265, 1930
BsmAI	GTCTC	4 cut(s)	278, 779, 1082, 2065
BsmFI	GGGAC	3 cut(s)	455, 1048, 2251
BsmI	GAATGC	4 cut(s)	77, 355, 1063, 1371
BsnI	GGCC	9 cut(s)	26, 401, 913, 977, 1186, 1258, 2085, 2258, 2408
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	1 cut(s)	103
Bsp119I	TTCGAA	2 cut(s)	924, 1448
Bsp1286I	GDGCHC	3 cut(s)	480, 557, 750
Bsp13I	TCCGGA	1 cut(s)	2503
Bsp1720I	GCTNAGC	1 cut(s)	209
Bsp19I	CCATGG	2 cut(s)	1533, 2223
BspANI	GGCC	9 cut(s)	26, 401, 913, 977, 1186, 1258, 2085, 2258, 2408
BspCNI	CTCAG	7 cut(s)	4, 222, 495, 727, 766, 900, 1063
BspEI	TCCGGA	1 cut(s)	2503
BspFNI	CGCG	5 cut(s)	99, 242, 308, 671, 1977
BspHI	TCATGA	1 cut(s)	1690
BspLI	GGNNCC	7 cut(s)	318, 456, 522, 1558, 1639, 1869, 2473
BspMAI	CTGCAG	1 cut(s)	1009
BspMI	ACCTGC	3 cut(s)	733, 1752, 2200
BspPI	GGATC	8 cut(s)	178, 210, 331, 575, 1799, 1862, 1875, 2573
BspT104I	TTCGAA	2 cut(s)	924, 1448
BspT107I	GGYRCC	2 cut(s)	454, 1556
BspTNI	GGTCTC	1 cut(s)	278
BsrDI	GCAATG	3 cut(s)	1641, 1709, 2229
BsrFI	RCCGGY	3 cut(s)	287, 1127, 1261
BsrI	ACTGG	7 cut(s)	165, 178, 1259, 1359, 1558, 2173, 2435
BssAI	RCCGGY	3 cut(s)	287, 1127, 1261
BssECI	CCNNGG	7 cut(s)	588, 978, 1533, 2108, 2223, 2409, 2410
BssNI	GRCGYC	1 cut(s)	1557
BssSI	CACGAG	1 cut(s)	170
BssT1I	CCWWGG	3 cut(s)	1533, 2108, 2223
Bst2BI	CACGAG	1 cut(s)	170
Bst2UI	CCWGG	3 cut(s)	1188, 2411, 2459
Bst4CI	ACNGT	5 cut(s)	562, 682, 1108, 1520, 2150
Bst6I	CTCTTC	3 cut(s)	777, 1200, 1825
BstACI	GRCGYC	1 cut(s)	1557
BstBI	TTCGAA	2 cut(s)	924, 1448
BstC8I	GCNNGC	4 cut(s)	426, 1001, 1005, 1263
BstDEI	CTNAG	9 cut(s)	12, 209, 503, 714, 753, 887, 1050, 1678, 1862
BstDSI	CCRYGG	3 cut(s)	978, 1533, 2223
BstF5I	GGATG	4 cut(s)	155, 729, 1616, 1780
BstFNI	CGCG	5 cut(s)	99, 242, 308, 671, 1977
BstH2I	RGCGCY	4 cut(s)	136, 395, 1031, 1560
BstHHI	GCGC	5 cut(s)	135, 394, 1030, 1559, 1716
BstMAI	GTCTC	4 cut(s)	278, 779, 1082, 2065
BstMCI	CGRYCG	1 cut(s)	357
BstMWI	GCNNNNNNNGC	4 cut(s)	389, 398, 983, 2255
BstNI	CCWGG	3 cut(s)	1188, 2411, 2459
BstNSI	RCATGY	1 cut(s)	703
BstSCI	CCNGG	6 cut(s)	135, 484, 587, 1186, 2409, 2457
BstSFI	CTRYAG	1 cut(s)	1005
BstSLI	GKGCMC	1 cut(s)	750
BstUI	CGCG	5 cut(s)	99, 242, 308, 671, 1977
BstV1I	GCAGC	4 cut(s)	1062, 1391, 1695, 2121
BstV2I	GAAGAC	3 cut(s)	734, 1782, 2151
BstX2I	RGATCY	5 cut(s)	1302, 1669, 1867, 2321, 2565
BstXI	CCANNNNNNTGG	1 cut(s)	1024
BstYI	RGATCY	5 cut(s)	1302, 1669, 1867, 2321, 2565
BsuI	GTATCC	1 cut(s)	1270
BsuRI	GGCC	9 cut(s)	26, 401, 913, 977, 1186, 1258, 2085, 2258, 2408
BtgI	CCRYGG	3 cut(s)	978, 1533, 2223
BtrI	CACGTC	1 cut(s)	64
BtsCI	GGATG	4 cut(s)	155, 729, 1616, 1780
BtsI	GCAGTG	1 cut(s)	1014
BtsIMutI	CAGTG	3 cut(s)	1014, 1366, 2563
BveI	ACCTGC	3 cut(s)	733, 1752, 2200
Cac8I	GCNNGC	4 cut(s)	426, 1001, 1005, 1263
CaiI	CAGNNNCTG	1 cut(s)	2446
CciI	TCATGA	1 cut(s)	1690
CfoI	GCGC	5 cut(s)	135, 394, 1030, 1559, 1716
Cfr10I	RCCGGY	3 cut(s)	287, 1127, 1261
Cfr13I	GGNCC	7 cut(s)	175, 1654, 1741, 1885, 1994, 2407, 2471
CsiI	ACCWGGT	1 cut(s)	2457
CspAI	ACCGGT	1 cut(s)	1127
CviAII	CATG	9 cut(s)	267, 700, 1534, 1691, 2051, 2224, 2338, 2359, 2428
DdeI	CTNAG	9 cut(s)	12, 209, 503, 714, 753, 887, 1050, 1678, 1862
DinI	GGCGCC	1 cut(s)	1558
DraIII	CACNNNGTG	1 cut(s)	776
DrdI	GACNNNNNNGTC	1 cut(s)	221
DseDI	GACNNNNNNGTC	1 cut(s)	221
EaeI	YGGCCR	4 cut(s)	24, 911, 975, 1256
Eam1104I	CTCTTC	3 cut(s)	777, 1200, 1825
EarI	CTCTTC	3 cut(s)	777, 1200, 1825
EciI	GGCGGA	2 cut(s)	542, 644
Ecl136II	GAGCTC	2 cut(s)	478, 555
Eco130I	CCWWGG	3 cut(s)	1533, 2108, 2223
Eco147I	AGGCCT	4 cut(s)	401, 1186, 2085, 2258
Eco24I	GRGCYC	2 cut(s)	480, 557
Eco31I	GGTCTC	1 cut(s)	278
Eco47I	GGWCC	6 cut(s)	175, 1654, 1741, 1885, 1994, 2471
Eco53kI	GAGCTC	2 cut(s)	478, 555
Eco57I	CTGAAG	2 cut(s)	209, 1983
Eco88I	CYCGRG	1 cut(s)	103
EcoICRI	GAGCTC	2 cut(s)	478, 555
EcoO109I	RGGNCCY	1 cut(s)	1741
EcoRI	GAATTC	3 cut(s)	634, 2036, 2415
EcoRII	CCWGG	3 cut(s)	1186, 2409, 2457
EcoT14I	CCWWGG	3 cut(s)	1533, 2108, 2223
EcoT38I	GRGCYC	2 cut(s)	480, 557
EgeI	GGCGCC	1 cut(s)	1558
EheI	GGCGCC	1 cut(s)	1558
ErhI	CCWWGG	3 cut(s)	1533, 2108, 2223
FaeI	CATG	9 cut(s)	270, 703, 1537, 1694, 2054, 2227, 2341, 2362, 2431
FaqI	GGGAC	3 cut(s)	455, 1048, 2251
FatI	CATG	9 cut(s)	266, 699, 1533, 1690, 2050, 2223, 2337, 2358, 2427
FauI	CCCGC	1 cut(s)	664
FauNDI	CATATG	1 cut(s)	1272
FbaI	TGATCA	4 cut(s)	867, 1011, 2053, 2553
FokI	GGATG	4 cut(s)	162, 736, 1603, 1767
FriOI	GRGCYC	2 cut(s)	480, 557
FspBI	CTAG	6 cut(s)	1094, 1182, 1701, 2097, 2489, 2532
GlaI	GCGC	5 cut(s)	134, 393, 1029, 1558, 1715
GsaI	CCCAGC	3 cut(s)	895, 2238, 2480
GsuI	CTGGAG	2 cut(s)	182, 416
HaeII	RGCGCY	4 cut(s)	136, 395, 1031, 1560
HaeIII	GGCC	9 cut(s)	26, 401, 913, 977, 1186, 1258, 2085, 2258, 2408
HhaI	GCGC	5 cut(s)	135, 394, 1030, 1559, 1716
Hin1I	GRCGYC	1 cut(s)	1557
Hin1II	CATG	9 cut(s)	270, 703, 1537, 1694, 2054, 2227, 2341, 2362, 2431
Hin6I	GCGC	5 cut(s)	133, 392, 1028, 1557, 1714
HinP1I	GCGC	5 cut(s)	133, 392, 1028, 1557, 1714
HincII	GTYRAC	2 cut(s)	585, 2455
HindII	GTYRAC	2 cut(s)	585, 2455
HindIII	AAGCTT	3 cut(s)	1163, 2020, 2343
Hpy166II	GTNNAC	7 cut(s)	145, 585, 688, 748, 945, 1835, 2455
Hpy8I	GTNNAC	7 cut(s)	145, 585, 688, 748, 945, 1835, 2455
Hpy99I	CGWCG	4 cut(s)	362, 562, 682, 919
HpyCH4III	ACNGT	5 cut(s)	562, 682, 1108, 1520, 2150
HpyCH4IV	ACGT	1 cut(s)	63
HpyF10VI	GCNNNNNNNGC	4 cut(s)	389, 398, 983, 2255
HpyF3I	CTNAG	9 cut(s)	12, 209, 503, 714, 753, 887, 1050, 1678, 1862
HpySE526I	ACGT	1 cut(s)	63
Hsp92I	GRCGYC	1 cut(s)	1557
Hsp92II	CATG	9 cut(s)	270, 703, 1537, 1694, 2054, 2227, 2341, 2362, 2431
HspAI	GCGC	5 cut(s)	133, 392, 1028, 1557, 1714
KasI	GGCGCC	1 cut(s)	1556
Kpn2I	TCCGGA	1 cut(s)	2503
KroI	GCCGGC	1 cut(s)	1261
KroNI	GCCGGC	1 cut(s)	1263
Ksp22I	TGATCA	4 cut(s)	867, 1011, 2053, 2553
LmnI	GCTCC	7 cut(s)	198, 316, 421, 475, 499, 1606, 1643
Lsp1109I	GCAGC	4 cut(s)	1062, 1391, 1695, 2121
LweI	GCATC	2 cut(s)	1312, 2176
MabI	ACCWGGT	1 cut(s)	2457
MaeI	CTAG	6 cut(s)	1094, 1182, 1701, 2097, 2489, 2532
MaeII	ACGT	1 cut(s)	63
MaeIII	GTNAC	3 cut(s)	709, 1520, 2461
MfeI	CAATTG	2 cut(s)	1824, 1896
MflI	RGATCY	5 cut(s)	1302, 1669, 1867, 2321, 2565
MhlI	GDGCHC	3 cut(s)	480, 557, 750
Mly113I	GGCGCC	1 cut(s)	1557
MlyI	GAGTC	8 cut(s)	47, 95, 516, 706, 781, 1084, 1354, 2540
MmeI	TCCRAC	5 cut(s)	93, 340, 399, 1836, 2290
MroI	TCCGGA	1 cut(s)	2503
MroNI	GCCGGC	1 cut(s)	1261
MroXI	GAANNNNTTC	1 cut(s)	2036
MseI	TTAA	2 cut(s)	2024, 2597
MslI	CAYNNNNRTG	1 cut(s)	2049
MspA1I	CMGCKG	3 cut(s)	891, 1961, 2405
MspR9I	CCNGG	6 cut(s)	137, 486, 589, 1188, 2411, 2459
MunI	CAATTG	2 cut(s)	1824, 1896
Mva1269I	GAATGC	4 cut(s)	77, 355, 1063, 1371
MvaI	CCWGG	3 cut(s)	1188, 2411, 2459
MvnI	CGCG	5 cut(s)	99, 242, 308, 671, 1977
MwoI	GCNNNNNNNGC	4 cut(s)	389, 398, 983, 2255
NaeI	GCCGGC	1 cut(s)	1263
NarI	GGCGCC	1 cut(s)	1557
NciI	CCSGG	3 cut(s)	137, 486, 589
NcoI	CCATGG	2 cut(s)	1533, 2223
NdeI	CATATG	1 cut(s)	1272
NgoMIV	GCCGGC	1 cut(s)	1261
NlaIII	CATG	9 cut(s)	270, 703, 1537, 1694, 2054, 2227, 2341, 2362, 2431
NlaIV	GGNNCC	7 cut(s)	318, 456, 522, 1558, 1639, 1869, 2473
NmeAIII	GCCGAG	1 cut(s)	409
NmuCI	GTSAC	2 cut(s)	709, 1520
NspI	RCATGY	1 cut(s)	703
NspV	TTCGAA	2 cut(s)	924, 1448
PagI	TCATGA	1 cut(s)	1690
PaqCI	CACCTGC	2 cut(s)	733, 2200
PasI	CCCWGGG	1 cut(s)	2410
PceI	AGGCCT	4 cut(s)	401, 1186, 2085, 2258
PcsI	WCGNNNNNNNCGW	4 cut(s)	47, 153, 270, 486
PctI	GAATGC	4 cut(s)	77, 355, 1063, 1371
PdiI	GCCGGC	1 cut(s)	1263
PdmI	GAANNNNTTC	1 cut(s)	2036
PfeI	GAWTC	7 cut(s)	363, 412, 757, 1418, 1920, 2431, 2493
PinAI	ACCGGT	1 cut(s)	1127
Ple19I	CGATCG	1 cut(s)	357
PleI	GAGTC	8 cut(s)	46, 95, 515, 706, 781, 1084, 1353, 2540
PluTI	GGCGCC	1 cut(s)	1560
PpsI	GAGTC	8 cut(s)	46, 95, 515, 706, 781, 1084, 1353, 2540
PpuMI	RGGWCCY	1 cut(s)	1741
PshBI	ATTAAT	1 cut(s)	2597
Psp124BI	GAGCTC	2 cut(s)	480, 557
Psp5II	RGGWCCY	1 cut(s)	1741
Psp6I	CCWGG	3 cut(s)	1186, 2409, 2457
PspFI	CCCAGC	3 cut(s)	891, 2234, 2476
PspGI	CCWGG	3 cut(s)	1186, 2409, 2457
PspN4I	GGNNCC	7 cut(s)	318, 456, 522, 1558, 1639, 1869, 2473
PspPI	GGNCC	7 cut(s)	175, 1654, 1741, 1885, 1994, 2407, 2471
PspPPI	RGGWCCY	1 cut(s)	1741
PstI	CTGCAG	1 cut(s)	1009
PstNI	CAGNNNCTG	1 cut(s)	2446
PsuI	RGATCY	5 cut(s)	1302, 1669, 1867, 2321, 2565
PvuI	CGATCG	1 cut(s)	357
PvuII	CAGCTG	2 cut(s)	891, 1961
RseI	CAYNNNNRTG	1 cut(s)	2049
SacI	GAGCTC	2 cut(s)	480, 557
SaqAI	TTAA	2 cut(s)	2024, 2597
Sau96I	GGNCC	7 cut(s)	175, 1654, 1741, 1885, 1994, 2407, 2471
ScaI	AGTACT	1 cut(s)	881
SchI	GAGTC	8 cut(s)	47, 95, 516, 706, 781, 1084, 1354, 2540
ScrFI	CCNGG	6 cut(s)	137, 486, 589, 1188, 2411, 2459
SduI	GDGCHC	3 cut(s)	480, 557, 750
SexAI	ACCWGGT	1 cut(s)	2457
SfaNI	GCATC	2 cut(s)	1312, 2176
SfcI	CTRYAG	1 cut(s)	1005
SfoI	GGCGCC	1 cut(s)	1558
SfuI	TTCGAA	2 cut(s)	924, 1448
SinI	GGWCC	6 cut(s)	175, 1654, 1741, 1885, 1994, 2471
SmiMI	CAYNNNNRTG	1 cut(s)	2049
SmlI	CTYRAG	2 cut(s)	875, 1508
SmoI	CTYRAG	2 cut(s)	875, 1508
SseBI	AGGCCT	4 cut(s)	401, 1186, 2085, 2258
SspDI	GGCGCC	1 cut(s)	1556
SspI	AATATT	1 cut(s)	2601
SspMI	CTAG	6 cut(s)	1094, 1182, 1701, 2097, 2489, 2532
SstI	GAGCTC	2 cut(s)	480, 557
StuI	AGGCCT	4 cut(s)	401, 1186, 2085, 2258
StyD4I	CCNGG	6 cut(s)	135, 484, 587, 1186, 2409, 2457
StyI	CCWWGG	3 cut(s)	1533, 2108, 2223
TaaI	ACNGT	5 cut(s)	562, 682, 1108, 1520, 2150
TaiI	ACGT	1 cut(s)	66
TatI	WGTACW	3 cut(s)	879, 944, 1100
TauI	GCSGC	9 cut(s)	26, 99, 245, 431, 470, 986, 1261, 1977, 2408
TfiI	GAWTC	7 cut(s)	363, 412, 757, 1418, 1920, 2431, 2493
Tru1I	TTAA	2 cut(s)	2024, 2597
Tru9I	TTAA	2 cut(s)	2024, 2597
TscAI	CASTG	3 cut(s)	1014, 1366, 2563
TseFI	GTSAC	2 cut(s)	709, 1520
TseI	GCWGC	4 cut(s)	1075, 1379, 1683, 2134
Tsp45I	GTSAC	2 cut(s)	709, 1520
TspDTI	ATGAA	8 cut(s)	740, 921, 1679, 1707, 1788, 2029, 2354, 2355
TspGWI	ACGGA	5 cut(s)	50, 162, 746, 1877, 1913
TspRI	CASTG	3 cut(s)	1014, 1366, 2563
VneI	GTGCAC	1 cut(s)	746
VpaK11BI	GGWCC	6 cut(s)	175, 1654, 1741, 1885, 1994, 2471
VspI	ATTAAT	1 cut(s)	2597
XapI	RAATTY	7 cut(s)	460, 634, 919, 926, 1660, 2036, 2415
XbaI	TCTAGA	1 cut(s)	2096
XceI	RCATGY	1 cut(s)	703
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1648
XmnI	GAANNNNTTC	1 cut(s)	2036
XspI	CTAG	6 cut(s)	1094, 1182, 1701, 2097, 2489, 2532
ZrmI	AGTACT	1 cut(s)	881

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.