Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001873

Physical Location & Seq

Genomic Coordinates Reverse (-)

20111992 .. 20118068

Gene Structure 6077 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1116400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3192 bp

ATGGGGAAGGGAGGGCAGGATTTCGGTAAACGGAAAGAGGACGGAAACCCTAGGCCGGTGGACGGCGATGTGTTTCCGGCGTGGGCGAAGGAGATTTCGGAGTGCGAGAAGCACTTTGGGGTTAACAGAAAATTAGGGTTGGGATCGGAGGACGTCGAGAAGCGGCGGGAGAAGTACGGATCGAACGAGTTGGAGAAGCACGAGGGGCAGTCGATTTGGAGTCTGGTTCTGGAGCAGTTCAATGATACTCTGGTTCGAATCTTGCTTGCGGCGGCGGTCATTTCTTTCGTTCTGGCTTGGCTGGATGGGGAAGAAGGCGGTGAGAAGGAGATCACGGCGTTTGTGGAGCCGCTGGTGATCTTCTTGATCTTGATTGTGAACGCGATTGTAGGGGTTTGGCAGGAGACCAATGCCGAGAAGGCCTTGGAGGCGCTGAAGGAGATTCAATCGGAGCAGGCGACTGTGATTCGGAATGGGAGTAAGGTTCCGAATTTGCCGGCGAAGGAGCTTGTTCCTGGTGACATTGTGGAGCTTAAGGTTGGGGATAAGGTGCCCGCTGATATGCGTGTTGTGGAACTGATTAGCTCAACTTTGAGGGTGGAGCAGGGTTCATTGACCGGAGAGAGTGAGGCGGTGAATAAGACTAACAAGCCGGTCTCTGAGGACGCAGATATTCAAGGGAAGAAAAATATGGTTTTCGCTGGGACGACTATTGTGAATGGACATTGCATATGCTTGGTTGCACAGACAGGAATGTCGACTGAAATTGGGAAGGTGCATATGCAAATCCATGTCGCTGCGCAGATCGAAGAAGATACACCACTTAAGAAGAAGCTTAATGAGTTTGGAGAGATGCTTACAATGATAATTGGAGTGATTTGCATATTAGTGTGGCTCATCAATGTCAAGTACTTCCTTACTTGGGAATATGTCAATGGAAGGCCGGCAAATTTCAAGTTTTCGTTTGAGAAGTGCACATATTACTTTGAGATTGCAGTAGCATTGGCTGTGGCTGCTATTCCTGAAGGTCTGCCTGCAGTCATTACAACGTGCTTGGCGCTTGGTACCCGTAAAATGGCTCAGAAGAATGCTTTGGTCCGAAAGCTGCCTAGTGTTGAAACTCTGGGTTGTACAACTGTGATTTGTTCGGATAAAACAGGGACCCTGACTACAAACCAGATGGCAGTGGCAAAACTTGTAGCTTTGGGTCCTAGACCTACTATACTACGGAAGTTTAGGGTGGATGGAACTACATACAATCCACTTGATGGTAAAATTAATGACTGGCCGACTGGTCGTATGGATGCTAATCTTCAAATGATCGCAAAGGTAGCTGCTGTCTGCAATGATGCAGGCATTACCCAGGCAGAACAGAAGTATGTTGCCCATGGAATGCCTACCGAGGCAGCTCTAAAGGTTTTAGTTGAGAAGATGGGCCTTCCCGAAGGATCCAAGTGTGCGGAATCATCAAATGAAAGGGATTTGCTTGGTTGTTGCCAGAAGTGGAGTGAATTGGAATGCCGAATTGCAACCCTTGAGTTTGATCGTGATAGAAAATCCATGGGTGTAATTGTCAATTCCCGTTCAGGAAAGAAGTCATTGCTAGTGAAGGGTGCTGTAGAGAATTTGCTGGAGAGAAGCACTAAGGTTCAGTTGCTTGATGGCACTGTTGTACCTCTGGATGATAGCTCAAGGAACTATATCATGCAAGCTCTTCATGAGATGTCAACTAGTGCACTGCGCTGTTTGGGTTTTGCATACAAAGATGAGCTTGGTGAGTTTTCATCCTATGATGGTGATGAAGATCATCCAGCTCATAGACTTTTACTTGATCCATCCAATTATTCGTCAATTGAGAGCGAACTTGTTTTTGTTGGCCTTGTTGGACTGAGGGATCCGCCAAGAGAGGAGGTTTTTGATGCAATTGAAGACTGCCGAGCAGCAGGAATTCGTGTTATGGTAATCACTGGAGATAACCAGAACACAGCAGAAGCAATATGCCGTGAAATAGGCGTGTTTGGTGCTGATGAAGACATACGTTCAAGAAGCCTTACAGGAAAAGAGTTTATGGGTCTACGTGATCAGAAAGCCCATCTAAGACAAGGTGGTGGCCTTCTGTTTTCAAGGGCCGAACCAAAGCACAAGCAAGAGATTGTGAGGTTGCTCAAAGAGGATGGTGAGGTGGTTGCTATGACTGGTGATGGAGTGAATGATGCACCTGCTTTGAAACTTGCTGATATTGGGATTGCAATGGGCATTTCTGGAACTGAGGTTGCAAAGGAAGCTTCTGACATGGTTTTGGCTGATGATAATTTTAGTACAATTGTTGCAGCTGTTGGTGAAGGCAGATCTATCTACAATAACATGAAAGCCTTTATCAGGTACATGATCTCGTCCAACATTGGTGAGGTTGCCTCCATTTTCTTAACAGCAGCATTAGGCATTCCTGAAGGCCTCATACCTGTTCAGCTCTTGTGGGTGAATCTTGTTACTGATGGACCACCTGCAACAGCACTAGGATTTAATCCTCCAGACAAAGACATAATGAAGAAGCCCCCACGCAGAAGCGATGATTCACTTATCAGCGCCTGGATATTATTCCGCTATATGGTTATTGGGATGTATGTTGGACTGGCAACTGTAGGCGTATTCATCATTTGGTATACACATGGTTCCTTCCTTGGTATTGACCTCAGTGGGGATGGCCACTCCCTTGTGACTTACTCCCAACTTGCTAACTGGGGACAGTGCTCATCTTGGACAAATTTCACCGCTTCACCCTTCACAGCCGGAAATGAGGTCCTCTCCTTTGATAACCCCTGTGATTATTTCCATGCTGGCAAAGTGAAAGCTATGACACTATCCCTCTCAGTGTTGGTCGCCATCGAAATGTTCAACTCTCTCAATGCCCTTTCTGAGGACGGAAGCCTGCTGACCATGCCCCCATGGGTCAACCCCTGGCTTCTGGTGGCCATGTCTGTTTCATTCGGCCTGCACTTCTTGATCCTGTATGTGCCATTCTTGGCTCAAGTATTTGGCATTGTGCCCCTGAGCATAAACGAGTGGCTTCTGGTTTTGGCCGTTTCACTCCCAGTGATTCTTATTGACGAGGTCCTCAAGCTTGTTGGAAGATGGACAAGCGCTTCTCAAATAACCAGGAGGACAAAACCATCAAGGCCCAAGACAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0010035 GO:0010038 GO:0010042 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042221 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1064

Amino Acids

116.26

Weight (kDa)

5.3

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	28 - 95	1e-19	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	148 - 260	1.5e-24	P-type ATPase actuator domain
Cation_ATPase	PF13246	438 - 637	5.4e-40	P-type ATPase, cytoplasmic domain N
HAD	PF12710	553 - 743	3.9e-06	haloacid dehalogenase-like hydrolase
Hydrolase	PF00702	625 - 746	1.2e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	818 - 1042	1.8e-48	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001873)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07670 AT1G07670 AT1G07810
fragaria_vesca	FvH4_3g34570 FvH4_3g34570 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760 FvH4_6g14760
malus_domestica	MD04G1038900.v1.1 MD04G1116400.v1.1 MD11G1117700.v1.1 MD12G1132100.v1.1 MD13G1142700.v1.1
prunus_persica	Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1 Prupe.6G246300_v2.0.a1
pyrus_communis	pycom03g12390 pycom03g12400 pycom04g10640 pycom07g10780 pycom11g09870 pycom12g12810 pycom16g18850
rosa_chinensis	RchiOBHm_Chr3g0467981 RchiOBHm_Chr5g0062231
rosa_laevigata	RLG00000024462 RLG00000035545
rosa_multiflora	Rmu_co8021382.1_g000001 Rmu_sc0001793.1_g000060 Rmu_ssc0000409.1_g000035
rosa_roxburghii	Rroxscaffold_1G00018460 Rroxscaffold_6G00413240
rosa_rugosa	Rorug03G0090700 Rorug03G0090800 Rorug05G0347300
rosa_samantha	Rh3AG139600 Rh3BG161100 Rh3CG161000 Rh3DG161500 Rh5AG407900 Rh5BG421600 Rh5CG445600 Rh5DG436600
rosa_wichuraiana	Rw3G013200 Rw5G038440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2227, 2512
AasI	GACNNNNNNGTC	1 cut(s)	754
AatII	GACGTC	1 cut(s)	156
Acc16I	TGCGCA	1 cut(s)	801
Acc36I	ACCTGC	2 cut(s)	2227, 2512
Acc65I	GGTACC	1 cut(s)	1064
AccB1I	GGYRCC	2 cut(s)	550, 1064
AccB7I	CCANNNNNTGG	1 cut(s)	1268
AccI	GTMKAC	3 cut(s)	758, 2074, 2663
AccII	CGCG	1 cut(s)	383
AclWI	GGATC	8 cut(s)	151, 187, 1443, 1456, 1826, 1889, 1902, 2998
AcoI	YGGCCR	4 cut(s)	1286, 2704, 2970, 3078
AcsI	RAATTY	5 cut(s)	490, 949, 1624, 1947, 2764
AcuI	CTGAAG	3 cut(s)	455, 1044, 2469
AcyI	GRCGYC	1 cut(s)	153
AfaI	GTAC	7 cut(s)	176, 911, 1066, 1132, 1674, 2320, 2384
AfeI	AGCGCT	1 cut(s)	3142
AfiI	CCNNNNNNNGG	9 cut(s)	55, 62, 922, 1075, 1268, 2379, 2608, 2698, 2917
AflII	CTTAAG	2 cut(s)	533, 824
AhlI	ACTAGT	1 cut(s)	1730
AjnI	CCWGG	5 cut(s)	514, 1362, 2588, 2957, 3155
AjuI	GAANNNNNNNTTGG	2 cut(s)	1076, 1108
Alw21I	GWGCWC	3 cut(s)	977, 1738, 2753
Alw26I	GTCTC	2 cut(s)	398, 661
Alw44I	GTGCAC	2 cut(s)	973, 1734
AlwI	GGATC	8 cut(s)	151, 187, 1443, 1456, 1826, 1889, 1902, 2998
AlwNI	CAGNNNCTG	1 cut(s)	2589
Aor51HI	AGCGCT	1 cut(s)	3142
ApaLI	GTGCAC	2 cut(s)	973, 1734
ApeKI	GCWGC	8 cut(s)	797, 1013, 1105, 1334, 1406, 1940, 2330, 2432
ApoI	RAATTY	5 cut(s)	490, 949, 1624, 1947, 2764
AseI	ATTAAT	1 cut(s)	1278
Asp718I	GGTACC	1 cut(s)	1064
AspA2I	CCTAGG	1 cut(s)	50
AspLEI	GCGC	6 cut(s)	433, 802, 1060, 1743, 2588, 3143
AspS9I	GGNCC	9 cut(s)	1096, 1161, 1208, 1435, 2127, 2498, 2800, 3112, 3177
AsuII	TTCGAA	1 cut(s)	256
AvaII	GGWCC	6 cut(s)	1096, 1161, 1208, 2498, 2800, 3112
AvrII	CCTAGG	1 cut(s)	50
BaeGI	GKGCMC	4 cut(s)	555, 977, 1738, 3048
BaeI	ACNNNNGTAYC	4 cut(s)	237, 270, 1656, 1689
BalI	TGGCCA	2 cut(s)	2706, 2972
BamHI	GGATCC	2 cut(s)	1448, 1894
BanI	GGYRCC	2 cut(s)	550, 1064
BarI	GAAGNNNNNNTAC	2 cut(s)	2442, 2474
BauI	CACGAG	1 cut(s)	200
BbsI	GAAGAC	2 cut(s)	1935, 2037
Bbv12I	GWGCWC	3 cut(s)	977, 1738, 2753
BbvI	GCAGC	8 cut(s)	784, 1000, 1092, 1321, 1418, 1952, 2342, 2444
BceAI	ACGGC	4 cut(s)	79, 351, 1986, 3065
BciT130I	CCWGG	5 cut(s)	516, 1364, 2590, 2959, 3157
BclI	TGATCA	1 cut(s)	2080
BcoDI	GTCTC	2 cut(s)	398, 661
BcuI	ACTAGT	1 cut(s)	1730
BfaI	CTAG	6 cut(s)	51, 1110, 1212, 1604, 1731, 2516
BfmI	CTRYAG	3 cut(s)	1035, 1617, 2640
BfoI	RGCGCY	4 cut(s)	434, 1061, 2589, 3144
BfrI	CTTAAG	2 cut(s)	533, 824
BfuAI	ACCTGC	2 cut(s)	2227, 2512
BglI	GCCNNNNNGGC	2 cut(s)	419, 428
BglII	AGATCT	1 cut(s)	2348
BlnI	CCTAGG	1 cut(s)	50
BmcAI	AGTACT	1 cut(s)	911
Bme1390I	CCNGG	5 cut(s)	516, 1364, 2590, 2959, 3157
Bme18I	GGWCC	6 cut(s)	1096, 1161, 1208, 2498, 2800, 3112
BmgT120I	GGNCC	9 cut(s)	1096, 1161, 1208, 1435, 2127, 2498, 2800, 3112, 3177
BmrFI	CCNGG	5 cut(s)	516, 1364, 2590, 2959, 3157
BmrI	ACTGGG	2 cut(s)	2749, 3086
BmsI	GCATC	5 cut(s)	843, 1294, 1339, 1909, 2203
BmuI	ACTGGG	2 cut(s)	2749, 3086
BpiI	GAAGAC	2 cut(s)	1935, 2037
BplI	GAGNNNNNCTC	4 cut(s)	2399, 2431, 2789, 2821
BpmI	CTGGAG	4 cut(s)	251, 1652, 1989, 2514
Bpu10I	CCTNAGC	1 cut(s)	3050
Bpu14I	TTCGAA	1 cut(s)	256
BpuEI	CTTGAG	4 cut(s)	1556, 1675, 3012, 3101
BsaAI	YACGTR	1 cut(s)	2078
BsaBI	GATNNNNATC	2 cut(s)	1308, 1803
BsaHI	GRCGYC	1 cut(s)	153
BsaI	GGTCTC	2 cut(s)	398, 661
BsaJI	CCNNGG	9 cut(s)	50, 423, 1362, 1387, 1401, 1560, 2680, 2945, 2957
BsaWI	WCCGGW	1 cut(s)	617
Bsc4I	CCNNNNNNNGG	9 cut(s)	55, 62, 922, 1075, 1268, 2379, 2608, 2698, 2917
Bse118I	RCCGGY	4 cut(s)	55, 496, 652, 943
Bse1I	ACTGG	7 cut(s)	1289, 1297, 1972, 2200, 2637, 2744, 3092
Bse3DI	GCAATG	4 cut(s)	724, 1351, 1598, 2256
Bse8I	GATNNNNATC	2 cut(s)	1308, 1803
BseBI	CCWGG	5 cut(s)	516, 1364, 2590, 2959, 3157
BseDI	CCNNGG	9 cut(s)	50, 423, 1362, 1387, 1401, 1560, 2680, 2945, 2957
BseJI	GATNNNNATC	2 cut(s)	1308, 1803
BseLI	CCNNNNNNNGG	9 cut(s)	55, 62, 922, 1075, 1268, 2379, 2608, 2698, 2917
BseMI	GCAATG	4 cut(s)	724, 1351, 1598, 2256
BseMII	CTCAG	8 cut(s)	651, 1094, 1880, 2259, 2707, 2883, 2907, 3041
BseNI	ACTGG	7 cut(s)	1289, 1297, 1972, 2200, 2637, 2744, 3092
BseRI	GAGGAG	1 cut(s)	1922
BseSI	GKGCMC	4 cut(s)	555, 977, 1738, 3048
BseXI	GCAGC	8 cut(s)	784, 1000, 1092, 1321, 1418, 1952, 2342, 2444
BseYI	CCCAGC	1 cut(s)	701
BsgI	GTGCAG	1 cut(s)	2978
Bsh1236I	CGCG	1 cut(s)	383
BshNI	GGYRCC	2 cut(s)	550, 1064
BsiHKAI	GWGCWC	3 cut(s)	977, 1738, 2753
BsiSI	CCGG	7 cut(s)	56, 77, 497, 618, 653, 944, 2790
BslFI	GGGAC	3 cut(s)	718, 1174, 2757
BslI	CCNNNNNNNGG	9 cut(s)	55, 62, 922, 1075, 1268, 2379, 2608, 2698, 2917
BsmAI	GTCTC	2 cut(s)	398, 661
BsmFI	GGGAC	3 cut(s)	718, 1174, 2757
BsmI	GAATGC	4 cut(s)	1093, 1398, 1523, 2442
Bso31I	GGTCTC	2 cut(s)	398, 661
Bsp119I	TTCGAA	1 cut(s)	256
Bsp1286I	GDGCHC	5 cut(s)	555, 977, 1738, 2753, 3048
Bsp1407I	TGTACA	1 cut(s)	1130
Bsp19I	CCATGG	3 cut(s)	1387, 1560, 2945
BspCNI	CTCAG	8 cut(s)	652, 1093, 1881, 2260, 2706, 2882, 2908, 3042
BspFNI	CGCG	1 cut(s)	383
BspHI	TCATGA	1 cut(s)	1717
BspMAI	CTGCAG	1 cut(s)	1039
BspMI	ACCTGC	2 cut(s)	2227, 2512
BspPI	GGATC	8 cut(s)	151, 187, 1443, 1456, 1826, 1889, 1902, 2998
BspQI	GCTCTTC	1 cut(s)	1719
BspT104I	TTCGAA	1 cut(s)	256
BspT107I	GGYRCC	2 cut(s)	550, 1064
BspTI	CTTAAG	2 cut(s)	533, 824
BspTNI	GGTCTC	2 cut(s)	398, 661
BsrDI	GCAATG	4 cut(s)	724, 1351, 1598, 2256
BsrFI	RCCGGY	4 cut(s)	55, 496, 652, 943
BsrGI	TGTACA	1 cut(s)	1130
BsrI	ACTGG	7 cut(s)	1289, 1297, 1972, 2200, 2637, 2744, 3092
BssAI	RCCGGY	4 cut(s)	55, 496, 652, 943
BssECI	CCNNGG	9 cut(s)	50, 423, 1362, 1387, 1401, 1560, 2680, 2945, 2957
BssNAI	GTATAC	1 cut(s)	2664
BssNI	GRCGYC	1 cut(s)	153
BssSI	CACGAG	1 cut(s)	200
BssT1I	CCWWGG	6 cut(s)	50, 423, 1387, 1560, 2680, 2945
Bst1107I	GTATAC	1 cut(s)	2664
Bst2BI	CACGAG	1 cut(s)	200
Bst2UI	CCWGG	5 cut(s)	516, 1364, 2590, 2959, 3157
Bst4CI	ACNGT	5 cut(s)	463, 1138, 1669, 2641, 2748
Bst6I	CTCTTC	1 cut(s)	1719
BstACI	GRCGYC	1 cut(s)	153
BstAFI	CTTAAG	2 cut(s)	533, 824
BstAUI	TGTACA	1 cut(s)	1130
BstBAI	YACGTR	1 cut(s)	2078
BstBI	TTCGAA	1 cut(s)	256
BstDSI	CCRYGG	3 cut(s)	1387, 1560, 2945
BstENI	CCTNNNNNAGG	2 cut(s)	2377, 2915
BstFNI	CGCG	1 cut(s)	383
BstH2I	RGCGCY	4 cut(s)	434, 1061, 2589, 3144
BstHHI	GCGC	6 cut(s)	433, 802, 1060, 1743, 2588, 3143
BstMAI	GTCTC	2 cut(s)	398, 661
BstMWI	GCNNNNNNNGC	7 cut(s)	205, 419, 428, 1013, 1331, 2282, 2938
BstNI	CCWGG	5 cut(s)	516, 1364, 2590, 2959, 3157
BstSCI	CCNGG	5 cut(s)	514, 1362, 2588, 2957, 3155
BstSFI	CTRYAG	3 cut(s)	1035, 1617, 2640
BstSLI	GKGCMC	4 cut(s)	555, 977, 1738, 3048
BstUI	CGCG	1 cut(s)	383
BstV1I	GCAGC	8 cut(s)	784, 1000, 1092, 1321, 1418, 1952, 2342, 2444
BstV2I	GAAGAC	2 cut(s)	1935, 2037
BstX2I	RGATCY	3 cut(s)	1448, 1894, 2348
BstYI	RGATCY	3 cut(s)	1448, 1894, 2348
BstZ17I	GTATAC	1 cut(s)	2664
BtgI	CCRYGG	3 cut(s)	1387, 1560, 2945
BtgZI	GCGATG	2 cut(s)	81, 2583
BtsI	GCAGTG	2 cut(s)	1191, 1736
BtsIMutI	CAGTG	8 cut(s)	1191, 1665, 1736, 1965, 2701, 2753, 2877, 3099
BveI	ACCTGC	2 cut(s)	2227, 2512
CaiI	CAGNNNCTG	1 cut(s)	2589
CciI	TCATGA	1 cut(s)	1717
CfoI	GCGC	6 cut(s)	433, 802, 1060, 1743, 2588, 3143
Cfr10I	RCCGGY	4 cut(s)	55, 496, 652, 943
Cfr13I	GGNCC	9 cut(s)	1096, 1161, 1208, 1435, 2127, 2498, 2800, 3112, 3177
CseI	GACGC	1 cut(s)	674
Csp6I	GTAC	7 cut(s)	175, 910, 1065, 1131, 1673, 2319, 2383
CviQI	GTAC	7 cut(s)	175, 910, 1065, 1131, 1673, 2319, 2383
DrdI	GACNNNNNNGTC	1 cut(s)	754
DseDI	GACNNNNNNGTC	1 cut(s)	754
EaeI	YGGCCR	4 cut(s)	1286, 2704, 2970, 3078
Eam1104I	CTCTTC	1 cut(s)	1719
EarI	CTCTTC	1 cut(s)	1719
EciI	GGCGGA	1 cut(s)	1887
Eco130I	CCWWGG	6 cut(s)	50, 423, 1387, 1560, 2680, 2945
Eco147I	AGGCCT	2 cut(s)	422, 2454
Eco31I	GGTCTC	2 cut(s)	398, 661
Eco47I	GGWCC	6 cut(s)	1096, 1161, 1208, 2498, 2800, 3112
Eco47III	AGCGCT	1 cut(s)	3142
Eco57I	CTGAAG	3 cut(s)	455, 1044, 2469
EcoNI	CCTNNNNNAGG	2 cut(s)	2377, 2915
EcoO109I	RGGNCCY	4 cut(s)	1161, 1208, 2800, 3112
EcoRI	GAATTC	1 cut(s)	1947
EcoRII	CCWGG	5 cut(s)	514, 1362, 2588, 2957, 3155
EcoT14I	CCWWGG	6 cut(s)	50, 423, 1387, 1560, 2680, 2945
ErhI	CCWWGG	6 cut(s)	50, 423, 1387, 1560, 2680, 2945
FalI	AAGNNNNNCTT	2 cut(s)	1755, 1787
FaqI	GGGAC	3 cut(s)	718, 1174, 2757
FauI	CCCGC	2 cut(s)	159, 562
FauNDI	CATATG	2 cut(s)	731, 780
FbaI	TGATCA	1 cut(s)	2080
FblI	GTMKAC	3 cut(s)	758, 2074, 2663
FspBI	CTAG	6 cut(s)	51, 1110, 1212, 1604, 1731, 2516
FspI	TGCGCA	1 cut(s)	801
GlaI	GCGC	6 cut(s)	432, 801, 1059, 1742, 2587, 3142
GsaI	CCCAGC	1 cut(s)	705
GsuI	CTGGAG	4 cut(s)	251, 1652, 1989, 2514
HaeII	RGCGCY	4 cut(s)	434, 1061, 2589, 3144
HapII	CCGG	7 cut(s)	56, 77, 497, 618, 653, 944, 2790
HgaI	GACGC	1 cut(s)	674
HhaI	GCGC	6 cut(s)	433, 802, 1060, 1743, 2588, 3143
Hin1I	GRCGYC	1 cut(s)	153
Hin6I	GCGC	6 cut(s)	431, 800, 1058, 1741, 2586, 3141
HinP1I	GCGC	6 cut(s)	431, 800, 1058, 1741, 2586, 3141
HincII	GTYRAC	4 cut(s)	124, 759, 1728, 2953
HindII	GTYRAC	4 cut(s)	124, 759, 1728, 2953
HindIII	AAGCTT	3 cut(s)	833, 2283, 3119
HinfI	GANTC	8 cut(s)	220, 258, 442, 466, 1463, 2482, 2573, 3097
HpaI	GTTAAC	1 cut(s)	124
HpaII	CCGG	7 cut(s)	56, 77, 497, 618, 653, 944, 2790
Hpy99I	CGWCG	1 cut(s)	158
HpyCH4III	ACNGT	5 cut(s)	463, 1138, 1669, 2641, 2748
HpyCH4IV	ACGT	4 cut(s)	153, 1049, 2038, 2077
HpyF10VI	GCNNNNNNNGC	7 cut(s)	205, 419, 428, 1013, 1331, 2282, 2938
HpySE526I	ACGT	4 cut(s)	153, 1049, 2038, 2077
Hsp92I	GRCGYC	1 cut(s)	153
HspAI	GCGC	6 cut(s)	431, 800, 1058, 1741, 2586, 3141
KflI	GGGWCCC	1 cut(s)	1161
KpnI	GGTACC	1 cut(s)	1068
KroI	GCCGGC	2 cut(s)	496, 943
KroNI	GCCGGC	2 cut(s)	498, 945
Ksp22I	TGATCA	1 cut(s)	2080
KspAI	GTTAAC	1 cut(s)	124
LguI	GCTCTTC	1 cut(s)	1719
LmnI	GCTCC	6 cut(s)	232, 346, 451, 505, 529, 601
Lsp1109I	GCAGC	8 cut(s)	784, 1000, 1092, 1321, 1418, 1952, 2342, 2444
LweI	GCATC	5 cut(s)	843, 1294, 1339, 1909, 2203
MaeI	CTAG	6 cut(s)	51, 1110, 1212, 1604, 1731, 2516
MaeII	ACGT	4 cut(s)	153, 1049, 2038, 2077
MaeIII	GTNAC	3 cut(s)	518, 2488, 2716
MfeI	CAATTG	3 cut(s)	1851, 1923, 2322
MflI	RGATCY	3 cut(s)	1448, 1894, 2348
MhlI	GDGCHC	5 cut(s)	555, 977, 1738, 2753, 3048
MlsI	TGGCCA	2 cut(s)	2706, 2972
MluNI	TGGCCA	2 cut(s)	2706, 2972
MlyI	GAGTC	1 cut(s)	229
MmeI	TCCRAC	5 cut(s)	171, 1864, 2421, 2608, 3106
Mox20I	TGGCCA	2 cut(s)	2706, 2972
MroNI	GCCGGC	2 cut(s)	496, 943
MscI	TGGCCA	2 cut(s)	2706, 2972
MseI	TTAA	7 cut(s)	123, 534, 825, 837, 1278, 2426, 2523
MslI	CAYNNNNRTG	2 cut(s)	887, 2888
Msp20I	TGGCCA	2 cut(s)	2706, 2972
MspA1I	CMGCKG	3 cut(s)	352, 557, 2333
MspCI	CTTAAG	2 cut(s)	533, 824
MspI	CCGG	7 cut(s)	56, 77, 497, 618, 653, 944, 2790
MspR9I	CCNGG	5 cut(s)	516, 1364, 2590, 2959, 3157
MunI	CAATTG	3 cut(s)	1851, 1923, 2322
Mva1269I	GAATGC	4 cut(s)	1093, 1398, 1523, 2442
MvaI	CCWGG	5 cut(s)	516, 1364, 2590, 2959, 3157
MvnI	CGCG	1 cut(s)	383
MwoI	GCNNNNNNNGC	7 cut(s)	205, 419, 428, 1013, 1331, 2282, 2938
NaeI	GCCGGC	2 cut(s)	498, 945
NcoI	CCATGG	3 cut(s)	1387, 1560, 2945
NdeI	CATATG	2 cut(s)	731, 780
NgoMIV	GCCGGC	2 cut(s)	496, 943
NmeAIII	GCCGAG	2 cut(s)	439, 1961
NmuCI	GTSAC	2 cut(s)	518, 2716
NsbI	TGCGCA	1 cut(s)	801
NspV	TTCGAA	1 cut(s)	256
PagI	TCATGA	1 cut(s)	1717
PaqCI	CACCTGC	2 cut(s)	2227, 2512
PceI	AGGCCT	2 cut(s)	422, 2454
PciSI	GCTCTTC	1 cut(s)	1719
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 455
PctI	GAATGC	4 cut(s)	1093, 1398, 1523, 2442
PdiI	GCCGGC	2 cut(s)	498, 945
PfeI	GAWTC	7 cut(s)	258, 442, 466, 1463, 2482, 2573, 3097
PflFI	GACNNNGTC	1 cut(s)	3110
PflMI	CCANNNNNTGG	1 cut(s)	1268
PleI	GAGTC	1 cut(s)	228
PpsI	GAGTC	1 cut(s)	228
Ppu21I	YACGTR	1 cut(s)	2078
PpuMI	RGGWCCY	4 cut(s)	1161, 1208, 2800, 3112
PshBI	ATTAAT	1 cut(s)	1278
Psp5II	RGGWCCY	4 cut(s)	1161, 1208, 2800, 3112
Psp6I	CCWGG	5 cut(s)	514, 1362, 2588, 2957, 3155
PspFI	CCCAGC	1 cut(s)	701
PspGI	CCWGG	5 cut(s)	514, 1362, 2588, 2957, 3155
PspPI	GGNCC	9 cut(s)	1096, 1161, 1208, 1435, 2127, 2498, 2800, 3112, 3177
PspPPI	RGGWCCY	4 cut(s)	1161, 1208, 2800, 3112
PstI	CTGCAG	1 cut(s)	1039
PstNI	CAGNNNCTG	1 cut(s)	2589
PsuI	RGATCY	3 cut(s)	1448, 1894, 2348
PsyI	GACNNNGTC	1 cut(s)	3110
PvuII	CAGCTG	1 cut(s)	2333
RsaI	GTAC	7 cut(s)	176, 911, 1066, 1132, 1674, 2320, 2384
RsaNI	GTAC	7 cut(s)	175, 910, 1065, 1131, 1673, 2319, 2383
RseI	CAYNNNNRTG	2 cut(s)	887, 2888
SalI	GTCGAC	1 cut(s)	757
SapI	GCTCTTC	1 cut(s)	1719
SaqAI	TTAA	7 cut(s)	123, 534, 825, 837, 1278, 2426, 2523
Sau96I	GGNCC	9 cut(s)	1096, 1161, 1208, 1435, 2127, 2498, 2800, 3112, 3177
ScaI	AGTACT	1 cut(s)	911
SchI	GAGTC	1 cut(s)	229
ScrFI	CCNGG	5 cut(s)	516, 1364, 2590, 2959, 3157
SduI	GDGCHC	5 cut(s)	555, 977, 1738, 2753, 3048
SfaNI	GCATC	5 cut(s)	843, 1294, 1339, 1909, 2203
SfcI	CTRYAG	3 cut(s)	1035, 1617, 2640
SfuI	TTCGAA	1 cut(s)	256
SinI	GGWCC	6 cut(s)	1096, 1161, 1208, 2498, 2800, 3112
SmiMI	CAYNNNNRTG	2 cut(s)	887, 2888
SmlI	CTYRAG	6 cut(s)	533, 824, 1535, 1690, 3027, 3116
SmoI	CTYRAG	6 cut(s)	533, 824, 1535, 1690, 3027, 3116
SpeI	ACTAGT	1 cut(s)	1730
SseBI	AGGCCT	2 cut(s)	422, 2454
SspMI	CTAG	6 cut(s)	51, 1110, 1212, 1604, 1731, 2516
StuI	AGGCCT	2 cut(s)	422, 2454
StyD4I	CCNGG	5 cut(s)	514, 1362, 2588, 2957, 3155
StyI	CCWWGG	6 cut(s)	50, 423, 1387, 1560, 2680, 2945
TaaI	ACNGT	5 cut(s)	463, 1138, 1669, 2641, 2748
TaiI	ACGT	4 cut(s)	156, 1052, 2041, 2080
TaqI	TCGA	7 cut(s)	156, 182, 212, 256, 758, 807, 2886
TatI	WGTACW	3 cut(s)	909, 1130, 2318
TauI	GCSGC	4 cut(s)	166, 272, 275, 352
TfiI	GAWTC	7 cut(s)	258, 442, 466, 1463, 2482, 2573, 3097
Tru1I	TTAA	7 cut(s)	123, 534, 825, 837, 1278, 2426, 2523
Tru9I	TTAA	7 cut(s)	123, 534, 825, 837, 1278, 2426, 2523
TscAI	CASTG	8 cut(s)	1191, 1672, 1743, 1972, 2701, 2753, 2877, 3099
TseFI	GTSAC	2 cut(s)	518, 2716
TseI	GCWGC	8 cut(s)	797, 1013, 1105, 1334, 1406, 1940, 2330, 2432
Tsp45I	GTSAC	2 cut(s)	518, 2716
TspGWI	ACGGA	5 cut(s)	46, 57, 192, 1243, 2937
TspRI	CASTG	8 cut(s)	1191, 1672, 1743, 1972, 2701, 2753, 2877, 3099
Tth111I	GACNNNGTC	1 cut(s)	3110
Van91I	CCANNNNNTGG	1 cut(s)	1268
Vha464I	CTTAAG	2 cut(s)	533, 824
VneI	GTGCAC	2 cut(s)	973, 1734
VpaK11BI	GGWCC	6 cut(s)	1096, 1161, 1208, 2498, 2800, 3112
VspI	ATTAAT	1 cut(s)	1278
XagI	CCTNNNNNAGG	2 cut(s)	2377, 2915
XapI	RAATTY	5 cut(s)	490, 949, 1624, 1947, 2764
XmaJI	CCTAGG	1 cut(s)	50
XmiI	GTMKAC	3 cut(s)	758, 2074, 2663
XspI	CTAG	6 cut(s)	51, 1110, 1212, 1604, 1731, 2516
ZraI	GACGTC	1 cut(s)	154
ZrmI	AGTACT	1 cut(s)	911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.