FvH4_6g16600

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004386
Physical Location & Seq
Forward (+)
10524248 .. 10527714
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16600.t2

Sequence Viewer

Length: 1239 bp
ATGGGTATTTTACATGACGACGTCGTAATCATCACAGAGCCAGAGAAAGAAGGCGACCCAAGTGTGATTACCATAAATTGCCCAGACAAAACTGGCTTGGGCTGTGACTTGTGTCGCATCATTCTCTTCTTTGGTCTTAGCATTGTCAGAGGAGATGTATCAACGGATGGGAAGTGGTGCTTCTTAGTGTTTTGGGTGGTTGGGAACCCAGGAACAAGGTGGGGTTTGTTGAAGAAGAGGTTAATGGAGACTTGTCCTTCTTTTTCTTCAGCTTCTGGGATTTCCTATTACCGCCATGAATTGCAGTCCCCAAAGACTCCAGATGTGTTCCTTCTCAAGTTCTGTTGTTATGATCGGAAAGGGATTTTACATGATGTGACTGGGGTTCTATGTGAACTAGAGCTAACCATAAAGAAAGTCAAGGTATCCACCACCCCCGACGAGAAAGTGATGGATCTGTTTTTCGTCACAGACACCAGGGAACTTCTGCATACAAATAAGAGAAAAGACGAGGTATCTGACCACTTAAAAGCTGCTACGGGCAATGCCATGATAAGTTGTGATATTGAAATGGTCGGTCCTGAGATTACTGCATGTTCACAGTCGTCTTCATTTCTCCCATCCGTTCTTATGGAAGACATGTTTCATTTGGAAATGCCTGATGAACTCCCAAGTGGACTAGTCACCTCTAGCTGTGTTTCCGTAAATATGGATAATTCGCTCAGTCCTGGCCACACGCTGGTCCAGATCATGTGCAAAGATCACAAGGGCCTCCTTTATGATATAATGAGAACTCTAAAGGATTATAATATACAGATCTCTTACGGCCGCTTCTCTTCGAAACAAAGAAGAAACTGCGAGATTGACTTGTTCATTGTGCAAGCTGATGGAAAGAAGATGGTTGATCCAAGCAAGCAGAATGCGTTGAAATCTCGTTTACAGATGGAACTACTCCGTCCTCTCAGAGTAGCTGTTGTAAGCAAAGGTCCTGATACAGAGCTCCTGGTTGCTAATCCTGTGGAATTATCTGGGAAGGGCCGGCCTCTTGTTTTTCATGATATAACCCTTGCTCTCAAGATGTCGAACACTGGCATATTTTCGGCTAAAATTGGAAGGCATTTAGTTAAAGATCGGGAATGGGAAGTCTATCGAGTCTTGCTTGATGAAGGAGATGGATTGCCAGTTCCAAGGAACAAGATTGAGGAAGGAGTATGGAAGATGTTGATGGGTTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

413

Amino Acids

46.23

Weight (kDa)

6.1

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 12 - 86 9.3e-39 ACT domain-containing protein ACR10, N-terminal
ACT PF01842 112 - 167 3.1e-06 ACT domain
ACT_ACR9_3rd PF24931 234 - 331 4.6e-49 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 332 - 368 2.6e-16 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AatII GACGTC 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 739
AciI CCGC 2 cut(s) 292, 829
AclWI GGATC 2 cut(s) 462, 899
AcoI YGGCCR 2 cut(s) 730, 826
AcuI CTGAAG 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 739
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 3 cut(s) 232, 569, 928
AhlI ACTAGT 1 cut(s) 679
AjnI CCWGG 4 cut(s) 208, 476, 727, 1002
AloI GAACNNNNNNTCC 2 cut(s) 1167, 1199
AluBI AGCT 7 cut(s) 272, 403, 533, 693, 884, 971, 1000
AluI AGCT 7 cut(s) 272, 403, 533, 693, 884, 971, 1000
Alw21I GWGCWC 1 cut(s) 1002
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 2 cut(s) 462, 899
AlwNI CAGNNNCTG 1 cut(s) 275
AoxI GGCC 5 cut(s) 730, 769, 826, 1036, 1040
ApeKI GCWGC 1 cut(s) 533
AspS9I GGNCC 5 cut(s) 578, 742, 769, 986, 1036
AsuHPI GGTGA 1 cut(s) 676
AsuII TTCGAA 1 cut(s) 839
AvaII GGWCC 3 cut(s) 578, 742, 986
BalI TGGCCA 1 cut(s) 732
BanII GRGCYC 1 cut(s) 1002
BbsI GAAGAC 2 cut(s) 600, 642
Bbv12I GWGCWC 1 cut(s) 1002
BbvI GCAGC 1 cut(s) 520
BccI CCATC 8 cut(s) 161, 445, 628, 881, 892, 937, 1166, 1219
BceAI ACGGC 1 cut(s) 841
BciT130I CCWGG 4 cut(s) 210, 478, 729, 1004
BciVI GTATCC 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 242
BcuI ACTAGT 1 cut(s) 679
BfaI CTAG 3 cut(s) 398, 680, 690
BfuI GTATCC 1 cut(s) 436
BglII AGATCT 1 cut(s) 816
BisI GCNGC 2 cut(s) 534, 829
BlsI GCNGC 2 cut(s) 535, 830
Bme1390I CCNGG 4 cut(s) 210, 478, 729, 1004
Bme18I GGWCC 3 cut(s) 578, 742, 986
BmgT120I GGNCC 5 cut(s) 578, 742, 769, 986, 1036
BmiI GGNNCC 1 cut(s) 206
BmrFI CCNGG 4 cut(s) 210, 478, 729, 1004
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 390
BoxI GACNNNNGTC 1 cut(s) 111
BpiI GAAGAC 2 cut(s) 600, 642
BpmI CTGGAG 1 cut(s) 303
Bpu14I TTCGAA 1 cut(s) 839
BpuEI CTTGAG 2 cut(s) 320, 1058
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 208, 477, 1187
Bsc4I CCNNNNNNNGG 1 cut(s) 739
Bse118I RCCGGY 1 cut(s) 1038
Bse1I ACTGG 4 cut(s) 97, 385, 1093, 1181
Bse3DI GCAATG 1 cut(s) 550
BseBI CCWGG 4 cut(s) 210, 478, 729, 1004
BseDI CCNNGG 3 cut(s) 208, 477, 1187
BseGI GGATG 2 cut(s) 172, 620
BseLI CCNNNNNNNGG 1 cut(s) 739
BseMI GCAATG 1 cut(s) 550
BseMII CTCAG 3 cut(s) 573, 736, 976
BseNI ACTGG 4 cut(s) 97, 385, 1093, 1181
BseRI GAGGAG 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 826
BseXI GCAGC 1 cut(s) 520
Bsh1285I CGRYCG 1 cut(s) 829
BshFI GGCC 5 cut(s) 732, 771, 828, 1038, 1042
BsiEI CGRYCG 1 cut(s) 829
BsiHKAI GWGCWC 1 cut(s) 1002
BsiSI CCGG 1 cut(s) 1039
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 739
BsmAI GTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 292
BsmI GAATGC 1 cut(s) 925
BsnI GGCC 5 cut(s) 732, 771, 828, 1038, 1042
Bsp119I TTCGAA 1 cut(s) 839
Bsp1286I GDGCHC 1 cut(s) 1002
Bsp143I GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
BspACI CCGC 2 cut(s) 292, 829
BspANI GGCC 5 cut(s) 732, 771, 828, 1038, 1042
BspCNI CTCAG 3 cut(s) 574, 735, 975
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 1 cut(s) 206
BspPI GGATC 2 cut(s) 462, 899
BspT104I TTCGAA 1 cut(s) 839
BsrDI GCAATG 1 cut(s) 550
BsrFI RCCGGY 1 cut(s) 1038
BsrI ACTGG 4 cut(s) 97, 385, 1093, 1181
BssAI RCCGGY 1 cut(s) 1038
BssECI CCNNGG 3 cut(s) 208, 477, 1187
BssMI GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 1187
Bst2UI CCWGG 4 cut(s) 210, 478, 729, 1004
Bst4CI ACNGT 1 cut(s) 603
Bst6I CTCTTC 3 cut(s) 131, 230, 841
BstACI GRCGYC 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 839
BstC8I GCNNGC 3 cut(s) 882, 914, 1040
BstDEI CTNAG 5 cut(s) 137, 184, 582, 722, 962
BstF5I GGATG 2 cut(s) 172, 620
BstKTI GATC 7 cut(s) 355, 457, 750, 763, 819, 907, 1132
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
BstMCI CGRYCG 1 cut(s) 829
BstNI CCWGG 4 cut(s) 210, 478, 729, 1004
BstNSI RCATGY 2 cut(s) 597, 643
BstPAI GACNNNNGTC 1 cut(s) 111
BstSCI CCNGG 4 cut(s) 208, 476, 727, 1002
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 2 cut(s) 600, 642
BstX2I RGATCY 2 cut(s) 454, 816
BstYI RGATCY 2 cut(s) 454, 816
BstZI CGGCCG 1 cut(s) 826
BsuI GTATCC 1 cut(s) 436
BsuRI GGCC 5 cut(s) 732, 771, 828, 1038, 1042
BtsCI GGATG 2 cut(s) 172, 620
BtsIMutI CAGTG 1 cut(s) 1086
Cac8I GCNNGC 3 cut(s) 882, 914, 1040
CaiI CAGNNNCTG 1 cut(s) 275
CciI TCATGA 1 cut(s) 1054
Cfr10I RCCGGY 1 cut(s) 1038
Cfr13I GGNCC 5 cut(s) 578, 742, 769, 986, 1036
CviAII CATG 8 cut(s) 14, 296, 371, 550, 594, 640, 751, 1055
DdeI CTNAG 5 cut(s) 137, 184, 582, 722, 962
DpnI GATC 7 cut(s) 354, 456, 749, 762, 818, 906, 1131
DpnII GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
EaeI YGGCCR 2 cut(s) 730, 826
EagI CGGCCG 1 cut(s) 826
Eam1104I CTCTTC 3 cut(s) 131, 230, 841
EarI CTCTTC 3 cut(s) 131, 230, 841
Ecl136II GAGCTC 1 cut(s) 1000
EclXI CGGCCG 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 1187
Eco24I GRGCYC 1 cut(s) 1002
Eco47I GGWCC 3 cut(s) 578, 742, 986
Eco52I CGGCCG 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 1000
Eco57I CTGAAG 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 1000
EcoO109I RGGNCCY 2 cut(s) 769, 986
EcoRII CCWGG 4 cut(s) 208, 476, 727, 1002
EcoT14I CCWWGG 1 cut(s) 1187
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 1 cut(s) 1187
FaeI CATG 8 cut(s) 17, 299, 374, 553, 597, 643, 754, 1058
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FaqI GGGAC 1 cut(s) 292
FatI CATG 8 cut(s) 13, 295, 370, 549, 593, 639, 750, 1054
Fnu4HI GCNGC 2 cut(s) 534, 829
FokI GGATG 2 cut(s) 179, 607
FriOI GRGCYC 1 cut(s) 1002
FseI GGCCGGCC 1 cut(s) 1042
Fsp4HI GCNGC 2 cut(s) 534, 829
FspBI CTAG 3 cut(s) 398, 680, 690
GluI GCNGC 2 cut(s) 534, 829
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 5 cut(s) 732, 771, 828, 1038, 1042
HapII CCGG 1 cut(s) 1039
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 8 cut(s) 17, 299, 374, 553, 597, 643, 754, 1058
HinfI GANTC 2 cut(s) 316, 1152
HpaII CCGG 1 cut(s) 1039
HphI GGTGA 1 cut(s) 676
Hpy166II GTNNAC 4 cut(s) 395, 599, 677, 938
Hpy188I TCNGA 4 cut(s) 149, 357, 520, 965
Hpy188III TCNNGA 7 cut(s) 320, 581, 745, 989, 1055, 1075, 1133
Hpy8I GTNNAC 4 cut(s) 395, 599, 677, 938
Hpy99I CGWCG 3 cut(s) 23, 26, 443
HpyAV CCTTC 7 cut(s) 44, 267, 341, 1027, 1107, 1160, 1199
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 5 cut(s) 304, 490, 593, 756, 880
HpyF3I CTNAG 5 cut(s) 137, 184, 582, 722, 962
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 8 cut(s) 17, 299, 374, 553, 597, 643, 754, 1058
KroI GCCGGC 1 cut(s) 1038
KroNI GCCGGC 1 cut(s) 1040
Kzo9I GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
LmnI GCTCC 1 cut(s) 1005
Lsp1109I GCAGC 1 cut(s) 520
LweI GCATC 1 cut(s) 126
MaeI CTAG 3 cut(s) 398, 680, 690
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 4 cut(s) 104, 376, 466, 682
MalI GATC 7 cut(s) 354, 456, 749, 762, 818, 906, 1131
MboI GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
MflI RGATCY 2 cut(s) 454, 816
MhlI GDGCHC 1 cut(s) 1002
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 5 cut(s) 76, 299, 715, 1022, 1107
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 2 cut(s) 310, 1161
MnlI CCTC 8 cut(s) 143, 231, 505, 697, 782, 969, 1053, 1195
Mox20I TGGCCA 1 cut(s) 732
MroNI GCCGGC 1 cut(s) 1038
MscI TGGCCA 1 cut(s) 732
MseI TTAA 3 cut(s) 242, 527, 1125
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 1 cut(s) 1039
MspR9I CCNGG 4 cut(s) 210, 478, 729, 1004
Mva1269I GAATGC 1 cut(s) 925
MvaI CCWGG 4 cut(s) 210, 478, 729, 1004
NaeI GCCGGC 1 cut(s) 1040
NdeII GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
NgoMIV GCCGGC 1 cut(s) 1038
NlaIII CATG 8 cut(s) 17, 299, 374, 553, 597, 643, 754, 1058
NlaIV GGNNCC 1 cut(s) 206
NmuCI GTSAC 4 cut(s) 104, 376, 466, 682
NspI RCATGY 2 cut(s) 597, 643
NspV TTCGAA 1 cut(s) 839
PagI TCATGA 1 cut(s) 1054
PciI ACATGT 1 cut(s) 639
PctI GAATGC 1 cut(s) 925
PdiI GCCGGC 1 cut(s) 1040
PflFI GACNNNGTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 739
PkrI GCNGC 2 cut(s) 535, 830
PleI GAGTC 2 cut(s) 310, 1160
PpsI GAGTC 2 cut(s) 310, 1160
PpuMI RGGWCCY 1 cut(s) 986
PscI ACATGT 1 cut(s) 639
PshAI GACNNNNGTC 1 cut(s) 111
PsiI TTATAA 1 cut(s) 807
Psp124BI GAGCTC 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 4 cut(s) 208, 476, 727, 1002
PspGI CCWGG 4 cut(s) 208, 476, 727, 1002
PspN4I GGNNCC 1 cut(s) 206
PspPI GGNCC 5 cut(s) 578, 742, 769, 986, 1036
PspPPI RGGWCCY 1 cut(s) 986
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 2 cut(s) 454, 816
PsyI GACNNNGTC 1 cut(s) 20
RigI GGCCGGCC 1 cut(s) 1042
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 3 cut(s) 242, 527, 1125
SatI GCNGC 2 cut(s) 534, 829
Sau3AI GATC 7 cut(s) 352, 454, 747, 760, 816, 904, 1129
Sau96I GGNCC 5 cut(s) 578, 742, 769, 986, 1036
SchI GAGTC 2 cut(s) 310, 1161
ScrFI CCNGG 4 cut(s) 210, 478, 729, 1004
SduI GDGCHC 1 cut(s) 1002
SfaNI GCATC 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 839
SinI GGWCC 3 cut(s) 578, 742, 986
SmlI CTYRAG 2 cut(s) 335, 1073
SmoI CTYRAG 2 cut(s) 335, 1073
SpeI ACTAGT 1 cut(s) 679
Sse9I AATT 5 cut(s) 76, 299, 715, 1022, 1107
SsiI CCGC 2 cut(s) 292, 829
SspMI CTAG 3 cut(s) 398, 680, 690
SstI GAGCTC 1 cut(s) 1002
StyD4I CCNGG 4 cut(s) 208, 476, 727, 1002
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 1 cut(s) 603
TaiI ACGT 1 cut(s) 24
TaqI TCGA 3 cut(s) 839, 1082, 1150
TaqII GACCGA 1 cut(s) 566
TasI AATT 5 cut(s) 76, 299, 715, 1022, 1107
TauI GCSGC 1 cut(s) 831
Tru1I TTAA 3 cut(s) 242, 527, 1125
Tru9I TTAA 3 cut(s) 242, 527, 1125
TscAI CASTG 1 cut(s) 1093
TseFI GTSAC 4 cut(s) 104, 376, 466, 682
TseI GCWGC 1 cut(s) 533
Tsp45I GTSAC 4 cut(s) 104, 376, 466, 682
TspDTI ATGAA 7 cut(s) 312, 600, 635, 678, 862, 1043, 1179
TspGWI ACGGA 4 cut(s) 179, 613, 691, 944
TspRI CASTG 1 cut(s) 1093
Tth111I GACNNNGTC 1 cut(s) 20
Van91I CCANNNNNTGG 1 cut(s) 739
VpaK11BI GGWCC 3 cut(s) 578, 742, 986
XceI RCATGY 2 cut(s) 597, 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 216
XspI CTAG 3 cut(s) 398, 680, 690
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.