Rorug01G0297700

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004386
Physical Location & Seq
Reverse (-)
41322545 .. 41324621
2077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0297700.1

Sequence Viewer

Length: 741 bp
ATGGCTCTTCTCGTTCGGTCACCGGAAATCTCCATAGTCTTCTCTAACCCTAACCGTAAAGGCTTTCGATTTCCGACGAACTTCGGGTCGAAATCCAGGTCCACGGTTAGGGTTTGCGCCGGGGTTAAGGACAGTGCGGTGAGGGCGTCGGAGTTGGATCGGAATTTAAGGTCGCACGGGCAGTTCTCGGCTCCGGTGAAGCAGGGTCCGAAGCCGAGCAAGGAAGATCAAGAGAAGCAGGAGTACTATGTGAATATGGGGTATGCGATTCGGACTGTGAGGGAGGAGTTTCCTGAGCTGTTTTTCAGAGAGCTTAGCCTCGACATTTACAGGGATGATATAACATTCAAAGATCCGATCAATACCTTTATGGGCATTGAGAATTACAAGTCTATATTTTCGGCACTACGTTTCCATGGCCAGATATTTTTCAAGGCCTTGTGGGTTGACGTCATTAGTGTATCGCAGCCGATGGACAATGTTATAATGGTTCGCTGGACTATCCATGGCATGCCACGAGTCCCTTGGGAAAGTCGTGGTCGATTTGATGGAACCTCAGAGTACAAACTTGACAAGAAAGGGAAGATCTATGAGCATCGTGTTGATAACATTGCTCTGAATTCGCCTCCAAAGTTTCAGATTCTGGCCGTAGGCGATATAATGCAATCTCTTGGGTGCCCTTCAACTCCAAAGCCAACATTTTTTGAAACTTCATCATTGCCATCTTCAAAGAGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

28.17

Weight (kDa)

9.56

Isoelectric Point (pI)

49.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 89 - 199 1.3e-38 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 485
AatII GACGTC 1 cut(s) 453
AccB1I GGYRCC 1 cut(s) 675
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 165, 347
AcoI YGGCCR 2 cut(s) 418, 645
AcsI RAATTY 2 cut(s) 163, 619
AcyI GRCGYC 2 cut(s) 146, 450
AfaI GTAC 2 cut(s) 245, 563
AfiI CCNNNNNNNGG 1 cut(s) 108
AgsI TTSAA 5 cut(s) 349, 433, 684, 707, 729
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 2 cut(s) 298, 313
AluI AGCT 2 cut(s) 298, 313
AlwI GGATC 2 cut(s) 165, 347
AlwNI CAGNNNCTG 1 cut(s) 643
AoxI GGCC 3 cut(s) 418, 435, 645
ApeKI GCWGC 1 cut(s) 466
ApoI RAATTY 2 cut(s) 163, 619
ArsI GACNNNNNNTTYG 4 cut(s) 71, 83, 103, 115
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 99, 206
AsuC2I CCSGG 1 cut(s) 121
AsuHPI GGTGA 3 cut(s) 12, 151, 208
AvaII GGWCC 2 cut(s) 99, 206
BaeGI GKGCMC 1 cut(s) 680
BalI TGGCCA 1 cut(s) 420
BanI GGYRCC 1 cut(s) 675
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BauI CACGAG 1 cut(s) 516
BbsI GAAGAC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 478
BccI CCATC 3 cut(s) 466, 542, 730
BceAI ACGGC 1 cut(s) 632
BciT130I CCWGG 1 cut(s) 97
BcnI CCSGG 1 cut(s) 121
BglII AGATCT 1 cut(s) 585
BisI GCNGC 1 cut(s) 467
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 468
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 2 cut(s) 97, 121
Bme18I GGWCC 2 cut(s) 99, 206
BmgT120I GGNCC 2 cut(s) 99, 206
BmiI GGNNCC 4 cut(s) 192, 207, 553, 677
BmrFI CCNGG 2 cut(s) 97, 121
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 294
Bpu1102I GCTNAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 121
BsaHI GRCGYC 2 cut(s) 146, 450
BsaJI CCNNGG 5 cut(s) 102, 120, 415, 505, 524
BsaWI WCCGGW 2 cut(s) 22, 193
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse3DI GCAATG 2 cut(s) 609, 716
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 5 cut(s) 102, 120, 415, 505, 524
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 2 cut(s) 609, 716
BseMII CTCAG 2 cut(s) 285, 570
BseRI GAGGAG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 680
BseXI GCAGC 1 cut(s) 478
BshFI GGCC 3 cut(s) 420, 437, 647
BshNI GGYRCC 1 cut(s) 675
BsiSI CCGG 3 cut(s) 23, 120, 194
BslFI GGGAC 1 cut(s) 506
BslI CCNNNNNNNGG 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 506
BsnI GGCC 3 cut(s) 420, 437, 647
Bsp1286I GDGCHC 1 cut(s) 680
Bsp143I GATC 5 cut(s) 157, 226, 352, 357, 585
Bsp1720I GCTNAGC 1 cut(s) 314
Bsp19I CCATGG 2 cut(s) 415, 505
BspACI CCGC 1 cut(s) 137
BspANI GGCC 3 cut(s) 420, 437, 647
BspCNI CTCAG 2 cut(s) 286, 569
BspLI GGNNCC 4 cut(s) 192, 207, 553, 677
BspPI GGATC 2 cut(s) 165, 347
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 675
BsrDI GCAATG 2 cut(s) 609, 716
BssECI CCNNGG 5 cut(s) 102, 120, 415, 505, 524
BssMI GATC 5 cut(s) 157, 226, 352, 357, 585
BssNI GRCGYC 2 cut(s) 146, 450
BssSI CACGAG 1 cut(s) 516
BssT1I CCWWGG 3 cut(s) 415, 505, 524
Bst2BI CACGAG 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 4 cut(s) 56, 106, 134, 277
Bst6I CTCTTC 1 cut(s) 12
BstACI GRCGYC 2 cut(s) 146, 450
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 3 cut(s) 294, 314, 556
BstDSI CCRYGG 3 cut(s) 102, 415, 505
BstEII GGTNACC 1 cut(s) 18
BstF5I GGATG 1 cut(s) 340
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 5 cut(s) 160, 229, 355, 360, 588
BstMBI GATC 5 cut(s) 157, 226, 352, 357, 585
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 514
BstPI GGTNACC 1 cut(s) 18
BstSCI CCNGG 2 cut(s) 95, 119
BstSLI GKGCMC 1 cut(s) 680
BstV1I GCAGC 1 cut(s) 478
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 2 cut(s) 352, 585
BstYI RGATCY 2 cut(s) 352, 585
BsuRI GGCC 3 cut(s) 420, 437, 647
BtgI CCRYGG 3 cut(s) 102, 415, 505
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 1 cut(s) 512
CaiI CAGNNNCTG 1 cut(s) 643
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 99, 206
CseI GACGC 1 cut(s) 135
Csp6I GTAC 2 cut(s) 244, 562
CviAII CATG 3 cut(s) 416, 506, 511
CviQI GTAC 2 cut(s) 244, 562
DdeI CTNAG 3 cut(s) 294, 314, 556
DpnI GATC 5 cut(s) 159, 228, 354, 359, 587
DpnII GATC 5 cut(s) 157, 226, 352, 357, 585
EaeI YGGCCR 2 cut(s) 418, 645
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 415, 505, 524
Eco147I AGGCCT 1 cut(s) 437
Eco47I GGWCC 2 cut(s) 99, 206
Eco91I GGTNACC 1 cut(s) 18
EcoO65I GGTNACC 1 cut(s) 18
EcoRI GAATTC 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 95
EcoT14I CCWWGG 3 cut(s) 415, 505, 524
ErhI CCWWGG 3 cut(s) 415, 505, 524
FaeI CATG 3 cut(s) 419, 509, 514
FaqI GGGAC 1 cut(s) 506
FatI CATG 3 cut(s) 415, 505, 510
Fnu4HI GCNGC 1 cut(s) 467
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 467
GlaI GCGC 1 cut(s) 118
GluI GCNGC 1 cut(s) 467
HaeIII GGCC 3 cut(s) 420, 437, 647
HapII CCGG 3 cut(s) 23, 120, 194
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 2 cut(s) 146, 450
Hin1II CATG 3 cut(s) 419, 509, 514
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 3 cut(s) 268, 519, 640
HpaII CCGG 3 cut(s) 23, 120, 194
HphI GGTGA 3 cut(s) 12, 151, 208
Hpy166II GTNNAC 2 cut(s) 102, 448
Hpy188III TCNNGA 2 cut(s) 230, 293
Hpy8I GTNNAC 2 cut(s) 102, 448
Hpy99I CGWCG 2 cut(s) 79, 151
HpyAV CCTTC 1 cut(s) 690
HpyCH4III ACNGT 4 cut(s) 56, 106, 134, 277
HpyCH4IV ACGT 2 cut(s) 409, 450
HpyCH4V TGCA 1 cut(s) 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 3 cut(s) 294, 314, 556
HpySE526I ACGT 2 cut(s) 409, 450
Hsp92I GRCGYC 2 cut(s) 146, 450
Hsp92II CATG 3 cut(s) 419, 509, 514
HspAI GCGC 1 cut(s) 117
Kzo9I GATC 5 cut(s) 157, 226, 352, 357, 585
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 1 cut(s) 604
MaeII ACGT 2 cut(s) 409, 450
MaeIII GTNAC 1 cut(s) 18
MalI GATC 5 cut(s) 159, 228, 354, 359, 587
MboI GATC 5 cut(s) 157, 226, 352, 357, 585
MboII GAAGA 4 cut(s) 31, 236, 595, 717
MflI RGATCY 2 cut(s) 352, 585
MhlI GDGCHC 1 cut(s) 680
MlsI TGGCCA 1 cut(s) 420
MluCI AATT 3 cut(s) 163, 382, 619
MluNI TGGCCA 1 cut(s) 420
MlyI GAGTC 1 cut(s) 528
MmeI TCCRAC 3 cut(s) 98, 129, 135
MnlI CCTC 7 cut(s) 135, 273, 277, 329, 565, 636, 726
Mox20I TGGCCA 1 cut(s) 420
MscI TGGCCA 1 cut(s) 420
MseI TTAA 2 cut(s) 126, 167
Msp20I TGGCCA 1 cut(s) 420
MspI CCGG 3 cut(s) 23, 120, 194
MspR9I CCNGG 2 cut(s) 97, 121
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 1 cut(s) 121
NcoI CCATGG 2 cut(s) 415, 505
NdeII GATC 5 cut(s) 157, 226, 352, 357, 585
NlaIII CATG 3 cut(s) 419, 509, 514
NlaIV GGNNCC 4 cut(s) 192, 207, 553, 677
NmeAIII GCCGAG 2 cut(s) 167, 240
NmuCI GTSAC 1 cut(s) 18
NspI RCATGY 1 cut(s) 514
PaeI GCATGC 1 cut(s) 514
PceI AGGCCT 1 cut(s) 437
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 268, 640
PkrI GCNGC 1 cut(s) 468
PleI GAGTC 1 cut(s) 527
PpsI GAGTC 1 cut(s) 527
PsiI TTATAA 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 95
PspEI GGTNACC 1 cut(s) 18
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 4 cut(s) 192, 207, 553, 677
PspPI GGNCC 2 cut(s) 99, 206
PstNI CAGNNNCTG 1 cut(s) 643
PsuI RGATCY 2 cut(s) 352, 585
RsaI GTAC 2 cut(s) 245, 563
RsaNI GTAC 2 cut(s) 244, 562
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 126, 167
SatI GCNGC 1 cut(s) 467
Sau3AI GATC 5 cut(s) 157, 226, 352, 357, 585
Sau96I GGNCC 2 cut(s) 99, 206
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 2 cut(s) 97, 121
SduI GDGCHC 1 cut(s) 680
SetI ASST 8 cut(s) 101, 173, 300, 315, 368, 412, 453, 557
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 2 cut(s) 99, 206
SphI GCATGC 1 cut(s) 514
Sse9I AATT 3 cut(s) 163, 382, 619
SseBI AGGCCT 1 cut(s) 437
SsiI CCGC 1 cut(s) 137
StuI AGGCCT 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 95, 119
StyI CCWWGG 3 cut(s) 415, 505, 524
TaaI ACNGT 4 cut(s) 56, 106, 134, 277
TaiI ACGT 2 cut(s) 412, 453
TaqI TCGA 4 cut(s) 67, 89, 321, 541
TaqII GACCGA 1 cut(s) 6
TasI AATT 3 cut(s) 163, 382, 619
TatI WGTACW 2 cut(s) 243, 561
TfiI GAWTC 2 cut(s) 268, 640
Tru1I TTAA 2 cut(s) 126, 167
Tru9I TTAA 2 cut(s) 126, 167
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 1 cut(s) 466
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 702
TspRI CASTG 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 99, 206
XapI RAATTY 2 cut(s) 163, 619
XceI RCATGY 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 522
ZraI GACGTC 1 cut(s) 451
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.