Rorug01G0297600

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0004386
Physical Location & Seq
Forward (+)
41298642 .. 41315951
17310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0297600.1

Sequence Viewer

Length: 894 bp
ATGGCACCAAAGTTGGGGATTTCACTGGCATTCCTCATTTCAGTAACTCTAGCACTACAAATTGGAGCCAATGCCGCAGTCATTGCGATTTATTGGGGTCAAAATGGGAACGAGGGTACCTTGGAAGAGACCTGTGGCACAGGCAACTATGACTATGTGAACATAGCTTTTCTCCCAACATTCGGCAATGGCCAGACTCCCATGATAAACCTAGCCGGACATTGTGATCCTTCCACTAATGGCTGCACCGGTTTGAGCTCCGACATTAAGTCGTGCCAAGCAAAAGGGGTCAAGGTGATTCTTTCGATTGGAGGAGGAGCAGGGAGCTACTACCTCACATCTAAAGAGGATGCTAGGCAGGTTGCAATCTATTTGTGGAACAATTTCTTGGGAGGAACTTCATCTTCTCGCCCTCTTGGTGACGCTGTTTTGGATGGAATTGATTTTGATATCGAGGGAGGAACAAACCTACATTGGGATGACCTCGCTACATTTCTCTCTGCATACAGCAACCAAGGTAAGAAGGTTTACCTGACCGCAGCGCCCCAGTGCCCTTTCCCTGATGCTTGGGTTGGAGGTGCCCTCAAGACGGGCCTTTTCGACTACGTTTGGGTCCAATTCTACAACAACCCTCCATGCCAATACTCGGCCGATCTCAGCAACCTTGTAAGTGCATGGAAGCAGTGGGTCTCCGATATTCCGGCCACCAAGATATTCCTTGGTCTACCAGCTGCTCCTGAAGCTGCTGGAAGTGGCTTTATTCCTGTCAATGATCTCACTTCTGAAGTGCTTCCAGCAATTAAAGGTTCGTCCAAGTATGGAGGGGTCATGCTTTGGTCCAAGTATTATGATGACCAGACTAAATACAGTGCTTCCATCAAGAACCACCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

297

Amino Acids

31.64

Weight (kDa)

4.87

Isoelectric Point (pI)

29.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 28 - 245 8.8e-22 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 3 cut(s) 4, 116, 578
AccI GTMKAC 1 cut(s) 724
AciI CCGC 2 cut(s) 75, 537
AclWI GGATC 1 cut(s) 221
AcoI YGGCCR 3 cut(s) 190, 648, 702
AcuI CTGAAG 2 cut(s) 759, 804
AfaI GTAC 1 cut(s) 118
AfiI CCNNNNNNNGG 5 cut(s) 14, 182, 475, 589, 646
AgeI ACCGGT 1 cut(s) 248
AhdI GACNNNNNGTC 1 cut(s) 268
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 5 cut(s) 167, 258, 327, 731, 743
AluI AGCT 5 cut(s) 167, 258, 327, 731, 743
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 122, 694
AlwI GGATC 1 cut(s) 221
AoxI GGCC 4 cut(s) 190, 592, 648, 702
ApeKI GCWGC 4 cut(s) 243, 539, 731, 743
AsiGI ACCGGT 1 cut(s) 248
Asp700I GAANNNNTTC 2 cut(s) 383, 789
Asp718I GGTACC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 3 cut(s) 592, 613, 837
AsuHPI GGTGA 2 cut(s) 307, 431
AvaII GGWCC 2 cut(s) 613, 837
BaeGI GKGCMC 2 cut(s) 554, 583
BaeI ACNNNNGTAYC 2 cut(s) 100, 133
BalI TGGCCA 1 cut(s) 192
BanI GGYRCC 3 cut(s) 4, 116, 578
BanII GRGCYC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 4 cut(s) 230, 551, 718, 730
BccI CCATC 2 cut(s) 428, 884
BcoDI GTCTC 2 cut(s) 122, 694
BfaI CTAG 4 cut(s) 50, 212, 354, 892
BfoI RGCGCY 1 cut(s) 545
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 5 cut(s) 75, 244, 540, 732, 744
BlsI GCNGC 5 cut(s) 76, 245, 541, 733, 745
Bme18I GGWCC 2 cut(s) 613, 837
BmeRI GACNNNNNGTC 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 592, 613, 837
BmiI GGNNCC 5 cut(s) 6, 67, 118, 580, 614
BmrI ACTGGG 1 cut(s) 541
BmsI GCATC 2 cut(s) 340, 553
BmuI ACTGGG 1 cut(s) 541
BplI GAGNNNNNCTC 2 cut(s) 567, 599
BpuEI CTTGAG 1 cut(s) 569
BsaI GGTCTC 2 cut(s) 122, 694
BsaJI CCNNGG 3 cut(s) 120, 514, 718
BsaWI WCCGGW 1 cut(s) 248
Bsc4I CCNNNNNNNGG 5 cut(s) 14, 182, 475, 589, 646
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 2 cut(s) 30, 547
Bse3DI GCAATG 2 cut(s) 81, 193
BseDI CCNNGG 3 cut(s) 120, 514, 718
BseGI GGATG 3 cut(s) 355, 439, 484
BseLI CCNNNNNNNGG 5 cut(s) 14, 182, 475, 589, 646
BseMI GCAATG 2 cut(s) 81, 193
BseMII CTCAG 1 cut(s) 670
BseNI ACTGG 2 cut(s) 30, 547
BseRI GAGGAG 2 cut(s) 327, 330
BseSI GKGCMC 2 cut(s) 554, 583
BseX3I CGGCCG 1 cut(s) 648
BseXI GCAGC 4 cut(s) 230, 551, 718, 730
BsgI GTGCAG 1 cut(s) 229
Bsh1285I CGRYCG 1 cut(s) 651
BshFI GGCC 4 cut(s) 192, 594, 650, 704
BshNI GGYRCC 3 cut(s) 4, 116, 578
BshTI ACCGGT 1 cut(s) 248
BsiEI CGRYCG 1 cut(s) 651
BsiHKAI GWGCWC 1 cut(s) 260
BsiSI CCGG 3 cut(s) 216, 249, 701
BslI CCNNNNNNNGG 5 cut(s) 14, 182, 475, 589, 646
BsmAI GTCTC 2 cut(s) 122, 694
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 4 cut(s) 192, 594, 650, 704
Bso31I GGTCTC 2 cut(s) 122, 694
Bsp1286I GDGCHC 3 cut(s) 260, 554, 583
Bsp143I GATC 3 cut(s) 226, 652, 772
BspACI CCGC 2 cut(s) 75, 537
BspANI GGCC 4 cut(s) 192, 594, 650, 704
BspCNI CTCAG 1 cut(s) 669
BspLI GGNNCC 5 cut(s) 6, 67, 118, 580, 614
BspMI ACCTGC 1 cut(s) 349
BspPI GGATC 1 cut(s) 221
BspT107I GGYRCC 3 cut(s) 4, 116, 578
BspTNI GGTCTC 2 cut(s) 122, 694
BsrDI GCAATG 2 cut(s) 81, 193
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 2 cut(s) 30, 547
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 120, 514, 718
BssMI GATC 3 cut(s) 226, 652, 772
BssT1I CCWWGG 3 cut(s) 120, 514, 718
Bst4CI ACNGT 1 cut(s) 869
Bst6I CTCTTC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 83
BstDEI CTNAG 1 cut(s) 656
BstF5I GGATG 3 cut(s) 355, 439, 484
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 3 cut(s) 229, 655, 775
BstMAI GTCTC 2 cut(s) 122, 694
BstMBI GATC 3 cut(s) 226, 652, 772
BstMCI CGRYCG 1 cut(s) 651
BstMWI GCNNNNNNNGC 3 cut(s) 74, 83, 740
BstSLI GKGCMC 2 cut(s) 554, 583
BstV1I GCAGC 4 cut(s) 230, 551, 718, 730
BstZI CGGCCG 1 cut(s) 648
BsuRI GGCC 4 cut(s) 192, 594, 650, 704
BtsCI GGATG 3 cut(s) 355, 439, 484
BtsI GCAGTG 1 cut(s) 689
BtsIMutI CAGTG 4 cut(s) 23, 554, 689, 874
BveI ACCTGC 1 cut(s) 349
CfoI GCGC 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 3 cut(s) 592, 613, 837
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 117
CspAI ACCGGT 1 cut(s) 248
CviAII CATG 4 cut(s) 202, 636, 675, 829
CviQI GTAC 1 cut(s) 117
DdeI CTNAG 1 cut(s) 656
DpnI GATC 3 cut(s) 228, 654, 774
DpnII GATC 3 cut(s) 226, 652, 772
DriI GACNNNNNGTC 1 cut(s) 268
EaeI YGGCCR 3 cut(s) 190, 648, 702
EagI CGGCCG 1 cut(s) 648
Eam1104I CTCTTC 1 cut(s) 120
Eam1105I GACNNNNNGTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 120
Ecl136II GAGCTC 1 cut(s) 258
EclXI CGGCCG 1 cut(s) 648
Eco130I CCWWGG 3 cut(s) 120, 514, 718
Eco24I GRGCYC 1 cut(s) 260
Eco31I GGTCTC 2 cut(s) 122, 694
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 613, 837
Eco52I CGGCCG 1 cut(s) 648
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 2 cut(s) 759, 804
EcoICRI GAGCTC 1 cut(s) 258
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 3 cut(s) 120, 514, 718
EcoT38I GRGCYC 1 cut(s) 260
ErhI CCWWGG 3 cut(s) 120, 514, 718
FaeI CATG 4 cut(s) 205, 639, 678, 832
FatI CATG 4 cut(s) 201, 635, 674, 828
FblI GTMKAC 1 cut(s) 724
Fnu4HI GCNGC 5 cut(s) 75, 244, 540, 732, 744
FokI GGATG 3 cut(s) 362, 446, 491
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 5 cut(s) 75, 244, 540, 732, 744
FspBI CTAG 4 cut(s) 50, 212, 354, 892
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 75, 244, 540, 732, 744
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 4 cut(s) 192, 594, 650, 704
HapII CCGG 3 cut(s) 216, 249, 701
HgaI GACGC 1 cut(s) 431
HhaI GCGC 1 cut(s) 544
Hin1II CATG 4 cut(s) 205, 639, 678, 832
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HinfI GANTC 2 cut(s) 196, 298
HpaII CCGG 3 cut(s) 216, 249, 701
HphI GGTGA 2 cut(s) 307, 431
Hpy166II GTNNAC 3 cut(s) 160, 529, 725
Hpy188I TCNGA 3 cut(s) 262, 694, 784
Hpy188III TCNNGA 3 cut(s) 586, 737, 880
Hpy8I GTNNAC 3 cut(s) 160, 529, 725
HpyAV CCTTC 2 cut(s) 240, 517
HpyCH4III ACNGT 1 cut(s) 869
HpyCH4IV ACGT 1 cut(s) 606
HpyCH4V TGCA 4 cut(s) 246, 365, 503, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 83, 740
HpyF3I CTNAG 1 cut(s) 656
HpySE526I ACGT 1 cut(s) 606
Hsp92II CATG 4 cut(s) 205, 639, 678, 832
HspAI GCGC 1 cut(s) 542
KpnI GGTACC 1 cut(s) 120
Kzo9I GATC 3 cut(s) 226, 652, 772
LmnI GCTCC 5 cut(s) 65, 263, 317, 324, 739
Lsp1109I GCAGC 4 cut(s) 230, 551, 718, 730
LweI GCATC 2 cut(s) 340, 553
MaeI CTAG 4 cut(s) 50, 212, 354, 892
MaeII ACGT 1 cut(s) 606
MaeIII GTNAC 2 cut(s) 43, 419
MalI GATC 3 cut(s) 228, 654, 774
MboI GATC 3 cut(s) 226, 652, 772
MboII GAAGA 2 cut(s) 137, 396
MhlI GDGCHC 3 cut(s) 260, 554, 583
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 5 cut(s) 60, 382, 438, 617, 798
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 2 cut(s) 285, 553
Mox20I TGGCCA 1 cut(s) 192
MroXI GAANNNNTTC 2 cut(s) 383, 789
MscI TGGCCA 1 cut(s) 192
MseI TTAA 2 cut(s) 267, 801
MslI CAYNNNNRTG 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 192
MspA1I CMGCKG 1 cut(s) 731
MspI CCGG 3 cut(s) 216, 249, 701
Mva1269I GAATGC 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 74, 83, 740
NdeII GATC 3 cut(s) 226, 652, 772
NlaIII CATG 4 cut(s) 205, 639, 678, 832
NlaIV GGNNCC 5 cut(s) 6, 67, 118, 580, 614
NmeAIII GCCGAG 1 cut(s) 626
NmuCI GTSAC 1 cut(s) 419
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 2 cut(s) 383, 789
PfeI GAWTC 1 cut(s) 298
PinAI ACCGGT 1 cut(s) 248
PkrI GCNGC 5 cut(s) 76, 245, 541, 733, 745
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 260
PspN4I GGNNCC 5 cut(s) 6, 67, 118, 580, 614
PspPI GGNCC 3 cut(s) 592, 613, 837
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 1 cut(s) 118
RsaNI GTAC 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 477
SacI GAGCTC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 267, 801
SatI GCNGC 5 cut(s) 75, 244, 540, 732, 744
Sau3AI GATC 3 cut(s) 226, 652, 772
Sau96I GGNCC 3 cut(s) 592, 613, 837
SchI GAGTC 1 cut(s) 190
SduI GDGCHC 3 cut(s) 260, 554, 583
SfaNI GCATC 2 cut(s) 340, 553
SinI GGWCC 2 cut(s) 613, 837
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 5 cut(s) 60, 382, 438, 617, 798
SsiI CCGC 2 cut(s) 75, 537
SspMI CTAG 4 cut(s) 50, 212, 354, 892
SstI GAGCTC 1 cut(s) 260
StyI CCWWGG 3 cut(s) 120, 514, 718
TaaI ACNGT 1 cut(s) 869
TaiI ACGT 1 cut(s) 609
TaqI TCGA 3 cut(s) 305, 453, 600
TasI AATT 5 cut(s) 60, 382, 438, 617, 798
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 2 cut(s) 267, 801
Tru9I TTAA 2 cut(s) 267, 801
TscAI CASTG 4 cut(s) 30, 554, 689, 874
TseFI GTSAC 1 cut(s) 419
TseI GCWGC 4 cut(s) 243, 539, 731, 743
Tsp45I GTSAC 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 390
TspRI CASTG 4 cut(s) 30, 554, 689, 874
VpaK11BI GGWCC 2 cut(s) 613, 837
XmiI GTMKAC 1 cut(s) 724
XmnI GAANNNNTTC 2 cut(s) 383, 789
XspI CTAG 4 cut(s) 50, 212, 354, 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.