Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0004386

Physical Location & Seq

Genomic Coordinates Reverse (-)

17239461 .. 17242762

Gene Structure 3302 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1108700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGGGGATTCTGCATGAGGATGTGGTGCTCATCAAGCAGGCGGAGAAAGAAGGGGACCCAAGTGTAATTACAATCAATTGTCCGGACAAGACTGGCTTGGGTTGTGACTTGTGTCGCATCATTCTCTTCTTTGGTCTTAGCATTGTCCGGGGAGATGTATCGACGGATGGGAAATGGTGCTACTTAGTGTTTTGGGTGATAGGGAGTCCAGAGACAAGGTGGGGTTTGTTGAAGAAGAGGCTAGTGGGGACCTGCCCTTCTTGTTCTTCGGCTTCTGGGATTTCGTTCTACCGCTCCGAATTGCAGCCCCCAAAGCCTCCTGACGTGTTCCTTCTCAAGTTCTGTTGCTACAATCGACGAGGACTTTTACATGATGTAACGGGTGTCCTCTGTGAACTTGAGCTGACGATAAAGAAAGTTAAGGTATCCACAACTCCAGATGAGAAAGTGATGGACCTGTTTTTCGTCACAGACACCAGGGAACTTATGCATACAAATAAGAGAAGGGAGGAGGTATATGACTACTTAAAAGAAGTCATGGGGGACGCCATGATAAGTTGTGAAATTGAAACGGCTGGCCCTGAGATTACTGCATGTACGCAGGCGTCTTCATTTCTTCCAACTGCAATTACAGAAGACATGTTTGATTTGGAAATGCCCAAAGAACTCCGAAGTGGATCACTCACTTCCAAAAGTGTTTCTGTGACCATGGATAACACGCTCAGTCCTGGTCACACGCTGGTCCAAATCATTTGCATAGACCACAAAGGTCTTCTATATGATATAATGAGAACGCTGAAGGATTACAATATTCAGATCTCCTATGGGCGCTTCTCTATAAAACAAAAAAGGCACTGTGAGATTGACTTGTTTGTCATGCAAGCTGATGGAAAGAAGATAGTTGACTCCAGCAAGCAGACTGCATTGACATCCCGTTTGCAGCTGGAACTGATTCGTCCTCTCAGAGTAGCTATTGTTAACCGAGGTCCGGACACAGAGCTCCTTGTTGCAAACCCTGTGGAACTATCTGGCAAGGGCCGGCCTCTTGTTTTTTATGATATCACCCTTGCTTTCAAGATGCTGGACATTGGTGTCTTTTCGGCTAAGATTGGACGGCATCTGATTGGAGATAGGGAATGGGAAGTTTATAGAGTCTTAATTGACGAAGGAGTTGGCTTGTCAGTTCCAAGGAACACGATCGAGGAACAGGTTTGGAAGATGCTGATGGGTTGGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ACT

Protein Analysis

413

Amino Acids

46.42

Weight (kDa)

5.91

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACR10_N	PF24914	11 - 86	2.9e-39	ACT domain-containing protein ACR10, N-terminal
ACT_ACR9_3rd	PF24931	234 - 331	5.9e-49	ACR9-like, ACT-like domain
ACT_ACR9_C	PF24926	332 - 410	2e-37	ACT domain-containing protein ACR9, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004386)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36840
fragaria_vesca	FvH4_6g16600 FvH4_6g16600 FvH4_7g18540
malus_domestica	MD01G1101800.v1.1 MD04G1090700.v1.1 MD12G1108700.v1.1
prunus_persica	Prupe.2G207000_v2.0.a1 Prupe.7G127300_v2.0.a1
pyrus_communis	pycom01g12840 pycom04g08590 pycom12g10900
rosa_chinensis	RchiOBHm_Chr1g0362321 RchiOBHm_Chr2g0171731 RchiOBHm_Chr3g0470541
rosa_laevigata	RLG00000024260 RLG00000027651
rosa_multiflora	Rmu_sc0002115.1_g000012 Rmu_sc0020251.1_g000001
rosa_roxburghii	Rroxscaffold_4G00294250 Rroxscaffold_6G00410740
rosa_rugosa	Rorug01G0297600 Rorug01G0297700 Rorug03G0109900
rosa_samantha	Rh1AG306300 Rh1DG300000 Rh3AG160000 Rh3BG184600 Rh3CG175100 Rh3DG190100
rosa_wichuraiana	Rw1G027190 Rw3G015140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	872, 1091
Acc36I	ACCTGC	1 cut(s)	260
AccBSI	CCGCTC	1 cut(s)	294
AccIII	TCCGGA	2 cut(s)	82, 988
AciI	CCGC	2 cut(s)	41, 292
AclWI	GGATC	1 cut(s)	685
AcuI	CTGAAG	1 cut(s)	818
AcyI	GRCGYC	2 cut(s)	546, 605
AfaI	GTAC	1 cut(s)	598
AfiI	CCNNNNNNNGG	1 cut(s)	988
AflIII	ACRYGT	2 cut(s)	324, 639
AgsI	TTSAA	3 cut(s)	232, 569, 1075
AjiI	CACGTC	1 cut(s)	325
AjnI	CCWGG	2 cut(s)	476, 727
AluBI	AGCT	5 cut(s)	403, 884, 943, 971, 1000
AluI	AGCT	5 cut(s)	403, 884, 943, 971, 1000
Alw21I	GWGCWC	2 cut(s)	30, 1002
Alw26I	GTCTC	1 cut(s)	206
AlwI	GGATC	1 cut(s)	685
Aor13HI	TCCGGA	2 cut(s)	82, 988
AoxI	GGCC	3 cut(s)	577, 1036, 1040
ApeKI	GCWGC	2 cut(s)	304, 940
ArsI	GACNNNNNNTTYG	4 cut(s)	446, 478, 919, 951
Asp700I	GAANNNNTTC	1 cut(s)	951
AspLEI	GCGC	1 cut(s)	831
AspS9I	GGNCC	7 cut(s)	55, 249, 454, 578, 742, 986, 1036
AsuC2I	CCSGG	1 cut(s)	149
AsuHPI	GGTGA	2 cut(s)	208, 1054
AvaII	GGWCC	5 cut(s)	55, 249, 454, 742, 986
BanII	GRGCYC	1 cut(s)	1002
BbsI	GAAGAC	3 cut(s)	600, 642, 764
Bbv12I	GWGCWC	2 cut(s)	30, 1002
BbvI	GCAGC	2 cut(s)	316, 952
BccI	CCATC	4 cut(s)	161, 445, 881, 1219
BceAI	ACGGC	2 cut(s)	588, 1130
BciT130I	CCWGG	2 cut(s)	478, 729
BciVI	GTATCC	1 cut(s)	436
BcnI	CCSGG	1 cut(s)	149
BcoDI	GTCTC	1 cut(s)	206
BfaI	CTAG	1 cut(s)	242
BfoI	RGCGCY	1 cut(s)	832
BfuAI	ACCTGC	1 cut(s)	260
BfuI	GTATCC	1 cut(s)	436
BglII	AGATCT	1 cut(s)	816
BisI	GCNGC	2 cut(s)	305, 941
BlsI	GCNGC	2 cut(s)	306, 942
Bme1390I	CCNGG	3 cut(s)	149, 478, 729
Bme18I	GGWCC	5 cut(s)	55, 249, 454, 742, 986
BmgBI	CACGTC	1 cut(s)	325
BmgT120I	GGNCC	7 cut(s)	55, 249, 454, 578, 742, 986, 1036
BmiI	GGNNCC	3 cut(s)	56, 57, 250
BmrFI	CCNGG	3 cut(s)	149, 478, 729
BmsI	GCATC	4 cut(s)	126, 1068, 1126, 1209
BoxI	GACNNNNGTC	1 cut(s)	111
BpiI	GAAGAC	3 cut(s)	600, 642, 764
BpmI	CTGGAG	2 cut(s)	420, 892
BpuEI	CTTGAG	2 cut(s)	320, 419
BpuMI	CCSGG	1 cut(s)	149
BsaHI	GRCGYC	2 cut(s)	546, 605
BsaJI	CCNNGG	5 cut(s)	148, 477, 708, 982, 1187
BsaWI	WCCGGW	2 cut(s)	82, 988
Bsc4I	CCNNNNNNNGG	1 cut(s)	988
Bse118I	RCCGGY	1 cut(s)	1038
Bse1I	ACTGG	1 cut(s)	97
BseAI	TCCGGA	2 cut(s)	82, 988
BseBI	CCWGG	2 cut(s)	478, 729
BseDI	CCNNGG	5 cut(s)	148, 477, 708, 982, 1187
BseGI	GGATG	3 cut(s)	25, 172, 929
BseLI	CCNNNNNNNGG	1 cut(s)	988
BseMII	CTCAG	3 cut(s)	573, 736, 976
BseNI	ACTGG	1 cut(s)	97
BseRI	GAGGAG	1 cut(s)	524
BseXI	GCAGC	2 cut(s)	316, 952
Bsh1285I	CGRYCG	1 cut(s)	1200
BshFI	GGCC	3 cut(s)	579, 1038, 1042
BsiEI	CGRYCG	1 cut(s)	1200
BsiHKAI	GWGCWC	2 cut(s)	30, 1002
BsiSI	CCGG	4 cut(s)	83, 148, 989, 1039
BslFI	GGGAC	3 cut(s)	68, 262, 557
BslI	CCNNNNNNNGG	1 cut(s)	988
BsmAI	GTCTC	1 cut(s)	206
BsmFI	GGGAC	3 cut(s)	68, 262, 557
BsnI	GGCC	3 cut(s)	579, 1038, 1042
Bsp1286I	GDGCHC	2 cut(s)	30, 1002
Bsp13I	TCCGGA	2 cut(s)	82, 988
Bsp143I	GATC	3 cut(s)	677, 816, 1197
Bsp19I	CCATGG	1 cut(s)	708
BspACI	CCGC	2 cut(s)	41, 292
BspANI	GGCC	3 cut(s)	579, 1038, 1042
BspCNI	CTCAG	3 cut(s)	574, 735, 975
BspEI	TCCGGA	2 cut(s)	82, 988
BspLI	GGNNCC	3 cut(s)	56, 57, 250
BspMI	ACCTGC	1 cut(s)	260
BspPI	GGATC	1 cut(s)	685
BsrBI	CCGCTC	1 cut(s)	294
BsrFI	RCCGGY	1 cut(s)	1038
BsrI	ACTGG	1 cut(s)	97
BssAI	RCCGGY	1 cut(s)	1038
BssECI	CCNNGG	5 cut(s)	148, 477, 708, 982, 1187
BssMI	GATC	3 cut(s)	677, 816, 1197
BssNI	GRCGYC	2 cut(s)	546, 605
BssT1I	CCWWGG	2 cut(s)	708, 1187
Bst2UI	CCWGG	2 cut(s)	478, 729
Bst4CI	ACNGT	1 cut(s)	857
Bst6I	CTCTTC	2 cut(s)	131, 230
BstACI	GRCGYC	2 cut(s)	546, 605
BstC8I	GCNNGC	6 cut(s)	39, 577, 603, 882, 914, 1040
BstDEI	CTNAG	6 cut(s)	137, 184, 582, 722, 962, 1104
BstDSI	CCRYGG	1 cut(s)	708
BstF5I	GGATG	3 cut(s)	25, 172, 929
BstH2I	RGCGCY	1 cut(s)	832
BstHHI	GCGC	1 cut(s)	831
BstKTI	GATC	3 cut(s)	680, 819, 1200
BstMAI	GTCTC	1 cut(s)	206
BstMBI	GATC	3 cut(s)	677, 816, 1197
BstMCI	CGRYCG	1 cut(s)	1200
BstMWI	GCNNNNNNNGC	2 cut(s)	34, 313
BstNI	CCWGG	2 cut(s)	478, 729
BstNSI	RCATGY	2 cut(s)	597, 643
BstPAI	GACNNNNGTC	1 cut(s)	111
BstSCI	CCNGG	3 cut(s)	147, 476, 727
BstV1I	GCAGC	2 cut(s)	316, 952
BstV2I	GAAGAC	3 cut(s)	600, 642, 764
BstX2I	RGATCY	1 cut(s)	816
BstYI	RGATCY	1 cut(s)	816
BsuI	GTATCC	1 cut(s)	436
BsuRI	GGCC	3 cut(s)	579, 1038, 1042
BtgI	CCRYGG	1 cut(s)	708
BtrI	CACGTC	1 cut(s)	325
BtsCI	GGATG	3 cut(s)	25, 172, 929
BtsIMutI	CAGTG	1 cut(s)	853
BveI	ACCTGC	1 cut(s)	260
Cac8I	GCNNGC	6 cut(s)	39, 577, 603, 882, 914, 1040
CfoI	GCGC	1 cut(s)	831
Cfr10I	RCCGGY	1 cut(s)	1038
Cfr13I	GGNCC	7 cut(s)	55, 249, 454, 578, 742, 986, 1036
CseI	GACGC	2 cut(s)	554, 594
Csp6I	GTAC	1 cut(s)	597
CspCI	CAANNNNNGTGG	2 cut(s)	999, 1034
CviAII	CATG	8 cut(s)	14, 371, 538, 550, 594, 640, 709, 877
CviQI	GTAC	1 cut(s)	597
DdeI	CTNAG	6 cut(s)	137, 184, 582, 722, 962, 1104
DpnI	GATC	3 cut(s)	679, 818, 1199
DpnII	GATC	3 cut(s)	677, 816, 1197
DrdI	GACNNNNNNGTC	2 cut(s)	872, 1091
DseDI	GACNNNNNNGTC	2 cut(s)	872, 1091
Eam1104I	CTCTTC	2 cut(s)	131, 230
EarI	CTCTTC	2 cut(s)	131, 230
EciI	GGCGGA	1 cut(s)	56
Ecl136II	GAGCTC	1 cut(s)	1000
Eco130I	CCWWGG	2 cut(s)	708, 1187
Eco24I	GRGCYC	1 cut(s)	1002
Eco32I	GATATC	1 cut(s)	1060
Eco47I	GGWCC	5 cut(s)	55, 249, 454, 742, 986
Eco53kI	GAGCTC	1 cut(s)	1000
Eco57I	CTGAAG	1 cut(s)	818
EcoICRI	GAGCTC	1 cut(s)	1000
EcoO109I	RGGNCCY	2 cut(s)	55, 249
EcoRII	CCWGG	2 cut(s)	476, 727
EcoRV	GATATC	1 cut(s)	1060
EcoT14I	CCWWGG	2 cut(s)	708, 1187
EcoT22I	ATGCAT	1 cut(s)	492
EcoT38I	GRGCYC	1 cut(s)	1002
ErhI	CCWWGG	2 cut(s)	708, 1187
FaeI	CATG	8 cut(s)	17, 374, 541, 553, 597, 643, 712, 880
FalI	AAGNNNNNCTT	2 cut(s)	80, 112
FaqI	GGGAC	3 cut(s)	68, 262, 557
FatI	CATG	8 cut(s)	13, 370, 537, 549, 593, 639, 708, 876
Fnu4HI	GCNGC	2 cut(s)	305, 941
FokI	GGATG	3 cut(s)	32, 179, 916
FriOI	GRGCYC	1 cut(s)	1002
FseI	GGCCGGCC	1 cut(s)	1042
Fsp4HI	GCNGC	2 cut(s)	305, 941
FspBI	CTAG	1 cut(s)	242
GlaI	GCGC	1 cut(s)	830
GluI	GCNGC	2 cut(s)	305, 941
GsuI	CTGGAG	2 cut(s)	420, 892
HaeII	RGCGCY	1 cut(s)	832
HaeIII	GGCC	3 cut(s)	579, 1038, 1042
HapII	CCGG	4 cut(s)	83, 148, 989, 1039
HgaI	GACGC	2 cut(s)	554, 594
HhaI	GCGC	1 cut(s)	831
Hin1I	GRCGYC	2 cut(s)	546, 605
Hin1II	CATG	8 cut(s)	17, 374, 541, 553, 597, 643, 712, 880
Hin6I	GCGC	1 cut(s)	829
HinP1I	GCGC	1 cut(s)	829
HincII	GTYRAC	2 cut(s)	904, 979
HindII	GTYRAC	2 cut(s)	904, 979
HinfI	GANTC	5 cut(s)	7, 205, 905, 952, 1152
HpaI	GTTAAC	1 cut(s)	979
HpaII	CCGG	4 cut(s)	83, 148, 989, 1039
HphI	GGTGA	2 cut(s)	208, 1054
Hpy166II	GTNNAC	3 cut(s)	395, 904, 979
Hpy188I	TCNGA	5 cut(s)	298, 671, 816, 965, 1122
Hpy188III	TCNNGA	6 cut(s)	83, 209, 320, 437, 989, 1075
Hpy8I	GTNNAC	3 cut(s)	395, 904, 979
Hpy99I	CGWCG	2 cut(s)	166, 360
HpyAV	CCTTC	6 cut(s)	44, 267, 341, 498, 793, 1160
HpyCH4III	ACNGT	1 cut(s)	857
HpyCH4IV	ACGT	1 cut(s)	324
HpyF10VI	GCNNNNNNNGC	2 cut(s)	34, 313
HpyF3I	CTNAG	6 cut(s)	137, 184, 582, 722, 962, 1104
HpySE526I	ACGT	1 cut(s)	324
Hsp92I	GRCGYC	2 cut(s)	546, 605
Hsp92II	CATG	8 cut(s)	17, 374, 541, 553, 597, 643, 712, 880
HspAI	GCGC	1 cut(s)	829
KflI	GGGWCCC	1 cut(s)	55
Kpn2I	TCCGGA	2 cut(s)	82, 988
KroI	GCCGGC	1 cut(s)	1038
KroNI	GCCGGC	1 cut(s)	1040
KspAI	GTTAAC	1 cut(s)	979
Kzo9I	GATC	3 cut(s)	677, 816, 1197
LmnI	GCTCC	2 cut(s)	299, 1005
Lsp1109I	GCAGC	2 cut(s)	316, 952
LweI	GCATC	4 cut(s)	126, 1068, 1126, 1209
MaeI	CTAG	1 cut(s)	242
MaeII	ACGT	1 cut(s)	324
MaeIII	GTNAC	5 cut(s)	104, 376, 466, 703, 731
MalI	GATC	3 cut(s)	679, 818, 1199
MbiI	CCGCTC	1 cut(s)	294
MboI	GATC	3 cut(s)	677, 816, 1197
MfeI	CAATTG	1 cut(s)	76
MflI	RGATCY	1 cut(s)	816
MhlI	GDGCHC	2 cut(s)	30, 1002
MluCI	AATT	6 cut(s)	66, 76, 299, 564, 627, 1158
MlyI	GAGTC	3 cut(s)	214, 899, 1161
MmeI	TCCRAC	1 cut(s)	644
Mph1103I	ATGCAT	1 cut(s)	492
MroI	TCCGGA	2 cut(s)	82, 988
MroNI	GCCGGC	1 cut(s)	1038
MroXI	GAANNNNTTC	1 cut(s)	951
MseI	TTAA	4 cut(s)	420, 527, 978, 1157
MslI	CAYNNNNRTG	1 cut(s)	18
MspA1I	CMGCKG	1 cut(s)	943
MspI	CCGG	4 cut(s)	83, 148, 989, 1039
MspR9I	CCNGG	3 cut(s)	149, 478, 729
MunI	CAATTG	1 cut(s)	76
MvaI	CCWGG	2 cut(s)	478, 729
MwoI	GCNNNNNNNGC	2 cut(s)	34, 313
NaeI	GCCGGC	1 cut(s)	1040
NciI	CCSGG	1 cut(s)	149
NcoI	CCATGG	1 cut(s)	708
NdeII	GATC	3 cut(s)	677, 816, 1197
NgoMIV	GCCGGC	1 cut(s)	1038
NlaIII	CATG	8 cut(s)	17, 374, 541, 553, 597, 643, 712, 880
NlaIV	GGNNCC	3 cut(s)	56, 57, 250
NmuCI	GTSAC	4 cut(s)	104, 466, 703, 731
NsiI	ATGCAT	1 cut(s)	492
NspI	RCATGY	2 cut(s)	597, 643
PciI	ACATGT	1 cut(s)	639
PdiI	GCCGGC	1 cut(s)	1040
PdmI	GAANNNNTTC	1 cut(s)	951
PfeI	GAWTC	2 cut(s)	7, 952
PkrI	GCNGC	2 cut(s)	306, 942
Ple19I	CGATCG	1 cut(s)	1200
PleI	GAGTC	3 cut(s)	213, 899, 1160
PpsI	GAGTC	3 cut(s)	213, 899, 1160
PpuMI	RGGWCCY	2 cut(s)	55, 249
PscI	ACATGT	1 cut(s)	639
PshAI	GACNNNNGTC	1 cut(s)	111
Psp124BI	GAGCTC	1 cut(s)	1002
Psp5II	RGGWCCY	2 cut(s)	55, 249
Psp6I	CCWGG	2 cut(s)	476, 727
PspGI	CCWGG	2 cut(s)	476, 727
PspN4I	GGNNCC	3 cut(s)	56, 57, 250
PspPI	GGNCC	7 cut(s)	55, 249, 454, 578, 742, 986, 1036
PspPPI	RGGWCCY	2 cut(s)	55, 249
PsuI	RGATCY	1 cut(s)	816
PvuI	CGATCG	1 cut(s)	1200
PvuII	CAGCTG	1 cut(s)	943
RigI	GGCCGGCC	1 cut(s)	1042
RsaI	GTAC	1 cut(s)	598
RsaNI	GTAC	1 cut(s)	597
RseI	CAYNNNNRTG	1 cut(s)	18
SacI	GAGCTC	1 cut(s)	1002
SaqAI	TTAA	4 cut(s)	420, 527, 978, 1157
SatI	GCNGC	2 cut(s)	305, 941
Sau3AI	GATC	3 cut(s)	677, 816, 1197
Sau96I	GGNCC	7 cut(s)	55, 249, 454, 578, 742, 986, 1036
SchI	GAGTC	3 cut(s)	214, 899, 1161
ScrFI	CCNGG	3 cut(s)	149, 478, 729
SduI	GDGCHC	2 cut(s)	30, 1002
SfaNI	GCATC	4 cut(s)	126, 1068, 1126, 1209
SinI	GGWCC	5 cut(s)	55, 249, 454, 742, 986
SmiMI	CAYNNNNRTG	1 cut(s)	18
SmlI	CTYRAG	2 cut(s)	335, 398
SmoI	CTYRAG	2 cut(s)	335, 398
Sse9I	AATT	6 cut(s)	66, 76, 299, 564, 627, 1158
SsiI	CCGC	2 cut(s)	41, 292
SspI	AATATT	1 cut(s)	811
SspMI	CTAG	1 cut(s)	242
SstI	GAGCTC	1 cut(s)	1002
StyD4I	CCNGG	3 cut(s)	147, 476, 727
StyI	CCWWGG	2 cut(s)	708, 1187
TaaI	ACNGT	1 cut(s)	857
TaiI	ACGT	1 cut(s)	327
TaqI	TCGA	3 cut(s)	161, 355, 1200
TasI	AATT	6 cut(s)	66, 76, 299, 564, 627, 1158
TfiI	GAWTC	2 cut(s)	7, 952
Tru1I	TTAA	4 cut(s)	420, 527, 978, 1157
Tru9I	TTAA	4 cut(s)	420, 527, 978, 1157
TscAI	CASTG	1 cut(s)	860
TseFI	GTSAC	4 cut(s)	104, 466, 703, 731
TseI	GCWGC	2 cut(s)	304, 940
Tsp45I	GTSAC	4 cut(s)	104, 466, 703, 731
TspDTI	ATGAA	1 cut(s)	600
TspGWI	ACGGA	1 cut(s)	179
TspRI	CASTG	1 cut(s)	860
VpaK11BI	GGWCC	5 cut(s)	55, 249, 454, 742, 986
XceI	RCATGY	2 cut(s)	597, 643
XcmI	CCANNNNNNNNNTGG	1 cut(s)	216
XmnI	GAANNNNTTC	1 cut(s)	951
XspI	CTAG	1 cut(s)	242
Zsp2I	ATGCAT	1 cut(s)	492

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.