RLG00000027651

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004386
Physical Location & Seq
Forward (+)
13556587 .. 13559870
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027651

Sequence Viewer

Length: 1242 bp
ATGGGCATTTTGCATGAGGACATGGTGGTAATTAGCCAGGCAGAGAAGGCTGGAGATCCTACCGTGATCACTGTGAATTGCCCTGACAAAACTGGGCTAGGCTGTGATCTGTGCAGGGTCATATTGCTCTTTGGGATAAGCATTTGCAGAGGAGATGTTTCGACGGATGGAAAGTGGTGCTACGTAGTGTTTTGGGTGGTGGGAAAACCGGAAACAAGGTGGAATTTGTTGACAAAGAGATTGTTAGAAGTTTGTCCCTCACATTTTACAACTTCTGGAATATACTATTACCGGCCGGAAAACCAGCAGCCTAAGCCATTAGATGTGTTCCTTTTGAAATTCTGGTGCTCTTATGAACAGGAAGGCCTATTGCATGATGTCACTAAGGTTCTTTGTGAGCTTGAGCTCACAATAGAGAGAGTGAAGGTGTCTACTACCCCAGATGGGAAAGTGATGGACCTTTTTTTTGTTACAGATACCAGAGAACTGCTGCATACAAAAAGAAGACAAGATGAAACAATTAATTGCCTGAAAGCTGTCTTGGGGAATGTCTTGTTGGGTTGTGAAATTGAATTGGCTGAGTCAAAAGTTACCGCATGTTCACAGGGTTCTCCTTATCTTCCTTCTGTAATCACTGAAGAAATGTTAAGTTCAGAGTGGACCAATGGACGCCTAAAAAGACCTGTTGCCTCCATTCCCATTTCAATAACAGTGGACAATAACCTCAGCCCCTCTCACACACTAGTTCAAATATTCTGTCCGGATCATAAAGGTCTAGTGTATGACATCATGAGAACACTAAAGGATTATGACATTCAGATTTCTTATGGACGATTCTATGAAAACACAAAAGGGAATTGTGAGGTGGACTTGTTCATAATGCAAGCAGATGGCAAGAAGATTGTTGACCCCAATAAGCAAGATGCCTTATGTTCTCGCTTGAGAACGGAGCTTTTGTGTCCCCTTCGAGTGGCTGTGGTGAACCGTGGCCCTGACACTGAGCTGTTGGTAGCTAACCCTGTTGAGTTGTCTGGCAAGGGTCGGCCTCTTGTCTTTTATGATATTTCTCTTGCCCTCAAAGATCTGAGCACGCGCATCTTTTCGGCGGAGATAGTGAGACACATGATCCGAGATCGGGAGTGGGAGGTCTACAGATTCCTGCTAGATGAAGGAGTCTGTCCTTCAGCGCCAAAGAACAAAATTGAGGAAGGTGTAAGAAATATACTGATGGGTTGGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

414

Amino Acids

46.78

Weight (kDa)

5.57

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 11 - 86 6.1e-39 ACT domain-containing protein ACR10, N-terminal
ACT_ACR9_3rd PF24931 235 - 332 3.5e-50 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 333 - 411 7.5e-37 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 431, 1149
AccII CGCG 1 cut(s) 1093
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 2 cut(s) 594, 1106
AclWI GGATC 3 cut(s) 50, 771, 1120
AcoI YGGCCR 1 cut(s) 293
AcsI RAATTY 2 cut(s) 223, 338
AcuI CTGAAG 2 cut(s) 657, 1167
AcyI GRCGYC 1 cut(s) 670
AfiI CCNNNNNNNGG 3 cut(s) 444, 970, 1135
AgsI TTSAA 4 cut(s) 337, 572, 705, 749
AhlI ACTAGT 1 cut(s) 742
AjnI CCWGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 4 cut(s) 524, 539, 556, 571
AluBI AGCT 6 cut(s) 400, 406, 536, 952, 1003, 1013
AluI AGCT 6 cut(s) 400, 406, 536, 952, 1003, 1013
Alw21I GWGCWC 3 cut(s) 350, 408, 1091
Alw26I GTCTC 1 cut(s) 1111
AlwI GGATC 3 cut(s) 50, 771, 1120
Aor13HI TCCGGA 1 cut(s) 760
AoxI GGCC 4 cut(s) 293, 364, 988, 1043
ApeKI GCWGC 2 cut(s) 307, 490
ApoI RAATTY 2 cut(s) 223, 338
ArsI GACNNNNNNTTYG 4 cut(s) 449, 481, 742, 774
AseI ATTAAT 1 cut(s) 522
AspLEI GCGC 2 cut(s) 1095, 1189
AspS9I GGNCC 3 cut(s) 457, 660, 989
AsuHPI GGTGA 1 cut(s) 991
AvaII GGWCC 2 cut(s) 457, 660
BanII GRGCYC 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 511
Bbv12I GWGCWC 3 cut(s) 350, 408, 1091
BbvCI CCTCAGC 1 cut(s) 725
BbvI GCAGC 2 cut(s) 319, 477
BccI CCATC 5 cut(s) 161, 437, 448, 884, 1222
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 1111
BcuI ACTAGT 1 cut(s) 742
BfaI CTAG 4 cut(s) 98, 743, 776, 1163
BfmI CTRYAG 1 cut(s) 1150
BfoI RGCGCY 1 cut(s) 1190
BglII AGATCT 1 cut(s) 1081
BisI GCNGC 2 cut(s) 308, 491
BlsI GCNGC 2 cut(s) 309, 492
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 2 cut(s) 457, 660
BmgT120I GGNCC 3 cut(s) 457, 660, 989
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 102
BmsI GCATC 2 cut(s) 913, 1104
BmuI ACTGGG 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 511
BpmI CTGGAG 1 cut(s) 72
Bpu10I CCTNAGC 2 cut(s) 312, 725
BpuEI CTTGAG 2 cut(s) 422, 961
BsaAI YACGTR 1 cut(s) 184
BsaHI GRCGYC 1 cut(s) 670
BsaJI CCNNGG 1 cut(s) 985
BsaWI WCCGGW 2 cut(s) 208, 760
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 3 cut(s) 444, 970, 1135
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 1 cut(s) 97
BseAI TCCGGA 1 cut(s) 760
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 985
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 3 cut(s) 444, 970, 1135
BseMII CTCAG 4 cut(s) 570, 739, 990, 1076
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 293
BseXI GCAGC 2 cut(s) 319, 477
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 1093
Bsh1285I CGRYCG 1 cut(s) 296
BshFI GGCC 4 cut(s) 295, 366, 990, 1045
BsiEI CGRYCG 1 cut(s) 296
BsiHKAI GWGCWC 3 cut(s) 350, 408, 1091
BsiSI CCGG 4 cut(s) 209, 292, 296, 761
BslFI GGGAC 2 cut(s) 240, 945
BslI CCNNNNNNNGG 3 cut(s) 444, 970, 1135
BsmAI GTCTC 1 cut(s) 1111
BsmFI GGGAC 2 cut(s) 240, 945
BsnI GGCC 4 cut(s) 295, 366, 990, 1045
Bsp1286I GDGCHC 3 cut(s) 350, 408, 1091
Bsp13I TCCGGA 1 cut(s) 760
Bsp143I GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
BspACI CCGC 2 cut(s) 594, 1106
BspANI GGCC 4 cut(s) 295, 366, 990, 1045
BspCNI CTCAG 4 cut(s) 571, 738, 991, 1077
BspEI TCCGGA 1 cut(s) 760
BspFNI CGCG 1 cut(s) 1093
BspHI TCATGA 1 cut(s) 789
BspPI GGATC 3 cut(s) 50, 771, 1120
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 1 cut(s) 97
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 1 cut(s) 985
BssMI GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
BssNI GRCGYC 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 4 cut(s) 64, 73, 712, 986
BstACI GRCGYC 1 cut(s) 670
BstBAI YACGTR 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 885, 1091
BstDEI CTNAG 6 cut(s) 312, 384, 579, 725, 999, 1085
BstDSI CCRYGG 1 cut(s) 985
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 1 cut(s) 1093
BstH2I RGCGCY 1 cut(s) 1190
BstHHI GCGC 2 cut(s) 1095, 1189
BstKTI GATC 7 cut(s) 58, 69, 109, 766, 1084, 1128, 1135
BstMAI GTCTC 1 cut(s) 1111
BstMBI GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
BstMCI CGRYCG 1 cut(s) 296
BstMWI GCNNNNNNNGC 2 cut(s) 47, 313
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 600
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 1150
BstSNI TACGTA 1 cut(s) 184
BstUI CGCG 1 cut(s) 1093
BstV1I GCAGC 2 cut(s) 319, 477
BstV2I GAAGAC 1 cut(s) 511
BstX2I RGATCY 2 cut(s) 55, 1081
BstYI RGATCY 2 cut(s) 55, 1081
BstZI CGGCCG 1 cut(s) 293
BsuRI GGCC 4 cut(s) 295, 366, 990, 1045
BtgI CCRYGG 1 cut(s) 985
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 4 cut(s) 69, 633, 717, 996
Cac8I GCNNGC 2 cut(s) 885, 1091
CciI TCATGA 1 cut(s) 789
CfoI GCGC 2 cut(s) 1095, 1189
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 3 cut(s) 457, 660, 989
CseI GACGC 1 cut(s) 678
CspCI CAANNNNNGTGG 2 cut(s) 693, 728
CviAII CATG 6 cut(s) 14, 22, 374, 597, 790, 1123
DdeI CTNAG 6 cut(s) 312, 384, 579, 725, 999, 1085
DpnI GATC 7 cut(s) 57, 68, 108, 765, 1083, 1127, 1134
DpnII GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
EaeI YGGCCR 1 cut(s) 293
EagI CGGCCG 1 cut(s) 293
EciI GGCGGA 1 cut(s) 1121
Ecl136II GAGCTC 1 cut(s) 406
EclXI CGGCCG 1 cut(s) 293
Eco105I TACGTA 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 457, 660
Eco52I CGGCCG 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 2 cut(s) 657, 1167
EcoICRI GAGCTC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 408
FaeI CATG 6 cut(s) 17, 25, 377, 600, 793, 1126
FaqI GGGAC 2 cut(s) 240, 945
FatI CATG 6 cut(s) 13, 21, 373, 596, 789, 1122
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 2 cut(s) 431, 1149
Fnu4HI GCNGC 2 cut(s) 308, 491
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 2 cut(s) 308, 491
FspBI CTAG 4 cut(s) 98, 743, 776, 1163
GlaI GCGC 2 cut(s) 1094, 1188
GluI GCNGC 2 cut(s) 308, 491
GsuI CTGGAG 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 1190
HaeIII GGCC 4 cut(s) 295, 366, 990, 1045
HapII CCGG 4 cut(s) 209, 292, 296, 761
HgaI GACGC 1 cut(s) 678
HhaI GCGC 2 cut(s) 1095, 1189
Hin1I GRCGYC 1 cut(s) 670
Hin1II CATG 6 cut(s) 17, 25, 377, 600, 793, 1126
Hin6I GCGC 2 cut(s) 1093, 1187
HinP1I GCGC 2 cut(s) 1093, 1187
HincII GTYRAC 2 cut(s) 231, 907
HindII GTYRAC 2 cut(s) 231, 907
HinfI GANTC 4 cut(s) 581, 834, 1155, 1173
HpaII CCGG 4 cut(s) 209, 292, 296, 761
HphI GGTGA 1 cut(s) 991
Hpy166II GTNNAC 9 cut(s) 231, 432, 602, 660, 715, 868, 907, 982, 1150
Hpy188I TCNGA 4 cut(s) 655, 819, 1086, 1130
Hpy188III TCNNGA 4 cut(s) 276, 761, 790, 1136
Hpy8I GTNNAC 9 cut(s) 231, 432, 602, 660, 715, 868, 907, 982, 1150
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 8 cut(s) 40, 356, 418, 633, 974, 1163, 1191, 1202
HpyCH4III ACNGT 4 cut(s) 64, 73, 712, 986
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 6 cut(s) 13, 114, 147, 373, 493, 883
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 313
HpyF3I CTNAG 6 cut(s) 312, 384, 579, 725, 999, 1085
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 1 cut(s) 670
Hsp92II CATG 6 cut(s) 17, 25, 377, 600, 793, 1126
HspAI GCGC 2 cut(s) 1093, 1187
Kpn2I TCCGGA 1 cut(s) 760
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
LmnI GCTCC 1 cut(s) 949
Lsp1109I GCAGC 2 cut(s) 319, 477
LweI GCATC 2 cut(s) 913, 1104
MaeI CTAG 4 cut(s) 98, 743, 776, 1163
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 379, 469, 589
MalI GATC 7 cut(s) 57, 68, 108, 765, 1083, 1127, 1134
MboI GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
MboII GAAGA 4 cut(s) 516, 611, 650, 910
MflI RGATCY 2 cut(s) 55, 1081
MhlI GDGCHC 3 cut(s) 350, 408, 1091
MlyI GAGTC 2 cut(s) 590, 1182
MroI TCCGGA 1 cut(s) 760
MseI TTAA 2 cut(s) 522, 647
MspI CCGG 4 cut(s) 209, 292, 296, 761
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 1 cut(s) 1093
MwoI GCNNNNNNNGC 2 cut(s) 47, 313
NdeII GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
NlaIII CATG 6 cut(s) 17, 25, 377, 600, 793, 1126
NmuCI GTSAC 1 cut(s) 379
NspI RCATGY 1 cut(s) 600
PagI TCATGA 1 cut(s) 789
PceI AGGCCT 1 cut(s) 366
PfeI GAWTC 2 cut(s) 834, 1155
PkrI GCNGC 2 cut(s) 309, 492
PleI GAGTC 2 cut(s) 589, 1181
PpsI GAGTC 2 cut(s) 589, 1181
Ppu21I YACGTR 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspPI GGNCC 3 cut(s) 457, 660, 989
PsuI RGATCY 2 cut(s) 55, 1081
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 2 cut(s) 522, 647
SatI GCNGC 2 cut(s) 308, 491
Sau3AI GATC 7 cut(s) 55, 66, 106, 763, 1081, 1125, 1132
Sau96I GGNCC 3 cut(s) 457, 660, 989
SchI GAGTC 2 cut(s) 590, 1182
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 3 cut(s) 350, 408, 1091
SfaNI GCATC 2 cut(s) 913, 1104
SfcI CTRYAG 1 cut(s) 1150
SinI GGWCC 2 cut(s) 457, 660
SmlI CTYRAG 2 cut(s) 401, 940
SmoI CTYRAG 2 cut(s) 401, 940
SnaBI TACGTA 1 cut(s) 184
SpeI ACTAGT 1 cut(s) 742
SseBI AGGCCT 1 cut(s) 366
SsiI CCGC 2 cut(s) 594, 1106
SspI AATATT 1 cut(s) 753
SspMI CTAG 4 cut(s) 98, 743, 776, 1163
SstI GAGCTC 1 cut(s) 408
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 4 cut(s) 64, 73, 712, 986
TaiI ACGT 1 cut(s) 186
TaqI TCGA 2 cut(s) 161, 967
TfiI GAWTC 2 cut(s) 834, 1155
Tru1I TTAA 2 cut(s) 522, 647
Tru9I TTAA 2 cut(s) 522, 647
TscAI CASTG 4 cut(s) 76, 640, 717, 1003
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 2 cut(s) 307, 490
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 5 cut(s) 369, 528, 855, 865, 1182
TspGWI ACGGA 2 cut(s) 179, 962
TspRI CASTG 4 cut(s) 76, 640, 717, 1003
VpaK11BI GGWCC 2 cut(s) 457, 660
VspI ATTAAT 1 cut(s) 522
XapI RAATTY 2 cut(s) 223, 338
XceI RCATGY 1 cut(s) 600
XmiI GTMKAC 2 cut(s) 431, 1149
XspI CTAG 4 cut(s) 98, 743, 776, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.