Rorug03G0109900

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0004386
Physical Location & Seq
Forward (+)
8815834 .. 8818041
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0109900.1

Sequence Viewer

Length: 366 bp
ATGGCTTCTCACAAGTTCTTCTCCATCACTGTTCTAACAGCTTTGCTCTCGCTCTGCACCAATGCTCTGTCTCGTACGGAATACAATTGTTCTTCTCAGAAAGTTAACCTAACTATATATTATGACACTCTTAACCCGTACTGTGAGGAATTCATTGTATACGAGCTTCCAAAGGCATTTGAGCTGGAGCTCATGCACATTATCAACCTCCGTCTAGTACCATATGGAAATGCTTACATCCAAGAACCTAACAACACCATCAGTTGCCAGGTCATTTTCCCACCTTCCTCATCTTGTTTTCTTTCTTTTTATGGTTTGTTGACATGGTGTTTCATATGTATATGCCCTGCAGAATGGCCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

121

Amino Acids

13.87

Weight (kDa)

4.92

Isoelectric Point (pI)

52.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 36 - 90 9.7e-10 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 159
AcsI RAATTY 1 cut(s) 149
AfaI GTAC 3 cut(s) 76, 140, 219
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 5 cut(s) 41, 166, 184, 190, 363
AluI AGCT 5 cut(s) 41, 166, 184, 190, 363
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 75
AoxI GGCC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 357
BanII GRGCYC 1 cut(s) 192
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
Bbv12I GWGCWC 1 cut(s) 192
BccI CCATC 2 cut(s) 32, 266
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 348
Bme1390I CCNGG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 269
BseGI GGATG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 110
BseYI CCCAGC 1 cut(s) 359
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 192
BsiWI CGTACG 1 cut(s) 74
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 192
BspANI GGCC 1 cut(s) 358
BspCNI CTCAG 1 cut(s) 109
BspMAI CTGCAG 1 cut(s) 352
BssNAI GTATAC 1 cut(s) 160
Bst1107I GTATAC 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 31, 143
BstDEI CTNAG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 237
BstMAI GTCTC 1 cut(s) 75
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 348
BstZ17I GTATAC 1 cut(s) 160
BsuRI GGCC 1 cut(s) 358
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 357
Csp6I GTAC 3 cut(s) 75, 139, 218
CviAII CATG 2 cut(s) 193, 324
CviJI RGCY 7 cut(s) 5, 41, 166, 184, 190, 358, 363
CviKI_1 RGCY 7 cut(s) 5, 41, 166, 184, 190, 358, 363
CviQI GTAC 3 cut(s) 75, 139, 218
DdeI CTNAG 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 2 cut(s) 196, 327
FatI CATG 2 cut(s) 192, 323
FauNDI CATATG 2 cut(s) 223, 335
FblI GTMKAC 1 cut(s) 159
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 192
FspBI CTAG 1 cut(s) 215
GsaI CCCAGC 1 cut(s) 363
GsuI CTGGAG 1 cut(s) 206
HaeIII GGCC 1 cut(s) 358
Hin1II CATG 2 cut(s) 196, 327
HincII GTYRAC 2 cut(s) 106, 321
HindII GTYRAC 2 cut(s) 106, 321
HpaI GTTAAC 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 106, 160, 321
Hpy188I TCNGA 1 cut(s) 99
Hpy8I GTNNAC 3 cut(s) 106, 160, 321
HpyAV CCTTC 1 cut(s) 294
HpyCH4III ACNGT 2 cut(s) 31, 143
HpyCH4V TGCA 3 cut(s) 57, 196, 350
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 2 cut(s) 196, 327
KspAI GTTAAC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 187
LpnPI CCDG 4 cut(s) 170, 254, 281, 360
MaeI CTAG 1 cut(s) 215
MboII GAAGA 2 cut(s) 10, 84
MfeI CAATTG 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 85, 149
MnlI CCTC 3 cut(s) 139, 218, 298
MseI TTAA 2 cut(s) 105, 132
MspA1I CMGCKG 1 cut(s) 363
MspR9I CCNGG 1 cut(s) 269
MunI CAATTG 1 cut(s) 85
MvaI CCWGG 1 cut(s) 269
NdeI CATATG 2 cut(s) 223, 335
NlaIII CATG 2 cut(s) 196, 327
Pfl23II CGTACG 1 cut(s) 74
Psp124BI GAGCTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 267
PspLI CGTACG 1 cut(s) 74
PspPI GGNCC 1 cut(s) 357
PstI CTGCAG 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 363
RsaI GTAC 3 cut(s) 76, 140, 219
RsaNI GTAC 3 cut(s) 75, 139, 218
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 2 cut(s) 105, 132
Sau96I GGNCC 1 cut(s) 357
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 1 cut(s) 192
SfcI CTRYAG 1 cut(s) 348
Sse9I AATT 2 cut(s) 85, 149
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 31, 143
TasI AATT 2 cut(s) 85, 149
Tru1I TTAA 2 cut(s) 105, 132
Tru9I TTAA 2 cut(s) 105, 132
TscAI CASTG 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 142, 322
TspGWI ACGGA 2 cut(s) 92, 200
TspRI CASTG 1 cut(s) 34
XapI RAATTY 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.