Rroxscaffold_6G00410740

ACT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004386
Physical Location & Seq
Reverse (-)
33142091 .. 33145506
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410740.1

Sequence Viewer

Length: 1239 bp
ATGGGTATTCTACACGACGATGTGGTAATCATCACAGAGCCAGAGAAACAAGGCGACCCAAGTGTGATTACCATAAATTGCCCAGACAAAACCGGCTTGGGCTGTGACTTGTGTCGCATCATTCTCTTCTTTGGTCTTAGCATTGTCAGAGGAGATGTATCAACGGATGGGAAGTGGTGCTTCTTAGTGTTTTGGGTGGTTGGGAACTCAGGAACAAGGTGGGATTTGTTGAAGAGGAGGTTAATGGAGACTTGTCCATCTTTTTCTTCAGCTTCTGGGATTTCCTTTTACCGCTATGAGTTGCAGCCTCCAAAGCCTCCAGATGTGTTCCTTCTCAAGTTCTGTTGTTATGATCGGAGAGGGATTTTACATGATGTGACAGAGGTTCTATGTGAACTTGAGCTCACCATAAAGAAAGTCAAGGTATCCACCACCCCGGATGGGAAAGTGATGGACCTGTTTTTCGTCACAGACACTAGGGAACTTCTGCATACAAATAAGAGAAAGGATGAGGTATCTGACCACTTAAAAGCTGCTACGGGAAATGCCATGATAAGTTTTGATATTGAAATGGTTGGTCCAGAGATTACTGCATGTTCACAGTCATCTTCATTTCTCCCATCTGTTCTTATGGAGGACATGTTTCATTTGGAAATGCCTGATGAACTCCCAAGTGGAGTAGTCACCTCTAGCTGTGTTTCTGTGAATATGGACAATTCGCTCAGTCCTGGCCACACACTGGTCCAAATCGTTTGCCAAGATCACAAAGGCCTCCTTTATGATATAATGAGAACTCTAAAGGATTATAATATTCAGATCTCTTATGGGCGCTTCTCTACAAAACAAAGAAGAAACTGTGAGATTGACTTGTTCATCGTGCAAGCTGATGGAAAGAAGATAGTTGATCCAAGCAAGCAGAATGCGTTGACATCTCGTTTGCAGATGGAACTACTTCGTCCTCTCAGAGTAGCTGTTGTTAGCAAAGGTCCTGATACAGAGCTCCTGGTTGCTAATCCGGTGGAATTATCTGGGAAAGGGCGGCCTCTTGTTTTCCATGATGTAACCCTTGCTCTCAAGATGCTGGACACTGGCATATTTTCGGCTAAGATTGGAAGGCATTTAGTTAAAGATCGGGAATGGGAAGTTTATAGAGTCTTGCTTGATGAAGGAGATGGCTTGCCAGTTCCAAGGAACAAGATTGAAGAAGGAGTTTGGAAGCTGTTGATGGGTTGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ACT

Protein Analysis

412

Amino Acids

46.31

Weight (kDa)

5.69

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 12 - 86 7e-39 ACT domain-containing protein ACR10, N-terminal
ACT PF01842 112 - 167 5.1e-06 ACT domain
ACT_ACR9_3rd PF24931 234 - 331 4.9e-50 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 332 - 410 5.1e-37 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB7I CCANNNNNTGG 1 cut(s) 739
AciI CCGC 2 cut(s) 292, 1039
AclWI GGATC 1 cut(s) 899
AcoI YGGCCR 1 cut(s) 730
AcuI CTGAAG 1 cut(s) 252
AfiI CCNNNNNNNGG 2 cut(s) 441, 739
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 3 cut(s) 232, 569, 1202
AjnI CCWGG 2 cut(s) 727, 1002
AluBI AGCT 8 cut(s) 272, 403, 533, 693, 884, 971, 1000, 1219
AluI AGCT 8 cut(s) 272, 403, 533, 693, 884, 971, 1000, 1219
Alw21I GWGCWC 2 cut(s) 405, 1002
Alw26I GTCTC 1 cut(s) 242
AlwI GGATC 1 cut(s) 899
AlwNI CAGNNNCTG 1 cut(s) 275
AoxI GGCC 3 cut(s) 730, 769, 1040
ApeKI GCWGC 2 cut(s) 304, 533
ArsI GACNNNNNNTTYG 4 cut(s) 446, 478, 919, 951
Asp700I GAANNNNTTC 1 cut(s) 951
AspLEI GCGC 1 cut(s) 831
AspS9I GGNCC 4 cut(s) 454, 578, 742, 986
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 397, 676
AvaII GGWCC 4 cut(s) 454, 578, 742, 986
BalI TGGCCA 1 cut(s) 732
BanII GRGCYC 2 cut(s) 405, 1002
Bbv12I GWGCWC 2 cut(s) 405, 1002
BbvI GCAGC 2 cut(s) 316, 520
BccI CCATC 9 cut(s) 161, 265, 434, 445, 628, 881, 937, 1166, 1219
BciT130I CCWGG 2 cut(s) 729, 1004
BciVI GTATCC 1 cut(s) 436
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 2 cut(s) 477, 690
BfoI RGCGCY 1 cut(s) 832
BfuI GTATCC 1 cut(s) 436
BglII AGATCT 1 cut(s) 816
BisI GCNGC 3 cut(s) 305, 534, 1040
BlsI GCNGC 3 cut(s) 306, 535, 1041
Bme1390I CCNGG 3 cut(s) 437, 729, 1004
Bme18I GGWCC 4 cut(s) 454, 578, 742, 986
BmgT120I GGNCC 4 cut(s) 454, 578, 742, 986
BmrFI CCNGG 3 cut(s) 437, 729, 1004
BmsI GCATC 2 cut(s) 126, 1068
BoxI GACNNNNGTC 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 303
BpuEI CTTGAG 3 cut(s) 320, 419, 1058
BpuMI CCSGG 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 435, 1187
BsaWI WCCGGW 1 cut(s) 1015
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 739
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 3 cut(s) 744, 1093, 1181
BseBI CCWGG 2 cut(s) 729, 1004
BseDI CCNNGG 2 cut(s) 435, 1187
BseGI GGATG 3 cut(s) 172, 445, 514
BseLI CCNNNNNNNGG 2 cut(s) 441, 739
BseMII CTCAG 3 cut(s) 222, 736, 976
BseNI ACTGG 3 cut(s) 744, 1093, 1181
BseRI GAGGAG 2 cut(s) 165, 250
BseXI GCAGC 2 cut(s) 316, 520
BshFI GGCC 3 cut(s) 732, 771, 1042
BsiHKAI GWGCWC 2 cut(s) 405, 1002
BsiSI CCGG 3 cut(s) 93, 437, 1016
BslI CCNNNNNNNGG 2 cut(s) 441, 739
BsmAI GTCTC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 925
BsnI GGCC 3 cut(s) 732, 771, 1042
Bsp1286I GDGCHC 2 cut(s) 405, 1002
Bsp143I GATC 5 cut(s) 352, 760, 816, 904, 1129
BspACI CCGC 2 cut(s) 292, 1039
BspANI GGCC 3 cut(s) 732, 771, 1042
BspCNI CTCAG 3 cut(s) 221, 735, 975
BspPI GGATC 1 cut(s) 899
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 3 cut(s) 744, 1093, 1181
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 435, 1187
BssMI GATC 5 cut(s) 352, 760, 816, 904, 1129
BssT1I CCWWGG 1 cut(s) 1187
Bst2UI CCWGG 2 cut(s) 729, 1004
Bst4CI ACNGT 2 cut(s) 603, 857
Bst6I CTCTTC 2 cut(s) 131, 227
BstC8I GCNNGC 3 cut(s) 882, 914, 1178
BstDEI CTNAG 6 cut(s) 137, 184, 208, 722, 962, 1104
BstF5I GGATG 3 cut(s) 172, 445, 514
BstH2I RGCGCY 1 cut(s) 832
BstHHI GCGC 1 cut(s) 831
BstKTI GATC 5 cut(s) 355, 763, 819, 907, 1132
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 5 cut(s) 352, 760, 816, 904, 1129
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstNI CCWGG 2 cut(s) 729, 1004
BstNSI RCATGY 2 cut(s) 597, 643
BstPAI GACNNNNGTC 1 cut(s) 111
BstSCI CCNGG 3 cut(s) 435, 727, 1002
BstV1I GCAGC 2 cut(s) 316, 520
BstX2I RGATCY 1 cut(s) 816
BstYI RGATCY 1 cut(s) 816
BsuI GTATCC 1 cut(s) 436
BsuRI GGCC 3 cut(s) 732, 771, 1042
BtsCI GGATG 3 cut(s) 172, 445, 514
BtsIMutI CAGTG 2 cut(s) 737, 1086
Cac8I GCNNGC 3 cut(s) 882, 914, 1178
CaiI CAGNNNCTG 1 cut(s) 275
CfoI GCGC 1 cut(s) 831
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 4 cut(s) 454, 578, 742, 986
CviAII CATG 5 cut(s) 371, 550, 594, 640, 1055
DdeI CTNAG 6 cut(s) 137, 184, 208, 722, 962, 1104
DpnI GATC 5 cut(s) 354, 762, 818, 906, 1131
DpnII GATC 5 cut(s) 352, 760, 816, 904, 1129
EaeI YGGCCR 1 cut(s) 730
Eam1104I CTCTTC 2 cut(s) 131, 227
EarI CTCTTC 2 cut(s) 131, 227
Ecl136II GAGCTC 2 cut(s) 403, 1000
Eco130I CCWWGG 1 cut(s) 1187
Eco147I AGGCCT 1 cut(s) 771
Eco24I GRGCYC 2 cut(s) 405, 1002
Eco47I GGWCC 4 cut(s) 454, 578, 742, 986
Eco53kI GAGCTC 2 cut(s) 403, 1000
Eco57I CTGAAG 1 cut(s) 252
EcoICRI GAGCTC 2 cut(s) 403, 1000
EcoO109I RGGNCCY 1 cut(s) 986
EcoRII CCWGG 2 cut(s) 727, 1002
EcoT14I CCWWGG 1 cut(s) 1187
EcoT38I GRGCYC 2 cut(s) 405, 1002
ErhI CCWWGG 1 cut(s) 1187
FaeI CATG 5 cut(s) 374, 553, 597, 643, 1058
FalI AAGNNNNNCTT 4 cut(s) 164, 196, 759, 791
FatI CATG 5 cut(s) 370, 549, 593, 639, 1054
Fnu4HI GCNGC 3 cut(s) 305, 534, 1040
FokI GGATG 3 cut(s) 179, 452, 521
FriOI GRGCYC 2 cut(s) 405, 1002
Fsp4HI GCNGC 3 cut(s) 305, 534, 1040
FspBI CTAG 2 cut(s) 477, 690
GlaI GCGC 1 cut(s) 830
GluI GCNGC 3 cut(s) 305, 534, 1040
GsuI CTGGAG 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 832
HaeIII GGCC 3 cut(s) 732, 771, 1042
HapII CCGG 3 cut(s) 93, 437, 1016
HhaI GCGC 1 cut(s) 831
Hin1II CATG 5 cut(s) 374, 553, 597, 643, 1058
Hin6I GCGC 1 cut(s) 829
HinP1I GCGC 1 cut(s) 829
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HinfI GANTC 1 cut(s) 1152
HpaII CCGG 3 cut(s) 93, 437, 1016
HphI GGTGA 2 cut(s) 397, 676
Hpy166II GTNNAC 3 cut(s) 395, 599, 927
Hpy188I TCNGA 5 cut(s) 149, 357, 520, 816, 965
Hpy188III TCNNGA 6 cut(s) 210, 320, 581, 989, 1075, 1133
Hpy8I GTNNAC 3 cut(s) 395, 599, 927
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 341, 1107, 1160, 1199
HpyCH4III ACNGT 2 cut(s) 603, 857
HpyCH4V TGCA 5 cut(s) 304, 490, 593, 880, 940
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 6 cut(s) 137, 184, 208, 722, 962, 1104
Hsp92II CATG 5 cut(s) 374, 553, 597, 643, 1058
HspAI GCGC 1 cut(s) 829
Kzo9I GATC 5 cut(s) 352, 760, 816, 904, 1129
LmnI GCTCC 1 cut(s) 1005
Lsp1109I GCAGC 2 cut(s) 316, 520
LweI GCATC 2 cut(s) 126, 1068
MaeI CTAG 2 cut(s) 477, 690
MaeIII GTNAC 5 cut(s) 104, 376, 466, 682, 1060
MalI GATC 5 cut(s) 354, 762, 818, 906, 1131
MboI GATC 5 cut(s) 352, 760, 816, 904, 1129
MboII GAAGA 7 cut(s) 118, 244, 258, 600, 861, 907, 1214
MflI RGATCY 1 cut(s) 816
MhlI GDGCHC 2 cut(s) 405, 1002
MlsI TGGCCA 1 cut(s) 732
MluCI AATT 3 cut(s) 76, 715, 1022
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 1 cut(s) 1161
Mox20I TGGCCA 1 cut(s) 732
MroXI GAANNNNTTC 1 cut(s) 951
MscI TGGCCA 1 cut(s) 732
MseI TTAA 3 cut(s) 242, 527, 1125
MslI CAYNNNNRTG 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 3 cut(s) 93, 437, 1016
MspR9I CCNGG 3 cut(s) 437, 729, 1004
Mva1269I GAATGC 1 cut(s) 925
MvaI CCWGG 2 cut(s) 729, 1004
MwoI GCNNNNNNNGC 1 cut(s) 313
NciI CCSGG 1 cut(s) 437
NdeII GATC 5 cut(s) 352, 760, 816, 904, 1129
NlaIII CATG 5 cut(s) 374, 553, 597, 643, 1058
NmuCI GTSAC 4 cut(s) 104, 376, 466, 682
NspI RCATGY 2 cut(s) 597, 643
PceI AGGCCT 1 cut(s) 771
PciI ACATGT 1 cut(s) 639
PctI GAATGC 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 951
PflMI CCANNNNNTGG 1 cut(s) 739
PkrI GCNGC 3 cut(s) 306, 535, 1041
PleI GAGTC 1 cut(s) 1160
PpsI GAGTC 1 cut(s) 1160
PpuMI RGGWCCY 1 cut(s) 986
PscI ACATGT 1 cut(s) 639
PshAI GACNNNNGTC 1 cut(s) 111
PsiI TTATAA 1 cut(s) 807
Psp124BI GAGCTC 2 cut(s) 405, 1002
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 2 cut(s) 727, 1002
PspGI CCWGG 2 cut(s) 727, 1002
PspPI GGNCC 4 cut(s) 454, 578, 742, 986
PspPPI RGGWCCY 1 cut(s) 986
PstNI CAGNNNCTG 1 cut(s) 275
PsuI RGATCY 1 cut(s) 816
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 2 cut(s) 405, 1002
SaqAI TTAA 3 cut(s) 242, 527, 1125
SatI GCNGC 3 cut(s) 305, 534, 1040
Sau3AI GATC 5 cut(s) 352, 760, 816, 904, 1129
Sau96I GGNCC 4 cut(s) 454, 578, 742, 986
SchI GAGTC 1 cut(s) 1161
ScrFI CCNGG 3 cut(s) 437, 729, 1004
SduI GDGCHC 2 cut(s) 405, 1002
SfaNI GCATC 2 cut(s) 126, 1068
SinI GGWCC 4 cut(s) 454, 578, 742, 986
SmiMI CAYNNNNRTG 1 cut(s) 18
SmlI CTYRAG 3 cut(s) 335, 398, 1073
SmoI CTYRAG 3 cut(s) 335, 398, 1073
Sse9I AATT 3 cut(s) 76, 715, 1022
SseBI AGGCCT 1 cut(s) 771
SsiI CCGC 2 cut(s) 292, 1039
SspI AATATT 1 cut(s) 811
SspMI CTAG 2 cut(s) 477, 690
SstI GAGCTC 2 cut(s) 405, 1002
StuI AGGCCT 1 cut(s) 771
StyD4I CCNGG 3 cut(s) 435, 727, 1002
StyI CCWWGG 1 cut(s) 1187
TaaI ACNGT 2 cut(s) 603, 857
TasI AATT 3 cut(s) 76, 715, 1022
TauI GCSGC 1 cut(s) 1042
Tru1I TTAA 3 cut(s) 242, 527, 1125
Tru9I TTAA 3 cut(s) 242, 527, 1125
TscAI CASTG 2 cut(s) 744, 1093
TseFI GTSAC 4 cut(s) 104, 376, 466, 682
TseI GCWGC 2 cut(s) 304, 533
Tsp45I GTSAC 4 cut(s) 104, 376, 466, 682
TspDTI ATGAA 5 cut(s) 600, 635, 678, 862, 1179
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 2 cut(s) 744, 1093
Van91I CCANNNNNTGG 1 cut(s) 739
VpaK11BI GGWCC 4 cut(s) 454, 578, 742, 986
XceI RCATGY 2 cut(s) 597, 643
XmnI GAANNNNTTC 1 cut(s) 951
XspI CTAG 2 cut(s) 477, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.