FvH4_6g20770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001246
Physical Location & Seq
Forward (+)
14310184 .. 14316323
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20770.t12

Sequence Viewer

Length: 1800 bp
ATGGAGAAAAAGAGAAAGATCACACAATACAGGGAGAGGTTGGACAAGACATTGGCATCTCCAAATCTAACAAACAAGGAGGCTTTAAAAGTCCTTGTCAAGGATCAGCTTCTCCGCTCTTCAGAAAATGAAATTAAAGGTTGTAATGCAAACATTATCGAAGAAAGGACGGCTGAAGTGTCAAATTTGCTTGACATGTTGAGGAGTGCTTCCCTTGGTGATGACGAGGGATTGAAAACTTGTGATACTCCATCACAGCCTGAATGGAAATTAAAACAGGACAATGAGGAGTTCCGGGTTATGTATCGTGAGGGAATCCAAGGCAGTCCATTTCATACATTACTTGTTGAAGGTTACATAGATGGGCCTGTTGATGTCTGTTTATGCATCTCGTGTGAGGCAGAACTTTACAAGAAATGGTGGCCTCAGTCTACCATTCCAACTTTCAAAATTCTCTCTGGGAAGTGTTTGCAGAAGGTTGGCATCGGTGAACATGTATCTGTTGTGAGGATGAAGATTCCGTGGCCACTTTCAGCTAGGGAGGCTGTAGTGCACTATTTTGTGTTCGAGTATTATGAAGATGACCTGATTGTTTGTCTCTTGAACACGATCTCTGATTTTGAAGGCATTGAAGGTGGCCTAAAAAATGAGGCAACTGATGTAGCAAAGGATGTGGTTAGGATAGATGTAGTAGGTGGGTTTGCGTTGCAGAAGGTTACTGAAGATCGAAGTTACTTTCGGACGATAGCAAATATGGATATAAAGATGGACTTAATCCCACCATCCCTTATAAATTTTGTCTCAAGGCAGCTCATTGGGAACGGTTTCAGACTATATCAAAAGGTAGTGTCTTCAAAGCTTAATCGTGATGAAGATTACAGCAAGGCTTTGAGAGGCCCACTATACTCTCGCATACGTGAAGCTCTTTCTTCACTTCATGAATCAAAAAGGCTTGTGGAGGGAAAAAAGCTACATGATGATGCATCCAATCTCTTTGAAGAACATCTAACTAAAAATAAAATGAATGATTTGAAGTTGGTAAATATGGATGAGAAAGTTCATAATGAACTACTTGCAAGTGAATCTACCCCAGATGATGCACATTTTACCGGAGGAAACACTTGTGAGATTGAGGAGGTAGAGAGTGAAGAAAGCAAGACTGAGGAGATAGAAAGCGAAAAAAGCAAGATTGAGGAGGTACAGAGCAAAGAAAGCAGGCAGCTTGAGGATCAAACATCCAACAGAATTCCAGAAAGGGGACATATAAATGGCAAGAGAAACGTGTTTATCAGTCCTGAGGTGGAACAAGCTATAAGAACAATAGACAAGGTCATTTTGAAGGTTCGCCAATATAGGTTCAGTGCTGAAATGCCGTCCTCTGGTTTCAACACTGAAGTCTCAGGAAAGGAAAAGGATGGAGGTAATCCGAAGTCCTTAGAAGATGAAATCCATTCAAGTGAACATCTTTCTGCAGTACCTAAACCAGAGGACACTGAAGGGGCGTTAACTACACCTTTAAGGAGTAGCACTGCCATTCAAAACATAAGTTATGCAGGCTCAAATTATTTGTCAAAGGAAGTTAACCATAACAGAATAGTACCTACTTCCCCATTGTGTTCGTCCAAAGATGGGGCTGCAGAGGTACCTGTAGTGGATAACATCATGCATGGTACCGACCAAGTTAGCTGCAAAGAAGATGACACTCATCAAGGTAGTCCCAGGAAAAGGAAGATGTCAAGGCGGCAAATAAGGCGTGGGTTGTGTTGCTTCAGCATCAATTCAGGACAGATAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

600

Amino Acids

67.59

Weight (kDa)

5.54

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
Acc65I GGTACC 2 cut(s) 1642, 1670
AccB1I GGYRCC 2 cut(s) 1642, 1670
AccBSI CCGCTC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 431
AciI CCGC 2 cut(s) 115, 1741
AclWI GGATC 2 cut(s) 111, 1236
AcoI YGGCCR 1 cut(s) 524
AcsI RAATTY 4 cut(s) 184, 450, 793, 1245
AcuI CTGAAG 6 cut(s) 105, 195, 741, 1413, 1515, 1753
AfaI GTAC 5 cut(s) 1200, 1476, 1599, 1644, 1672
AfiI CCNNNNNNNGG 5 cut(s) 100, 1256, 1379, 1629, 1725
AflIII ACRYGT 3 cut(s) 195, 493, 1281
AjnI CCWGG 1 cut(s) 1718
AluBI AGCT 9 cut(s) 109, 536, 811, 859, 923, 970, 1222, 1310, 1686
AluI AGCT 9 cut(s) 109, 536, 811, 859, 923, 970, 1222, 1310, 1686
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 3 cut(s) 602, 805, 1402
Alw44I GTGCAC 1 cut(s) 551
AlwI GGATC 2 cut(s) 111, 1236
AoxI GGCC 5 cut(s) 365, 422, 524, 637, 895
ApaLI GTGCAC 1 cut(s) 551
ApeKI GCWGC 4 cut(s) 808, 1219, 1634, 1686
ApoI RAATTY 4 cut(s) 184, 450, 793, 1245
ArsI GACNNNNNNTTYG 2 cut(s) 1553, 1585
Asp700I GAANNNNTTC 1 cut(s) 824
Asp718I GGTACC 2 cut(s) 1642, 1670
AspS9I GGNCC 2 cut(s) 365, 896
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 2 cut(s) 230, 500
AxyI CCTNAGG 1 cut(s) 1296
BaeGI GKGCMC 1 cut(s) 555
BalI TGGCCA 1 cut(s) 526
BanI GGYRCC 2 cut(s) 1642, 1670
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 4 cut(s) 820, 1231, 1621, 1673
BccI CCATC 6 cut(s) 259, 356, 760, 790, 1409, 1622
BceAI ACGGC 2 cut(s) 186, 1357
BciT130I CCWGG 1 cut(s) 1720
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 3 cut(s) 602, 805, 1402
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 4 cut(s) 546, 1470, 1635, 1647
BisI GCNGC 5 cut(s) 809, 1220, 1635, 1687, 1742
BlsI GCNGC 5 cut(s) 810, 1221, 1636, 1688, 1743
Bme1390I CCNGG 2 cut(s) 296, 1720
BmgT120I GGNCC 2 cut(s) 365, 896
BmiI GGNNCC 2 cut(s) 1644, 1672
BmrFI CCNGG 2 cut(s) 296, 1720
BmsI GCATC 7 cut(s) 65, 396, 492, 970, 992, 1087, 1782
BpiI GAAGAC 1 cut(s) 843
BpuEI CTTGAG 2 cut(s) 787, 1244
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 1 cut(s) 917
BsaJI CCNNGG 4 cut(s) 214, 319, 521, 1718
BsaWI WCCGGW 1 cut(s) 1109
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 1256, 1379, 1629, 1725
Bse21I CCTNAGG 1 cut(s) 1296
BseBI CCWGG 1 cut(s) 1720
BseDI CCNNGG 4 cut(s) 214, 319, 521, 1718
BseGI GGATG 7 cut(s) 516, 676, 782, 983, 1054, 1235, 1420
BseLI CCNNNNNNNGG 5 cut(s) 100, 1256, 1379, 1629, 1725
BseMII CTCAG 4 cut(s) 440, 1152, 1287, 1413
BseRI GAGGAG 5 cut(s) 217, 302, 1148, 1178, 1208
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 4 cut(s) 820, 1231, 1621, 1673
BshFI GGCC 5 cut(s) 367, 424, 526, 639, 897
BshNI GGYRCC 2 cut(s) 1642, 1670
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 295, 1110
BslFI GGGAC 2 cut(s) 1272, 1701
BslI CCNNNNNNNGG 5 cut(s) 100, 1256, 1379, 1629, 1725
BsmAI GTCTC 3 cut(s) 602, 805, 1402
BsmFI GGGAC 2 cut(s) 1272, 1701
BsnI GGCC 5 cut(s) 367, 424, 526, 639, 897
Bsp1286I GDGCHC 1 cut(s) 555
Bsp143I GATC 5 cut(s) 18, 103, 609, 724, 1228
BspACI CCGC 2 cut(s) 115, 1741
BspANI GGCC 5 cut(s) 367, 424, 526, 639, 897
BspCNI CTCAG 4 cut(s) 439, 1153, 1288, 1412
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 1644, 1672
BspMAI CTGCAG 2 cut(s) 1474, 1639
BspPI GGATC 2 cut(s) 111, 1236
BspQI GCTCTTC 1 cut(s) 124
BspT107I GGYRCC 2 cut(s) 1642, 1670
BsrBI CCGCTC 1 cut(s) 117
BssECI CCNNGG 4 cut(s) 214, 319, 521, 1718
BssMI GATC 5 cut(s) 18, 103, 609, 724, 1228
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 2 cut(s) 214, 319
Bst2BI CACGAG 1 cut(s) 391
Bst2UI CCWGG 1 cut(s) 1720
Bst4CI ACNGT 1 cut(s) 824
Bst6I CTCTTC 1 cut(s) 124
BstBAI YACGTR 1 cut(s) 917
BstC8I GCNNGC 2 cut(s) 1217, 1555
BstDEI CTNAG 5 cut(s) 426, 1161, 1296, 1399, 1435
BstDSI CCRYGG 1 cut(s) 521
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 7 cut(s) 516, 676, 782, 983, 1054, 1235, 1420
BstKTI GATC 5 cut(s) 21, 106, 612, 727, 1231
BstMAI GTCTC 3 cut(s) 602, 805, 1402
BstMBI GATC 5 cut(s) 18, 103, 609, 724, 1228
BstMWI GCNNNNNNNGC 4 cut(s) 542, 1182, 1212, 1750
BstNI CCWGG 1 cut(s) 1720
BstNSI RCATGY 2 cut(s) 199, 497
BstSCI CCNGG 2 cut(s) 294, 1718
BstSFI CTRYAG 4 cut(s) 546, 1470, 1635, 1647
BstSLI GKGCMC 1 cut(s) 555
BstV1I GCAGC 4 cut(s) 820, 1231, 1621, 1673
BstV2I GAAGAC 1 cut(s) 843
Bsu36I CCTNAGG 1 cut(s) 1296
BsuRI GGCC 5 cut(s) 367, 424, 526, 639, 897
BtgI CCRYGG 1 cut(s) 521
BtsCI GGATG 7 cut(s) 516, 676, 782, 983, 1054, 1235, 1420
BtsI GCAGTG 1 cut(s) 1527
BtsIMutI CAGTG 4 cut(s) 1366, 1389, 1491, 1527
Cac8I GCNNGC 2 cut(s) 1217, 1555
CciI TCATGA 1 cut(s) 937
Cfr13I GGNCC 2 cut(s) 365, 896
Csp6I GTAC 5 cut(s) 1199, 1475, 1598, 1643, 1671
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviAII CATG 6 cut(s) 196, 494, 938, 974, 1663, 1667
CviQI GTAC 5 cut(s) 1199, 1475, 1598, 1643, 1671
DdeI CTNAG 5 cut(s) 426, 1161, 1296, 1399, 1435
DpnI GATC 5 cut(s) 20, 105, 611, 726, 1230
DpnII GATC 5 cut(s) 18, 103, 609, 724, 1228
DraI TTTAAA 1 cut(s) 87
EaeI YGGCCR 1 cut(s) 524
Eam1104I CTCTTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 124
Eco130I CCWWGG 2 cut(s) 214, 319
Eco57I CTGAAG 6 cut(s) 105, 195, 741, 1413, 1515, 1753
Eco81I CCTNAGG 1 cut(s) 1296
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 1245
EcoRII CCWGG 1 cut(s) 1718
EcoT14I CCWWGG 2 cut(s) 214, 319
EcoT22I ATGCAT 3 cut(s) 389, 985, 1668
ErhI CCWWGG 2 cut(s) 214, 319
FaeI CATG 6 cut(s) 199, 497, 941, 977, 1666, 1670
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 2 cut(s) 1272, 1701
FatI CATG 6 cut(s) 195, 493, 937, 973, 1662, 1666
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 5 cut(s) 809, 1220, 1635, 1687, 1742
FokI GGATG 7 cut(s) 523, 683, 769, 970, 1061, 1222, 1427
Fsp4HI GCNGC 5 cut(s) 809, 1220, 1635, 1687, 1742
FspBI CTAG 1 cut(s) 537
GluI GCNGC 5 cut(s) 809, 1220, 1635, 1687, 1742
HaeIII GGCC 5 cut(s) 367, 424, 526, 639, 897
HapII CCGG 2 cut(s) 295, 1110
Hin1II CATG 6 cut(s) 199, 497, 941, 977, 1666, 1670
HincII GTYRAC 2 cut(s) 1506, 1582
HindII GTYRAC 2 cut(s) 1506, 1582
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 5 cut(s) 315, 517, 941, 1082, 1793
HpaI GTTAAC 2 cut(s) 1506, 1582
HpaII CCGG 2 cut(s) 295, 1110
HphI GGTGA 2 cut(s) 230, 500
Hpy166II GTNNAC 6 cut(s) 432, 491, 553, 1460, 1506, 1582
Hpy188I TCNGA 5 cut(s) 124, 616, 741, 830, 1428
Hpy188III TCNNGA 8 cut(s) 308, 601, 866, 938, 1250, 1295, 1401, 1782
Hpy8I GTNNAC 6 cut(s) 432, 491, 553, 1460, 1506, 1582
HpyAV CCTTC 7 cut(s) 344, 469, 617, 626, 706, 1333, 1490
HpyCH4III ACNGT 1 cut(s) 824
HpyCH4IV ACGT 2 cut(s) 916, 1281
HpyF10VI GCNNNNNNNGC 4 cut(s) 542, 1182, 1212, 1750
HpyF3I CTNAG 5 cut(s) 426, 1161, 1296, 1399, 1435
HpySE526I ACGT 2 cut(s) 916, 1281
Hsp92II CATG 6 cut(s) 199, 497, 941, 977, 1666, 1670
KpnI GGTACC 2 cut(s) 1646, 1674
KspAI GTTAAC 2 cut(s) 1506, 1582
Kzo9I GATC 5 cut(s) 18, 103, 609, 724, 1228
LguI GCTCTTC 1 cut(s) 124
Lsp1109I GCAGC 4 cut(s) 820, 1231, 1621, 1673
LweI GCATC 7 cut(s) 65, 396, 492, 970, 992, 1087, 1782
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 916, 1281
MaeIII GTNAC 3 cut(s) 353, 715, 731
MalI GATC 5 cut(s) 20, 105, 611, 726, 1230
MbiI CCGCTC 1 cut(s) 117
MboI GATC 5 cut(s) 18, 103, 609, 724, 1228
MhlI GDGCHC 1 cut(s) 555
MlsI TGGCCA 1 cut(s) 526
MluCI AATT 8 cut(s) 132, 184, 269, 450, 793, 1245, 1561, 1777
MluNI TGGCCA 1 cut(s) 526
MmeI TCCRAC 3 cut(s) 21, 464, 1263
Mox20I TGGCCA 1 cut(s) 526
Mph1103I ATGCAT 3 cut(s) 389, 985, 1668
MroXI GAANNNNTTC 1 cut(s) 824
MscI TGGCCA 1 cut(s) 526
MseI TTAA 9 cut(s) 86, 135, 272, 773, 861, 1505, 1517, 1581, 1798
MslI CAYNNNNRTG 3 cut(s) 978, 1266, 1455
Msp20I TGGCCA 1 cut(s) 526
MspI CCGG 2 cut(s) 295, 1110
MspR9I CCNGG 2 cut(s) 296, 1720
MvaI CCWGG 1 cut(s) 1720
MwoI GCNNNNNNNGC 4 cut(s) 542, 1182, 1212, 1750
NciI CCSGG 1 cut(s) 296
NdeII GATC 5 cut(s) 18, 103, 609, 724, 1228
NlaIII CATG 6 cut(s) 199, 497, 941, 977, 1666, 1670
NlaIV GGNNCC 2 cut(s) 1644, 1672
NsiI ATGCAT 3 cut(s) 389, 985, 1668
NspI RCATGY 2 cut(s) 199, 497
PagI TCATGA 1 cut(s) 937
PciI ACATGT 2 cut(s) 195, 493
PciSI GCTCTTC 1 cut(s) 124
PdmI GAANNNNTTC 1 cut(s) 824
PfeI GAWTC 5 cut(s) 315, 517, 941, 1082, 1793
PflFI GACNNNGTC 1 cut(s) 1328
PkrI GCNGC 5 cut(s) 810, 1221, 1636, 1688, 1743
Ppu21I YACGTR 1 cut(s) 917
PscI ACATGT 2 cut(s) 195, 493
PsiI TTATAA 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 1718
PspGI CCWGG 1 cut(s) 1718
PspN4I GGNNCC 2 cut(s) 1644, 1672
PspPI GGNCC 2 cut(s) 365, 896
PstI CTGCAG 2 cut(s) 1474, 1639
PsyI GACNNNGTC 1 cut(s) 1328
RsaI GTAC 5 cut(s) 1200, 1476, 1599, 1644, 1672
RsaNI GTAC 5 cut(s) 1199, 1475, 1598, 1643, 1671
RseI CAYNNNNRTG 3 cut(s) 978, 1266, 1455
SapI GCTCTTC 1 cut(s) 124
SaqAI TTAA 9 cut(s) 86, 135, 272, 773, 861, 1505, 1517, 1581, 1798
SatI GCNGC 5 cut(s) 809, 1220, 1635, 1687, 1742
Sau3AI GATC 5 cut(s) 18, 103, 609, 724, 1228
Sau96I GGNCC 2 cut(s) 365, 896
ScrFI CCNGG 2 cut(s) 296, 1720
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 7 cut(s) 65, 396, 492, 970, 992, 1087, 1782
SfcI CTRYAG 4 cut(s) 546, 1470, 1635, 1647
SmiMI CAYNNNNRTG 3 cut(s) 978, 1266, 1455
SmlI CTYRAG 2 cut(s) 802, 1223
SmoI CTYRAG 2 cut(s) 802, 1223
Sse9I AATT 8 cut(s) 132, 184, 269, 450, 793, 1245, 1561, 1777
SsiI CCGC 2 cut(s) 115, 1741
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 2 cut(s) 294, 1718
StyI CCWWGG 2 cut(s) 214, 319
TaaI ACNGT 1 cut(s) 824
TaiI ACGT 2 cut(s) 919, 1284
TaqI TCGA 3 cut(s) 159, 567, 727
TasI AATT 8 cut(s) 132, 184, 269, 450, 793, 1245, 1561, 1777
TauI GCSGC 1 cut(s) 1744
TfiI GAWTC 5 cut(s) 315, 517, 941, 1082, 1793
Tru1I TTAA 9 cut(s) 86, 135, 272, 773, 861, 1505, 1517, 1581, 1798
Tru9I TTAA 9 cut(s) 86, 135, 272, 773, 861, 1505, 1517, 1581, 1798
TscAI CASTG 4 cut(s) 1366, 1396, 1498, 1534
TseI GCWGC 4 cut(s) 808, 1219, 1634, 1686
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 4 cut(s) 1366, 1396, 1498, 1534
Tth111I GACNNNGTC 1 cut(s) 1328
VneI GTGCAC 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 4 cut(s) 184, 450, 793, 1245
XceI RCATGY 2 cut(s) 199, 497
XmiI GTMKAC 1 cut(s) 431
XmnI GAANNNNTTC 1 cut(s) 824
XspI CTAG 1 cut(s) 537
Zsp2I ATGCAT 3 cut(s) 389, 985, 1668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.