Rh3BG239300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001246
Physical Location & Seq
Forward (+)
22512086 .. 22517226
5141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG239300.1

Sequence Viewer

Length: 1911 bp
ATGCCCAAGGAAAAGGGGTCTGTCTTGGGGGTGTCACGTCAGTCTTCAAGTTCTGGGTTTTGTTCAGAATTTTGGAAAGATGGGAACTTGAACTGGGCGGTGGACTTGTGGGCTGAAGAGAGGTTTTATGGGATGATACTAGAGAGGCTGGACAAGACACTGACATCTCCTAATCTAACAAATAAGGAGGCTCTAAAAGTCCTTGTCAAGGATCAGCTTTTGCGTTCTTCAGAAAATGAAATTGAAGGTTGTAATGCAAATGTGATAGAAGAAAGGACGGCTGAGGTGTCAAATTTGCTTGACATGTTGAGGAGTGTTTCAGTTGGTGATGACGAGCAGTTGAAAACTTGTGATACCCCATCACGTCCTGAATGGAAATTAAAACAGGACAATGAGGAGTTCCGGGTTATGTATCGTGAGGGCATCCAAGGTTCTCCATTTCATACATTACTTGTTGAAGGTTACATAGACGGGCCTGTAGATGTCTGTTTGTGCATCTCATGTGAGGCAGACCTTTACAAGAAATGGTGGCCTCAGTCTACCATTCCAACTTTTAAAATTCTCTATGGGAAGTGTTTACAGAAGGTCGGGATTGGCGAACAGATATCTGTAGTGAGGATGAAGATTCCGTGGCCACTTTCACCTAGGGAAGCTGTTGTGCACTATTTTGTGTTTGAGTACTATGAAGATGACCTGGTTGTTGTTCTCTTGAACACGATCTCTGATTTTGGAGGCATTGATGGTGGCCTTAAAAATGAGGCAACTGATGGAGTAAAGGATGCAGTTAGGATAGATGTAGTTGGTGGCTTTGCTTTGCAGAAGGTTACCGATGATAGAAGTTACTTTCGGACAATAGCAAATATGGATATAAAGATGGATTTTGTCCCACCATCCCTTATAAATTTTGTCTCAAGACAGCTCATTGGCAACGGTTTCAGACTGTATCAAAAGGTAGTGTCTTCTAAGCTTAATCGTGATGAAGATTACAGCAAGGCTTTGAGAGGCCCACTATACTCTCGCATACGTGAAGCTCTTTATTCACTTCATGAATCAAGCCGGCTTCTGGAGGGCAAAAAGTTAAACGGTGATGCATCCAATCTCTCTGAAGAAAATCTAGTTAAACATAAAATGAATGATTTGAAGTTGGTGGACATGAATGAGAAAGTTCATAATGAACACCTTGCAAGTGAAGCTACCCCAGATGATGCACATGTTACTGGAGGAAACACTCTTTGTGAGATTGAGGAGGTAGAGAGTGAAGAAAGCAAGACTGAGGAGATTAAAAGCGAAAAAAGCAAGATTGAGGAGTTAGAGGGCAAAGAAAGCAGGAAGCTTGAGGATCAAACATTCAAAAGAATTCCCGAAAGGGGACATGTAATTGGAAAGAGAAACATGTTCATCAGTTCTGAGGTGGAAGAAGCTCTAGGAACAATAGAAAAAGTCATTTTGAGGGTTAGGCAATATAGGTTCAGCGCTGAAATGCCGTCCTCTGGTTTCACCAATGAAGTCTCACGAAAGGAAAATGATGGAGGTAACCCAGACTCCTTAGAAGATGAAGTCCGCTCAAGTGAAGATTTTTCTGCAGTACCTAAAAATGAGGGCACTGAAGGGGCGTTGACTGCACCTCCAAGGAATAGCAATGCCATTCAAAACATAAGTTATGCAGGCTCAAATTATTTGTCAAAGGAAGTTAACCATAACAGAATAGTACCTACTTCCCCATTGTGTTCCTCCCAAGATGGGATTGTTGAGAGACCTGTTGTGGATAACATCGTGCATAGTACTAACCAAATTAGCTGCAAGACAAATGACACTCATCAAAGTAGTCACAGTAGGAGGAAGAAGTCACGGTGGCAAAGAAGGCGTGGGTTCTGTTGCTTCAGCATCAGTTCAGGACAAATAGATTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

636

Amino Acids

71.78

Weight (kDa)

5.37

Isoelectric Point (pI)

51.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AccBSI CCGCTC 1 cut(s) 1563
AccI GTMKAC 1 cut(s) 539
AciI CCGC 2 cut(s) 98, 1561
AclWI GGATC 2 cut(s) 219, 1347
AcoI YGGCCR 1 cut(s) 632
AcsI RAATTY 5 cut(s) 68, 292, 558, 901, 1356
AcuI CTGAAG 5 cut(s) 135, 213, 1125, 1626, 1864
AfaI GTAC 4 cut(s) 680, 1587, 1710, 1783
AfeI AGCGCT 1 cut(s) 1474
AfiI CCNNNNNNNGG 5 cut(s) 208, 1063, 1367, 1490, 1740
AflIII ACRYGT 4 cut(s) 303, 1210, 1372, 1392
AgsI TTSAA 9 cut(s) 48, 91, 245, 343, 458, 712, 1141, 1351, 1649
AjiI CACGTC 2 cut(s) 38, 365
AjnI CCWGG 1 cut(s) 693
AluBI AGCT 9 cut(s) 217, 653, 919, 967, 1031, 1193, 1333, 1421, 1797
AluI AGCT 9 cut(s) 217, 653, 919, 967, 1031, 1193, 1333, 1421, 1797
Alw21I GWGCWC 1 cut(s) 663
Alw26I GTCTC 3 cut(s) 913, 1513, 1747
Alw44I GTGCAC 1 cut(s) 659
AlwI GGATC 2 cut(s) 219, 1347
Aor51HI AGCGCT 1 cut(s) 1474
AoxI GGCC 5 cut(s) 473, 530, 632, 745, 1003
ApaLI GTGCAC 1 cut(s) 659
ApeKI GCWGC 1 cut(s) 1797
ApoI RAATTY 5 cut(s) 68, 292, 558, 901, 1356
ArsI GACNNNNNNTTYG 2 cut(s) 1664, 1696
AspA2I CCTAGG 1 cut(s) 644
AspLEI GCGC 1 cut(s) 1475
AspS9I GGNCC 2 cut(s) 473, 1004
AsuC2I CCSGG 1 cut(s) 404
AsuHPI GGTGA 4 cut(s) 338, 633, 1097, 1489
AvrII CCTAGG 1 cut(s) 644
BaeGI GKGCMC 2 cut(s) 663, 1604
BalI TGGCCA 1 cut(s) 634
BbsI GAAGAC 2 cut(s) 36, 951
Bbv12I GWGCWC 1 cut(s) 663
BbvCI CCTCAGC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 1784
BccI CCATC 8 cut(s) 74, 367, 734, 761, 868, 898, 1520, 1733
BceAI ACGGC 2 cut(s) 294, 1468
BciT130I CCWGG 1 cut(s) 695
BcnI CCSGG 1 cut(s) 404
BcoDI GTCTC 3 cut(s) 913, 1513, 1747
BfaI CTAG 4 cut(s) 140, 645, 1115, 1424
BfmI CTRYAG 3 cut(s) 477, 609, 1581
BfoI RGCGCY 1 cut(s) 1476
BisI GCNGC 1 cut(s) 1798
BlnI CCTAGG 1 cut(s) 644
BlsI GCNGC 1 cut(s) 1799
BmcAI AGTACT 2 cut(s) 680, 1783
Bme1390I CCNGG 2 cut(s) 404, 695
BmgBI CACGTC 2 cut(s) 38, 365
BmgT120I GGNCC 2 cut(s) 473, 1004
BmrFI CCNGG 2 cut(s) 404, 695
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 7 cut(s) 432, 504, 769, 1078, 1100, 1195, 1893
BmuI ACTGGG 1 cut(s) 103
BpiI GAAGAC 2 cut(s) 36, 951
BpmI CTGGAG 2 cut(s) 1085, 1239
Bpu10I CCTNAGC 1 cut(s) 282
BpuEI CTTGAG 3 cut(s) 895, 1355, 1549
BpuMI CCSGG 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 1025
BsaI GGTCTC 1 cut(s) 1747
BsaJI CCNNGG 5 cut(s) 6, 427, 629, 644, 1628
BsaXI ACNNNNNCTCC 4 cut(s) 1526, 1556, 1609, 1639
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 1063, 1367, 1490, 1740
Bse118I RCCGGY 1 cut(s) 1056
Bse1I ACTGG 2 cut(s) 98, 1222
Bse3DI GCAATG 1 cut(s) 1645
BseBI CCWGG 1 cut(s) 695
BseDI CCNNGG 5 cut(s) 6, 427, 629, 644, 1628
BseGI GGATG 6 cut(s) 138, 423, 624, 784, 890, 1091
BseLI CCNNNNNNNGG 5 cut(s) 208, 1063, 1367, 1490, 1740
BseMI GCAATG 1 cut(s) 1645
BseMII CTCAG 4 cut(s) 273, 548, 1263, 1398
BseNI ACTGG 2 cut(s) 98, 1222
BseRI GAGGAG 5 cut(s) 325, 410, 1259, 1289, 1319
BseSI GKGCMC 2 cut(s) 663, 1604
BseXI GCAGC 1 cut(s) 1784
BsgI GTGCAG 1 cut(s) 1605
BshFI GGCC 5 cut(s) 475, 532, 634, 747, 1005
BsiHKAI GWGCWC 1 cut(s) 663
BsiSI CCGG 2 cut(s) 403, 1057
BslFI GGGAC 2 cut(s) 869, 1383
BslI CCNNNNNNNGG 5 cut(s) 208, 1063, 1367, 1490, 1740
BsmAI GTCTC 3 cut(s) 913, 1513, 1747
BsmFI GGGAC 2 cut(s) 869, 1383
BsnI GGCC 5 cut(s) 475, 532, 634, 747, 1005
Bso31I GGTCTC 1 cut(s) 1747
Bsp1286I GDGCHC 2 cut(s) 663, 1604
Bsp143I GATC 3 cut(s) 211, 717, 1339
BspACI CCGC 2 cut(s) 98, 1561
BspANI GGCC 5 cut(s) 475, 532, 634, 747, 1005
BspCNI CTCAG 4 cut(s) 274, 547, 1264, 1399
BspHI TCATGA 1 cut(s) 1045
BspMAI CTGCAG 1 cut(s) 1585
BspPI GGATC 2 cut(s) 219, 1347
BspTNI GGTCTC 1 cut(s) 1747
BsrBI CCGCTC 1 cut(s) 1563
BsrDI GCAATG 1 cut(s) 1645
BsrFI RCCGGY 1 cut(s) 1056
BsrI ACTGG 2 cut(s) 98, 1222
BssAI RCCGGY 1 cut(s) 1056
BssECI CCNNGG 5 cut(s) 6, 427, 629, 644, 1628
BssMI GATC 3 cut(s) 211, 717, 1339
BssT1I CCWWGG 4 cut(s) 6, 427, 644, 1628
Bst2UI CCWGG 1 cut(s) 695
Bst4CI ACNGT 5 cut(s) 932, 942, 1085, 1832, 1851
Bst6I CTCTTC 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 1058, 1666
BstDEI CTNAG 6 cut(s) 282, 534, 963, 1272, 1407, 1546
BstDSI CCRYGG 1 cut(s) 629
BstEII GGTNACC 2 cut(s) 823, 1532
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 6 cut(s) 138, 423, 624, 784, 890, 1091
BstH2I RGCGCY 1 cut(s) 1476
BstHHI GCGC 1 cut(s) 1475
BstKTI GATC 3 cut(s) 214, 720, 1342
BstMAI GTCTC 3 cut(s) 913, 1513, 1747
BstMBI GATC 3 cut(s) 211, 717, 1339
BstMWI GCNNNNNNNGC 5 cut(s) 1190, 1293, 1323, 1619, 1861
BstNI CCWGG 1 cut(s) 695
BstNSI RCATGY 4 cut(s) 307, 1214, 1376, 1396
BstPI GGTNACC 2 cut(s) 823, 1532
BstSCI CCNGG 2 cut(s) 402, 693
BstSFI CTRYAG 3 cut(s) 477, 609, 1581
BstSLI GKGCMC 2 cut(s) 663, 1604
BstV1I GCAGC 1 cut(s) 1784
BstV2I GAAGAC 2 cut(s) 36, 951
BsuRI GGCC 5 cut(s) 475, 532, 634, 747, 1005
BtgI CCRYGG 1 cut(s) 629
BtrI CACGTC 2 cut(s) 38, 365
BtsCI GGATG 6 cut(s) 138, 423, 624, 784, 890, 1091
BtsIMutI CAGTG 2 cut(s) 158, 1602
Cac8I GCNNGC 2 cut(s) 1058, 1666
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 1 cut(s) 1475
Cfr10I RCCGGY 1 cut(s) 1056
Cfr13I GGNCC 2 cut(s) 473, 1004
CsiI ACCWGGT 1 cut(s) 693
Csp6I GTAC 4 cut(s) 679, 1586, 1709, 1782
CviAII CATG 7 cut(s) 304, 501, 1046, 1153, 1211, 1373, 1393
CviQI GTAC 4 cut(s) 679, 1586, 1709, 1782
DdeI CTNAG 6 cut(s) 282, 534, 963, 1272, 1407, 1546
DpnI GATC 3 cut(s) 213, 719, 1341
DpnII GATC 3 cut(s) 211, 717, 1339
DraI TTTAAA 1 cut(s) 556
EaeI YGGCCR 1 cut(s) 632
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 4 cut(s) 6, 427, 644, 1628
Eco31I GGTCTC 1 cut(s) 1747
Eco32I GATATC 1 cut(s) 606
Eco47III AGCGCT 1 cut(s) 1474
Eco57I CTGAAG 5 cut(s) 135, 213, 1125, 1626, 1864
Eco91I GGTNACC 2 cut(s) 823, 1532
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO65I GGTNACC 2 cut(s) 823, 1532
EcoRI GAATTC 1 cut(s) 1356
EcoRII CCWGG 1 cut(s) 693
EcoRV GATATC 1 cut(s) 606
EcoT14I CCWWGG 4 cut(s) 6, 427, 644, 1628
EcoT22I ATGCAT 1 cut(s) 1093
ErhI CCWWGG 4 cut(s) 6, 427, 644, 1628
FaeI CATG 7 cut(s) 307, 504, 1049, 1156, 1214, 1376, 1396
FaqI GGGAC 2 cut(s) 869, 1383
FatI CATG 7 cut(s) 303, 500, 1045, 1152, 1210, 1372, 1392
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 1 cut(s) 1798
FokI GGATG 6 cut(s) 145, 410, 631, 791, 877, 1078
Fsp4HI GCNGC 1 cut(s) 1798
FspBI CTAG 4 cut(s) 140, 645, 1115, 1424
GlaI GCGC 1 cut(s) 1474
GluI GCNGC 1 cut(s) 1798
GsuI CTGGAG 2 cut(s) 1085, 1239
HaeII RGCGCY 1 cut(s) 1476
HaeIII GGCC 5 cut(s) 475, 532, 634, 747, 1005
HapII CCGG 2 cut(s) 403, 1057
HhaI GCGC 1 cut(s) 1475
Hin1II CATG 7 cut(s) 307, 504, 1049, 1156, 1214, 1376, 1396
Hin6I GCGC 1 cut(s) 1473
HinP1I GCGC 1 cut(s) 1473
HincII GTYRAC 2 cut(s) 1617, 1693
HindII GTYRAC 2 cut(s) 1617, 1693
HindIII AAGCTT 2 cut(s) 965, 1331
HinfI GANTC 4 cut(s) 625, 1049, 1541, 1904
HpaI GTTAAC 1 cut(s) 1693
HpaII CCGG 2 cut(s) 403, 1057
HphI GGTGA 4 cut(s) 338, 633, 1097, 1489
Hpy166II GTNNAC 7 cut(s) 103, 540, 578, 661, 1150, 1617, 1693
Hpy188I TCNGA 7 cut(s) 67, 232, 724, 849, 938, 1105, 1408
Hpy8I GTNNAC 7 cut(s) 103, 540, 578, 661, 1150, 1617, 1693
HpyAV CCTTC 6 cut(s) 239, 452, 577, 814, 1601, 1854
HpyCH4III ACNGT 5 cut(s) 932, 942, 1085, 1832, 1851
HpyCH4IV ACGT 3 cut(s) 37, 364, 1024
HpyF10VI GCNNNNNNNGC 5 cut(s) 1190, 1293, 1323, 1619, 1861
HpyF3I CTNAG 6 cut(s) 282, 534, 963, 1272, 1407, 1546
HpySE526I ACGT 3 cut(s) 37, 364, 1024
Hsp92II CATG 7 cut(s) 307, 504, 1049, 1156, 1214, 1376, 1396
HspAI GCGC 1 cut(s) 1473
KroI GCCGGC 1 cut(s) 1056
KroNI GCCGGC 1 cut(s) 1058
KspAI GTTAAC 1 cut(s) 1693
Kzo9I GATC 3 cut(s) 211, 717, 1339
Lsp1109I GCAGC 1 cut(s) 1784
LweI GCATC 7 cut(s) 432, 504, 769, 1078, 1100, 1195, 1893
MabI ACCWGGT 1 cut(s) 693
MaeI CTAG 4 cut(s) 140, 645, 1115, 1424
MaeII ACGT 3 cut(s) 37, 364, 1024
MaeIII GTNAC 8 cut(s) 33, 461, 823, 839, 1213, 1532, 1826, 1845
MalI GATC 3 cut(s) 213, 719, 1341
MbiI CCGCTC 1 cut(s) 1563
MboI GATC 3 cut(s) 211, 717, 1339
MhlI GDGCHC 2 cut(s) 663, 1604
MlsI TGGCCA 1 cut(s) 634
MluNI TGGCCA 1 cut(s) 634
MlyI GAGTC 1 cut(s) 1535
MmeI TCCRAC 1 cut(s) 572
Mox20I TGGCCA 1 cut(s) 634
Mph1103I ATGCAT 1 cut(s) 1093
MroNI GCCGGC 1 cut(s) 1056
MscI TGGCCA 1 cut(s) 634
MseI TTAA 8 cut(s) 380, 555, 750, 969, 1079, 1119, 1281, 1692
Msp20I TGGCCA 1 cut(s) 634
MspI CCGG 2 cut(s) 403, 1057
MspR9I CCNGG 2 cut(s) 404, 695
MvaI CCWGG 1 cut(s) 695
MwoI GCNNNNNNNGC 5 cut(s) 1190, 1293, 1323, 1619, 1861
NaeI GCCGGC 1 cut(s) 1058
NciI CCSGG 1 cut(s) 404
NdeII GATC 3 cut(s) 211, 717, 1339
NgoMIV GCCGGC 1 cut(s) 1056
NlaIII CATG 7 cut(s) 307, 504, 1049, 1156, 1214, 1376, 1396
NmuCI GTSAC 3 cut(s) 33, 1826, 1845
NsiI ATGCAT 1 cut(s) 1093
NspI RCATGY 4 cut(s) 307, 1214, 1376, 1396
PagI TCATGA 1 cut(s) 1045
PciI ACATGT 4 cut(s) 303, 1210, 1372, 1392
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PdiI GCCGGC 1 cut(s) 1058
PfeI GAWTC 3 cut(s) 625, 1049, 1904
PkrI GCNGC 1 cut(s) 1799
PleI GAGTC 1 cut(s) 1535
PpsI GAGTC 1 cut(s) 1535
Ppu21I YACGTR 1 cut(s) 1025
PscI ACATGT 4 cut(s) 303, 1210, 1372, 1392
PsiI TTATAA 1 cut(s) 899
Psp6I CCWGG 1 cut(s) 693
PspEI GGTNACC 2 cut(s) 823, 1532
PspGI CCWGG 1 cut(s) 693
PspPI GGNCC 2 cut(s) 473, 1004
PstI CTGCAG 1 cut(s) 1585
RsaI GTAC 4 cut(s) 680, 1587, 1710, 1783
RsaNI GTAC 4 cut(s) 679, 1586, 1709, 1782
SaqAI TTAA 8 cut(s) 380, 555, 750, 969, 1079, 1119, 1281, 1692
SatI GCNGC 1 cut(s) 1798
Sau3AI GATC 3 cut(s) 211, 717, 1339
Sau96I GGNCC 2 cut(s) 473, 1004
ScaI AGTACT 2 cut(s) 680, 1783
SchI GAGTC 1 cut(s) 1535
ScrFI CCNGG 2 cut(s) 404, 695
SduI GDGCHC 2 cut(s) 663, 1604
SexAI ACCWGGT 1 cut(s) 693
SfaNI GCATC 7 cut(s) 432, 504, 769, 1078, 1100, 1195, 1893
SfcI CTRYAG 3 cut(s) 477, 609, 1581
SmlI CTYRAG 3 cut(s) 910, 1334, 1564
SmoI CTYRAG 3 cut(s) 910, 1334, 1564
SsiI CCGC 2 cut(s) 98, 1561
SspMI CTAG 4 cut(s) 140, 645, 1115, 1424
StyD4I CCNGG 2 cut(s) 402, 693
StyI CCWWGG 4 cut(s) 6, 427, 644, 1628
TaaI ACNGT 5 cut(s) 932, 942, 1085, 1832, 1851
TaiI ACGT 3 cut(s) 40, 367, 1027
TatI WGTACW 2 cut(s) 678, 1781
TfiI GAWTC 3 cut(s) 625, 1049, 1904
Tru1I TTAA 8 cut(s) 380, 555, 750, 969, 1079, 1119, 1281, 1692
Tru9I TTAA 8 cut(s) 380, 555, 750, 969, 1079, 1119, 1281, 1692
TscAI CASTG 2 cut(s) 165, 1609
TseFI GTSAC 3 cut(s) 33, 1826, 1845
TseI GCWGC 1 cut(s) 1797
Tsp45I GTSAC 3 cut(s) 33, 1826, 1845
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 2 cut(s) 165, 1609
VneI GTGCAC 1 cut(s) 659
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 5 cut(s) 68, 292, 558, 901, 1356
XceI RCATGY 4 cut(s) 307, 1214, 1376, 1396
XmaJI CCTAGG 1 cut(s) 644
XmiI GTMKAC 1 cut(s) 539
XspI CTAG 4 cut(s) 140, 645, 1115, 1424
ZrmI AGTACT 2 cut(s) 680, 1783
Zsp2I ATGCAT 1 cut(s) 1093
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.