Rh3CG233200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001246
Physical Location & Seq
Forward (+)
22015574 .. 22021640
6067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG233200.1

Sequence Viewer

Length: 1803 bp
ATGGAGAAAAAGAGAAAAATCACTCAATACAGAGAGAGGCTGGACAAGACACTGACATCTCCTAATCTAACAAATAAGGAGGCTCTAAAAGTCCTTGTCAAGGATCAGCTTTTGCGATCTTCAGAAAATGAAATTGAAGGTTGTAATGCAAATGTGATAGAAGAAAGGACGGCTGAGGTGTCAAATTTGCTTGACATGTTGAGGAGTGTTTCAGTTGGTGATGATGAGCGGTTGAAAACTTGTGATACCCCATCACGTCCTGAATGGAAATTAAAACAGGACAATGAGGAGTTCCGGGTTATGTATCGTGAGGGCATCCAAGGTTCTCCATTTCATACATTACTTGTTGAAGGTTACGTAGACGGGCCTGTAGATGTCTGTTTGTGCATATCATGTGAGGCAGACCTTTACAAGAAATGGTGGCCTCAGTCTACCATTCCAACTTTTAAAATTCTCTATGGGAAGTGTTTACAGAAGGTCGGGATTGGCGAACAGATATCTGTAGTGAGGATGAAGATTCCGTGGCCACTTTCACCTAGGGAAGCTGTTGTGCACTATTTTGTGTTTGAGTACTATGAAGATGACCTGGTTGTTGTTCTCTTGAACACGATCTCTGATTTTGGAGGCATTGATGGTGGCCTTAAAAATGAGGCAACTGATGGAGTAAAGGATGCAGTTAGGATAGATGTAGTTGGTGGCTTTGCTTTGCAGAAGGTTACTGATGATAGAAGTTACTTTCGGACAATAGCAAATATGGATATAAAGATGGATTTTGTCCCACCATCCCTTATAAATTTTGTCTCAAGACAGCTCATTGGCAACGGTTTCAGGCTGTATCAAAAGGTAGTGTCTTCTAAGCTTAATCGTGATGAAGATTACAGCAAGGCTTTGAGAGGCCCACTATACTCTCGCATACGTGAAGCTCTTTATTCACTTCATGAATCAAGCCGGCTTCTGGAGGGCAAAAAGTTAAACGGTGATGCATCCAATCTCTCTGAAGAAAATCTAGTTAAACATAAAATGAATGATTTGAAGTTGGTGGATATGAATGAGAAAGTTCATAATGAACACCTTGCAAGTGAAGCTACCCCAGATGATGCACATGTTACTGGAGGAAACACTCTTTGTGAGATTGAGGAGGTAGAGAGTGAAGAAAGCAAGACTGAGGAGATTAAAAATGAAAAAAGCAAGATTGAGGAGTTAGAGGGCAAAGAAAGCAGGCAGCTTGAGGATCAAACATTCAAAAGAATTCCCGAAAGGGGACATGTAATTGGAAAGAGAAACATTTTCATCAGTTCTGAGGTGGAAGAAGCTCTAGGAACAATAGAAAAAGTCATTTTGAGGGTTAGGCAATATAGGTTGAGTGCTGAAATGCCATCCTCTGGTTTCACCAATGAAGTCTCACGAAAGGAAAATGATGGAGGCAACCCAGAGTCCTTAGAAGATGAAGTCCGCTCAAGTGAACATTTTTCTGCAGTACCTAAAAATGAGGGCACTGAAGGGGCGTTGACTGCACCTCCAAGGAATAGCAATGCCATTCAAAACATAAGTTATGCAGGCTCAAATTATTTGTCAAAGGAAGTTAACCATAACAGAATAGTACCTACTTCCCCATTGTGTTCCTCCCAAGATGGGATTGTTGAGATACCTGTTGTGGATAACATCGTGCATAGTACTAACCAAATTAGCTGCAAGACAAATGACACTCATCAAAGTAGTCACAGTAGAAGGAAGAAGTCACGGTGGCAAAGAAGGCGTGGGTTCTGTTGCTTCAGCATCAGTTCAGGACAAATAGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

600

Amino Acids

67.93

Weight (kDa)

5.66

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
AccB7I CCANNNNNTGG 1 cut(s) 1382
AccBSI CCGCTC 2 cut(s) 229, 1455
AccI GTMKAC 2 cut(s) 360, 431
AciI CCGC 2 cut(s) 229, 1453
AclWI GGATC 2 cut(s) 111, 1239
AcoI YGGCCR 1 cut(s) 524
AcsI RAATTY 4 cut(s) 184, 450, 793, 1248
AcuI CTGAAG 4 cut(s) 105, 1017, 1518, 1756
AfaI GTAC 4 cut(s) 572, 1479, 1602, 1675
AfiI CCNNNNNNNGG 5 cut(s) 100, 955, 1259, 1382, 1632
AflIII ACRYGT 3 cut(s) 195, 1102, 1264
AgsI TTSAA 7 cut(s) 137, 235, 350, 604, 1033, 1243, 1541
AjiI CACGTC 1 cut(s) 257
AjnI CCWGG 1 cut(s) 585
AluBI AGCT 9 cut(s) 109, 545, 811, 859, 923, 1085, 1225, 1313, 1689
AluI AGCT 9 cut(s) 109, 545, 811, 859, 923, 1085, 1225, 1313, 1689
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 2 cut(s) 805, 1405
Alw44I GTGCAC 1 cut(s) 551
AlwI GGATC 2 cut(s) 111, 1239
AoxI GGCC 5 cut(s) 365, 422, 524, 637, 895
ApaLI GTGCAC 1 cut(s) 551
ApeKI GCWGC 2 cut(s) 1222, 1689
ApoI RAATTY 4 cut(s) 184, 450, 793, 1248
ArsI GACNNNNNNTTYG 2 cut(s) 1556, 1588
AspA2I CCTAGG 1 cut(s) 536
AspS9I GGNCC 2 cut(s) 365, 896
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 4 cut(s) 230, 525, 989, 1381
AvrII CCTAGG 1 cut(s) 536
BaeGI GKGCMC 2 cut(s) 555, 1496
BalI TGGCCA 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 555
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 1234, 1676
BccI CCATC 8 cut(s) 259, 626, 653, 760, 790, 1384, 1412, 1625
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 587
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 805, 1405
BfaI CTAG 3 cut(s) 537, 1007, 1316
BfmI CTRYAG 3 cut(s) 369, 501, 1473
BisI GCNGC 2 cut(s) 1223, 1690
BlnI CCTAGG 1 cut(s) 536
BlsI GCNGC 2 cut(s) 1224, 1691
BmcAI AGTACT 2 cut(s) 572, 1675
Bme1390I CCNGG 2 cut(s) 296, 587
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 365, 896
BmrFI CCNGG 2 cut(s) 296, 587
BmsI GCATC 6 cut(s) 324, 661, 970, 992, 1087, 1785
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 2 cut(s) 977, 1131
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 3 cut(s) 787, 1247, 1441
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 2 cut(s) 358, 917
BsaJI CCNNGG 4 cut(s) 319, 521, 536, 1520
BsaXI ACNNNNNCTCC 2 cut(s) 1501, 1531
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 955, 1259, 1382, 1632
Bse118I RCCGGY 1 cut(s) 948
Bse1I ACTGG 1 cut(s) 1114
Bse3DI GCAATG 1 cut(s) 1537
BseBI CCWGG 1 cut(s) 587
BseDI CCNNGG 4 cut(s) 319, 521, 536, 1520
BseGI GGATG 6 cut(s) 315, 516, 676, 782, 983, 1376
BseLI CCNNNNNNNGG 5 cut(s) 100, 955, 1259, 1382, 1632
BseMI GCAATG 1 cut(s) 1537
BseMII CTCAG 4 cut(s) 165, 440, 1155, 1290
BseNI ACTGG 1 cut(s) 1114
BseRI GAGGAG 5 cut(s) 217, 302, 1151, 1181, 1211
BseSI GKGCMC 2 cut(s) 555, 1496
BseXI GCAGC 2 cut(s) 1234, 1676
BsgI GTGCAG 1 cut(s) 1497
BshFI GGCC 5 cut(s) 367, 424, 526, 639, 897
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 295, 949
BslFI GGGAC 2 cut(s) 761, 1275
BslI CCNNNNNNNGG 5 cut(s) 100, 955, 1259, 1382, 1632
BsmAI GTCTC 2 cut(s) 805, 1405
BsmFI GGGAC 2 cut(s) 761, 1275
BsnI GGCC 5 cut(s) 367, 424, 526, 639, 897
Bsp1286I GDGCHC 2 cut(s) 555, 1496
Bsp143I GATC 4 cut(s) 103, 116, 609, 1231
BspACI CCGC 2 cut(s) 229, 1453
BspANI GGCC 5 cut(s) 367, 424, 526, 639, 897
BspCNI CTCAG 4 cut(s) 166, 439, 1156, 1291
BspHI TCATGA 1 cut(s) 937
BspMAI CTGCAG 1 cut(s) 1477
BspPI GGATC 2 cut(s) 111, 1239
BsrBI CCGCTC 2 cut(s) 229, 1455
BsrDI GCAATG 1 cut(s) 1537
BsrFI RCCGGY 1 cut(s) 948
BsrI ACTGG 1 cut(s) 1114
BssAI RCCGGY 1 cut(s) 948
BssECI CCNNGG 4 cut(s) 319, 521, 536, 1520
BssMI GATC 4 cut(s) 103, 116, 609, 1231
BssT1I CCWWGG 3 cut(s) 319, 536, 1520
Bst2UI CCWGG 1 cut(s) 587
Bst4CI ACNGT 4 cut(s) 824, 977, 1724, 1743
BstBAI YACGTR 2 cut(s) 358, 917
BstC8I GCNNGC 3 cut(s) 950, 1220, 1558
BstDEI CTNAG 6 cut(s) 174, 426, 855, 1164, 1299, 1438
BstDSI CCRYGG 1 cut(s) 521
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 6 cut(s) 315, 516, 676, 782, 983, 1376
BstKTI GATC 4 cut(s) 106, 119, 612, 1234
BstMAI GTCTC 2 cut(s) 805, 1405
BstMBI GATC 4 cut(s) 103, 116, 609, 1231
BstMWI GCNNNNNNNGC 4 cut(s) 1082, 1215, 1511, 1753
BstNI CCWGG 1 cut(s) 587
BstNSI RCATGY 3 cut(s) 199, 1106, 1268
BstSCI CCNGG 2 cut(s) 294, 585
BstSFI CTRYAG 3 cut(s) 369, 501, 1473
BstSLI GKGCMC 2 cut(s) 555, 1496
BstSNI TACGTA 1 cut(s) 358
BstV1I GCAGC 2 cut(s) 1234, 1676
BstV2I GAAGAC 1 cut(s) 843
BsuRI GGCC 5 cut(s) 367, 424, 526, 639, 897
BtgI CCRYGG 1 cut(s) 521
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 6 cut(s) 315, 516, 676, 782, 983, 1376
BtsIMutI CAGTG 2 cut(s) 50, 1494
Cac8I GCNNGC 3 cut(s) 950, 1220, 1558
CciI TCATGA 1 cut(s) 937
Cfr10I RCCGGY 1 cut(s) 948
Cfr13I GGNCC 2 cut(s) 365, 896
CsiI ACCWGGT 1 cut(s) 585
Csp6I GTAC 4 cut(s) 571, 1478, 1601, 1674
CviAII CATG 5 cut(s) 196, 393, 938, 1103, 1265
CviQI GTAC 4 cut(s) 571, 1478, 1601, 1674
DdeI CTNAG 6 cut(s) 174, 426, 855, 1164, 1299, 1438
DpnI GATC 4 cut(s) 105, 118, 611, 1233
DpnII GATC 4 cut(s) 103, 116, 609, 1231
DraI TTTAAA 1 cut(s) 448
EaeI YGGCCR 1 cut(s) 524
Eco105I TACGTA 1 cut(s) 358
Eco130I CCWWGG 3 cut(s) 319, 536, 1520
Eco32I GATATC 1 cut(s) 498
Eco57I CTGAAG 4 cut(s) 105, 1017, 1518, 1756
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 1248
EcoRII CCWGG 1 cut(s) 585
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 3 cut(s) 319, 536, 1520
EcoT22I ATGCAT 1 cut(s) 985
ErhI CCWWGG 3 cut(s) 319, 536, 1520
FaeI CATG 5 cut(s) 199, 396, 941, 1106, 1268
FaqI GGGAC 2 cut(s) 761, 1275
FatI CATG 5 cut(s) 195, 392, 937, 1102, 1264
FblI GTMKAC 2 cut(s) 360, 431
Fnu4HI GCNGC 2 cut(s) 1223, 1690
FokI GGATG 6 cut(s) 302, 523, 683, 769, 970, 1363
Fsp4HI GCNGC 2 cut(s) 1223, 1690
FspBI CTAG 3 cut(s) 537, 1007, 1316
GluI GCNGC 2 cut(s) 1223, 1690
GsuI CTGGAG 2 cut(s) 977, 1131
HaeIII GGCC 5 cut(s) 367, 424, 526, 639, 897
HapII CCGG 2 cut(s) 295, 949
Hin1II CATG 5 cut(s) 199, 396, 941, 1106, 1268
HincII GTYRAC 2 cut(s) 1509, 1585
HindII GTYRAC 2 cut(s) 1509, 1585
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 3 cut(s) 517, 941, 1433
HpaI GTTAAC 1 cut(s) 1585
HpaII CCGG 2 cut(s) 295, 949
HphI GGTGA 4 cut(s) 230, 525, 989, 1381
Hpy166II GTNNAC 7 cut(s) 361, 432, 470, 553, 1463, 1509, 1585
Hpy188I TCNGA 5 cut(s) 124, 616, 741, 997, 1300
Hpy8I GTNNAC 7 cut(s) 361, 432, 470, 553, 1463, 1509, 1585
HpyAV CCTTC 7 cut(s) 131, 344, 469, 706, 1493, 1722, 1746
HpyCH4III ACNGT 4 cut(s) 824, 977, 1724, 1743
HpyCH4IV ACGT 3 cut(s) 256, 357, 916
HpyF10VI GCNNNNNNNGC 4 cut(s) 1082, 1215, 1511, 1753
HpyF3I CTNAG 6 cut(s) 174, 426, 855, 1164, 1299, 1438
HpySE526I ACGT 3 cut(s) 256, 357, 916
Hsp92II CATG 5 cut(s) 199, 396, 941, 1106, 1268
KroI GCCGGC 1 cut(s) 948
KroNI GCCGGC 1 cut(s) 950
KspAI GTTAAC 1 cut(s) 1585
Kzo9I GATC 4 cut(s) 103, 116, 609, 1231
Lsp1109I GCAGC 2 cut(s) 1234, 1676
LweI GCATC 6 cut(s) 324, 661, 970, 992, 1087, 1785
MabI ACCWGGT 1 cut(s) 585
MaeI CTAG 3 cut(s) 537, 1007, 1316
MaeII ACGT 3 cut(s) 256, 357, 916
MaeIII GTNAC 6 cut(s) 353, 715, 731, 1105, 1718, 1737
MalI GATC 4 cut(s) 105, 118, 611, 1233
MbiI CCGCTC 2 cut(s) 229, 1455
MboI GATC 4 cut(s) 103, 116, 609, 1231
MhlI GDGCHC 2 cut(s) 555, 1496
MlsI TGGCCA 1 cut(s) 526
MluCI AATT 9 cut(s) 132, 184, 269, 450, 793, 1248, 1269, 1564, 1683
MluNI TGGCCA 1 cut(s) 526
MlyI GAGTC 1 cut(s) 1442
MmeI TCCRAC 1 cut(s) 464
Mox20I TGGCCA 1 cut(s) 526
Mph1103I ATGCAT 1 cut(s) 985
MroNI GCCGGC 1 cut(s) 948
MscI TGGCCA 1 cut(s) 526
MseI TTAA 8 cut(s) 272, 447, 642, 861, 971, 1011, 1173, 1584
Msp20I TGGCCA 1 cut(s) 526
MspI CCGG 2 cut(s) 295, 949
MspR9I CCNGG 2 cut(s) 296, 587
MvaI CCWGG 1 cut(s) 587
MwoI GCNNNNNNNGC 4 cut(s) 1082, 1215, 1511, 1753
NaeI GCCGGC 1 cut(s) 950
NciI CCSGG 1 cut(s) 296
NdeII GATC 4 cut(s) 103, 116, 609, 1231
NgoMIV GCCGGC 1 cut(s) 948
NlaIII CATG 5 cut(s) 199, 396, 941, 1106, 1268
NmuCI GTSAC 2 cut(s) 1718, 1737
NsiI ATGCAT 1 cut(s) 985
NspI RCATGY 3 cut(s) 199, 1106, 1268
PagI TCATGA 1 cut(s) 937
PciI ACATGT 3 cut(s) 195, 1102, 1264
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PdiI GCCGGC 1 cut(s) 950
PfeI GAWTC 2 cut(s) 517, 941
PflMI CCANNNNNTGG 1 cut(s) 1382
PkrI GCNGC 2 cut(s) 1224, 1691
PleI GAGTC 1 cut(s) 1441
PpsI GAGTC 1 cut(s) 1441
Ppu21I YACGTR 2 cut(s) 358, 917
PscI ACATGT 3 cut(s) 195, 1102, 1264
PsiI TTATAA 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 585
PspGI CCWGG 1 cut(s) 585
PspPI GGNCC 2 cut(s) 365, 896
PstI CTGCAG 1 cut(s) 1477
RsaI GTAC 4 cut(s) 572, 1479, 1602, 1675
RsaNI GTAC 4 cut(s) 571, 1478, 1601, 1674
SaqAI TTAA 8 cut(s) 272, 447, 642, 861, 971, 1011, 1173, 1584
SatI GCNGC 2 cut(s) 1223, 1690
Sau3AI GATC 4 cut(s) 103, 116, 609, 1231
Sau96I GGNCC 2 cut(s) 365, 896
ScaI AGTACT 2 cut(s) 572, 1675
SchI GAGTC 1 cut(s) 1442
ScrFI CCNGG 2 cut(s) 296, 587
SduI GDGCHC 2 cut(s) 555, 1496
SexAI ACCWGGT 1 cut(s) 585
SfaNI GCATC 6 cut(s) 324, 661, 970, 992, 1087, 1785
SfcI CTRYAG 3 cut(s) 369, 501, 1473
SmlI CTYRAG 3 cut(s) 802, 1226, 1456
SmoI CTYRAG 3 cut(s) 802, 1226, 1456
SnaBI TACGTA 1 cut(s) 358
Sse9I AATT 9 cut(s) 132, 184, 269, 450, 793, 1248, 1269, 1564, 1683
SsiI CCGC 2 cut(s) 229, 1453
SspMI CTAG 3 cut(s) 537, 1007, 1316
StyD4I CCNGG 2 cut(s) 294, 585
StyI CCWWGG 3 cut(s) 319, 536, 1520
TaaI ACNGT 4 cut(s) 824, 977, 1724, 1743
TaiI ACGT 3 cut(s) 259, 360, 919
TasI AATT 9 cut(s) 132, 184, 269, 450, 793, 1248, 1269, 1564, 1683
TatI WGTACW 2 cut(s) 570, 1673
TfiI GAWTC 2 cut(s) 517, 941
Tru1I TTAA 8 cut(s) 272, 447, 642, 861, 971, 1011, 1173, 1584
Tru9I TTAA 8 cut(s) 272, 447, 642, 861, 971, 1011, 1173, 1584
TscAI CASTG 2 cut(s) 57, 1501
TseFI GTSAC 2 cut(s) 1718, 1737
TseI GCWGC 2 cut(s) 1222, 1689
Tsp45I GTSAC 2 cut(s) 1718, 1737
TspGWI ACGGA 1 cut(s) 510
TspRI CASTG 2 cut(s) 57, 1501
Van91I CCANNNNNTGG 1 cut(s) 1382
VneI GTGCAC 1 cut(s) 551
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 4 cut(s) 184, 450, 793, 1248
XceI RCATGY 3 cut(s) 199, 1106, 1268
XmaJI CCTAGG 1 cut(s) 536
XmiI GTMKAC 2 cut(s) 360, 431
XspI CTAG 3 cut(s) 537, 1007, 1316
ZrmI AGTACT 2 cut(s) 572, 1675
Zsp2I ATGCAT 1 cut(s) 985
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.