Rroxscaffold_6G00405100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001246
Physical Location & Seq
Reverse (-)
27553915 .. 27559476
5562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405100.1

Sequence Viewer

Length: 1476 bp
ATGGAGAAAAAGAGAAAAATCACTCAATACAGAGAGAGGCTGGACAAGACACTGACATCTCCTAACCTAACAAATAAGGAGGCTCTAAAAGTCCTTGTCAAGGATCAGCTTTTGCGTTCTTCAGAAAATGAAATTGAAGGTTGTAATGCAAATGTGATAGAAGAAAGGACGGCTGAGGTGTCAAATCTGCTTGACATGTTGAGGAGTGTTTCAGTTGGTGATGACAAGGGGTTGAAAACTTGTGATACCCCATCACGTCCTGAATGGAAATTAAAACAGGACAATGAGGAGTTCCGGGTTATGTATCGTGAGGGAATCCAAGGTTCTCCATTTCATACATTGCTTGTTGAAGGTTACATAGATGGGCCTGTAGATATCTCTGATTTTGGAGGCATTGATGGTGGCCTTAAAAATGAGGCAACTGATGGAGTGAAGGATGCAGTTAGGATAGATGTAGTTGGTGGTTTTGCTTTGCAGAAGGTTACCGATGATAGAAGTTACTTTCGGTATGAAATGGTAGTGTCTTCTAAGCTTAATCGTGATGAAGATTACAGCAAGGCTTTGAGAGGCCCACTATACTCTTGCATACGTGAAGCTCTTTATTCACTTCATGAATCAAGCCGGCTTCCGGAGGGAAAAAAGTTAAATGGTGATGCATCCAATCTCTCTGAAGAAAATCTAGTTAAACATAAAATGAATGATTTGAAGTTGGTGGATATGAATGAGAAAGTTCATAATGAACACCTTGCAAGTGAAGCTACCCCAGATGATGCACATGTTACCGGAGGAAACACTCTTTGTGAGATTGAGGAGGTAGAGAGTGAAGAAAGCAAGACTGAGGAGATTAAAAGCGAAAAAAGCACGATTGAGGAGGTAGGGGGCAAAGAAAGCAGGCAGCTTGAGGATCAAACATTCAAAAGAATTCCCGAAAGGGGACATGTAATTGGAAAGAGAAACATGTTCATCAGTTCCGAGGTGGAACAAGCTATAGGAACAATAGATAAAGTCATTTTGAGGGTTAGGCAATATAGGTTCAGCGCTGAAATGCCATCCTCTGGTTTCACCAATGAAGTCTCACGAAAGGAAAATGATGGAGGTCACCCAGAGTCCATTGAAGATGAAGTCCGCTCAAGTGAACGTTTTTCTGCAGTATCTAAAAATGAGGGCACTGAAGGGACGTTGACTACACCTCCAAGGAATAGCAATGCCATTCAAAACATAAGTTATGCAGGCTCAAATTCTTTGTCAAAGGAAGTTAACCATAACAGAATAGTACCTACTTCCCCATTGTGTTCCTCCAAAGATGGGATTGTTGTGATACCTGTTGTGGATAACATTGTGCATAGTACTAACCAAATTAGCTGCAAGACAAATGACACTTATCAAAGTAGTCATGGTAGAAGGAAGAAGTCAAGGTGGCAAAGAAGGCGTGGGTTCTGTTGCTTCAGCATCAATTCAGGACAAATAGATTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

491

Amino Acids

55.07

Weight (kDa)

5.6

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1055
AccBSI CCGCTC 1 cut(s) 1128
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 1 cut(s) 1126
AclI AACGTT 1 cut(s) 1138
AclWI GGATC 2 cut(s) 111, 912
AcsI RAATTY 2 cut(s) 921, 1237
AcuI CTGAAG 4 cut(s) 105, 690, 1191, 1429
AfaI GTAC 2 cut(s) 1275, 1348
AfeI AGCGCT 1 cut(s) 1039
AfiI CCNNNNNNNGG 5 cut(s) 100, 628, 932, 1055, 1305
AflIII ACRYGT 4 cut(s) 195, 775, 937, 957
AgsI TTSAA 7 cut(s) 137, 235, 350, 706, 916, 1115, 1214
AjiI CACGTC 1 cut(s) 257
AluBI AGCT 7 cut(s) 109, 532, 596, 758, 898, 986, 1362
AluI AGCT 7 cut(s) 109, 532, 596, 758, 898, 986, 1362
Alw26I GTCTC 1 cut(s) 1078
AlwI GGATC 2 cut(s) 111, 912
Aor13HI TCCGGA 1 cut(s) 628
Aor51HI AGCGCT 1 cut(s) 1039
AoxI GGCC 3 cut(s) 365, 403, 568
ApeKI GCWGC 2 cut(s) 895, 1362
ApoI RAATTY 2 cut(s) 921, 1237
ArsI GACNNNNNNTTYG 2 cut(s) 1229, 1261
AspLEI GCGC 1 cut(s) 1040
AspS9I GGNCC 2 cut(s) 365, 569
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 4 cut(s) 230, 662, 1054, 1091
BaeGI GKGCMC 1 cut(s) 1169
BbsI GAAGAC 1 cut(s) 516
BbvCI CCTCAGC 1 cut(s) 174
BbvI GCAGC 2 cut(s) 907, 1349
BccI CCATC 7 cut(s) 259, 356, 392, 419, 1057, 1085, 1298
BceAI ACGGC 1 cut(s) 186
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 1078
BfaI CTAG 1 cut(s) 680
BfmI CTRYAG 3 cut(s) 369, 987, 1146
BfoI RGCGCY 1 cut(s) 1041
BisI GCNGC 2 cut(s) 896, 1363
BlsI GCNGC 2 cut(s) 897, 1364
BmcAI AGTACT 1 cut(s) 1348
Bme1390I CCNGG 1 cut(s) 296
BmgBI CACGTC 1 cut(s) 257
BmgT120I GGNCC 2 cut(s) 365, 569
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 5 cut(s) 427, 643, 665, 760, 1458
BpiI GAAGAC 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 2 cut(s) 920, 1114
BpuMI CCSGG 1 cut(s) 296
BsaAI YACGTR 1 cut(s) 590
BsaJI CCNNGG 3 cut(s) 319, 972, 1193
BsaWI WCCGGW 2 cut(s) 628, 782
BsaXI ACNNNNNCTCC 2 cut(s) 1174, 1204
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 628, 932, 1055, 1305
Bse118I RCCGGY 1 cut(s) 621
Bse3DI GCAATG 2 cut(s) 338, 1210
BseAI TCCGGA 1 cut(s) 628
BseDI CCNNGG 3 cut(s) 319, 972, 1193
BseGI GGATG 3 cut(s) 442, 656, 1049
BseLI CCNNNNNNNGG 5 cut(s) 100, 628, 932, 1055, 1305
BseMI GCAATG 2 cut(s) 338, 1210
BseMII CTCAG 2 cut(s) 165, 828
BseRI GAGGAG 5 cut(s) 217, 302, 824, 854, 884
BseSI GKGCMC 1 cut(s) 1169
BseXI GCAGC 2 cut(s) 907, 1349
BshFI GGCC 3 cut(s) 367, 405, 570
BsiSI CCGG 4 cut(s) 295, 622, 629, 783
BslFI GGGAC 2 cut(s) 948, 1189
BslI CCNNNNNNNGG 5 cut(s) 100, 628, 932, 1055, 1305
BsmAI GTCTC 1 cut(s) 1078
BsmFI GGGAC 2 cut(s) 948, 1189
BsnI GGCC 3 cut(s) 367, 405, 570
Bsp1286I GDGCHC 1 cut(s) 1169
Bsp13I TCCGGA 1 cut(s) 628
Bsp143I GATC 2 cut(s) 103, 904
BspACI CCGC 1 cut(s) 1126
BspANI GGCC 3 cut(s) 367, 405, 570
BspCNI CTCAG 2 cut(s) 166, 829
BspEI TCCGGA 1 cut(s) 628
BspHI TCATGA 1 cut(s) 610
BspMAI CTGCAG 1 cut(s) 1150
BspPI GGATC 2 cut(s) 111, 912
BsrBI CCGCTC 1 cut(s) 1128
BsrDI GCAATG 2 cut(s) 338, 1210
BsrFI RCCGGY 1 cut(s) 621
BssAI RCCGGY 1 cut(s) 621
BssECI CCNNGG 3 cut(s) 319, 972, 1193
BssMI GATC 2 cut(s) 103, 904
BssT1I CCWWGG 2 cut(s) 319, 1193
BstBAI YACGTR 1 cut(s) 590
BstC8I GCNNGC 3 cut(s) 623, 893, 1231
BstDEI CTNAG 3 cut(s) 174, 528, 837
BstEII GGTNACC 2 cut(s) 481, 1097
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 3 cut(s) 442, 656, 1049
BstH2I RGCGCY 1 cut(s) 1041
BstHHI GCGC 1 cut(s) 1040
BstKTI GATC 2 cut(s) 106, 907
BstMAI GTCTC 1 cut(s) 1078
BstMBI GATC 2 cut(s) 103, 904
BstMWI GCNNNNNNNGC 4 cut(s) 755, 858, 888, 1426
BstNSI RCATGY 4 cut(s) 199, 779, 941, 961
BstPI GGTNACC 2 cut(s) 481, 1097
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 3 cut(s) 369, 987, 1146
BstSLI GKGCMC 1 cut(s) 1169
BstV1I GCAGC 2 cut(s) 907, 1349
BstV2I GAAGAC 1 cut(s) 516
BsuRI GGCC 3 cut(s) 367, 405, 570
BtrI CACGTC 1 cut(s) 257
BtsCI GGATG 3 cut(s) 442, 656, 1049
BtsIMutI CAGTG 2 cut(s) 50, 1167
Cac8I GCNNGC 3 cut(s) 623, 893, 1231
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 1040
Cfr10I RCCGGY 1 cut(s) 621
Cfr13I GGNCC 2 cut(s) 365, 569
Csp6I GTAC 2 cut(s) 1274, 1347
CviAII CATG 6 cut(s) 196, 611, 776, 938, 958, 1394
CviQI GTAC 2 cut(s) 1274, 1347
DdeI CTNAG 3 cut(s) 174, 528, 837
DpnI GATC 2 cut(s) 105, 906
DpnII GATC 2 cut(s) 103, 904
Eco130I CCWWGG 2 cut(s) 319, 1193
Eco32I GATATC 1 cut(s) 376
Eco47III AGCGCT 1 cut(s) 1039
Eco57I CTGAAG 4 cut(s) 105, 690, 1191, 1429
Eco91I GGTNACC 2 cut(s) 481, 1097
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoO65I GGTNACC 2 cut(s) 481, 1097
EcoRI GAATTC 1 cut(s) 921
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 319, 1193
EcoT22I ATGCAT 1 cut(s) 658
ErhI CCWWGG 2 cut(s) 319, 1193
FaeI CATG 6 cut(s) 199, 614, 779, 941, 961, 1397
FaqI GGGAC 2 cut(s) 948, 1189
FatI CATG 6 cut(s) 195, 610, 775, 937, 957, 1393
Fnu4HI GCNGC 2 cut(s) 896, 1363
FokI GGATG 3 cut(s) 449, 643, 1036
Fsp4HI GCNGC 2 cut(s) 896, 1363
FspBI CTAG 1 cut(s) 680
GlaI GCGC 1 cut(s) 1039
GluI GCNGC 2 cut(s) 896, 1363
HaeII RGCGCY 1 cut(s) 1041
HaeIII GGCC 3 cut(s) 367, 405, 570
HapII CCGG 4 cut(s) 295, 622, 629, 783
HhaI GCGC 1 cut(s) 1040
Hin1II CATG 6 cut(s) 199, 614, 779, 941, 961, 1397
Hin6I GCGC 1 cut(s) 1038
HinP1I GCGC 1 cut(s) 1038
HincII GTYRAC 2 cut(s) 1182, 1258
HindII GTYRAC 2 cut(s) 1182, 1258
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 4 cut(s) 315, 614, 1106, 1469
HpaI GTTAAC 1 cut(s) 1258
HpaII CCGG 4 cut(s) 295, 622, 629, 783
HphI GGTGA 4 cut(s) 230, 662, 1054, 1091
Hpy166II GTNNAC 3 cut(s) 1136, 1182, 1258
Hpy188I TCNGA 4 cut(s) 124, 382, 670, 973
Hpy188III TCNNGA 8 cut(s) 260, 308, 539, 611, 629, 926, 1077, 1458
Hpy8I GTNNAC 3 cut(s) 1136, 1182, 1258
HpyAV CCTTC 7 cut(s) 131, 344, 427, 472, 1166, 1395, 1419
HpyCH4IV ACGT 4 cut(s) 256, 589, 1138, 1178
HpyF10VI GCNNNNNNNGC 4 cut(s) 755, 858, 888, 1426
HpyF3I CTNAG 3 cut(s) 174, 528, 837
HpySE526I ACGT 4 cut(s) 256, 589, 1138, 1178
Hsp92II CATG 6 cut(s) 199, 614, 779, 941, 961, 1397
HspAI GCGC 1 cut(s) 1038
Kpn2I TCCGGA 1 cut(s) 628
KroI GCCGGC 1 cut(s) 621
KroNI GCCGGC 1 cut(s) 623
KspAI GTTAAC 1 cut(s) 1258
Kzo9I GATC 2 cut(s) 103, 904
Lsp1109I GCAGC 2 cut(s) 907, 1349
LweI GCATC 5 cut(s) 427, 643, 665, 760, 1458
MaeI CTAG 1 cut(s) 680
MaeII ACGT 4 cut(s) 256, 589, 1138, 1178
MaeIII GTNAC 5 cut(s) 353, 481, 497, 778, 1097
MalI GATC 2 cut(s) 105, 906
MbiI CCGCTC 1 cut(s) 1128
MboI GATC 2 cut(s) 103, 904
MboII GAAGA 8 cut(s) 111, 173, 516, 557, 683, 836, 1127, 1417
MhlI GDGCHC 1 cut(s) 1169
MluCI AATT 7 cut(s) 132, 269, 921, 942, 1237, 1356, 1453
MlyI GAGTC 1 cut(s) 1115
Mph1103I ATGCAT 1 cut(s) 658
MroI TCCGGA 1 cut(s) 628
MroNI GCCGGC 1 cut(s) 621
MseI TTAA 7 cut(s) 272, 408, 534, 644, 684, 846, 1257
MspI CCGG 4 cut(s) 295, 622, 629, 783
MspR9I CCNGG 1 cut(s) 296
MwoI GCNNNNNNNGC 4 cut(s) 755, 858, 888, 1426
NaeI GCCGGC 1 cut(s) 623
NciI CCSGG 1 cut(s) 296
NdeII GATC 2 cut(s) 103, 904
NgoMIV GCCGGC 1 cut(s) 621
NlaIII CATG 6 cut(s) 199, 614, 779, 941, 961, 1397
NmuCI GTSAC 1 cut(s) 1097
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 4 cut(s) 199, 779, 941, 961
PagI TCATGA 1 cut(s) 610
PciI ACATGT 4 cut(s) 195, 775, 937, 957
PdiI GCCGGC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 315, 614, 1469
PflMI CCANNNNNTGG 1 cut(s) 1055
PkrI GCNGC 2 cut(s) 897, 1364
PleI GAGTC 1 cut(s) 1114
PpsI GAGTC 1 cut(s) 1114
Ppu21I YACGTR 1 cut(s) 590
PscI ACATGT 4 cut(s) 195, 775, 937, 957
Psp1406I AACGTT 1 cut(s) 1138
PspEI GGTNACC 2 cut(s) 481, 1097
PspPI GGNCC 2 cut(s) 365, 569
PstI CTGCAG 1 cut(s) 1150
RsaI GTAC 2 cut(s) 1275, 1348
RsaNI GTAC 2 cut(s) 1274, 1347
SaqAI TTAA 7 cut(s) 272, 408, 534, 644, 684, 846, 1257
SatI GCNGC 2 cut(s) 896, 1363
Sau3AI GATC 2 cut(s) 103, 904
Sau96I GGNCC 2 cut(s) 365, 569
ScaI AGTACT 1 cut(s) 1348
SchI GAGTC 1 cut(s) 1115
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 1 cut(s) 1169
SfaNI GCATC 5 cut(s) 427, 643, 665, 760, 1458
SfcI CTRYAG 3 cut(s) 369, 987, 1146
SmlI CTYRAG 2 cut(s) 899, 1129
SmoI CTYRAG 2 cut(s) 899, 1129
Sse9I AATT 7 cut(s) 132, 269, 921, 942, 1237, 1356, 1453
SsiI CCGC 1 cut(s) 1126
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 2 cut(s) 319, 1193
TaiI ACGT 4 cut(s) 259, 592, 1141, 1181
TasI AATT 7 cut(s) 132, 269, 921, 942, 1237, 1356, 1453
TatI WGTACW 1 cut(s) 1346
TfiI GAWTC 3 cut(s) 315, 614, 1469
Tru1I TTAA 7 cut(s) 272, 408, 534, 644, 684, 846, 1257
Tru9I TTAA 7 cut(s) 272, 408, 534, 644, 684, 846, 1257
TscAI CASTG 2 cut(s) 57, 1174
TseFI GTSAC 1 cut(s) 1097
TseI GCWGC 2 cut(s) 895, 1362
Tsp45I GTSAC 1 cut(s) 1097
TspRI CASTG 2 cut(s) 57, 1174
Van91I CCANNNNNTGG 1 cut(s) 1055
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 2 cut(s) 921, 1237
XceI RCATGY 4 cut(s) 199, 779, 941, 961
XspI CTAG 1 cut(s) 680
ZrmI AGTACT 1 cut(s) 1348
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.