Rorug03G0156500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001246
Physical Location & Seq
Reverse (-)
12886595 .. 12890477
3883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0156500.1

Sequence Viewer

Length: 1674 bp
ATGGCGGAAGGCGCTTCATTTTTGTTCGGCGGCATTCACTTCAACCGGAGAAAGTTCGCCACTGACTTTTCCCGATTCGAGGCCAAAAAGAAAGAGATAAACGCTTCGAACCCGGTCGAAGTTGCACAACCTGAACCAGAAGAAGAGGAGGAAGGGCCAGTAGTAGCAGTTTCTGTGAAGAAGAGGAAGAGGAAGACGGAGGCTTCAGAGTCTGGGGATGGAATCAGTCTTTTCAAGACGTCAAAATCAGCAAAGGCAGCTAAGAAAGATAAAGAGAAGCCTGCGAATGAACGCGCCAAGCAGAAGAAGAAATTAAATAGACAACTTGAGCTGGATTCGATACTTCGAAAGAAGCATAATGTGCATGTGTCGGGGAATAATATCCCATCCCCTCTTGAGAATTTTTCAGAGTTAAGCTCAAGATTTGGATGTGAAGAGTATTTACTTCGTAATTTGGCCGAGCTTGGATTTGAAGAGCCAACACCAATCCAAAGGCAGGCTATTCCAGTCCTCTTATCTGGCCGGGAATGCTTTGCTTGTGCTCCAACTGGGTCTGGGAAAACCCTTGCTTTTGTGGTTCCCATGCTTATGAAACTTAAGCACGCATCTAAGGATGGCATTCGAGCTGTGATTCTTTGTCCTACACGTGAATTAGCTGCTCAGACAACCAGAGAGTGCAAAAAATTGGCAAAAGGAAATAAATTTTACATTAAGTTGATGACTAAAGAGCTTGTTAGAAGTGCTGATTTTTCAAAAGACCGTTGTGATATACTCATATCTACTCCACAACGGTTGCGGATGGCTATTCGAAAAAAGAAGCTTAATTTAAGCAGGGTGGAATATCTCGTCTTGGATGAGTCTGATAAGCTCTTTGAGCTTGGCCTGTTAAAGCAGATTGATTCTGTGGTGAAAGCATGCTCAAACCCTTCAATAGTTCGCTCCTTGTTTAGTGCTACTTTACCTGATTTTGTTGAGGAGCTTGCACGTACTATAATGCATGATGCTGTTCGAGTAATCATTGGCAGGAAAAATACTGCTTCTGAGACTATTAAGCAAAAATTGGTATATGCTGGAAGTGAACAGGGGAAATTTCTAGCACTTCGACAGAGCTTTACAGAGAGTCTAAATCCACCAGTGTTGATCTTTGTACAAAGCAAAGAGCGAGCAAAGGAACTCTATGGAGAGCTGTTATGTGAGAACATAAGAGTTGGTGCAATCCATTCTGATATGTCGCAAGCAGAGCGAGAAAATGCTGTTGACGACTTCAGAGCTGGAAAAACGTGGGTTTTGATTGCCACTGATGTTATTGCTCGGGGTATGGATTTCAAAGGCATCAACTGTGTTATTAATTATGATTTTCCAGATTCTTCGGCTGCCTACATTCACCGGATTGGTCGCTGTGGAAGAGCGGGAAGGAGTGGAGAAGCTATTACTTTCTACACAGATGAAGATATTCCATTTTTACGGAATATAGCTAATGTTATGGCATCATCAGGTTGTGAGGTTCCATCTTGGATCATGGTCTTGCCCAAAAAGAAGTGGAGAAAGCACAGACCTGGAAGAGGAGGATCGGTATTAGCCGGACCGAAAGATGAAGTAAAAAAGAAGTGGACAAAGAAGGCAAAAAAAACCAAGACAAACATAGATGATGAACCAAAAGATGAGCAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

557

Amino Acids

62.44

Weight (kDa)

9.51

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 161 - 326 1.1e-39 DEAD/DEAH box helicase
ResIII PF04851 164 - 311 1e-10 Type III restriction enzyme, res subunit
Helicase_C PF00271 363 - 471 2.8e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 242
AccBSI CCGCTC 1 cut(s) 1409
AccII CGCG 1 cut(s) 294
AciI CCGC 4 cut(s) 5, 30, 796, 1409
AclWI GGATC 2 cut(s) 1523, 1576
AcoI YGGCCR 2 cut(s) 456, 520
AcsI RAATTY 3 cut(s) 400, 701, 1088
AcuI CTGAAG 2 cut(s) 189, 1249
AcvI CACGTG 1 cut(s) 647
AcyI GRCGYC 1 cut(s) 239
AfaI GTAC 2 cut(s) 988, 1149
AfiI CCNNNNNNNGG 3 cut(s) 79, 496, 1562
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 644
AgsI TTSAA 6 cut(s) 43, 235, 473, 753, 930, 1327
AjnI CCWGG 1 cut(s) 1555
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 1 cut(s) 1037
AlwI GGATC 2 cut(s) 1523, 1576
AlwNI CAGNNNCTG 2 cut(s) 173, 212
Ama87I CYCGRG 1 cut(s) 1311
AoxI GGCC 5 cut(s) 81, 155, 456, 520, 880
ApeKI GCWGC 3 cut(s) 257, 656, 1373
ApoI RAATTY 3 cut(s) 400, 701, 1088
AseI ATTAAT 1 cut(s) 1347
Asp700I GAANNNNTTC 1 cut(s) 1452
AspLEI GCGC 2 cut(s) 14, 296
AspS9I GGNCC 2 cut(s) 155, 1583
AsuC2I CCSGG 2 cut(s) 113, 524
AsuHPI GGTGA 2 cut(s) 919, 1376
AsuII TTCGAA 3 cut(s) 107, 346, 808
AvaI CYCGRG 1 cut(s) 1311
AvaII GGWCC 1 cut(s) 1583
BarI GAAGNNNNNNTAC 2 cut(s) 426, 458
BbrPI CACGTG 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 3 cut(s) 269, 643, 1360
BccI CCATC 5 cut(s) 212, 394, 608, 793, 1516
BciT130I CCWGG 1 cut(s) 1557
BcnI CCSGG 2 cut(s) 113, 524
BcoDI GTCTC 1 cut(s) 1037
BfaI CTAG 1 cut(s) 1094
BfoI RGCGCY 1 cut(s) 15
BfrI CTTAAG 1 cut(s) 596
BisI GCNGC 4 cut(s) 31, 258, 657, 1374
BlsI GCNGC 4 cut(s) 32, 259, 658, 1375
Bme1390I CCNGG 3 cut(s) 113, 524, 1557
Bme18I GGWCC 1 cut(s) 1583
BmeT110I CYCGRG 1 cut(s) 1311
BmgT120I GGNCC 2 cut(s) 155, 1583
BmiI GGNNCC 2 cut(s) 579, 1506
BmrFI CCNGG 3 cut(s) 113, 524, 1557
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 4 cut(s) 614, 991, 1341, 1496
BmuI ACTGGG 1 cut(s) 558
BpiI GAAGAC 1 cut(s) 200
BplI GAGNNNNNCTC 2 cut(s) 401, 433
Bpu14I TTCGAA 3 cut(s) 107, 346, 808
BpuEI CTTGAG 3 cut(s) 347, 403, 416
BpuMI CCSGG 2 cut(s) 113, 524
BsaAI YACGTR 2 cut(s) 647, 986
BsaHI GRCGYC 1 cut(s) 239
BsaWI WCCGGW 2 cut(s) 45, 1386
BsaXI ACNNNNNCTCC 2 cut(s) 1557, 1587
Bsc4I CCNNNNNNNGG 3 cut(s) 79, 496, 1562
Bse1I ACTGG 4 cut(s) 158, 506, 553, 1133
BseBI CCWGG 1 cut(s) 1557
BseGI GGATG 6 cut(s) 223, 386, 434, 619, 804, 859
BseLI CCNNNNNNNGG 3 cut(s) 79, 496, 1562
BseMII CTCAG 2 cut(s) 674, 1032
BseNI ACTGG 4 cut(s) 158, 506, 553, 1133
BseRI GAGGAG 3 cut(s) 161, 989, 1578
BseXI GCAGC 3 cut(s) 269, 643, 1360
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 5 cut(s) 83, 157, 458, 522, 882
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 1311
BsiSI CCGG 5 cut(s) 46, 113, 523, 1387, 1581
BslI CCNNNNNNNGG 3 cut(s) 79, 496, 1562
BsmAI GTCTC 1 cut(s) 1037
BsmI GAATGC 3 cut(s) 33, 533, 618
BsnI GGCC 5 cut(s) 83, 157, 458, 522, 882
BsoBI CYCGRG 1 cut(s) 1311
Bsp119I TTCGAA 3 cut(s) 107, 346, 808
Bsp1286I GDGCHC 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 1147
Bsp143I GATC 3 cut(s) 1140, 1515, 1568
BspACI CCGC 4 cut(s) 5, 30, 796, 1409
BspANI GGCC 5 cut(s) 83, 157, 458, 522, 882
BspCNI CTCAG 2 cut(s) 673, 1033
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 2 cut(s) 579, 1506
BspPI GGATC 2 cut(s) 1523, 1576
BspQI GCTCTTC 2 cut(s) 468, 1399
BspT104I TTCGAA 3 cut(s) 107, 346, 808
BspTI CTTAAG 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 1409
BsrGI TGTACA 1 cut(s) 1147
BsrI ACTGG 4 cut(s) 158, 506, 553, 1133
BssMI GATC 3 cut(s) 1140, 1515, 1568
BssNI GRCGYC 1 cut(s) 239
Bst2UI CCWGG 1 cut(s) 1557
Bst4CI ACNGT 3 cut(s) 761, 792, 1340
Bst6I CTCTTC 7 cut(s) 138, 176, 182, 429, 468, 1399, 1555
BstACI GRCGYC 1 cut(s) 239
BstAFI CTTAAG 1 cut(s) 596
BstAPI GCANNNNNTGC 1 cut(s) 361
BstAUI TGTACA 1 cut(s) 1147
BstBAI YACGTR 2 cut(s) 647, 986
BstBI TTCGAA 3 cut(s) 107, 346, 808
BstC8I GCNNGC 7 cut(s) 282, 498, 603, 916, 981, 1164, 1236
BstDEI CTNAG 4 cut(s) 261, 609, 660, 1041
BstENI CCTNNNNNAGG 1 cut(s) 1560
BstF5I GGATG 6 cut(s) 223, 386, 434, 619, 804, 859
BstFNI CGCG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 296
BstKTI GATC 3 cut(s) 1143, 1518, 1571
BstMAI GTCTC 1 cut(s) 1037
BstMBI GATC 3 cut(s) 1140, 1515, 1568
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 6 cut(s) 11, 257, 361, 528, 874, 1240
BstNI CCWGG 1 cut(s) 1557
BstNSI RCATGY 2 cut(s) 368, 918
BstSCI CCNGG 3 cut(s) 111, 522, 1555
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 269, 643, 1360
BstV2I GAAGAC 1 cut(s) 200
BsuRI GGCC 5 cut(s) 83, 157, 458, 522, 882
BtsCI GGATG 6 cut(s) 223, 386, 434, 619, 804, 859
BtsIMutI CAGTG 3 cut(s) 60, 1140, 1296
Cac8I GCNNGC 7 cut(s) 282, 498, 603, 916, 981, 1164, 1236
CaiI CAGNNNCTG 2 cut(s) 173, 212
CfoI GCGC 2 cut(s) 14, 296
Cfr13I GGNCC 2 cut(s) 155, 1583
CpoI CGGWCCG 1 cut(s) 1583
Csp6I GTAC 2 cut(s) 987, 1148
CspCI CAANNNNNGTGG 4 cut(s) 774, 809, 1119, 1154
CspI CGGWCCG 1 cut(s) 1583
CviAII CATG 5 cut(s) 365, 583, 915, 998, 1519
CviQI GTAC 2 cut(s) 987, 1148
DdeI CTNAG 4 cut(s) 261, 609, 660, 1041
DpnI GATC 3 cut(s) 1142, 1517, 1570
DpnII GATC 3 cut(s) 1140, 1515, 1568
EaeI YGGCCR 2 cut(s) 456, 520
Eam1104I CTCTTC 7 cut(s) 138, 176, 182, 429, 468, 1399, 1555
EarI CTCTTC 7 cut(s) 138, 176, 182, 429, 468, 1399, 1555
EciI GGCGGA 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 1583
Eco57I CTGAAG 2 cut(s) 189, 1249
Eco72I CACGTG 1 cut(s) 647
Eco88I CYCGRG 1 cut(s) 1311
EcoNI CCTNNNNNAGG 1 cut(s) 1560
EcoRII CCWGG 1 cut(s) 1555
EcoT22I ATGCAT 1 cut(s) 999
FaeI CATG 5 cut(s) 368, 586, 918, 1001, 1522
FatI CATG 5 cut(s) 364, 582, 914, 997, 1518
FauI CCCGC 1 cut(s) 1402
Fnu4HI GCNGC 4 cut(s) 31, 258, 657, 1374
FokI GGATG 6 cut(s) 230, 373, 441, 626, 811, 866
Fsp4HI GCNGC 4 cut(s) 31, 258, 657, 1374
FspBI CTAG 1 cut(s) 1094
GlaI GCGC 2 cut(s) 13, 295
GluI GCNGC 4 cut(s) 31, 258, 657, 1374
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 5 cut(s) 83, 157, 458, 522, 882
HapII CCGG 5 cut(s) 46, 113, 523, 1387, 1581
HhaI GCGC 2 cut(s) 14, 296
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 5 cut(s) 368, 586, 918, 1001, 1522
Hin6I GCGC 2 cut(s) 12, 294
HinP1I GCGC 2 cut(s) 12, 294
HincII GTYRAC 1 cut(s) 1258
HindII GTYRAC 1 cut(s) 1258
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 9 cut(s) 75, 209, 222, 335, 631, 857, 899, 1120, 1364
HpaII CCGG 5 cut(s) 46, 113, 523, 1387, 1581
HphI GGTGA 2 cut(s) 919, 1376
Hpy166II GTNNAC 3 cut(s) 1079, 1258, 1611
Hpy188I TCNGA 7 cut(s) 208, 409, 663, 862, 1042, 1225, 1268
Hpy188III TCNNGA 5 cut(s) 72, 235, 395, 420, 1361
Hpy8I GTNNAC 3 cut(s) 1079, 1258, 1611
HpyAV CCTTC 4 cut(s) 146, 936, 1407, 1612
HpyCH4III ACNGT 3 cut(s) 761, 792, 1340
HpyCH4IV ACGT 4 cut(s) 239, 646, 985, 1280
HpyCH4V TGCA 6 cut(s) 125, 364, 678, 983, 997, 1214
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 257, 361, 528, 874, 1240
HpyF3I CTNAG 4 cut(s) 261, 609, 660, 1041
HpySE526I ACGT 4 cut(s) 239, 646, 985, 1280
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 5 cut(s) 368, 586, 918, 1001, 1522
HspAI GCGC 2 cut(s) 12, 294
Kzo9I GATC 3 cut(s) 1140, 1515, 1568
LguI GCTCTTC 2 cut(s) 468, 1399
LmnI GCTCC 3 cut(s) 547, 944, 976
Lsp1109I GCAGC 3 cut(s) 269, 643, 1360
LweI GCATC 4 cut(s) 614, 991, 1341, 1496
MaeI CTAG 1 cut(s) 1094
MaeII ACGT 4 cut(s) 239, 646, 985, 1280
MalI GATC 3 cut(s) 1142, 1517, 1570
MbiI CCGCTC 1 cut(s) 1409
MboI GATC 3 cut(s) 1140, 1515, 1568
MhlI GDGCHC 1 cut(s) 544
MlyI GAGTC 3 cut(s) 218, 866, 1129
MmeI TCCRAC 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 999
MroXI GAANNNNTTC 1 cut(s) 1452
MseI TTAA 9 cut(s) 314, 413, 597, 711, 822, 827, 887, 1050, 1347
MslI CAYNNNNRTG 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 5 cut(s) 46, 113, 523, 1387, 1581
MspR9I CCNGG 3 cut(s) 113, 524, 1557
Mva1269I GAATGC 3 cut(s) 33, 533, 618
MvaI CCWGG 1 cut(s) 1557
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 6 cut(s) 11, 257, 361, 528, 874, 1240
NciI CCSGG 2 cut(s) 113, 524
NdeII GATC 3 cut(s) 1140, 1515, 1568
NlaIII CATG 5 cut(s) 368, 586, 918, 1001, 1522
NlaIV GGNNCC 2 cut(s) 579, 1506
NmeAIII GCCGAG 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 999
NspI RCATGY 2 cut(s) 368, 918
NspV TTCGAA 3 cut(s) 107, 346, 808
PaeI GCATGC 1 cut(s) 918
PciSI GCTCTTC 2 cut(s) 468, 1399
PctI GAATGC 3 cut(s) 33, 533, 618
PdmI GAANNNNTTC 1 cut(s) 1452
PfeI GAWTC 6 cut(s) 75, 222, 335, 631, 899, 1364
PkrI GCNGC 4 cut(s) 32, 259, 658, 1375
PleI GAGTC 3 cut(s) 217, 865, 1128
PmaCI CACGTG 1 cut(s) 647
PmlI CACGTG 1 cut(s) 647
PpsI GAGTC 3 cut(s) 217, 865, 1128
Ppu21I YACGTR 2 cut(s) 647, 986
PshBI ATTAAT 1 cut(s) 1347
Psp6I CCWGG 1 cut(s) 1555
PspCI CACGTG 1 cut(s) 647
PspGI CCWGG 1 cut(s) 1555
PspN4I GGNNCC 2 cut(s) 579, 1506
PspPI GGNCC 2 cut(s) 155, 1583
PstNI CAGNNNCTG 2 cut(s) 173, 212
RsaI GTAC 2 cut(s) 988, 1149
RsaNI GTAC 2 cut(s) 987, 1148
RseI CAYNNNNRTG 1 cut(s) 587
Rsr2I CGGWCCG 1 cut(s) 1583
RsrII CGGWCCG 1 cut(s) 1583
SapI GCTCTTC 2 cut(s) 468, 1399
SaqAI TTAA 9 cut(s) 314, 413, 597, 711, 822, 827, 887, 1050, 1347
SatI GCNGC 4 cut(s) 31, 258, 657, 1374
Sau3AI GATC 3 cut(s) 1140, 1515, 1568
Sau96I GGNCC 2 cut(s) 155, 1583
SchI GAGTC 3 cut(s) 218, 866, 1129
ScrFI CCNGG 3 cut(s) 113, 524, 1557
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 4 cut(s) 614, 991, 1341, 1496
SfuI TTCGAA 3 cut(s) 107, 346, 808
SinI GGWCC 1 cut(s) 1583
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 4 cut(s) 326, 395, 418, 596
SmoI CTYRAG 4 cut(s) 326, 395, 418, 596
SphI GCATGC 1 cut(s) 918
SsiI CCGC 4 cut(s) 5, 30, 796, 1409
SspMI CTAG 1 cut(s) 1094
StyD4I CCNGG 3 cut(s) 111, 522, 1555
TaaI ACNGT 3 cut(s) 761, 792, 1340
TaiI ACGT 4 cut(s) 242, 649, 988, 1283
TaqI TCGA 9 cut(s) 78, 107, 117, 338, 346, 622, 808, 1009, 1102
TaqII GACCGA 1 cut(s) 1600
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 6 cut(s) 75, 222, 335, 631, 899, 1364
Tru1I TTAA 9 cut(s) 314, 413, 597, 711, 822, 827, 887, 1050, 1347
Tru9I TTAA 9 cut(s) 314, 413, 597, 711, 822, 827, 887, 1050, 1347
TscAI CASTG 3 cut(s) 67, 1140, 1303
TseI GCWGC 3 cut(s) 257, 656, 1373
TspDTI ATGAA 6 cut(s) 6, 303, 605, 1461, 1608, 1665
TspGWI ACGGA 2 cut(s) 212, 1480
TspRI CASTG 3 cut(s) 67, 1140, 1303
Vha464I CTTAAG 1 cut(s) 596
VpaK11BI GGWCC 1 cut(s) 1583
VspI ATTAAT 1 cut(s) 1347
XagI CCTNNNNNAGG 1 cut(s) 1560
XapI RAATTY 3 cut(s) 400, 701, 1088
XceI RCATGY 2 cut(s) 368, 918
XmnI GAANNNNTTC 1 cut(s) 1452
XspI CTAG 1 cut(s) 1094
ZraI GACGTC 1 cut(s) 240
Zsp2I ATGCAT 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.