RLG00000023756

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001246
Physical Location & Seq
Reverse (-)
27856157 .. 27860388
4232 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023756

Sequence Viewer

Length: 1797 bp
ATGGAGAAAAAGAGAAAGATCACTCAATACAGAGAGAGGCTGGACAAGACACTGACATCGCCTAATCTAACAAATAAGGAGGCTCTAAAAGTCCTTGTCAAGGATCAGCTTTTATGTTCTTCAGAAACTGAAATTGAAGGTTGTAATGCAAATGTGATAGAACAAAGGACGGCTGAGGTGTCAAATTTTCTTGACATGCTGAGGAGTGCTTCAGATGGTGATGACAAGGGAACTTGTGATACCCTATCACATCCTGAATGGAAATTAAAACAGGACAATGAGGAGTTCCGGGTTATGTATCGTGAGGGAATCCAAGGCTCTCCATTTCATACATTACTTGTTGAAGGTTACATAGATGGGCCTGTAGATGTCTGTATGTGCATCTCATGTGAGGCAGACCTTTACAAGAAATGGTGGCCTCAGTCTACCATTCCAACTTTTAAAATTCTCTCTGGGAAGTGTTTACAGAAGGTCGGGATTGGCGAACAGATTTCTGTAGTGAGGATGAAGATTCCGTGGCCACTTTCACCTAGGGAAGCTGTTGTGCACTATTTTGTGTTTGAGTACTATGAAGATGACCTGGTTGTTGTTCTCTTGAACACAATCTCTGATTTTGGAGGCATTGATGGTGGCCTTAAAAATGAGGCAATTGATGGAGCAAAGGATGTAGTTAGGATAGATGTAGTGGGTGGCTTTGCTTTGCAGAAGGTTACCGATGATAGAAGTTACTTTCGGACAATAGCAAATATGGATATAAAGATGGACTTGATCCCACCATCCCTTATAAATTTTATCTCAAGGCAGCTCATTGGCAATGGTTTCAGACTGTATCAAAAGGTAGTGTCTTCTAAGCTTAATCGTGATGAAGATTACAGCAAGGCTTTGAGAGGCCCACTATACTCTCGCATACGTGAAGCTCTTTCTTCACTTCATGAATCAAGCAGGCTTCTGGAGGGAAAAAAGCTAAATGGTGATGCATCCAATCTCTCTGAAGAACATCTAGTTAAAAATAAAATGAATGATTTGAAGTTGGTGGGTATGGATGAGAAAGTTCATGATGAACACCTTGCAAGTGAAGCTACCCCAGATGATGCACATGTTACTGGAGGAAACACTCTTTGTGAGATTGAGGAGGTAGAGAGTGAAGGAAGCAAGACTGAGGAGATTAGAAGCGAAAAAAGCAAGATTGAGGTGGTAGAGAGCAAAGAAAGCAGGCAGCTTGAGGATCAAACATCCAACAGAATTCCAGAAAGGGGACATGTAAATGGAAAGAGAAACGTGTTTATCAGTTCTGAGGTGGAAGAAGCTATAGGAACAATAGAAAGGGTCATTTTGAGGGTTAGGCAATATAGATTCAGCGCTGAAATGCCAGCCCCTGGTTTCACCAATGAAGTCTTACGAAAGGAAAATGATGGACGTAACCCAGAGTCCTTAGAAGATGAAGTCCGCTTAAGTGAACATTTTTCTGCAGTACCTAAAGAAGAGGACACTGAAGGGGTGTTGACTACACCTTTAAGGAATAGCACTGCTGTTCAAAACATAAGTTATGCAGGCTCAAATTACTTGTCAAAGGATGCTAACCATAACAGAATAGTACCCACCACCCCATTGTGTTCCTCCAAAGATGGGATTGCTGAGTTACCAGTAGTGGATAACATCATGCATAGTACTAACCAAATTAGCTGCAAGACAAATGACACTCATCAAAGTAGTCCCAGTAGAAGGAAGAAGTCAGGGCGGCAAAGAAGGCTTGGGTTCTGTTGCTTCAACGTCAATTCGGGAGAGATAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

599

Amino Acids

67.3

Weight (kDa)

5.43

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 785
AccB7I CCANNNNNTGG 1 cut(s) 1376
AccI GTMKAC 1 cut(s) 425
AciI CCGC 2 cut(s) 1447, 1738
AclWI GGATC 3 cut(s) 111, 763, 1233
AcoI YGGCCR 1 cut(s) 518
AcsI RAATTY 4 cut(s) 184, 444, 787, 1242
AcuI CTGAAG 4 cut(s) 105, 195, 1011, 1512
AfaI GTAC 4 cut(s) 566, 1473, 1596, 1669
AfeI AGCGCT 1 cut(s) 1360
AfiI CCNNNNNNNGG 5 cut(s) 100, 1253, 1376, 1626, 1722
AflII CTTAAG 1 cut(s) 1450
AflIII ACRYGT 3 cut(s) 1096, 1258, 1278
AgsI TTSAA 6 cut(s) 137, 344, 598, 1027, 1535, 1768
AjnI CCWGG 2 cut(s) 579, 1375
Alw21I GWGCWC 1 cut(s) 549
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 3 cut(s) 111, 763, 1233
AlwNI CAGNNNCTG 2 cut(s) 128, 1376
Aor51HI AGCGCT 1 cut(s) 1360
AoxI GGCC 5 cut(s) 359, 416, 518, 631, 889
ApaLI GTGCAC 1 cut(s) 545
ApeKI GCWGC 3 cut(s) 802, 1216, 1683
ApoI RAATTY 4 cut(s) 184, 444, 787, 1242
ArsI GACNNNNNNTTYG 2 cut(s) 1550, 1582
AspA2I CCTAGG 1 cut(s) 530
AspLEI GCGC 1 cut(s) 1361
AspS9I GGNCC 2 cut(s) 359, 890
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 4 cut(s) 230, 519, 983, 1375
AvrII CCTAGG 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 837
Bbv12I GWGCWC 1 cut(s) 549
BbvCI CCTCAGC 2 cut(s) 174, 200
BbvI GCAGC 3 cut(s) 814, 1228, 1670
BccI CCATC 8 cut(s) 209, 350, 620, 647, 754, 784, 1406, 1619
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 2 cut(s) 581, 1377
BcnI CCSGG 1 cut(s) 290
BfaI CTAG 2 cut(s) 531, 1001
BfmI CTRYAG 4 cut(s) 363, 495, 1308, 1467
BfoI RGCGCY 1 cut(s) 1362
BfrI CTTAAG 1 cut(s) 1450
BisI GCNGC 4 cut(s) 803, 1217, 1684, 1739
BlnI CCTAGG 1 cut(s) 530
BlsI GCNGC 4 cut(s) 804, 1218, 1685, 1740
BmcAI AGTACT 2 cut(s) 566, 1669
Bme1390I CCNGG 3 cut(s) 290, 581, 1377
BmgT120I GGNCC 2 cut(s) 359, 890
BmrFI CCNGG 3 cut(s) 290, 581, 1377
BmrI ACTGGG 1 cut(s) 1710
BmsI GCATC 5 cut(s) 390, 964, 986, 1081, 1564
BmuI ACTGGG 1 cut(s) 1710
BpiI GAAGAC 1 cut(s) 837
BpmI CTGGAG 2 cut(s) 971, 1125
Bpu10I CCTNAGC 2 cut(s) 174, 200
BpuEI CTTGAG 2 cut(s) 781, 1241
BpuMI CCSGG 1 cut(s) 290
BsaAI YACGTR 1 cut(s) 911
BsaJI CCNNGG 4 cut(s) 313, 515, 530, 1375
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 1253, 1376, 1626, 1722
Bse1I ACTGG 3 cut(s) 1108, 1643, 1716
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 2 cut(s) 581, 1377
BseDI CCNNGG 4 cut(s) 313, 515, 530, 1375
BseGI GGATG 8 cut(s) 250, 510, 670, 776, 977, 1048, 1232, 1579
BseLI CCNNNNNNNGG 5 cut(s) 100, 1253, 1376, 1626, 1722
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 6 cut(s) 165, 191, 434, 1149, 1284, 1626
BseNI ACTGG 3 cut(s) 1108, 1643, 1716
BseRI GAGGAG 4 cut(s) 217, 296, 1145, 1175
BseSI GKGCMC 1 cut(s) 549
BseXI GCAGC 3 cut(s) 814, 1228, 1670
BshFI GGCC 5 cut(s) 361, 418, 520, 633, 891
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 1 cut(s) 289
BslFI GGGAC 2 cut(s) 1269, 1698
BslI CCNNNNNNNGG 5 cut(s) 100, 1253, 1376, 1626, 1722
BsmFI GGGAC 2 cut(s) 1269, 1698
BsnI GGCC 5 cut(s) 361, 418, 520, 633, 891
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 4 cut(s) 18, 103, 768, 1225
BspACI CCGC 2 cut(s) 1447, 1738
BspANI GGCC 5 cut(s) 361, 418, 520, 633, 891
BspCNI CTCAG 6 cut(s) 166, 192, 433, 1150, 1285, 1627
BspHI TCATGA 2 cut(s) 931, 1054
BspMAI CTGCAG 1 cut(s) 1471
BspPI GGATC 3 cut(s) 111, 763, 1233
BspTI CTTAAG 1 cut(s) 1450
BsrDI GCAATG 1 cut(s) 820
BsrI ACTGG 3 cut(s) 1108, 1643, 1716
BssECI CCNNGG 4 cut(s) 313, 515, 530, 1375
BssMI GATC 4 cut(s) 18, 103, 768, 1225
BssT1I CCWWGG 2 cut(s) 313, 530
Bst2UI CCWGG 2 cut(s) 581, 1377
Bst4CI ACNGT 1 cut(s) 828
Bst6I CTCTTC 1 cut(s) 1476
BstAFI CTTAAG 1 cut(s) 1450
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 4 cut(s) 944, 1214, 1371, 1552
BstDEI CTNAG 8 cut(s) 174, 200, 420, 849, 1158, 1293, 1432, 1635
BstDSI CCRYGG 1 cut(s) 515
BstEII GGTNACC 1 cut(s) 709
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 8 cut(s) 250, 510, 670, 776, 977, 1048, 1232, 1579
BstH2I RGCGCY 1 cut(s) 1362
BstHHI GCGC 1 cut(s) 1361
BstKTI GATC 4 cut(s) 21, 106, 771, 1228
BstMBI GATC 4 cut(s) 18, 103, 768, 1225
BstMWI GCNNNNNNNGC 4 cut(s) 1076, 1179, 1209, 1747
BstNI CCWGG 2 cut(s) 581, 1377
BstNSI RCATGY 3 cut(s) 199, 1100, 1262
BstPI GGTNACC 1 cut(s) 709
BstSCI CCNGG 3 cut(s) 288, 579, 1375
BstSFI CTRYAG 4 cut(s) 363, 495, 1308, 1467
BstSLI GKGCMC 1 cut(s) 549
BstV1I GCAGC 3 cut(s) 814, 1228, 1670
BstV2I GAAGAC 1 cut(s) 837
BsuRI GGCC 5 cut(s) 361, 418, 520, 633, 891
BtgI CCRYGG 1 cut(s) 515
BtgZI GCGATG 1 cut(s) 42
BtsCI GGATG 8 cut(s) 250, 510, 670, 776, 977, 1048, 1232, 1579
BtsI GCAGTG 1 cut(s) 1524
BtsIMutI CAGTG 3 cut(s) 50, 1488, 1524
Cac8I GCNNGC 4 cut(s) 944, 1214, 1371, 1552
CaiI CAGNNNCTG 2 cut(s) 128, 1376
CciI TCATGA 2 cut(s) 931, 1054
CfoI GCGC 1 cut(s) 1361
Cfr13I GGNCC 2 cut(s) 359, 890
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 4 cut(s) 565, 1472, 1595, 1668
CviAII CATG 7 cut(s) 196, 387, 932, 1055, 1097, 1259, 1660
CviQI GTAC 4 cut(s) 565, 1472, 1595, 1668
DdeI CTNAG 8 cut(s) 174, 200, 420, 849, 1158, 1293, 1432, 1635
DpnI GATC 4 cut(s) 20, 105, 770, 1227
DpnII GATC 4 cut(s) 18, 103, 768, 1225
DraI TTTAAA 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 518
Eam1104I CTCTTC 1 cut(s) 1476
EarI CTCTTC 1 cut(s) 1476
Eco130I CCWWGG 2 cut(s) 313, 530
Eco47III AGCGCT 1 cut(s) 1360
Eco57I CTGAAG 4 cut(s) 105, 195, 1011, 1512
Eco91I GGTNACC 1 cut(s) 709
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 709
EcoRI GAATTC 1 cut(s) 1242
EcoRII CCWGG 2 cut(s) 579, 1375
EcoT14I CCWWGG 2 cut(s) 313, 530
EcoT22I ATGCAT 2 cut(s) 979, 1665
ErhI CCWWGG 2 cut(s) 313, 530
FaeI CATG 7 cut(s) 199, 390, 935, 1058, 1100, 1262, 1663
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 2 cut(s) 1269, 1698
FatI CATG 7 cut(s) 195, 386, 931, 1054, 1096, 1258, 1659
FblI GTMKAC 1 cut(s) 425
Fnu4HI GCNGC 4 cut(s) 803, 1217, 1684, 1739
FokI GGATG 8 cut(s) 237, 517, 677, 763, 964, 1055, 1219, 1586
Fsp4HI GCNGC 4 cut(s) 803, 1217, 1684, 1739
FspBI CTAG 2 cut(s) 531, 1001
GlaI GCGC 1 cut(s) 1360
GluI GCNGC 4 cut(s) 803, 1217, 1684, 1739
GsuI CTGGAG 2 cut(s) 971, 1125
HaeII RGCGCY 1 cut(s) 1362
HaeIII GGCC 5 cut(s) 361, 418, 520, 633, 891
HapII CCGG 1 cut(s) 289
HhaI GCGC 1 cut(s) 1361
Hin1II CATG 7 cut(s) 199, 390, 935, 1058, 1100, 1262, 1663
Hin6I GCGC 1 cut(s) 1359
HinP1I GCGC 1 cut(s) 1359
HincII GTYRAC 1 cut(s) 1503
HindII GTYRAC 1 cut(s) 1503
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 6 cut(s) 309, 511, 935, 1353, 1427, 1790
HpaII CCGG 1 cut(s) 289
HphI GGTGA 4 cut(s) 230, 519, 983, 1375
Hpy166II GTNNAC 5 cut(s) 426, 464, 547, 1457, 1503
Hpy188I TCNGA 7 cut(s) 124, 214, 610, 735, 824, 991, 1294
Hpy8I GTNNAC 5 cut(s) 426, 464, 547, 1457, 1503
HpyAV CCTTC 8 cut(s) 131, 338, 463, 700, 1139, 1487, 1716, 1740
HpyCH4III ACNGT 1 cut(s) 828
HpyCH4IV ACGT 4 cut(s) 910, 1278, 1417, 1770
HpyF10VI GCNNNNNNNGC 4 cut(s) 1076, 1179, 1209, 1747
HpyF3I CTNAG 8 cut(s) 174, 200, 420, 849, 1158, 1293, 1432, 1635
HpySE526I ACGT 4 cut(s) 910, 1278, 1417, 1770
Hsp92II CATG 7 cut(s) 199, 390, 935, 1058, 1100, 1262, 1663
HspAI GCGC 1 cut(s) 1359
Kzo9I GATC 4 cut(s) 18, 103, 768, 1225
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 3 cut(s) 814, 1228, 1670
LweI GCATC 5 cut(s) 390, 964, 986, 1081, 1564
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 2 cut(s) 531, 1001
MaeII ACGT 4 cut(s) 910, 1278, 1417, 1770
MaeIII GTNAC 6 cut(s) 347, 709, 725, 1099, 1418, 1638
MalI GATC 4 cut(s) 20, 105, 770, 1227
MboI GATC 4 cut(s) 18, 103, 768, 1225
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 1 cut(s) 520
MluNI TGGCCA 1 cut(s) 520
MlyI GAGTC 1 cut(s) 1436
MmeI TCCRAC 2 cut(s) 458, 1260
Mox20I TGGCCA 1 cut(s) 520
Mph1103I ATGCAT 2 cut(s) 979, 1665
MscI TGGCCA 1 cut(s) 520
MseI TTAA 8 cut(s) 266, 441, 636, 855, 1005, 1451, 1514, 1795
MslI CAYNNNNRTG 1 cut(s) 1263
Msp20I TGGCCA 1 cut(s) 520
MspCI CTTAAG 1 cut(s) 1450
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 3 cut(s) 290, 581, 1377
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 2 cut(s) 581, 1377
MwoI GCNNNNNNNGC 4 cut(s) 1076, 1179, 1209, 1747
NciI CCSGG 1 cut(s) 290
NdeII GATC 4 cut(s) 18, 103, 768, 1225
NlaIII CATG 7 cut(s) 199, 390, 935, 1058, 1100, 1262, 1663
NsiI ATGCAT 2 cut(s) 979, 1665
NspI RCATGY 3 cut(s) 199, 1100, 1262
PagI TCATGA 2 cut(s) 931, 1054
PciI ACATGT 2 cut(s) 1096, 1258
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PfeI GAWTC 5 cut(s) 309, 511, 935, 1353, 1790
PflMI CCANNNNNTGG 1 cut(s) 1376
PkrI GCNGC 4 cut(s) 804, 1218, 1685, 1740
PleI GAGTC 1 cut(s) 1435
PpsI GAGTC 1 cut(s) 1435
Ppu21I YACGTR 1 cut(s) 911
PscI ACATGT 2 cut(s) 1096, 1258
PsiI TTATAA 1 cut(s) 785
Psp6I CCWGG 2 cut(s) 579, 1375
PspEI GGTNACC 1 cut(s) 709
PspGI CCWGG 2 cut(s) 579, 1375
PspPI GGNCC 2 cut(s) 359, 890
PsrI GAACNNNNNNTAC 2 cut(s) 223, 255
PstI CTGCAG 1 cut(s) 1471
PstNI CAGNNNCTG 2 cut(s) 128, 1376
RsaI GTAC 4 cut(s) 566, 1473, 1596, 1669
RsaNI GTAC 4 cut(s) 565, 1472, 1595, 1668
RseI CAYNNNNRTG 1 cut(s) 1263
SaqAI TTAA 8 cut(s) 266, 441, 636, 855, 1005, 1451, 1514, 1795
SatI GCNGC 4 cut(s) 803, 1217, 1684, 1739
Sau3AI GATC 4 cut(s) 18, 103, 768, 1225
Sau96I GGNCC 2 cut(s) 359, 890
ScaI AGTACT 2 cut(s) 566, 1669
SchI GAGTC 1 cut(s) 1436
ScrFI CCNGG 3 cut(s) 290, 581, 1377
SduI GDGCHC 1 cut(s) 549
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 5 cut(s) 390, 964, 986, 1081, 1564
SfcI CTRYAG 4 cut(s) 363, 495, 1308, 1467
SmiMI CAYNNNNRTG 1 cut(s) 1263
SmlI CTYRAG 3 cut(s) 796, 1220, 1450
SmoI CTYRAG 3 cut(s) 796, 1220, 1450
SsiI CCGC 2 cut(s) 1447, 1738
SspMI CTAG 2 cut(s) 531, 1001
StyD4I CCNGG 3 cut(s) 288, 579, 1375
StyI CCWWGG 2 cut(s) 313, 530
TaaI ACNGT 1 cut(s) 828
TaiI ACGT 4 cut(s) 913, 1281, 1420, 1773
TatI WGTACW 2 cut(s) 564, 1667
TauI GCSGC 1 cut(s) 1741
TfiI GAWTC 5 cut(s) 309, 511, 935, 1353, 1790
Tru1I TTAA 8 cut(s) 266, 441, 636, 855, 1005, 1451, 1514, 1795
Tru9I TTAA 8 cut(s) 266, 441, 636, 855, 1005, 1451, 1514, 1795
TscAI CASTG 3 cut(s) 57, 1495, 1531
TseI GCWGC 3 cut(s) 802, 1216, 1683
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 3 cut(s) 57, 1495, 1531
Van91I CCANNNNNTGG 1 cut(s) 1376
Vha464I CTTAAG 1 cut(s) 1450
VneI GTGCAC 1 cut(s) 545
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 4 cut(s) 184, 444, 787, 1242
XceI RCATGY 3 cut(s) 199, 1100, 1262
XmaJI CCTAGG 1 cut(s) 530
XmiI GTMKAC 1 cut(s) 425
XspI CTAG 2 cut(s) 531, 1001
ZrmI AGTACT 2 cut(s) 566, 1669
Zsp2I ATGCAT 2 cut(s) 979, 1665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.