Rroxscaffold_3G00235830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001246
Physical Location & Seq
Reverse (-)
22957343 .. 22962724
5382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00235830.1

Sequence Viewer

Length: 1701 bp
ATGCCCAAGGAAAGGGCGTCTGTCACATCAGAATTTTGGAAAGAGGGGTACTTGAAGTGGGCGGTGGACTTGTGGGCTGAAGGGAGCTGCTTGGAACTGAATCAGTTTATTGTTCATGATATTGGAATTGATGTTTACGTTCAGCTTCTATTGGCTCAGTATGCTGTGGACTTGAATGATGGTCATTTGTTTATGAACAATGAGAGTTTGGCTGGAAATGCAAAGATGGAGAAAAAGAGAAAAATCACTCAATACAGAGAGAGGCTGGACAAGACACTGACATCTCCTAATCTAACAAATAAGGAGGCTCTAAAAGTCCTTGTCAAGGATCAGCTTTTGCGTTCTTCAGAAAATGAAATTGAAGGTTGTAATGCAAATGTGATAGAAGAAAGGACTCGGCAGGTGTCAAATCTGCTTGACATGTTGAGGAGTGTTTCAGTTGGTGATGACGAGGGGTTGAAAACTTGTGATACCCCATCACGTCCTGAATGGAAATTAAAACAGGACAATGAGGAGTTCCGGGTTATGTATCGTGAGGGAATCCAAGGTTCTCCATTTCATACATTACTTGTTGAAGGTTACATTGATGGGCCTGTAGATGTCTGTTTGTGCATCTCGTGTGAGGCAGACCTTTACAAGAAATGGTGGCCTCAGTCTAGCATCCCAACTTTCAAAATTCTCTCTGGGAAGTGTTTACAGAAGGTCGGGATTGGTGAACAGATATCTGTAGTGAGGATGAAGATTCCGTGGCCACTTTCACCTAGGGAAGCTGTTGTGCACTATTTTGTGTTTGAGTACTATGAAGATGACCTGATTGTTGTTCTCTTGAACACGATCTCTGATTTTGGAGGCGTTGATGGTGGCCTTCAAAATGAGGCAACTGATGGAGTAAAGGGTGCAGTTAGGATAGATGTAGTTGGTGGCTTTGCTTTGCAGAAGGTTACCGATGATAGAAGTTACATTCGGACAATAGCAAATATGGATATAAAGATGGATTTTGTCCCACCATCCCTTATAAATTTTGTCTCAAGACAGCTCATTGGCAATGGTTTCAGACTGTATCAAAAGGTTGTAGTGTCTTCCAAGCTTAATCGTGATGAAGAGTACAGCAAGGCATTCAGAGGCCCACTATACTCTCGCATACGTGAAGCTCTTTATTCACTTCATGAATCAAGCCGGCTTCTGGAGGGCAAAAAATTAAACGGTGATGCATCCAATCTCTCTGAAGAAAATCTAGTTAAACATAAAATGAATGATTTGAAGTTGGTGGATATGAATGAGAAAGTTCATAATGAACACCTTGCAAGTGAAGCTACCCCAGATGATGCACATGTTATTGGAGGAAACACTCTTTGTGAGATTGAGGAGGTAGAGAGTGAAGAAAGCAAGACTGAGGAGATTAAAAGCGAAAAAAGCAAGATTGAGGAGTCAGAGGGCAAAGAAAACAGGCATCAAACATTCAAAAGAATTCCCGAAAGGGGACATGTAATTGGAAAGAGAAACGTGTTCATCAGTTCTGAGGTGGAAGAAGCTATAGGAACAATAGAAAAAGTCATTTTGAGGGTTAGGCAATATAGGTTCGGCGCTGAAATGCCATCCTCTGGTTTCACCAATGAAGTCTCACAAAAGGAAAATGATGGATGTAACCCAGAGGCCTTAGAAGATGAAGTCCGCTCAAGAACATTTTTCTGCAGTACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

566

Amino Acids

64.28

Weight (kDa)

5.23

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 1601
AccBSI CCGCTC 1 cut(s) 1674
AciI CCGC 2 cut(s) 62, 1672
AclWI GGATC 1 cut(s) 336
AcoI YGGCCR 1 cut(s) 749
AcsI RAATTY 4 cut(s) 32, 675, 1018, 1467
AcuI CTGAAG 3 cut(s) 99, 330, 1245
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 4 cut(s) 50, 797, 1106, 1696
AfiI CCNNNNNNNGG 5 cut(s) 12, 325, 1183, 1478, 1601
AflIII ACRYGT 4 cut(s) 420, 1330, 1483, 1503
AjiI CACGTC 1 cut(s) 482
AluBI AGCT 9 cut(s) 87, 145, 334, 770, 1036, 1087, 1151, 1313, 1532
AluI AGCT 9 cut(s) 87, 145, 334, 770, 1036, 1087, 1151, 1313, 1532
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 2 cut(s) 1030, 1624
Alw44I GTGCAC 1 cut(s) 776
AlwI GGATC 1 cut(s) 336
AoxI GGCC 6 cut(s) 590, 647, 749, 862, 1123, 1653
ApaLI GTGCAC 1 cut(s) 776
ApeKI GCWGC 1 cut(s) 87
ApoI RAATTY 4 cut(s) 32, 675, 1018, 1467
AspA2I CCTAGG 1 cut(s) 761
AspLEI GCGC 1 cut(s) 1586
AspS9I GGNCC 2 cut(s) 590, 1124
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 5 cut(s) 455, 725, 750, 1217, 1600
AvrII CCTAGG 1 cut(s) 761
BaeGI GKGCMC 1 cut(s) 780
BalI TGGCCA 1 cut(s) 751
BauI CACGAG 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 1071
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 1 cut(s) 74
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 2 cut(s) 1030, 1624
BfaI CTAG 3 cut(s) 657, 762, 1235
BfmI CTRYAG 4 cut(s) 594, 726, 1533, 1690
BfoI RGCGCY 1 cut(s) 1587
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 1 cut(s) 88
BlnI CCTAGG 1 cut(s) 761
BlsI GCNGC 1 cut(s) 89
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 1 cut(s) 521
BmgBI CACGTC 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 590, 1124
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 6 cut(s) 621, 669, 1198, 1220, 1315, 1459
BpiI GAAGAC 1 cut(s) 1071
BpmI CTGGAG 1 cut(s) 1205
BpuEI CTTGAG 2 cut(s) 1012, 1660
BpuMI CCSGG 1 cut(s) 521
BsaAI YACGTR 1 cut(s) 1145
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 6, 544, 746, 761
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 325, 1183, 1478, 1601
Bse118I RCCGGY 1 cut(s) 1176
Bse3DI GCAATG 1 cut(s) 1051
BseDI CCNNGG 4 cut(s) 6, 544, 746, 761
BseGI GGATG 6 cut(s) 660, 741, 1007, 1211, 1595, 1646
BseLI CCNNNNNNNGG 5 cut(s) 12, 325, 1183, 1478, 1601
BseMI GCAATG 1 cut(s) 1051
BseMII CTCAG 4 cut(s) 170, 665, 1383, 1509
BseRI GAGGAG 5 cut(s) 442, 527, 1379, 1409, 1439
BseSI GKGCMC 1 cut(s) 780
BseXI GCAGC 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 918
BshFI GGCC 6 cut(s) 592, 649, 751, 864, 1125, 1655
BsiHKAI GWGCWC 1 cut(s) 780
BsiSI CCGG 2 cut(s) 520, 1177
BslFI GGGAC 2 cut(s) 986, 1494
BslI CCNNNNNNNGG 5 cut(s) 12, 325, 1183, 1478, 1601
BsmAI GTCTC 2 cut(s) 1030, 1624
BsmFI GGGAC 2 cut(s) 986, 1494
BsmI GAATGC 1 cut(s) 1115
BsnI GGCC 6 cut(s) 592, 649, 751, 864, 1125, 1655
Bsp1286I GDGCHC 1 cut(s) 780
Bsp143I GATC 2 cut(s) 328, 834
BspACI CCGC 2 cut(s) 62, 1672
BspANI GGCC 6 cut(s) 592, 649, 751, 864, 1125, 1655
BspCNI CTCAG 4 cut(s) 169, 664, 1384, 1510
BspHI TCATGA 2 cut(s) 115, 1165
BspMAI CTGCAG 1 cut(s) 1694
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 1 cut(s) 336
BsrBI CCGCTC 1 cut(s) 1674
BsrDI GCAATG 1 cut(s) 1051
BsrFI RCCGGY 1 cut(s) 1176
BssAI RCCGGY 1 cut(s) 1176
BssECI CCNNGG 4 cut(s) 6, 544, 746, 761
BssMI GATC 2 cut(s) 328, 834
BssNI GRCGYC 1 cut(s) 17
BssSI CACGAG 1 cut(s) 616
BssT1I CCWWGG 3 cut(s) 6, 544, 761
Bst2BI CACGAG 1 cut(s) 616
Bst4CI ACNGT 2 cut(s) 1059, 1205
Bst6I CTCTTC 1 cut(s) 1095
BstACI GRCGYC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 1145
BstC8I GCNNGC 1 cut(s) 1178
BstDEI CTNAG 5 cut(s) 156, 651, 1392, 1518, 1657
BstDSI CCRYGG 1 cut(s) 746
BstEII GGTNACC 1 cut(s) 940
BstENI CCTNNNNNAGG 1 cut(s) 323
BstF5I GGATG 6 cut(s) 660, 741, 1007, 1211, 1595, 1646
BstH2I RGCGCY 1 cut(s) 1587
BstHHI GCGC 1 cut(s) 1586
BstKTI GATC 2 cut(s) 331, 837
BstMAI GTCTC 2 cut(s) 1030, 1624
BstMBI GATC 2 cut(s) 328, 834
BstMWI GCNNNNNNNGC 4 cut(s) 161, 218, 1310, 1413
BstNSI RCATGY 3 cut(s) 424, 1334, 1487
BstPI GGTNACC 1 cut(s) 940
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 4 cut(s) 594, 726, 1533, 1690
BstSLI GKGCMC 1 cut(s) 780
BstV1I GCAGC 1 cut(s) 74
BstV2I GAAGAC 1 cut(s) 1071
BsuRI GGCC 6 cut(s) 592, 649, 751, 864, 1125, 1655
BtgI CCRYGG 1 cut(s) 746
BtrI CACGTC 1 cut(s) 482
BtsCI GGATG 6 cut(s) 660, 741, 1007, 1211, 1595, 1646
BtsIMutI CAGTG 1 cut(s) 275
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 1178
CciI TCATGA 2 cut(s) 115, 1165
CfoI GCGC 1 cut(s) 1586
Cfr10I RCCGGY 1 cut(s) 1176
Cfr13I GGNCC 2 cut(s) 590, 1124
CseI GACGC 1 cut(s) 6
Csp6I GTAC 4 cut(s) 49, 796, 1105, 1695
CviAII CATG 5 cut(s) 116, 421, 1166, 1331, 1484
CviQI GTAC 4 cut(s) 49, 796, 1105, 1695
DdeI CTNAG 5 cut(s) 156, 651, 1392, 1518, 1657
DpnI GATC 2 cut(s) 330, 836
DpnII GATC 2 cut(s) 328, 834
EaeI YGGCCR 1 cut(s) 749
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
Eco130I CCWWGG 3 cut(s) 6, 544, 761
Eco147I AGGCCT 1 cut(s) 1655
Eco32I GATATC 1 cut(s) 723
Eco57I CTGAAG 3 cut(s) 99, 330, 1245
Eco91I GGTNACC 1 cut(s) 940
EcoNI CCTNNNNNAGG 1 cut(s) 323
EcoO65I GGTNACC 1 cut(s) 940
EcoRI GAATTC 1 cut(s) 1467
EcoRV GATATC 1 cut(s) 723
EcoT14I CCWWGG 3 cut(s) 6, 544, 761
EcoT22I ATGCAT 1 cut(s) 1213
ErhI CCWWGG 3 cut(s) 6, 544, 761
FaeI CATG 5 cut(s) 119, 424, 1169, 1334, 1487
FaqI GGGAC 2 cut(s) 986, 1494
FatI CATG 5 cut(s) 115, 420, 1165, 1330, 1483
Fnu4HI GCNGC 1 cut(s) 88
FokI GGATG 6 cut(s) 647, 748, 994, 1198, 1582, 1653
Fsp4HI GCNGC 1 cut(s) 88
FspBI CTAG 3 cut(s) 657, 762, 1235
GlaI GCGC 1 cut(s) 1585
GluI GCNGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 1205
HaeII RGCGCY 1 cut(s) 1587
HaeIII GGCC 6 cut(s) 592, 649, 751, 864, 1125, 1655
HapII CCGG 2 cut(s) 520, 1177
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 1586
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 5 cut(s) 119, 424, 1169, 1334, 1487
Hin6I GCGC 1 cut(s) 1584
HinP1I GCGC 1 cut(s) 1584
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 6 cut(s) 100, 394, 540, 742, 1169, 1427
HpaII CCGG 2 cut(s) 520, 1177
HphI GGTGA 5 cut(s) 455, 725, 750, 1217, 1600
Hpy166II GTNNAC 6 cut(s) 67, 136, 169, 695, 716, 778
Hpy188I TCNGA 9 cut(s) 31, 349, 841, 966, 1055, 1121, 1225, 1432, 1519
Hpy8I GTNNAC 6 cut(s) 67, 136, 169, 695, 716, 778
HpyAV CCTTC 6 cut(s) 74, 356, 569, 694, 875, 931
HpyCH4III ACNGT 2 cut(s) 1059, 1205
HpyCH4IV ACGT 4 cut(s) 138, 481, 1144, 1503
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 218, 1310, 1413
HpyF3I CTNAG 5 cut(s) 156, 651, 1392, 1518, 1657
HpySE526I ACGT 4 cut(s) 138, 481, 1144, 1503
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 5 cut(s) 119, 424, 1169, 1334, 1487
HspAI GCGC 1 cut(s) 1584
KroI GCCGGC 1 cut(s) 1176
KroNI GCCGGC 1 cut(s) 1178
Kzo9I GATC 2 cut(s) 328, 834
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 1 cut(s) 74
LweI GCATC 6 cut(s) 621, 669, 1198, 1220, 1315, 1459
MaeI CTAG 3 cut(s) 657, 762, 1235
MaeII ACGT 4 cut(s) 138, 481, 1144, 1503
MaeIII GTNAC 5 cut(s) 22, 578, 940, 956, 1643
MalI GATC 2 cut(s) 330, 836
MbiI CCGCTC 1 cut(s) 1674
MboI GATC 2 cut(s) 328, 834
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 1 cut(s) 751
MluCI AATT 9 cut(s) 32, 126, 357, 494, 675, 1018, 1196, 1467, 1488
MluNI TGGCCA 1 cut(s) 751
MlyI GAGTC 2 cut(s) 388, 1436
Mox20I TGGCCA 1 cut(s) 751
Mph1103I ATGCAT 1 cut(s) 1213
MroNI GCCGGC 1 cut(s) 1176
MscI TGGCCA 1 cut(s) 751
MseI TTAA 5 cut(s) 497, 1089, 1199, 1239, 1401
Msp20I TGGCCA 1 cut(s) 751
MspI CCGG 2 cut(s) 520, 1177
MspR9I CCNGG 1 cut(s) 521
Mva1269I GAATGC 1 cut(s) 1115
MwoI GCNNNNNNNGC 4 cut(s) 161, 218, 1310, 1413
NaeI GCCGGC 1 cut(s) 1178
NciI CCSGG 1 cut(s) 521
NdeII GATC 2 cut(s) 328, 834
NgoMIV GCCGGC 1 cut(s) 1176
NlaIII CATG 5 cut(s) 119, 424, 1169, 1334, 1487
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 22
NsiI ATGCAT 1 cut(s) 1213
NspI RCATGY 3 cut(s) 424, 1334, 1487
PagI TCATGA 2 cut(s) 115, 1165
PaqCI CACCTGC 1 cut(s) 391
PceI AGGCCT 1 cut(s) 1655
PciI ACATGT 3 cut(s) 420, 1330, 1483
PctI GAATGC 1 cut(s) 1115
PdiI GCCGGC 1 cut(s) 1178
PfeI GAWTC 4 cut(s) 100, 540, 742, 1169
PflMI CCANNNNNTGG 1 cut(s) 1601
PkrI GCNGC 1 cut(s) 89
PleI GAGTC 2 cut(s) 388, 1435
PpsI GAGTC 2 cut(s) 388, 1435
Ppu21I YACGTR 1 cut(s) 1145
PscI ACATGT 3 cut(s) 420, 1330, 1483
PsiI TTATAA 1 cut(s) 1016
PspEI GGTNACC 1 cut(s) 940
PspPI GGNCC 2 cut(s) 590, 1124
PstI CTGCAG 1 cut(s) 1694
RsaI GTAC 4 cut(s) 50, 797, 1106, 1696
RsaNI GTAC 4 cut(s) 49, 796, 1105, 1695
SaqAI TTAA 5 cut(s) 497, 1089, 1199, 1239, 1401
SatI GCNGC 1 cut(s) 88
Sau3AI GATC 2 cut(s) 328, 834
Sau96I GGNCC 2 cut(s) 590, 1124
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 2 cut(s) 388, 1436
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 6 cut(s) 621, 669, 1198, 1220, 1315, 1459
SfcI CTRYAG 4 cut(s) 594, 726, 1533, 1690
SmlI CTYRAG 2 cut(s) 1027, 1675
SmoI CTYRAG 2 cut(s) 1027, 1675
Sse9I AATT 9 cut(s) 32, 126, 357, 494, 675, 1018, 1196, 1467, 1488
SseBI AGGCCT 1 cut(s) 1655
SsiI CCGC 2 cut(s) 62, 1672
SspMI CTAG 3 cut(s) 657, 762, 1235
StuI AGGCCT 1 cut(s) 1655
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 3 cut(s) 6, 544, 761
TaaI ACNGT 2 cut(s) 1059, 1205
TaiI ACGT 4 cut(s) 141, 484, 1147, 1506
TasI AATT 9 cut(s) 32, 126, 357, 494, 675, 1018, 1196, 1467, 1488
TatI WGTACW 2 cut(s) 795, 1104
TfiI GAWTC 4 cut(s) 100, 540, 742, 1169
Tru1I TTAA 5 cut(s) 497, 1089, 1199, 1239, 1401
Tru9I TTAA 5 cut(s) 497, 1089, 1199, 1239, 1401
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 87
Tsp45I GTSAC 1 cut(s) 22
TspGWI ACGGA 1 cut(s) 735
TspRI CASTG 1 cut(s) 282
Van91I CCANNNNNTGG 1 cut(s) 1601
VneI GTGCAC 1 cut(s) 776
XagI CCTNNNNNAGG 1 cut(s) 323
XapI RAATTY 4 cut(s) 32, 675, 1018, 1467
XceI RCATGY 3 cut(s) 424, 1334, 1487
XmaJI CCTAGG 1 cut(s) 761
XspI CTAG 3 cut(s) 657, 762, 1235
ZrmI AGTACT 1 cut(s) 797
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.