Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001246

Physical Location & Seq

Genomic Coordinates Forward (+)

4940973 .. 4945783

Gene Structure 4811 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1043900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGAAGAAACCGCAAAAGATCACTCAGTACAGGGAGAGGCTGGACAGGACACTGGCATCTCCTAGTCTAAGTGATGAGGAAGCATTAAAAACCCTTGTCAAGAATCAGCTGATTCGTTCTTTGGAAAATGAAAATGAAGGATGCAATGAGAATATGGTTGAAGAAAAGACGGCTGAAGTATCCAATTTACTGGATATGTTAAGGAGCGCTTCCCTTATTAATGACAAGGGATTGAAAACCTGTGAGACCACAACCCAACCCGAATGGAAATTAAAACACGATAATGAAGAGTTCCGTGTGATGTATCGTGAGGGAATTGAAGGCAGTCCCTTTCATACATTACTTGCTGAAGGCTATGTAGACGGGCCTGTAGATGTCTGTTTATGCATCTCGTGGGAGTCAGACCTTTACAAGAAATGGTGGCCTGAGTCTACAGTTCCAACTTTCAAAATCCTCTCTAGCAAATGTTTGAAGAAGGTCAGGATCGGTGAACAGATATCTTTAGTGAGGATGAAAATTCCATGGCCACTTTCAGCTAGGGAGGCAGCTGTACACTATTTTATGTTTGAGTACTTCCAAGATGATCTGATTGTTGTGCTCTTTAAATCGATCTCCGACTTGGAAAGCATTGATGATACTCATTGTCCCACCAATGAGGCTACTGCTGGAGCAAAGGATGCTGTAAGGATAGATATGGTGGGTGGCTTTGCTTTGCAGAAGGTTACCAACGAAAGAAGTTACTTTCGGACAATATCAAGTACGGATATAAAGATGGATTTCGTCCCGCCATCCCTTTTAAATTTTATCTCGAGGCAGCTCATTGGCAATGGTCTCAAACTCTACCAAAAGGTAGTGTCTTCTAAGCTTAACTGCAATGACGATTACAGTAAGGCCTTGAGTGGCCCACTGTACTCTCATATACGTGAAGCTCTTTTTTCACATAATAAATCAAATGGGGCTTTGGAAGAAAAGAAGCTACATAATGATACATTCAGTCTCTCGGAAGAACATCTGGTAAAAGATAAGACGGATGAGAGTTTGGTAGATGTGGATCAGAAAGTCGATAATGATCACCCTGCAAGTGGAGTGGCTCCAGAAGTTGCAAAAGTTATACGAGGAGGCTCATTTGATGAGATTGAGGAGGTAGAGAGCGACGAAAGCAGGCGACATGAGGTTCAAACACCCAACAGAGTTGTTGAAAGGGAACGTGTAAATGGAAAGGGAAATGTTTTGGTCAGTTCTGAAGTGGAGCAAGCTCTAGGAACATTAGAAAAGGTAATTTATAAGGTTCGGCAAAATAGATTGAATGCTCAAAAGCGGTCGTCTTCTGGCTTCACCAATGGAATCCCGCCAAAGGAAAATAATGCAGGTAACCCAAAATCTTTAGAAGGTGGAGTTTGTGGAAGTGGTGAACATGTTCTTGAAGCATCTAAAGAAGAGTTTGTCAAGACGACTCAGCCCGAGTCTGCAAGGAATGGCTCTGTCATTCACAACTCAAGCAATGCAGGTTCGAATTCCTTGTCCAAGGATGTTAACCCTAACAGAGCACTACCTACTTCCCTGGAGCAGGAACTTTCGGTTTCTCATGACAGTAACCAGGTTGCATTGCTTTCCTCCAATGACGGAACTACAGAGACACCTGCTGTCGATCATACTATGCATCGTTCCGACCGCATGAACTCCGAGATCAACGGCGATCAAGAAAGTCGCCCTAGTAGAACAAAAAAGCCGAGGCAGCAAAAAGCGCTTCGGTTCTGTTGCTTCAGCATCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

592

Amino Acids

66.17

Weight (kDa)

5.73

Isoelectric Point (pI)

35.92

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001246)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G06440 AT5G06440 AT5G06440 AT5G06440 AT5G06440 AT5G06440
fragaria_vesca	FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770 FvH4_6g20770
malus_domestica	MD12G1043900.v1.1 MD14G1042900.v1.1
prunus_persica	Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1 Prupe.7G091200_v2.0.a1
pyrus_communis	pycom12g03770 pycom12g03780 pycom12g03790 pycom14g03640 pycom14g03650
rosa_chinensis	RchiOBHm_Chr3g0476671
rosa_laevigata	RLG00000001975 RLG00000023756
rosa_multiflora	Rmu_co8489431.1_g000001 Rmu_sc0008241.1_g000019 Rmu_sc0027136.1_g000001
rosa_roxburghii	Rroxscaffold_3G00235830 Rroxscaffold_6G00405100
rosa_rugosa	Rorug03G0156400 Rorug03G0156500 Rorug03G0156600
rosa_samantha	Rh3AG206800 Rh3BG239300 Rh3CG233200 Rh3DG233100 Rh3DG233200
rosa_wichuraiana	Rw3G018820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1284
AarI	CACCTGC	1 cut(s)	1648
Acc36I	ACCTGC	3 cut(s)	1358, 1496, 1648
AccI	GTMKAC	2 cut(s)	360, 431
AciI	CCGC	5 cut(s)	11, 785, 1318, 1349, 1672
AclWI	GGATC	2 cut(s)	491, 1059
AcoI	YGGCCR	1 cut(s)	524
AcsI	RAATTY	3 cut(s)	516, 799, 1513
AcuI	CTGAAG	4 cut(s)	195, 369, 1263, 1747
AfaI	GTAC	5 cut(s)	29, 552, 572, 760, 911
AfeI	AGCGCT	2 cut(s)	208, 1746
AfiI	CCNNNNNNNGG	3 cut(s)	1082, 1354, 1567
AflIII	ACRYGT	2 cut(s)	1207, 1414
AgsI	TTSAA	9 cut(s)	161, 235, 320, 448, 472, 1178, 1199, 1306, 1424
AjnI	CCWGG	2 cut(s)	1560, 1596
AluBI	AGCT	8 cut(s)	109, 536, 548, 817, 865, 929, 976, 1256
AluI	AGCT	8 cut(s)	109, 536, 548, 817, 865, 929, 976, 1256
Alw21I	GWGCWC	2 cut(s)	600, 1549
Alw26I	GTCTC	4 cut(s)	239, 836, 1001, 1628
AlwI	GGATC	2 cut(s)	491, 1059
Ama87I	CYCGRG	2 cut(s)	808, 1460
Aor51HI	AGCGCT	2 cut(s)	208, 1746
AoxI	GGCC	5 cut(s)	365, 422, 524, 891, 901
ApeKI	GCWGC	3 cut(s)	545, 814, 1735
ApoI	RAATTY	3 cut(s)	516, 799, 1513
ArsI	GACNNNNNNTTYG	2 cut(s)	1505, 1537
AseI	ATTAAT	1 cut(s)	219
Asp700I	GAANNNNTTC	1 cut(s)	1416
AspLEI	GCGC	2 cut(s)	209, 1747
AspS9I	GGNCC	2 cut(s)	365, 902
AsuHPI	GGTGA	4 cut(s)	500, 1064, 1327, 1421
AsuII	TTCGAA	1 cut(s)	1511
AvaI	CYCGRG	2 cut(s)	808, 1460
BalI	TGGCCA	1 cut(s)	526
BauI	CACGAG	1 cut(s)	391
BbsI	GAAGAC	2 cut(s)	849, 1317
Bbv12I	GWGCWC	2 cut(s)	600, 1549
BbvI	GCAGC	3 cut(s)	557, 826, 1747
BccI	CCATC	2 cut(s)	766, 796
BceAI	ACGGC	2 cut(s)	186, 1708
BciT130I	CCWGG	2 cut(s)	1562, 1598
BciVI	GTATCC	1 cut(s)	190
BclI	TGATCA	1 cut(s)	1069
BcoDI	GTCTC	4 cut(s)	239, 836, 1001, 1628
BfaI	CTAG	5 cut(s)	63, 459, 537, 1259, 1713
BfmI	CTRYAG	3 cut(s)	369, 432, 1629
BfoI	RGCGCY	2 cut(s)	210, 1748
BfuAI	ACCTGC	3 cut(s)	1358, 1496, 1648
BfuI	GTATCC	1 cut(s)	190
BisI	GCNGC	3 cut(s)	546, 815, 1736
BlsI	GCNGC	3 cut(s)	547, 816, 1737
BmcAI	AGTACT	1 cut(s)	572
Bme1390I	CCNGG	2 cut(s)	1562, 1598
BmeT110I	CYCGRG	2 cut(s)	808, 1460
BmgT120I	GGNCC	2 cut(s)	365, 902
BmiI	GGNNCC	1 cut(s)	1092
BmrFI	CCNGG	2 cut(s)	1562, 1598
BmsI	GCATC	6 cut(s)	65, 131, 396, 667, 1436, 1669
BpiI	GAAGAC	2 cut(s)	849, 1317
BpmI	CTGGAG	3 cut(s)	687, 1077, 1583
Bpu14I	TTCGAA	1 cut(s)	1511
BpuEI	CTTGAG	2 cut(s)	916, 1480
Bsa29I	ATCGAT	1 cut(s)	608
BsaAI	YACGTR	1 cut(s)	923
BsaBI	GATNNNNATC	2 cut(s)	1050, 1068
BsaI	GGTCTC	2 cut(s)	239, 836
BsaJI	CCNNGG	4 cut(s)	521, 1524, 1560, 1730
Bsc4I	CCNNNNNNNGG	3 cut(s)	1082, 1354, 1567
Bse1I	ACTGG	2 cut(s)	57, 195
Bse3DI	GCAATG	5 cut(s)	151, 832, 880, 1507, 1604
Bse8I	GATNNNNATC	2 cut(s)	1050, 1068
BseBI	CCWGG	2 cut(s)	1562, 1598
BseCI	ATCGAT	1 cut(s)	608
BseDI	CCNNGG	4 cut(s)	521, 1524, 1560, 1730
BseGI	GGATG	6 cut(s)	146, 516, 682, 788, 1036, 1534
BseJI	GATNNNNATC	2 cut(s)	1050, 1068
BseLI	CCNNNNNNNGG	3 cut(s)	1082, 1354, 1567
BseMI	GCAATG	5 cut(s)	151, 832, 880, 1507, 1604
BseMII	CTCAG	3 cut(s)	38, 417, 1469
BseNI	ACTGG	2 cut(s)	57, 195
BseRI	GAGGAG	2 cut(s)	1131, 1154
BseXI	GCAGC	3 cut(s)	557, 826, 1747
Bsh1285I	CGRYCG	2 cut(s)	1322, 1672
BshFI	GGCC	5 cut(s)	367, 424, 526, 893, 903
BshVI	ATCGAT	1 cut(s)	608
BsiEI	CGRYCG	2 cut(s)	1322, 1672
BsiHKAI	GWGCWC	2 cut(s)	600, 1549
BsiHKCI	CYCGRG	2 cut(s)	808, 1460
BslFI	GGGAC	3 cut(s)	312, 630, 767
BslI	CCNNNNNNNGG	3 cut(s)	1082, 1354, 1567
BsmAI	GTCTC	4 cut(s)	239, 836, 1001, 1628
BsmFI	GGGAC	3 cut(s)	312, 630, 767
BsmI	GAATGC	1 cut(s)	1312
BsnI	GGCC	5 cut(s)	367, 424, 526, 893, 903
Bso31I	GGTCTC	2 cut(s)	239, 836
BsoBI	CYCGRG	2 cut(s)	808, 1460
Bsp119I	TTCGAA	1 cut(s)	1511
Bsp1286I	GDGCHC	2 cut(s)	600, 1549
Bsp1407I	TGTACA	1 cut(s)	550
Bsp143I	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
Bsp19I	CCATGG	1 cut(s)	521
BspACI	CCGC	5 cut(s)	11, 785, 1318, 1349, 1672
BspANI	GGCC	5 cut(s)	367, 424, 526, 893, 903
BspCNI	CTCAG	3 cut(s)	37, 418, 1468
BspDI	ATCGAT	1 cut(s)	608
BspHI	TCATGA	1 cut(s)	1585
BspLI	GGNNCC	1 cut(s)	1092
BspMI	ACCTGC	3 cut(s)	1358, 1496, 1648
BspPI	GGATC	2 cut(s)	491, 1059
BspT104I	TTCGAA	1 cut(s)	1511
BspTNI	GGTCTC	2 cut(s)	239, 836
BsrDI	GCAATG	5 cut(s)	151, 832, 880, 1507, 1604
BsrGI	TGTACA	1 cut(s)	550
BsrI	ACTGG	2 cut(s)	57, 195
BssECI	CCNNGG	4 cut(s)	521, 1524, 1560, 1730
BssMI	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
BssSI	CACGAG	1 cut(s)	391
BssT1I	CCWWGG	2 cut(s)	521, 1524
Bst2BI	CACGAG	1 cut(s)	391
Bst2UI	CCWGG	2 cut(s)	1562, 1598
Bst4CI	ACNGT	4 cut(s)	436, 887, 909, 1592
Bst6I	CTCTTC	2 cut(s)	282, 1431
BstAPI	GCANNNNNTGC	1 cut(s)	677
BstAUI	TGTACA	1 cut(s)	550
BstBAI	YACGTR	1 cut(s)	923
BstBI	TTCGAA	1 cut(s)	1511
BstC8I	GCNNGC	2 cut(s)	1163, 1254
BstDEI	CTNAG	5 cut(s)	24, 68, 426, 861, 1455
BstDSI	CCRYGG	1 cut(s)	521
BstEII	GGTNACC	2 cut(s)	721, 1370
BstENI	CCTNNNNNAGG	1 cut(s)	1565
BstF5I	GGATG	6 cut(s)	146, 516, 682, 788, 1036, 1534
BstH2I	RGCGCY	2 cut(s)	210, 1748
BstHHI	GCGC	2 cut(s)	209, 1747
BstKTI	GATC	9 cut(s)	21, 486, 586, 612, 1054, 1072, 1651, 1689, 1699
BstMAI	GTCTC	4 cut(s)	239, 836, 1001, 1628
BstMBI	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
BstMCI	CGRYCG	2 cut(s)	1322, 1672
BstMWI	GCNNNNNNNGC	5 cut(s)	542, 677, 1158, 1735, 1744
BstNI	CCWGG	2 cut(s)	1562, 1598
BstNSI	RCATGY	1 cut(s)	1418
BstPI	GGTNACC	2 cut(s)	721, 1370
BstSCI	CCNGG	2 cut(s)	1560, 1596
BstSFI	CTRYAG	3 cut(s)	369, 432, 1629
BstV1I	GCAGC	3 cut(s)	557, 826, 1747
BstV2I	GAAGAC	2 cut(s)	849, 1317
BstXI	CCANNNNNNTGG	1 cut(s)	190
Bsu15I	ATCGAT	1 cut(s)	608
BsuI	GTATCC	1 cut(s)	190
BsuRI	GGCC	5 cut(s)	367, 424, 526, 893, 903
BsuTUI	ATCGAT	1 cut(s)	608
BtgI	CCRYGG	1 cut(s)	521
BtsCI	GGATG	6 cut(s)	146, 516, 682, 788, 1036, 1534
BtsIMutI	CAGTG	2 cut(s)	50, 905
BveI	ACCTGC	3 cut(s)	1358, 1496, 1648
Cac8I	GCNNGC	2 cut(s)	1163, 1254
CciI	TCATGA	1 cut(s)	1585
CfoI	GCGC	2 cut(s)	209, 1747
Cfr13I	GGNCC	2 cut(s)	365, 902
ClaI	ATCGAT	1 cut(s)	608
CsiI	ACCWGGT	1 cut(s)	1596
Csp6I	GTAC	5 cut(s)	28, 551, 571, 759, 910
CspCI	CAANNNNNGTGG	2 cut(s)	1068, 1103
CviAII	CATG	5 cut(s)	522, 1169, 1415, 1586, 1675
CviQI	GTAC	5 cut(s)	28, 551, 571, 759, 910
DdeI	CTNAG	5 cut(s)	24, 68, 426, 861, 1455
DpnI	GATC	9 cut(s)	20, 485, 585, 611, 1053, 1071, 1650, 1688, 1698
DpnII	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
DraI	TTTAAA	2 cut(s)	604, 798
EaeI	YGGCCR	1 cut(s)	524
Eam1104I	CTCTTC	2 cut(s)	282, 1431
EarI	CTCTTC	2 cut(s)	282, 1431
Eco130I	CCWWGG	2 cut(s)	521, 1524
Eco147I	AGGCCT	1 cut(s)	893
Eco31I	GGTCTC	2 cut(s)	239, 836
Eco32I	GATATC	1 cut(s)	498
Eco47III	AGCGCT	2 cut(s)	208, 1746
Eco57I	CTGAAG	4 cut(s)	195, 369, 1263, 1747
Eco88I	CYCGRG	2 cut(s)	808, 1460
Eco91I	GGTNACC	2 cut(s)	721, 1370
EcoNI	CCTNNNNNAGG	1 cut(s)	1565
EcoO65I	GGTNACC	2 cut(s)	721, 1370
EcoRI	GAATTC	1 cut(s)	1513
EcoRII	CCWGG	2 cut(s)	1560, 1596
EcoRV	GATATC	1 cut(s)	498
EcoT14I	CCWWGG	2 cut(s)	521, 1524
EcoT22I	ATGCAT	2 cut(s)	389, 1662
ErhI	CCWWGG	2 cut(s)	521, 1524
FaeI	CATG	5 cut(s)	525, 1172, 1418, 1589, 1678
FalI	AAGNNNNNCTT	2 cut(s)	193, 225
FaqI	GGGAC	3 cut(s)	312, 630, 767
FatI	CATG	5 cut(s)	521, 1168, 1414, 1585, 1674
FauI	CCCGC	2 cut(s)	792, 1356
FbaI	TGATCA	1 cut(s)	1069
FblI	GTMKAC	2 cut(s)	360, 431
Fnu4HI	GCNGC	3 cut(s)	546, 815, 1736
FokI	GGATG	6 cut(s)	153, 523, 689, 775, 1043, 1541
Fsp4HI	GCNGC	3 cut(s)	546, 815, 1736
FspBI	CTAG	5 cut(s)	63, 459, 537, 1259, 1713
GlaI	GCGC	2 cut(s)	208, 1746
GluI	GCNGC	3 cut(s)	546, 815, 1736
GsuI	CTGGAG	3 cut(s)	687, 1077, 1583
HaeII	RGCGCY	2 cut(s)	210, 1748
HaeIII	GGCC	5 cut(s)	367, 424, 526, 893, 903
HhaI	GCGC	2 cut(s)	209, 1747
Hin1II	CATG	5 cut(s)	525, 1172, 1418, 1589, 1678
Hin6I	GCGC	2 cut(s)	207, 1745
HinP1I	GCGC	2 cut(s)	207, 1745
HincII	GTYRAC	1 cut(s)	1534
HindII	GTYRAC	1 cut(s)	1534
HindIII	AAGCTT	1 cut(s)	863
HinfI	GANTC	7 cut(s)	103, 112, 398, 428, 1344, 1453, 1463
HpaI	GTTAAC	1 cut(s)	1534
HphI	GGTGA	4 cut(s)	500, 1064, 1327, 1421
Hpy166II	GTNNAC	6 cut(s)	361, 432, 491, 553, 1412, 1534
Hpy188I	TCNGA	9 cut(s)	403, 588, 616, 747, 1003, 1056, 1243, 1669, 1684
Hpy188III	TCNNGA	9 cut(s)	100, 308, 481, 808, 1094, 1421, 1447, 1586, 1700
Hpy8I	GTNNAC	6 cut(s)	361, 432, 491, 553, 1412, 1534
Hpy99I	CGWCG	1 cut(s)	1157
HpyAV	CCTTC	6 cut(s)	131, 314, 344, 469, 712, 1382
HpyCH4III	ACNGT	4 cut(s)	436, 887, 909, 1592
HpyCH4IV	ACGT	2 cut(s)	922, 1207
HpyF10VI	GCNNNNNNNGC	5 cut(s)	542, 677, 1158, 1735, 1744
HpyF3I	CTNAG	5 cut(s)	24, 68, 426, 861, 1455
HpySE526I	ACGT	2 cut(s)	922, 1207
Hsp92II	CATG	5 cut(s)	525, 1172, 1418, 1589, 1678
HspAI	GCGC	2 cut(s)	207, 1745
Ksp22I	TGATCA	1 cut(s)	1069
KspAI	GTTAAC	1 cut(s)	1534
Kzo9I	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
LmnI	GCTCC	5 cut(s)	204, 668, 1096, 1249, 1564
Lsp1109I	GCAGC	3 cut(s)	557, 826, 1747
LweI	GCATC	6 cut(s)	65, 131, 396, 667, 1436, 1669
MabI	ACCWGGT	1 cut(s)	1596
MaeI	CTAG	5 cut(s)	63, 459, 537, 1259, 1713
MaeII	ACGT	2 cut(s)	922, 1207
MaeIII	GTNAC	4 cut(s)	721, 737, 1370, 1592
MalI	GATC	9 cut(s)	20, 485, 585, 611, 1053, 1071, 1650, 1688, 1698
MboI	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
MboII	GAAGA	9 cut(s)	16, 173, 299, 484, 849, 977, 1016, 1317, 1448
MhlI	GDGCHC	2 cut(s)	600, 1549
MlsI	TGGCCA	1 cut(s)	526
MluCI	AATT	7 cut(s)	184, 269, 315, 516, 799, 1278, 1513
MluNI	TGGCCA	1 cut(s)	526
MlyI	GAGTC	4 cut(s)	407, 437, 1447, 1472
MmeI	TCCRAC	3 cut(s)	464, 639, 1692
Mox20I	TGGCCA	1 cut(s)	526
Mph1103I	ATGCAT	2 cut(s)	389, 1662
MroXI	GAANNNNTTC	1 cut(s)	1416
MscI	TGGCCA	1 cut(s)	526
MseI	TTAA	9 cut(s)	86, 200, 219, 272, 603, 797, 867, 1533, 1774
MslI	CAYNNNNRTG	2 cut(s)	282, 921
Msp20I	TGGCCA	1 cut(s)	526
MspA1I	CMGCKG	2 cut(s)	109, 548
MspR9I	CCNGG	2 cut(s)	1562, 1598
Mva1269I	GAATGC	1 cut(s)	1312
MvaI	CCWGG	2 cut(s)	1562, 1598
MwoI	GCNNNNNNNGC	5 cut(s)	542, 677, 1158, 1735, 1744
NcoI	CCATGG	1 cut(s)	521
NdeII	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
NlaIII	CATG	5 cut(s)	525, 1172, 1418, 1589, 1678
NlaIV	GGNNCC	1 cut(s)	1092
NmeAIII	GCCGAG	1 cut(s)	1755
NsiI	ATGCAT	2 cut(s)	389, 1662
NspI	RCATGY	1 cut(s)	1418
NspV	TTCGAA	1 cut(s)	1511
PaeR7I	CTCGAG	1 cut(s)	808
PagI	TCATGA	1 cut(s)	1585
PaqCI	CACCTGC	1 cut(s)	1648
PceI	AGGCCT	1 cut(s)	893
PciI	ACATGT	1 cut(s)	1414
PctI	GAATGC	1 cut(s)	1312
PdmI	GAANNNNTTC	1 cut(s)	1416
PfeI	GAWTC	3 cut(s)	103, 112, 1344
PkrI	GCNGC	3 cut(s)	547, 816, 1737
PleI	GAGTC	4 cut(s)	406, 436, 1447, 1471
PpsI	GAGTC	4 cut(s)	406, 436, 1447, 1471
Ppu21I	YACGTR	1 cut(s)	923
PscI	ACATGT	1 cut(s)	1414
PshBI	ATTAAT	1 cut(s)	219
PsiI	TTATAA	1 cut(s)	1284
Psp6I	CCWGG	2 cut(s)	1560, 1596
PspEI	GGTNACC	2 cut(s)	721, 1370
PspGI	CCWGG	2 cut(s)	1560, 1596
PspN4I	GGNNCC	1 cut(s)	1092
PspPI	GGNCC	2 cut(s)	365, 902
PvuII	CAGCTG	2 cut(s)	109, 548
RsaI	GTAC	5 cut(s)	29, 552, 572, 760, 911
RsaNI	GTAC	5 cut(s)	28, 551, 571, 759, 910
RseI	CAYNNNNRTG	2 cut(s)	282, 921
SaqAI	TTAA	9 cut(s)	86, 200, 219, 272, 603, 797, 867, 1533, 1774
SatI	GCNGC	3 cut(s)	546, 815, 1736
Sau3AI	GATC	9 cut(s)	18, 483, 583, 609, 1051, 1069, 1648, 1686, 1696
Sau96I	GGNCC	2 cut(s)	365, 902
ScaI	AGTACT	1 cut(s)	572
SchI	GAGTC	4 cut(s)	407, 437, 1447, 1472
ScrFI	CCNGG	2 cut(s)	1562, 1598
SduI	GDGCHC	2 cut(s)	600, 1549
SexAI	ACCWGGT	1 cut(s)	1596
SfaNI	GCATC	6 cut(s)	65, 131, 396, 667, 1436, 1669
SfcI	CTRYAG	3 cut(s)	369, 432, 1629
Sfr274I	CTCGAG	1 cut(s)	808
SfuI	TTCGAA	1 cut(s)	1511
SlaI	CTCGAG	1 cut(s)	808
SmiMI	CAYNNNNRTG	2 cut(s)	282, 921
SmlI	CTYRAG	3 cut(s)	808, 895, 1495
SmoI	CTYRAG	3 cut(s)	808, 895, 1495
Sse9I	AATT	7 cut(s)	184, 269, 315, 516, 799, 1278, 1513
SseBI	AGGCCT	1 cut(s)	893
SsiI	CCGC	5 cut(s)	11, 785, 1318, 1349, 1672
SspMI	CTAG	5 cut(s)	63, 459, 537, 1259, 1713
StuI	AGGCCT	1 cut(s)	893
StyD4I	CCNGG	2 cut(s)	1560, 1596
StyI	CCWWGG	2 cut(s)	521, 1524
TaaI	ACNGT	4 cut(s)	436, 887, 909, 1592
TaiI	ACGT	2 cut(s)	925, 1210
TaqI	TCGA	5 cut(s)	608, 809, 1062, 1511, 1647
TasI	AATT	7 cut(s)	184, 269, 315, 516, 799, 1278, 1513
TatI	WGTACW	4 cut(s)	27, 550, 570, 909
TfiI	GAWTC	3 cut(s)	103, 112, 1344
Tru1I	TTAA	9 cut(s)	86, 200, 219, 272, 603, 797, 867, 1533, 1774
Tru9I	TTAA	9 cut(s)	86, 200, 219, 272, 603, 797, 867, 1533, 1774
TscAI	CASTG	2 cut(s)	57, 912
TseI	GCWGC	3 cut(s)	545, 814, 1735
TspDTI	ATGAA	7 cut(s)	17, 144, 150, 300, 323, 527, 1691
TspGWI	ACGGA	4 cut(s)	284, 776, 1043, 1638
TspRI	CASTG	2 cut(s)	57, 912
VspI	ATTAAT	1 cut(s)	219
XagI	CCTNNNNNAGG	1 cut(s)	1565
XapI	RAATTY	3 cut(s)	516, 799, 1513
XceI	RCATGY	1 cut(s)	1418
XhoI	CTCGAG	1 cut(s)	808
XmiI	GTMKAC	2 cut(s)	360, 431
XmnI	GAANNNNTTC	1 cut(s)	1416
XspI	CTAG	5 cut(s)	63, 459, 537, 1259, 1713
ZrmI	AGTACT	1 cut(s)	572
Zsp2I	ATGCAT	2 cut(s)	389, 1662

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.