pycom12g03770

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001246
Physical Location & Seq
Forward (+)
3668266 .. 3669915
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03770.2

Sequence Viewer

Length: 543 bp
ATGGAGAAACCGCGAAAGATCATTCAGTACAGGGAGAGGCTGGACAGGACACTGGCATCTCCTAGTCTAAGTGATGAGGAAGCATTGAAAACCCTTGTCAAGAATCAGCTGATTCGTTCTTTGGAAAATGAAAATGAAGGATGCAATGAGAATATGGTTGAAGAAAAGACGGCTGAAGTATCCAATTTACTGGATATGTTAAGGAGTGCTTCCCTTGTTAATGACAAGGGATTGAAAACCTGTGAGACCACAACCCAACCCGAATGGAAATTAAAACAGGATAATGAAGAGTTCCGTGTGATGTATCGTGAGGGAATTGAAGGCAGTCCCTTTCATACATTACTTGCTGAAGGCTATGTAGATGGGCCTGTGGATGTCTGTTTATGCATCTCGTGGGAGTCAGACCTTTACAAGAAATGGTGGCCTCAGTCTACAGTTCCAACTTTCAAAATCCTCTCTAGCAAATGTTTGCAGAAGGTCAGGATCGGTGAACAGATATCTTTAGTGAGAAAGGATGAAAATTCCATGGCCACTTTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.74

Weight (kDa)

5.15

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 431
AccII CGCG 1 cut(s) 13
AciI CCGC 1 cut(s) 11
AclWI GGATC 1 cut(s) 491
AcoI YGGCCR 1 cut(s) 528
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 2 cut(s) 195, 369
AfaI GTAC 1 cut(s) 29
AgsI TTSAA 5 cut(s) 88, 161, 235, 320, 448
AluBI AGCT 2 cut(s) 109, 540
AluI AGCT 2 cut(s) 109, 540
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 491
AoxI GGCC 3 cut(s) 365, 422, 528
ApoI RAATTY 1 cut(s) 520
AspS9I GGNCC 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 500
BalI TGGCCA 1 cut(s) 530
BauI CACGAG 1 cut(s) 391
BccI CCATC 1 cut(s) 356
BceAI ACGGC 1 cut(s) 186
BciVI GTATCC 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 3 cut(s) 63, 459, 541
BfmI CTRYAG 1 cut(s) 432
BfuI GTATCC 1 cut(s) 190
BmgT120I GGNCC 1 cut(s) 365
BmsI GCATC 3 cut(s) 65, 131, 396
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 525
Bse1I ACTGG 2 cut(s) 57, 195
Bse3DI GCAATG 1 cut(s) 151
BseDI CCNNGG 1 cut(s) 525
BseGI GGATG 3 cut(s) 146, 379, 520
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 440
BseNI ACTGG 2 cut(s) 57, 195
Bsh1236I CGCG 1 cut(s) 13
BshFI GGCC 3 cut(s) 367, 424, 530
BslFI GGGAC 1 cut(s) 312
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 312
BsnI GGCC 3 cut(s) 367, 424, 530
Bso31I GGTCTC 1 cut(s) 239
Bsp143I GATC 2 cut(s) 18, 483
Bsp19I CCATGG 1 cut(s) 525
BspACI CCGC 1 cut(s) 11
BspANI GGCC 3 cut(s) 367, 424, 530
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 1 cut(s) 13
BspPI GGATC 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 151
BsrI ACTGG 2 cut(s) 57, 195
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 2 cut(s) 18, 483
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 391
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 282
BstDEI CTNAG 2 cut(s) 68, 426
BstDSI CCRYGG 1 cut(s) 525
BstF5I GGATG 3 cut(s) 146, 379, 520
BstFNI CGCG 1 cut(s) 13
BstKTI GATC 2 cut(s) 21, 486
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 18, 483
BstSFI CTRYAG 1 cut(s) 432
BstUI CGCG 1 cut(s) 13
BstXI CCANNNNNNTGG 1 cut(s) 190
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 3 cut(s) 367, 424, 530
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 3 cut(s) 146, 379, 520
BtsIMutI CAGTG 1 cut(s) 50
Cfr13I GGNCC 1 cut(s) 365
Csp6I GTAC 1 cut(s) 28
CviAII CATG 1 cut(s) 526
CviJI RGCY 8 cut(s) 40, 109, 173, 354, 367, 424, 530, 540
CviKI_1 RGCY 8 cut(s) 40, 109, 173, 354, 367, 424, 530, 540
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 2 cut(s) 68, 426
DpnI GATC 2 cut(s) 20, 485
DpnII GATC 2 cut(s) 18, 483
EaeI YGGCCR 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 525
Eco31I GGTCTC 1 cut(s) 239
Eco32I GATATC 1 cut(s) 498
Eco57I CTGAAG 2 cut(s) 195, 369
EcoRV GATATC 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 525
EcoT22I ATGCAT 1 cut(s) 389
ErhI CCWWGG 1 cut(s) 525
FaeI CATG 1 cut(s) 529
FaiI YATR 6 cut(s) 155, 197, 336, 357, 385, 527
FalI AAGNNNNNCTT 2 cut(s) 193, 225
FaqI GGGAC 1 cut(s) 312
FatI CATG 1 cut(s) 525
FblI GTMKAC 1 cut(s) 431
FokI GGATG 3 cut(s) 153, 386, 527
FspBI CTAG 3 cut(s) 63, 459, 541
HaeIII GGCC 3 cut(s) 367, 424, 530
Hin1II CATG 1 cut(s) 529
HinfI GANTC 3 cut(s) 103, 112, 398
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 2 cut(s) 432, 491
Hpy188I TCNGA 1 cut(s) 403
Hpy188III TCNNGA 3 cut(s) 100, 308, 481
Hpy8I GTNNAC 2 cut(s) 432, 491
HpyAV CCTTC 4 cut(s) 131, 314, 344, 469
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4V TGCA 3 cut(s) 144, 387, 472
HpyF3I CTNAG 2 cut(s) 68, 426
Hsp92II CATG 1 cut(s) 529
Kzo9I GATC 2 cut(s) 18, 483
LpnPI CCDG 9 cut(s) 16, 26, 31, 38, 176, 253, 263, 381, 466
LweI GCATC 3 cut(s) 65, 131, 396
MaeI CTAG 3 cut(s) 63, 459, 541
MalI GATC 2 cut(s) 20, 485
MboI GATC 2 cut(s) 18, 483
MboII GAAGA 2 cut(s) 173, 299
MlsI TGGCCA 1 cut(s) 530
MluCI AATT 4 cut(s) 184, 269, 315, 520
MluNI TGGCCA 1 cut(s) 530
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 5 cut(s) 30, 70, 304, 435, 464
Mox20I TGGCCA 1 cut(s) 530
Mph1103I ATGCAT 1 cut(s) 389
MscI TGGCCA 1 cut(s) 530
MseI TTAA 3 cut(s) 200, 219, 272
Msp20I TGGCCA 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 109
MvnI CGCG 1 cut(s) 13
NcoI CCATGG 1 cut(s) 525
NdeII GATC 2 cut(s) 18, 483
NlaIII CATG 1 cut(s) 529
NsiI ATGCAT 1 cut(s) 389
PfeI GAWTC 2 cut(s) 103, 112
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PspPI GGNCC 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 109
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
SaqAI TTAA 3 cut(s) 200, 219, 272
Sau3AI GATC 2 cut(s) 18, 483
Sau96I GGNCC 1 cut(s) 365
SchI GAGTC 1 cut(s) 407
SetI ASST 5 cut(s) 111, 242, 408, 480, 542
SfaNI GCATC 3 cut(s) 65, 131, 396
SfcI CTRYAG 1 cut(s) 432
Sse9I AATT 4 cut(s) 184, 269, 315, 520
SsiI CCGC 1 cut(s) 11
SspMI CTAG 3 cut(s) 63, 459, 541
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 1 cut(s) 436
TasI AATT 4 cut(s) 184, 269, 315, 520
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 2 cut(s) 103, 112
Tru1I TTAA 3 cut(s) 200, 219, 272
Tru9I TTAA 3 cut(s) 200, 219, 272
TscAI CASTG 1 cut(s) 57
TspDTI ATGAA 5 cut(s) 144, 150, 300, 323, 531
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 57
XapI RAATTY 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 3 cut(s) 63, 459, 541
Zsp2I ATGCAT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.