FvH4_6g29540

CUE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003445
Physical Location & Seq
Reverse (-)
22805310 .. 22808279
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29540.t1

Sequence Viewer

Length: 783 bp
ATGTCTGCGGCAGTGTGCGGAAGCAAGAGGTCCTTCTTCGAAGAGCTTCCTCCGTCGCCTCCGCTCACTAAGAGACTCCGCTGCTCCTCTTCTACCTCCCCCGTCCGATTCTCTTCTCCTTATCTTATCGATCAACTCCGCTCCGTCTTCCCCCACATGGACCCTCAGATTCTTGAGCGAGCATTGGAAGAGTGTGGTAATGATATTGATGCTGCTATCAAGAGACTGAATGAGCTTCACATTGGATATGGGGATGAAAATGCCGCCCCTGCTGCGGAGCCGAATGATATTGTACAGAAAGGGATATTAACGGATGAGGAAGCGGCTGCTGCTTCTGAGAATACATCAGCTCCAAGCAACCTTCCTGCAGATGGTGCGGAATGGGTTGAATTATTTGTCAGGGAAATGATGAGTGCCACTAGCGTGGATGATGCTAGGGGACGTGCTGCAAAAGTGCTAGAGGTTTTAGAGAAGTCCATCAGTTCACGTGCTGGTGCGGAAGCAGCCCAAAATTTTCAAAAGGAGAATATGCTACTGAAAGAACAAATTGAAGGACTGATAAGGGACAATTCTGTCCTCAAACGTGCTGTGTCTATTCAGCATGAACGTCAGAAAGAGTATGAGGGCAGAAACCAAGAATTTCAGCATCTGAAACAGTTAGTAACTCAATACCAGGAACAGATGAGGACACTTGAGCTCAACAACTATGCCTTGACAATGCATCTGAAGCAGGCACAACAAGGCAACTCCATTCCTGGACGTTTCCATCCTGATGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

261

Amino Acids

29.04

Weight (kDa)

5.28

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 43 - 79 4.1e-09 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 572
AccBSI CCGCTC 2 cut(s) 64, 141
AcsI RAATTY 2 cut(s) 511, 638
AcuI CTGAAG 1 cut(s) 746
AcvI CACGTG 1 cut(s) 488
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 3 cut(s) 157, 274, 371
AgsI TTSAA 3 cut(s) 389, 518, 551
AjiI CACGTC 1 cut(s) 443
AjnI CCWGG 2 cut(s) 672, 754
AleI CACNNNNGTG 1 cut(s) 422
AluBI AGCT 4 cut(s) 46, 235, 350, 697
AluI AGCT 4 cut(s) 46, 235, 350, 697
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 2 cut(s) 67, 217
AlwNI CAGNNNCTG 1 cut(s) 649
ApeKI GCWGC 7 cut(s) 81, 212, 272, 326, 329, 446, 503
ApoI RAATTY 2 cut(s) 511, 638
Asp700I GAANNNNTTC 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 30, 160
AsuII TTCGAA 1 cut(s) 39
AvaII GGWCC 2 cut(s) 30, 160
BanII GRGCYC 1 cut(s) 699
BbrPI CACGTG 1 cut(s) 488
BbsI GAAGAC 2 cut(s) 139, 769
Bbv12I GWGCWC 1 cut(s) 699
BbvI GCAGC 7 cut(s) 68, 199, 259, 313, 316, 433, 515
BccI CCATC 3 cut(s) 365, 485, 774
BciT130I CCWGG 2 cut(s) 674, 756
BcoDI GTCTC 2 cut(s) 67, 217
BfaI CTAG 3 cut(s) 420, 435, 458
BfmI CTRYAG 1 cut(s) 366
Bme1390I CCNGG 2 cut(s) 674, 756
Bme18I GGWCC 2 cut(s) 30, 160
BmgBI CACGTC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 30, 160
BmiI GGNNCC 2 cut(s) 162, 279
BmrFI CCNGG 2 cut(s) 674, 756
BmsI GCATC 4 cut(s) 199, 421, 655, 730
BpiI GAAGAC 2 cut(s) 139, 769
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 194, 713
Bsa29I ATCGAT 1 cut(s) 129
BsaAI YACGTR 1 cut(s) 488
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 274, 371
BseBI CCWGG 2 cut(s) 674, 756
BseCI ATCGAT 1 cut(s) 129
BseGI GGATG 4 cut(s) 259, 319, 433, 766
BseLI CCNNNNNNNGG 3 cut(s) 157, 274, 371
BseMII CTCAG 2 cut(s) 179, 327
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 7 cut(s) 68, 199, 259, 313, 316, 433, 515
BshVI ATCGAT 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 699
BslFI GGGAC 2 cut(s) 453, 578
BslI CCNNNNNNNGG 3 cut(s) 157, 274, 371
BsmAI GTCTC 2 cut(s) 67, 217
BsmFI GGGAC 2 cut(s) 453, 578
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 699
Bsp1407I TGTACA 1 cut(s) 292
Bsp143I GATC 1 cut(s) 130
BspCNI CTCAG 2 cut(s) 178, 328
BspDI ATCGAT 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 162, 279
BspMAI CTGCAG 1 cut(s) 370
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BsrBI CCGCTC 2 cut(s) 64, 141
BsrGI TGTACA 1 cut(s) 292
BssMI GATC 1 cut(s) 130
Bst2UI CCWGG 2 cut(s) 674, 756
Bst4CI ACNGT 1 cut(s) 657
Bst6I CTCTTC 4 cut(s) 36, 94, 118, 183
BstAPI GCANNNNNTGC 1 cut(s) 374
BstAUI TGTACA 1 cut(s) 292
BstBAI YACGTR 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 180, 732
BstDEI CTNAG 3 cut(s) 69, 165, 336
BstF5I GGATG 4 cut(s) 259, 319, 433, 766
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 2 cut(s) 67, 217
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 6 cut(s) 269, 272, 329, 374, 503, 727
BstNI CCWGG 2 cut(s) 674, 756
BstSCI CCNGG 2 cut(s) 672, 754
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 7 cut(s) 68, 199, 259, 313, 316, 433, 515
BstV2I GAAGAC 2 cut(s) 139, 769
BstXI CCANNNNNNTGG 1 cut(s) 424
Bsu15I ATCGAT 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 129
BtrI CACGTC 1 cut(s) 443
BtsCI GGATG 4 cut(s) 259, 319, 433, 766
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 180, 732
CaiI CAGNNNCTG 1 cut(s) 649
Cfr13I GGNCC 2 cut(s) 30, 160
ClaI ATCGAT 1 cut(s) 129
Csp6I GTAC 1 cut(s) 293
CviAII CATG 2 cut(s) 157, 602
CviJI RGCY 7 cut(s) 46, 235, 280, 326, 350, 506, 697
CviKI_1 RGCY 7 cut(s) 46, 235, 280, 326, 350, 506, 697
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 3 cut(s) 69, 165, 336
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
Eam1104I CTCTTC 4 cut(s) 36, 94, 118, 183
EarI CTCTTC 4 cut(s) 36, 94, 118, 183
Ecl136II GAGCTC 1 cut(s) 697
Eco24I GRGCYC 1 cut(s) 699
Eco47I GGWCC 2 cut(s) 30, 160
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 746
Eco72I CACGTG 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 672, 754
EcoT22I ATGCAT 1 cut(s) 723
EcoT38I GRGCYC 1 cut(s) 699
FaeI CATG 2 cut(s) 160, 605
FaiI YATR 6 cut(s) 158, 249, 530, 603, 621, 708
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 2 cut(s) 453, 578
FatI CATG 2 cut(s) 156, 601
FokI GGATG 4 cut(s) 266, 326, 440, 753
FriOI GRGCYC 1 cut(s) 699
FspBI CTAG 3 cut(s) 420, 435, 458
Hin1II CATG 2 cut(s) 160, 605
HinfI GANTC 3 cut(s) 75, 108, 169
Hpy166II GTNNAC 1 cut(s) 485
Hpy188I TCNGA 6 cut(s) 107, 168, 337, 612, 651, 726
Hpy188III TCNNGA 3 cut(s) 173, 220, 770
Hpy8I GTNNAC 1 cut(s) 485
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 43, 371, 545
HpyCH4III ACNGT 1 cut(s) 657
HpyCH4IV ACGT 5 cut(s) 442, 487, 583, 607, 760
HpyCH4V TGCA 3 cut(s) 368, 449, 721
HpyF10VI GCNNNNNNNGC 6 cut(s) 269, 272, 329, 374, 503, 727
HpyF3I CTNAG 3 cut(s) 69, 165, 336
HpySE526I ACGT 5 cut(s) 442, 487, 583, 607, 760
Hsp92II CATG 2 cut(s) 160, 605
Kzo9I GATC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 89, 146, 277, 355
LpnPI CCDG 9 cut(s) 282, 378, 385, 477, 659, 686, 716, 741, 768
Lsp1109I GCAGC 7 cut(s) 68, 199, 259, 313, 316, 433, 515
LweI GCATC 4 cut(s) 199, 421, 655, 730
MaeI CTAG 3 cut(s) 420, 435, 458
MaeII ACGT 5 cut(s) 442, 487, 583, 607, 760
MaeIII GTNAC 1 cut(s) 661
MalI GATC 1 cut(s) 132
MbiI CCGCTC 2 cut(s) 64, 141
MboI GATC 1 cut(s) 130
MboII GAAGA 7 cut(s) 28, 53, 81, 105, 139, 200, 769
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 5 cut(s) 389, 511, 546, 568, 638
MlyI GAGTC 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 723
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 2 cut(s) 422, 771
MspA1I CMGCKG 1 cut(s) 81
MspR9I CCNGG 2 cut(s) 674, 756
MvaI CCWGG 2 cut(s) 674, 756
MwoI GCNNNNNNNGC 6 cut(s) 269, 272, 329, 374, 503, 727
NdeII GATC 1 cut(s) 130
NlaIII CATG 2 cut(s) 160, 605
NlaIV GGNNCC 2 cut(s) 162, 279
NsiI ATGCAT 1 cut(s) 723
NspV TTCGAA 1 cut(s) 39
OliI CACNNNNGTG 1 cut(s) 422
PciSI GCTCTTC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 108, 169
PfoI TCCNGGA 1 cut(s) 754
PleI GAGTC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 488
PmlI CACGTG 1 cut(s) 488
PpsI GAGTC 1 cut(s) 69
Ppu21I YACGTR 1 cut(s) 488
PpuMI RGGWCCY 1 cut(s) 30
Psp124BI GAGCTC 1 cut(s) 699
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 2 cut(s) 672, 754
PspCI CACGTG 1 cut(s) 488
PspGI CCWGG 2 cut(s) 672, 754
PspN4I GGNNCC 2 cut(s) 162, 279
PspPI GGNCC 2 cut(s) 30, 160
PspPPI RGGWCCY 1 cut(s) 30
PstI CTGCAG 1 cut(s) 370
PstNI CAGNNNCTG 1 cut(s) 649
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
RseI CAYNNNNRTG 2 cut(s) 422, 771
SacI GAGCTC 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 308
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 2 cut(s) 30, 160
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 2 cut(s) 674, 756
SduI GDGCHC 1 cut(s) 699
SfaNI GCATC 4 cut(s) 199, 421, 655, 730
SfcI CTRYAG 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 2 cut(s) 30, 160
SmiMI CAYNNNNRTG 2 cut(s) 422, 771
SmlI CTYRAG 2 cut(s) 173, 692
SmoI CTYRAG 2 cut(s) 173, 692
Sse9I AATT 5 cut(s) 389, 511, 546, 568, 638
SspMI CTAG 3 cut(s) 420, 435, 458
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 2 cut(s) 672, 754
TaaI ACNGT 1 cut(s) 657
TaiI ACGT 5 cut(s) 445, 490, 586, 610, 763
TaqI TCGA 2 cut(s) 39, 129
TasI AATT 5 cut(s) 389, 511, 546, 568, 638
TatI WGTACW 1 cut(s) 292
TauI GCSGC 3 cut(s) 11, 266, 326
TfiI GAWTC 2 cut(s) 108, 169
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 18
TseI GCWGC 7 cut(s) 81, 212, 272, 326, 329, 446, 503
TspDTI ATGAA 2 cut(s) 270, 618
TspGWI ACGGA 3 cut(s) 42, 133, 326
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 30, 160
XapI RAATTY 2 cut(s) 511, 638
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 3 cut(s) 420, 435, 458
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.