Rh2AG032400

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003445
Physical Location & Seq
Forward (+)
2236588 .. 2239375
2788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG032400.1

Sequence Viewer

Length: 831 bp
ATGTCTGCCGTAGTGTGCGGGAAGAGATCATCGATATTCGAAGAGCAGCTCCCCTCATCAACGTCATCGTCTCCTCCCGTTTCCTCCAAGAGAATTCGCTTTTCTTCTTCTTCTTCTTCTTCTCCGGTACGCTTCTCCCCTCCCACGACGACGTCGTTTGGTTCGTCCTTCATCGACCAGCTCAGGGCCGTTTTCCCGGACATGGATAATCAGCTTCTGGAGAGAGCACTTGAAGAATGCGGTAACGATTTGGATTCAGCTATCAGAAGTTTGAATGAGCTGCGCTTAAGTTCTGCTGCAAATAAATCCGGTGTAGCATCAGAAGCAAATGTGCAACCCCAGGCACAAGGTGCAGTAGCAACAAATGGAGAAGTTTCTGGTAATGATGATCTATCAGCACAAAATAACCTGCCCACGCAAGGAGCCGACTGGGTGGAGCTCTTTGTTAGAGAGATGATGAGTGCCTCCAACATGGATGATGCTAAAGCCCGTGCTTCAAGAGTGCTTGAGGTCTTGGAGAAATCCATCTGCTCACGTGCATCCACAGAGGCAGCCCAAAGCTTTCACCAGGAAAATTTGATGCTGAAGGAACAAGTGGAAGCTCTTATTCAGGAAAATACAATCCTGAAGCGAGCTGTATCCACTCAGCATGAACGTCAAAAAGAGTATGAAGATAGAGGCCAGGAATTGGAGCATCTGAAGCAAGTGGTGGCCCAGTACCAGGAACAACTGAGAACTCTTGAGGTGAACAACTATGCACTTACGATGCACCTGAAGCAAGCTCAGCAAAGCAGCTCCATTCCGGGGCGATTTCACCCTGATGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

276

Amino Acids

30.43

Weight (kDa)

5.17

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 58 - 94 8.1e-07 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 155
Acc36I ACCTGC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 688
AciI CCGC 2 cut(s) 18, 240
AcsI RAATTY 2 cut(s) 93, 574
AcuI CTGAAG 4 cut(s) 605, 647, 719, 794
AcvI CACGTG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 152
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 129, 719
AfiI CCNNNNNNNGG 6 cut(s) 184, 202, 419, 688, 721, 804
AflII CTTAAG 1 cut(s) 286
AgsI TTSAA 3 cut(s) 233, 274, 498
AjnI CCWGG 4 cut(s) 339, 567, 681, 720
Alw21I GWGCWC 2 cut(s) 229, 441
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 3 cut(s) 186, 679, 711
ApeKI GCWGC 5 cut(s) 46, 280, 296, 551, 792
ApoI RAATTY 2 cut(s) 93, 574
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 186, 712
AsuC2I CCSGG 2 cut(s) 197, 804
AsuHPI GGTGA 3 cut(s) 557, 757, 806
AsuII TTCGAA 1 cut(s) 39
BanII GRGCYC 1 cut(s) 441
BbrPI CACGTG 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 229, 441
BbvI GCAGC 5 cut(s) 58, 267, 283, 563, 804
BccI CCATC 1 cut(s) 533
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 4 cut(s) 341, 569, 683, 722
BciVI GTATCC 1 cut(s) 649
BcnI CCSGG 2 cut(s) 197, 804
BcoDI GTCTC 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 286
BfuAI ACCTGC 1 cut(s) 417
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 5 cut(s) 47, 281, 297, 552, 793
BlpI GCTNAGC 1 cut(s) 783
BlsI GCNGC 5 cut(s) 48, 282, 298, 553, 794
Bme1390I CCNGG 6 cut(s) 197, 341, 569, 683, 722, 804
BmgT120I GGNCC 2 cut(s) 186, 712
BmiI GGNNCC 1 cut(s) 424
BmrFI CCNGG 6 cut(s) 197, 341, 569, 683, 722, 804
BmrI ACTGGG 2 cut(s) 439, 709
BmsI GCATC 6 cut(s) 326, 469, 548, 570, 703, 756
BmuI ACTGGG 2 cut(s) 439, 709
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 182
Bpu1102I GCTNAGC 1 cut(s) 783
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 527, 761
BpuMI CCSGG 2 cut(s) 197, 804
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 339, 803
BsaWI WCCGGW 2 cut(s) 124, 308
Bsc4I CCNNNNNNNGG 6 cut(s) 184, 202, 419, 688, 721, 804
Bse1I ACTGG 2 cut(s) 434, 715
BseBI CCWGG 4 cut(s) 341, 569, 683, 722
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 339, 803
BseGI GGATG 2 cut(s) 481, 539
BseLI CCNNNNNNNGG 6 cut(s) 184, 202, 419, 688, 721, 804
BseMII CTCAG 4 cut(s) 196, 659, 722, 797
BseNI ACTGG 2 cut(s) 434, 715
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 5 cut(s) 58, 267, 283, 563, 804
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 3 cut(s) 188, 681, 713
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 229, 441
BsiSI CCGG 4 cut(s) 125, 197, 309, 803
BslI CCNNNNNNNGG 6 cut(s) 184, 202, 419, 688, 721, 804
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 3 cut(s) 188, 681, 713
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 229, 441
Bsp143I GATC 2 cut(s) 26, 388
Bsp1720I GCTNAGC 1 cut(s) 783
BspACI CCGC 2 cut(s) 18, 240
BspANI GGCC 3 cut(s) 188, 681, 713
BspCNI CTCAG 4 cut(s) 195, 658, 723, 796
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 424
BspMI ACCTGC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 286
BsrI ACTGG 2 cut(s) 434, 715
BssECI CCNNGG 2 cut(s) 339, 803
BssMI GATC 2 cut(s) 26, 388
BssNI GRCGYC 1 cut(s) 152
Bst2UI CCWGG 4 cut(s) 341, 569, 683, 722
Bst6I CTCTTC 2 cut(s) 17, 36
BstACI GRCGYC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBAI YACGTR 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 633, 780
BstDEI CTNAG 4 cut(s) 182, 645, 731, 783
BstF5I GGATG 2 cut(s) 481, 539
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 2 cut(s) 29, 391
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 26, 388
BstMWI GCNNNNNNNGC 5 cut(s) 323, 350, 700, 775, 784
BstNI CCWGG 4 cut(s) 341, 569, 683, 722
BstSCI CCNGG 6 cut(s) 195, 339, 567, 681, 720, 802
BstV1I GCAGC 5 cut(s) 58, 267, 283, 563, 804
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 3 cut(s) 188, 681, 713
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 481, 539
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 633, 780
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 2 cut(s) 186, 712
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 128, 718
CviAII CATG 3 cut(s) 202, 472, 650
CviQI GTAC 2 cut(s) 128, 718
DdeI CTNAG 4 cut(s) 182, 645, 731, 783
DpnI GATC 2 cut(s) 28, 390
DpnII GATC 2 cut(s) 26, 388
DraIII CACNNNGTG 1 cut(s) 350
Eam1104I CTCTTC 2 cut(s) 17, 36
EarI CTCTTC 2 cut(s) 17, 36
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 4 cut(s) 605, 647, 719, 794
Eco72I CACGTG 1 cut(s) 536
EcoICRI GAGCTC 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 4 cut(s) 339, 567, 681, 720
EcoT38I GRGCYC 1 cut(s) 441
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 3 cut(s) 205, 475, 653
FaiI YATR 5 cut(s) 203, 473, 651, 669, 756
FatI CATG 3 cut(s) 201, 471, 649
FauI CCCGC 1 cut(s) 11
Fnu4HI GCNGC 5 cut(s) 47, 281, 297, 552, 793
FokI GGATG 2 cut(s) 488, 526
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 5 cut(s) 47, 281, 297, 552, 793
GlaI GCGC 1 cut(s) 284
GluI GCNGC 5 cut(s) 47, 281, 297, 552, 793
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 3 cut(s) 188, 681, 713
HapII CCGG 4 cut(s) 125, 197, 309, 803
HhaI GCGC 1 cut(s) 285
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 3 cut(s) 205, 475, 653
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 1 cut(s) 254
HpaII CCGG 4 cut(s) 125, 197, 309, 803
HphI GGTGA 3 cut(s) 557, 757, 806
Hpy166II GTNNAC 1 cut(s) 748
Hpy188I TCNGA 3 cut(s) 266, 322, 699
Hpy188III TCNNGA 5 cut(s) 218, 498, 611, 625, 740
Hpy8I GTNNAC 1 cut(s) 748
Hpy99I CGWCG 3 cut(s) 151, 154, 157
HpyAV CCTTC 2 cut(s) 178, 580
HpyCH4IV ACGT 4 cut(s) 62, 152, 535, 655
HpyCH4V TGCA 6 cut(s) 299, 334, 353, 539, 758, 769
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 350, 700, 775, 784
HpyF3I CTNAG 4 cut(s) 182, 645, 731, 783
HpySE526I ACGT 4 cut(s) 62, 152, 535, 655
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 3 cut(s) 205, 475, 653
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 26, 388
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 5 cut(s) 54, 422, 436, 691, 800
Lsp1109I GCAGC 5 cut(s) 58, 267, 283, 563, 804
LweI GCATC 6 cut(s) 326, 469, 548, 570, 703, 756
MaeII ACGT 4 cut(s) 62, 152, 535, 655
MaeIII GTNAC 1 cut(s) 242
MalI GATC 2 cut(s) 28, 390
MboI GATC 2 cut(s) 26, 388
MhlI GDGCHC 2 cut(s) 229, 441
MluCI AATT 3 cut(s) 93, 574, 686
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 9 cut(s) 64, 84, 94, 150, 475, 502, 541, 671, 736
MseI TTAA 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 819
MspCI CTTAAG 1 cut(s) 286
MspI CCGG 4 cut(s) 125, 197, 309, 803
MspR9I CCNGG 6 cut(s) 197, 341, 569, 683, 722, 804
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 4 cut(s) 341, 569, 683, 722
MwoI GCNNNNNNNGC 5 cut(s) 323, 350, 700, 775, 784
NciI CCSGG 2 cut(s) 197, 804
NdeII GATC 2 cut(s) 26, 388
NlaIII CATG 3 cut(s) 205, 475, 653
NlaIV GGNNCC 1 cut(s) 424
NspV TTCGAA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 161
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 688
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 5 cut(s) 48, 282, 298, 553, 794
PmaCI CACGTG 1 cut(s) 536
PmlI CACGTG 1 cut(s) 536
Ppu21I YACGTR 1 cut(s) 536
Psp124BI GAGCTC 1 cut(s) 441
Psp6I CCWGG 4 cut(s) 339, 567, 681, 720
PspCI CACGTG 1 cut(s) 536
PspGI CCWGG 4 cut(s) 339, 567, 681, 720
PspN4I GGNNCC 1 cut(s) 424
PspPI GGNCC 2 cut(s) 186, 712
PstNI CAGNNNCTG 1 cut(s) 217
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 2 cut(s) 129, 719
RsaNI GTAC 2 cut(s) 128, 718
RseI CAYNNNNRTG 1 cut(s) 819
SacI GAGCTC 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 5 cut(s) 47, 281, 297, 552, 793
Sau3AI GATC 2 cut(s) 26, 388
Sau96I GGNCC 2 cut(s) 186, 712
ScrFI CCNGG 6 cut(s) 197, 341, 569, 683, 722, 804
SduI GDGCHC 2 cut(s) 229, 441
SfaNI GCATC 6 cut(s) 326, 469, 548, 570, 703, 756
SfuI TTCGAA 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 3 cut(s) 286, 506, 740
SmoI CTYRAG 3 cut(s) 286, 506, 740
Sse9I AATT 3 cut(s) 93, 574, 686
SsiI CCGC 2 cut(s) 18, 240
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 6 cut(s) 195, 339, 567, 681, 720, 802
TaiI ACGT 4 cut(s) 65, 155, 538, 658
TaqI TCGA 3 cut(s) 32, 39, 174
TasI AATT 3 cut(s) 93, 574, 686
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseI GCWGC 5 cut(s) 46, 280, 296, 551, 792
TspDTI ATGAA 3 cut(s) 160, 666, 684
Tth111I GACNNNGTC 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 688
Vha464I CTTAAG 1 cut(s) 286
XapI RAATTY 2 cut(s) 93, 574
ZraI GACGTC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.