MD02G1026900.v1.1

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003445
Physical Location & Seq
Reverse (-)
2049041 .. 2052049
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1026900.v1.1.491

Sequence Viewer

Length: 828 bp
ATGTCTGCCGTTGTGTGCGGGAAGAGATCATCGATTTTCGAAGACAACTCGCTGCCGTCGACTTCCCCTCCCGTCTCCTCCGCTTCCAAGAGAATCCGCTGCTCTTCTTCTTCCTCCCCTGTTCGCTTCTCTCCTCCCTCCACGACGTCGTTTGCTGCCTCGCATTTGATCGAACAACTCAGGGCCGTTTTCCCGGACATGGACAACCAGCTTCTGGAGGGAGCACTTGAAGAATGTGGTGGTGATTTGGATTCGGCTATCAAAAGTTTGAATGAGCTACGTTTAGGTTCTGCAGCAGGAAAATCTGATATAGCGCCAGAAGCAAATGTGCAACTCCAATCACAAAGTGCGGTAGCAACAAATGGAGAGGTTGCAGTAACTGATGATCCATCAGTGCAAAATAACCTTCCCAAGGATGGAGCTGAGTGGGTGGAGCTCGTTGTCAGAGAGATGATGAGTGCCTCTAACATGTATGATGCTAAAGCTCGTGCTTCAAGGGTGCTTGAGGCTTTGGAGAAGTCCATTAGTGCTTGTGCAACCACAGAGGTAGCCCAAAGCTTTCGCCAGGAAAATATGATGCTGAAGGAACAAATGGAAGCTATTATTCAGGAAAATGCAATTTTAAAGCGAGCTGTATCCACTCAGCATGAACGGCAGAAGGAGTTTGAAGATAGAGGCCAGGAATTGCAGCATCTGAAGCAACTGGTGGCCCAGTACCAGGAACAGTTGAGAACCCTCGAGGTGAACAACTACGCACTTACAATGCACCTAAAGCAGGCACAACAAAGCAGCTCCATCCCAGGGCGCTTTCATCCGGATGTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

276

Amino Acids

30.16

Weight (kDa)

5.17

Isoelectric Point (pI)

64.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 56 - 93 4.3e-07 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 149
AbsI CCTCGAGG 1 cut(s) 737
AccB7I CCANNNNNTGG 1 cut(s) 214
AccI GTMKAC 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 814
AciI CCGC 4 cut(s) 18, 81, 97, 350
AclWI GGATC 1 cut(s) 380
AcuI CTGAAG 2 cut(s) 602, 716
AcyI GRCGYC 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 7 cut(s) 199, 214, 412, 416, 718, 775, 801
AflIII ACRYGT 1 cut(s) 468
AgsI TTSAA 4 cut(s) 230, 271, 495, 668
AjnI CCWGG 4 cut(s) 564, 678, 717, 799
AluBI AGCT 9 cut(s) 211, 277, 422, 436, 485, 558, 599, 632, 792
AluI AGCT 9 cut(s) 211, 277, 422, 436, 485, 558, 599, 632, 792
Alw21I GWGCWC 2 cut(s) 226, 438
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 1 cut(s) 380
AlwNI CAGNNNCTG 3 cut(s) 214, 380, 694
Ama87I CYCGRG 1 cut(s) 737
Aor13HI TCCGGA 1 cut(s) 814
AoxI GGCC 3 cut(s) 183, 676, 708
ApeKI GCWGC 6 cut(s) 52, 99, 155, 293, 688, 789
AspLEI GCGC 2 cut(s) 316, 807
AspS9I GGNCC 2 cut(s) 183, 709
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 254, 754
AsuII TTCGAA 1 cut(s) 39
AvaI CYCGRG 1 cut(s) 737
BanII GRGCYC 1 cut(s) 438
BauI CACGAG 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 226, 438
BbvI GCAGC 6 cut(s) 39, 86, 142, 305, 700, 801
BccI CCATC 3 cut(s) 397, 410, 803
BceAI ACGGC 3 cut(s) 40, 170, 668
BciT130I CCWGG 4 cut(s) 566, 680, 719, 801
BciVI GTATCC 1 cut(s) 646
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 79
BfmI CTRYAG 1 cut(s) 291
BfoI RGCGCY 2 cut(s) 317, 808
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 6 cut(s) 53, 100, 156, 294, 689, 790
BlsI GCNGC 6 cut(s) 54, 101, 157, 295, 690, 791
Bme1390I CCNGG 5 cut(s) 194, 566, 680, 719, 801
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 2 cut(s) 183, 709
BmrFI CCNGG 5 cut(s) 194, 566, 680, 719, 801
BmrI ACTGGG 1 cut(s) 706
BmsI GCATC 3 cut(s) 466, 567, 700
BmuI ACTGGG 1 cut(s) 706
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 411, 799, 800
BsaWI WCCGGW 1 cut(s) 814
BsaXI ACNNNNNCTCC 2 cut(s) 52, 82
Bsc4I CCNNNNNNNGG 7 cut(s) 199, 214, 412, 416, 718, 775, 801
Bse1I ACTGG 2 cut(s) 708, 712
BseAI TCCGGA 1 cut(s) 814
BseBI CCWGG 4 cut(s) 566, 680, 719, 801
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 411, 799, 800
BseGI GGATG 4 cut(s) 421, 795, 811, 823
BseLI CCNNNNNNNGG 7 cut(s) 199, 214, 412, 416, 718, 775, 801
BseMII CTCAG 3 cut(s) 193, 414, 656
BseNI ACTGG 2 cut(s) 708, 712
BseRI GAGGAG 2 cut(s) 67, 123
BseXI GCAGC 6 cut(s) 39, 86, 142, 305, 700, 801
BshFI GGCC 3 cut(s) 185, 678, 710
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 226, 438
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 2 cut(s) 194, 815
BslI CCNNNNNNNGG 7 cut(s) 199, 214, 412, 416, 718, 775, 801
BsmAI GTCTC 1 cut(s) 79
BsmBI CGTCTC 1 cut(s) 79
BsnI GGCC 3 cut(s) 185, 678, 710
BsoBI CYCGRG 1 cut(s) 737
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 226, 438
Bsp13I TCCGGA 1 cut(s) 814
Bsp143I GATC 3 cut(s) 26, 168, 385
BspACI CCGC 4 cut(s) 18, 81, 97, 350
BspANI GGCC 3 cut(s) 185, 678, 710
BspCNI CTCAG 3 cut(s) 192, 415, 655
BspDI ATCGAT 1 cut(s) 32
BspEI TCCGGA 1 cut(s) 814
BspMAI CTGCAG 1 cut(s) 295
BspPI GGATC 1 cut(s) 380
BspQI GCTCTTC 1 cut(s) 109
BspT104I TTCGAA 1 cut(s) 39
BsrI ACTGG 2 cut(s) 708, 712
BssECI CCNNGG 3 cut(s) 411, 799, 800
BssMI GATC 3 cut(s) 26, 168, 385
BssNI GRCGYC 1 cut(s) 146
BssSI CACGAG 1 cut(s) 486
BssT1I CCWWGG 1 cut(s) 411
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 4 cut(s) 566, 680, 719, 801
Bst4CI ACNGT 1 cut(s) 726
Bst6I CTCTTC 2 cut(s) 17, 109
BstACI GRCGYC 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 630, 777
BstDEI CTNAG 3 cut(s) 179, 423, 642
BstENI CCTNNNNNAGG 2 cut(s) 410, 773
BstF5I GGATG 4 cut(s) 421, 795, 811, 823
BstH2I RGCGCY 2 cut(s) 317, 808
BstHHI GCGC 2 cut(s) 316, 807
BstKTI GATC 3 cut(s) 29, 171, 388
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 3 cut(s) 26, 168, 385
BstMWI GCNNNNNNNGC 4 cut(s) 320, 652, 697, 772
BstNI CCWGG 4 cut(s) 566, 680, 719, 801
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 5 cut(s) 192, 564, 678, 717, 799
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 6 cut(s) 39, 86, 142, 305, 700, 801
BstV2I GAAGAC 1 cut(s) 48
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 3 cut(s) 185, 678, 710
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 4 cut(s) 421, 795, 811, 823
BtsIMutI CAGTG 1 cut(s) 399
Cac8I GCNNGC 2 cut(s) 630, 777
CaiI CAGNNNCTG 3 cut(s) 214, 380, 694
CfoI GCGC 2 cut(s) 316, 807
Cfr13I GGNCC 2 cut(s) 183, 709
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 1 cut(s) 715
CviAII CATG 3 cut(s) 199, 469, 647
CviQI GTAC 1 cut(s) 715
DdeI CTNAG 3 cut(s) 179, 423, 642
DpnI GATC 3 cut(s) 28, 170, 387
DpnII GATC 3 cut(s) 26, 168, 385
DraI TTTAAA 1 cut(s) 624
DraIII CACNNNGTG 1 cut(s) 347
Eam1104I CTCTTC 2 cut(s) 17, 109
EarI CTCTTC 2 cut(s) 17, 109
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 411
Eco24I GRGCYC 1 cut(s) 438
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 602, 716
Eco88I CYCGRG 1 cut(s) 737
EcoICRI GAGCTC 1 cut(s) 436
EcoNI CCTNNNNNAGG 2 cut(s) 410, 773
EcoRII CCWGG 4 cut(s) 564, 678, 717, 799
EcoT14I CCWWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 411
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 3 cut(s) 202, 472, 650
FaiI YATR 6 cut(s) 200, 311, 470, 474, 575, 648
FatI CATG 3 cut(s) 198, 468, 646
FauI CCCGC 1 cut(s) 11
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 6 cut(s) 53, 100, 156, 294, 689, 790
FokI GGATG 3 cut(s) 428, 782, 798
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 6 cut(s) 53, 100, 156, 294, 689, 790
GlaI GCGC 2 cut(s) 315, 806
GluI GCNGC 6 cut(s) 53, 100, 156, 294, 689, 790
GsuI CTGGAG 1 cut(s) 236
HaeII RGCGCY 2 cut(s) 317, 808
HaeIII GGCC 3 cut(s) 185, 678, 710
HapII CCGG 2 cut(s) 194, 815
HhaI GCGC 2 cut(s) 316, 807
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 3 cut(s) 202, 472, 650
Hin6I GCGC 2 cut(s) 314, 805
HinP1I GCGC 2 cut(s) 314, 805
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HindIII AAGCTT 1 cut(s) 556
HinfI GANTC 2 cut(s) 93, 251
HpaII CCGG 2 cut(s) 194, 815
HphI GGTGA 2 cut(s) 254, 754
Hpy166II GTNNAC 2 cut(s) 60, 745
Hpy188I TCNGA 3 cut(s) 307, 446, 696
Hpy188III TCNNGA 3 cut(s) 215, 608, 815
Hpy8I GTNNAC 2 cut(s) 60, 745
Hpy99I CGWCG 3 cut(s) 61, 148, 151
HpyAV CCTTC 3 cut(s) 416, 577, 652
HpyCH4III ACNGT 1 cut(s) 726
HpyCH4IV ACGT 2 cut(s) 146, 280
HpyCH4V TGCA 8 cut(s) 293, 331, 374, 397, 536, 617, 688, 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 652, 697, 772
HpyF3I CTNAG 3 cut(s) 179, 423, 642
HpySE526I ACGT 2 cut(s) 146, 280
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 3 cut(s) 202, 472, 650
HspAI GCGC 2 cut(s) 314, 805
Kpn2I TCCGGA 1 cut(s) 814
Kzo9I GATC 3 cut(s) 26, 168, 385
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 4 cut(s) 221, 419, 433, 797
Lsp1109I GCAGC 6 cut(s) 39, 86, 142, 305, 700, 801
LweI GCATC 3 cut(s) 466, 567, 700
MaeII ACGT 2 cut(s) 146, 280
MaeIII GTNAC 1 cut(s) 376
MalI GATC 3 cut(s) 28, 170, 387
MboI GATC 3 cut(s) 26, 168, 385
MboII GAAGA 7 cut(s) 34, 53, 96, 99, 102, 242, 680
MhlI GDGCHC 2 cut(s) 226, 438
MluCI AATT 2 cut(s) 618, 683
MroI TCCGGA 1 cut(s) 814
MseI TTAA 1 cut(s) 623
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 2 cut(s) 194, 815
MspR9I CCNGG 5 cut(s) 194, 566, 680, 719, 801
MvaI CCWGG 4 cut(s) 566, 680, 719, 801
MwoI GCNNNNNNNGC 4 cut(s) 320, 652, 697, 772
NciI CCSGG 1 cut(s) 194
NdeII GATC 3 cut(s) 26, 168, 385
NlaIII CATG 3 cut(s) 202, 472, 650
NspI RCATGY 1 cut(s) 472
NspV TTCGAA 1 cut(s) 39
PaeR7I CTCGAG 1 cut(s) 737
PasI CCCWGGG 1 cut(s) 800
PciI ACATGT 1 cut(s) 468
PciSI GCTCTTC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PfeI GAWTC 2 cut(s) 93, 251
PflMI CCANNNNNTGG 1 cut(s) 214
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 6 cut(s) 54, 101, 157, 295, 690, 791
PscI ACATGT 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 4 cut(s) 564, 678, 717, 799
PspGI CCWGG 4 cut(s) 564, 678, 717, 799
PspPI GGNCC 2 cut(s) 183, 709
PspXI VCTCGAGB 1 cut(s) 737
PstI CTGCAG 1 cut(s) 295
PstNI CAGNNNCTG 3 cut(s) 214, 380, 694
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
RseI CAYNNNNRTG 1 cut(s) 816
SacI GAGCTC 1 cut(s) 438
SalI GTCGAC 1 cut(s) 58
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 1 cut(s) 623
SatI GCNGC 6 cut(s) 53, 100, 156, 294, 689, 790
Sau3AI GATC 3 cut(s) 26, 168, 385
Sau96I GGNCC 2 cut(s) 183, 709
ScrFI CCNGG 5 cut(s) 194, 566, 680, 719, 801
SduI GDGCHC 2 cut(s) 226, 438
SfaNI GCATC 3 cut(s) 466, 567, 700
SfcI CTRYAG 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 737
SfuI TTCGAA 1 cut(s) 39
SlaI CTCGAG 1 cut(s) 737
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 2 cut(s) 503, 737
SmoI CTYRAG 2 cut(s) 503, 737
Sse9I AATT 2 cut(s) 618, 683
SsiI CCGC 4 cut(s) 18, 81, 97, 350
SstI GAGCTC 1 cut(s) 438
StyD4I CCNGG 5 cut(s) 192, 564, 678, 717, 799
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 1 cut(s) 726
TaiI ACGT 2 cut(s) 149, 283
TaqI TCGA 5 cut(s) 32, 39, 59, 171, 738
TasI AATT 2 cut(s) 618, 683
TfiI GAWTC 2 cut(s) 93, 251
Tru1I TTAA 1 cut(s) 623
Tru9I TTAA 1 cut(s) 623
TscAI CASTG 1 cut(s) 399
TseI GCWGC 6 cut(s) 52, 99, 155, 293, 688, 789
TspDTI ATGAA 2 cut(s) 663, 800
TspRI CASTG 1 cut(s) 399
Van91I CCANNNNNTGG 1 cut(s) 214
XagI CCTNNNNNAGG 2 cut(s) 410, 773
XceI RCATGY 1 cut(s) 472
XhoI CTCGAG 1 cut(s) 737
XmiI GTMKAC 1 cut(s) 59
ZraI GACGTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.