Rroxscaffold_2G00109500

CUE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003445
Physical Location & Seq
Forward (+)
33669450 .. 33672896
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109500.1

Sequence Viewer

Length: 807 bp
ATGTCTGCGGCAGTGTGCGGAAGCAAGAGGTCCTTCTTCGAAGAGCTTCCTCCGTCGCCTCCGTTAACCAAGAGACTCCGCTGCTCTTCTTCTACCTCCCCCGTTCGATTCTCTTCTCCTTATCTCATTGATCAGCTCCGTTCCGTCTTCCCTCACATGGACCCTCAGATTCTTGAGCGAGCATTGGAAGAGTGTGGCAATGATATTGATGCTGCTATCAAGAGACTGCATGAGCTTCACATAGGATATGGTGATGAAAATGCCGTTCCTGCTGCAGAACCGAATGATATTTTACAGAAAGCTGCAGCTGCTTCTGAGAACCCATCAGCTCCAAGCAACTTGCCTGCAGATGGTGCGGAATGGGTTGAATTGTTTGTCAGGGAAATGATGAGTGCCTCTAGTGTGGATGATGCTAGGGGACGTGCTGCAAAAGTGCTAGAGGTTTTAGAGAAATCCATCAGTTCACGTGCTGGTGCGGAGGCAGCCCAAAATTTTCAGAAGGAGAATATGCTACTGAAAGAACAAATCGAAGGACTGATAAGGGACAATTCCGTCCTCAAACGTGCTGTGTCTATCCAGCATGAACGTCAGAAAGAGTATGAGGGCAGAAGCCAAGAATTCCAGCATCTGAAGCAGTTAGTAACTCAATATCAGGAGCAGTTGAGGACACTTGAGGGTATATTTTCCTCAGTTAGAGGGATGGACATGTCATTAGACTGGGTTGATGAGGCTCAACAACTATGCCTTGACAATGCATCTGAAGCAGGCACAGCAAGGCAACTCCATGCCTGGACGTTTCCATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

268

Amino Acids

29.83

Weight (kDa)

5.12

Isoelectric Point (pI)

58.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 43 - 76 1.7e-08 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 551
AciI CCGC 5 cut(s) 8, 18, 79, 356, 476
AcsI RAATTY 2 cut(s) 490, 617
AcuI CTGAAG 2 cut(s) 650, 780
AcvI CACGTG 1 cut(s) 467
AfiI CCNNNNNNNGG 2 cut(s) 157, 350
AflIII ACRYGT 1 cut(s) 705
AgsI TTSAA 1 cut(s) 368
AjiI CACGTC 1 cut(s) 422
AjnI CCWGG 1 cut(s) 788
AluBI AGCT 6 cut(s) 46, 136, 235, 302, 308, 329
AluI AGCT 6 cut(s) 46, 136, 235, 302, 308, 329
Alw26I GTCTC 2 cut(s) 67, 217
AlwNI CAGNNNCTG 1 cut(s) 628
ApeKI GCWGC 8 cut(s) 81, 212, 272, 302, 305, 308, 425, 482
ApoI RAATTY 2 cut(s) 490, 617
Asp700I GAANNNNTTC 1 cut(s) 45
AspS9I GGNCC 2 cut(s) 30, 160
AsuHPI GGTGA 1 cut(s) 263
AsuII TTCGAA 1 cut(s) 39
AvaII GGWCC 2 cut(s) 30, 160
BbrPI CACGTG 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 139
BbvI GCAGC 8 cut(s) 68, 199, 259, 289, 295, 317, 412, 494
BccI CCATC 4 cut(s) 331, 344, 464, 694
BceAI ACGGC 1 cut(s) 248
BciT130I CCWGG 1 cut(s) 790
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 67, 217
BfaI CTAG 3 cut(s) 399, 414, 437
BfmI CTRYAG 3 cut(s) 273, 303, 345
BisI GCNGC 9 cut(s) 9, 82, 213, 273, 303, 306, 309, 426, 483
BlsI GCNGC 9 cut(s) 10, 83, 214, 274, 304, 307, 310, 427, 484
Bme1390I CCNGG 1 cut(s) 790
Bme18I GGWCC 2 cut(s) 30, 160
BmgBI CACGTC 1 cut(s) 422
BmgT120I GGNCC 2 cut(s) 30, 160
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 790
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 4 cut(s) 199, 400, 634, 764
BmuI ACTGGG 1 cut(s) 727
BpiI GAAGAC 1 cut(s) 139
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 194, 692
BsaAI YACGTR 1 cut(s) 467
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 350
Bse1I ACTGG 1 cut(s) 722
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 1 cut(s) 790
BseGI GGATG 3 cut(s) 412, 705, 800
BseLI CCNNNNNNNGG 2 cut(s) 157, 350
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 3 cut(s) 179, 306, 702
BseNI ACTGG 1 cut(s) 722
BseXI GCAGC 8 cut(s) 68, 199, 259, 289, 295, 317, 412, 494
BslFI GGGAC 2 cut(s) 432, 557
BslI CCNNNNNNNGG 2 cut(s) 157, 350
BsmAI GTCTC 2 cut(s) 67, 217
BsmFI GGGAC 2 cut(s) 432, 557
Bsp119I TTCGAA 1 cut(s) 39
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 5 cut(s) 8, 18, 79, 356, 476
BspCNI CTCAG 3 cut(s) 178, 307, 701
BspLI GGNNCC 1 cut(s) 162
BspMAI CTGCAG 3 cut(s) 277, 307, 349
BspQI GCTCTTC 2 cut(s) 36, 91
BspT104I TTCGAA 1 cut(s) 39
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 1 cut(s) 722
BssMI GATC 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 790
Bst6I CTCTTC 4 cut(s) 36, 91, 118, 183
BstAPI GCANNNNNTGC 1 cut(s) 353
BstBAI YACGTR 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 3 cut(s) 180, 345, 766
BstDEI CTNAG 3 cut(s) 165, 315, 688
BstF5I GGATG 3 cut(s) 412, 705, 800
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 2 cut(s) 67, 217
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 7 cut(s) 269, 308, 353, 482, 631, 761, 770
BstNI CCWGG 1 cut(s) 790
BstNSI RCATGY 1 cut(s) 709
BstSCI CCNGG 1 cut(s) 788
BstSFI CTRYAG 3 cut(s) 273, 303, 345
BstV1I GCAGC 8 cut(s) 68, 199, 259, 289, 295, 317, 412, 494
BstV2I GAAGAC 1 cut(s) 139
BtrI CACGTC 1 cut(s) 422
BtsCI GGATG 3 cut(s) 412, 705, 800
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 3 cut(s) 180, 345, 766
CaiI CAGNNNCTG 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 30, 160
CviAII CATG 5 cut(s) 157, 230, 581, 706, 785
CviJI RGCY 9 cut(s) 46, 136, 235, 302, 308, 329, 485, 612, 731
CviKI_1 RGCY 9 cut(s) 46, 136, 235, 302, 308, 329, 485, 612, 731
DdeI CTNAG 3 cut(s) 165, 315, 688
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
DrdI GACNNNNNNGTC 1 cut(s) 551
DseDI GACNNNNNNGTC 1 cut(s) 551
Eam1104I CTCTTC 4 cut(s) 36, 91, 118, 183
EarI CTCTTC 4 cut(s) 36, 91, 118, 183
Eco47I GGWCC 2 cut(s) 30, 160
Eco57I CTGAAG 2 cut(s) 650, 780
Eco72I CACGTG 1 cut(s) 467
EcoO109I RGGNCCY 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 788
EcoT22I ATGCAT 1 cut(s) 757
FaeI CATG 5 cut(s) 160, 233, 584, 709, 788
FalI AAGNNNNNCTT 2 cut(s) 17, 49
FaqI GGGAC 2 cut(s) 432, 557
FatI CATG 5 cut(s) 156, 229, 580, 705, 784
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 9 cut(s) 9, 82, 213, 273, 303, 306, 309, 426, 483
FokI GGATG 3 cut(s) 419, 712, 787
Fsp4HI GCNGC 9 cut(s) 9, 82, 213, 273, 303, 306, 309, 426, 483
FspBI CTAG 3 cut(s) 399, 414, 437
GluI GCNGC 9 cut(s) 9, 82, 213, 273, 303, 306, 309, 426, 483
Hin1II CATG 5 cut(s) 160, 233, 584, 709, 788
HincII GTYRAC 1 cut(s) 66
HindII GTYRAC 1 cut(s) 66
HinfI GANTC 3 cut(s) 75, 108, 169
HpaI GTTAAC 1 cut(s) 66
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 2 cut(s) 66, 464
Hpy188I TCNGA 6 cut(s) 168, 316, 498, 591, 630, 760
Hpy188III TCNNGA 4 cut(s) 173, 220, 653, 804
Hpy8I GTNNAC 2 cut(s) 66, 464
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 43, 493, 524
HpyCH4IV ACGT 5 cut(s) 421, 466, 562, 586, 794
HpyCH4V TGCA 6 cut(s) 229, 275, 305, 347, 428, 755
HpyF10VI GCNNNNNNNGC 7 cut(s) 269, 308, 353, 482, 631, 761, 770
HpyF3I CTNAG 3 cut(s) 165, 315, 688
HpySE526I ACGT 5 cut(s) 421, 466, 562, 586, 794
Hsp92II CATG 5 cut(s) 160, 233, 584, 709, 788
Ksp22I TGATCA 1 cut(s) 130
KspAI GTTAAC 1 cut(s) 66
Kzo9I GATC 1 cut(s) 130
LguI GCTCTTC 2 cut(s) 36, 91
LmnI GCTCC 3 cut(s) 141, 334, 655
Lsp1109I GCAGC 8 cut(s) 68, 199, 259, 289, 295, 317, 412, 494
LweI GCATC 4 cut(s) 199, 400, 634, 764
MaeI CTAG 3 cut(s) 399, 414, 437
MaeII ACGT 5 cut(s) 421, 466, 562, 586, 794
MaeIII GTNAC 1 cut(s) 640
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 7 cut(s) 28, 53, 78, 81, 105, 139, 200
MluCI AATT 4 cut(s) 368, 490, 547, 617
MlyI GAGTC 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 757
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 1 cut(s) 65
MspA1I CMGCKG 2 cut(s) 81, 308
MspR9I CCNGG 1 cut(s) 790
MvaI CCWGG 1 cut(s) 790
MwoI GCNNNNNNNGC 7 cut(s) 269, 308, 353, 482, 631, 761, 770
NdeII GATC 1 cut(s) 130
NlaIII CATG 5 cut(s) 160, 233, 584, 709, 788
NlaIV GGNNCC 1 cut(s) 162
NsiI ATGCAT 1 cut(s) 757
NspI RCATGY 1 cut(s) 709
NspV TTCGAA 1 cut(s) 39
PciI ACATGT 1 cut(s) 705
PciSI GCTCTTC 2 cut(s) 36, 91
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 108, 169
PkrI GCNGC 9 cut(s) 10, 83, 214, 274, 304, 307, 310, 427, 484
PleI GAGTC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 467
PmlI CACGTG 1 cut(s) 467
PpsI GAGTC 1 cut(s) 69
Ppu21I YACGTR 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 30
PscI ACATGT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 788
PspCI CACGTG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 788
PspN4I GGNNCC 1 cut(s) 162
PspPI GGNCC 2 cut(s) 30, 160
PspPPI RGGWCCY 1 cut(s) 30
PstI CTGCAG 3 cut(s) 277, 307, 349
PstNI CAGNNNCTG 1 cut(s) 628
PvuII CAGCTG 1 cut(s) 308
SapI GCTCTTC 2 cut(s) 36, 91
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 9 cut(s) 9, 82, 213, 273, 303, 306, 309, 426, 483
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 2 cut(s) 30, 160
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 790
SfaNI GCATC 4 cut(s) 199, 400, 634, 764
SfcI CTRYAG 3 cut(s) 273, 303, 345
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 2 cut(s) 30, 160
SmlI CTYRAG 2 cut(s) 173, 671
SmoI CTYRAG 2 cut(s) 173, 671
Sse9I AATT 4 cut(s) 368, 490, 547, 617
SsiI CCGC 5 cut(s) 8, 18, 79, 356, 476
SspMI CTAG 3 cut(s) 399, 414, 437
StyD4I CCNGG 1 cut(s) 788
TaiI ACGT 5 cut(s) 424, 469, 565, 589, 797
TaqI TCGA 3 cut(s) 39, 106, 528
TasI AATT 4 cut(s) 368, 490, 547, 617
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 108, 169
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TscAI CASTG 1 cut(s) 18
TseI GCWGC 8 cut(s) 81, 212, 272, 302, 305, 308, 425, 482
TspDTI ATGAA 2 cut(s) 270, 597
TspGWI ACGGA 5 cut(s) 42, 51, 128, 133, 541
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 30, 160
XapI RAATTY 2 cut(s) 490, 617
XceI RCATGY 1 cut(s) 709
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 3 cut(s) 399, 414, 437
Zsp2I ATGCAT 1 cut(s) 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.