Rh2CG031900

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003445
Physical Location & Seq
Forward (+)
2411147 .. 2413826
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG031900.1

Sequence Viewer

Length: 861 bp
ATGTCTGCCGTAGTGTGCGGGAAGAGATCATCGATATTCGAAGAGCAGCTCCCCTCATCAACGTCATCGTCTCCTCCCGTTTCCTCCAAGAGAATTCGCTTTTCTTCTTCTTCTTCTCCAGTACGCTTCTCCCCTCCCACGACGACGTCGTTTGGTTCGTCCTTCATCGACCAGCTCAGGGCCCTTTTCCCGGACATGGATAACCAGCTTCTGGAGAGAGCACTTGAAGAATGCGGTAATGATTTGGATTCAGCTATCAGAAGTTTGAATGAGCTGCGCTTAAGTTCTGCTGCAAATAAATCCGGTGTAGCATCAGAAGCAAATGTGCAACCCCAGGCACAAGGTGCAGTAGCAACAAATGGAGAAGTTTCTGGTAATGATGATCGATCAGGACAAAATAACCTGCCCACGCAAGGAGCCGATTGGGTGGAGCTCTTTGTTAGAGAGATGATGAGTGCCTCCAACATGGATGATGCTAAAGCCCGTGCTTCAAGAGTGCTTGAGGTCTTGGAGAAATCCATCTGCTCACGTGCATCAACAGAGGCAGCCCAAAGCTTTCACCAGGAAAATTTGATGCTGAAGGAACAAGTGGAAGCTCTTATTCAGGAAAATACAATCCTGAAGCGAGCTGTATCCACTCAGCATGAACGTCAAAAAGAGTATGAAGATAGAGGCCAGGAATTGGAGCATCTGAAGCAAGTGGTGGCCCAGTACCAGGAACAACTGAGAACTCTTGAGGTTGAGCTTTATACATTGTTATATATGGATGGAAGTTTGGGCTTTCTCTCTGTTTCAATTACAGGAATGTTATTATCTCACTTTTCCTCCCAGGGGGAAAAGAACATTTCCTCTGTTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

286

Amino Acids

31.45

Weight (kDa)

4.97

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 56 - 92 4.2e-07 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 859
AatII GACGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 411
AccB7I CCANNNNNTGG 2 cut(s) 211, 682
AciI CCGC 2 cut(s) 18, 234
AcsI RAATTY 2 cut(s) 93, 568
AcuI CTGAAG 3 cut(s) 599, 641, 713
AcvI CACGTG 1 cut(s) 530
AcyI GRCGYC 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 344
AfaI GTAC 2 cut(s) 123, 713
AfiI CCNNNNNNNGG 8 cut(s) 178, 190, 196, 211, 413, 682, 715, 831
AflII CTTAAG 1 cut(s) 280
AgsI TTSAA 4 cut(s) 227, 268, 492, 795
AjnI CCWGG 5 cut(s) 333, 561, 675, 714, 828
Alw21I GWGCWC 2 cut(s) 223, 435
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 211
AoxI GGCC 3 cut(s) 180, 673, 705
ApaI GGGCCC 1 cut(s) 184
ApeKI GCWGC 4 cut(s) 46, 274, 290, 545
ApoI RAATTY 2 cut(s) 93, 568
AspLEI GCGC 1 cut(s) 279
AspS9I GGNCC 3 cut(s) 180, 181, 706
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 551
AsuII TTCGAA 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 184
BanII GRGCYC 2 cut(s) 184, 435
BbrPI CACGTG 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 223, 435
BbvI GCAGC 4 cut(s) 58, 261, 277, 557
BccI CCATC 2 cut(s) 527, 761
BciT130I CCWGG 5 cut(s) 335, 563, 677, 716, 830
BciVI GTATCC 1 cut(s) 643
BcnI CCSGG 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 280
BfuAI ACCTGC 1 cut(s) 411
BfuI GTATCC 1 cut(s) 643
BisI GCNGC 4 cut(s) 47, 275, 291, 546
BlsI GCNGC 4 cut(s) 48, 276, 292, 547
Bme1390I CCNGG 6 cut(s) 191, 335, 563, 677, 716, 830
BmgT120I GGNCC 3 cut(s) 180, 181, 706
BmiI GGNNCC 2 cut(s) 182, 418
BmrFI CCNGG 6 cut(s) 191, 335, 563, 677, 716, 830
BmrI ACTGGG 1 cut(s) 703
BmsI GCATC 5 cut(s) 320, 463, 542, 564, 697
BmuI ACTGGG 1 cut(s) 703
BpmI CTGGAG 2 cut(s) 102, 233
Bpu10I CCTNAGC 1 cut(s) 176
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 521, 755
BpuMI CCSGG 1 cut(s) 191
Bsa29I ATCGAT 2 cut(s) 32, 385
BsaAI YACGTR 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 333, 828, 829
BsaWI WCCGGW 1 cut(s) 302
BsaXI ACNNNNNCTCC 2 cut(s) 809, 839
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 190, 196, 211, 413, 682, 715, 831
Bse1I ACTGG 2 cut(s) 119, 709
BseBI CCWGG 5 cut(s) 335, 563, 677, 716, 830
BseCI ATCGAT 2 cut(s) 32, 385
BseDI CCNNGG 3 cut(s) 333, 828, 829
BseGI GGATG 2 cut(s) 475, 772
BseLI CCNNNNNNNGG 8 cut(s) 178, 190, 196, 211, 413, 682, 715, 831
BseMII CTCAG 3 cut(s) 190, 653, 716
BseNI ACTGG 2 cut(s) 119, 709
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 4 cut(s) 58, 261, 277, 557
BsgI GTGCAG 1 cut(s) 366
BshFI GGCC 3 cut(s) 182, 675, 707
BshVI ATCGAT 2 cut(s) 32, 385
BsiHKAI GWGCWC 2 cut(s) 223, 435
BsiSI CCGG 2 cut(s) 191, 303
BslI CCNNNNNNNGG 8 cut(s) 178, 190, 196, 211, 413, 682, 715, 831
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 3 cut(s) 182, 675, 707
Bsp119I TTCGAA 1 cut(s) 39
Bsp120I GGGCCC 1 cut(s) 180
Bsp1286I GDGCHC 3 cut(s) 184, 223, 435
Bsp143I GATC 3 cut(s) 26, 382, 386
BspACI CCGC 2 cut(s) 18, 234
BspANI GGCC 3 cut(s) 182, 675, 707
BspCNI CTCAG 3 cut(s) 189, 652, 717
BspDI ATCGAT 2 cut(s) 32, 385
BspLI GGNNCC 2 cut(s) 182, 418
BspMI ACCTGC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 280
BsrI ACTGG 2 cut(s) 119, 709
BssECI CCNNGG 3 cut(s) 333, 828, 829
BssMI GATC 3 cut(s) 26, 382, 386
BssNI GRCGYC 1 cut(s) 146
Bst2UI CCWGG 5 cut(s) 335, 563, 677, 716, 830
Bst6I CTCTTC 2 cut(s) 17, 36
BstACI GRCGYC 1 cut(s) 146
BstAFI CTTAAG 1 cut(s) 280
BstAPI GCANNNNNTGC 1 cut(s) 344
BstBAI YACGTR 1 cut(s) 530
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 627
BstDEI CTNAG 3 cut(s) 176, 639, 725
BstF5I GGATG 2 cut(s) 475, 772
BstHHI GCGC 1 cut(s) 279
BstKTI GATC 3 cut(s) 29, 385, 389
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 3 cut(s) 26, 382, 386
BstMWI GCNNNNNNNGC 3 cut(s) 317, 344, 694
BstNI CCWGG 5 cut(s) 335, 563, 677, 716, 830
BstSCI CCNGG 6 cut(s) 189, 333, 561, 675, 714, 828
BstSLI GKGCMC 1 cut(s) 184
BstV1I GCAGC 4 cut(s) 58, 261, 277, 557
Bsu15I ATCGAT 2 cut(s) 32, 385
BsuI GTATCC 1 cut(s) 643
BsuRI GGCC 3 cut(s) 182, 675, 707
BsuTUI ATCGAT 2 cut(s) 32, 385
BtsCI GGATG 2 cut(s) 475, 772
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 1 cut(s) 627
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 180, 181, 706
ClaI ATCGAT 2 cut(s) 32, 385
Csp6I GTAC 2 cut(s) 122, 712
CviAII CATG 3 cut(s) 196, 466, 644
CviQI GTAC 2 cut(s) 122, 712
DdeI CTNAG 3 cut(s) 176, 639, 725
DpnI GATC 3 cut(s) 28, 384, 388
DpnII GATC 3 cut(s) 26, 382, 386
DraIII CACNNNGTG 1 cut(s) 344
Eam1104I CTCTTC 2 cut(s) 17, 36
EarI CTCTTC 2 cut(s) 17, 36
Ecl136II GAGCTC 1 cut(s) 433
Eco24I GRGCYC 2 cut(s) 184, 435
Eco53kI GAGCTC 1 cut(s) 433
Eco57I CTGAAG 3 cut(s) 599, 641, 713
Eco72I CACGTG 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 433
EcoO109I RGGNCCY 2 cut(s) 180, 181
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 5 cut(s) 333, 561, 675, 714, 828
EcoT38I GRGCYC 2 cut(s) 184, 435
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 3 cut(s) 199, 469, 647
FaiI YATR 9 cut(s) 197, 467, 645, 663, 750, 760, 762, 764, 859
FatI CATG 3 cut(s) 195, 465, 643
FauI CCCGC 1 cut(s) 11
Fnu4HI GCNGC 4 cut(s) 47, 275, 291, 546
FokI GGATG 2 cut(s) 482, 779
FriOI GRGCYC 2 cut(s) 184, 435
Fsp4HI GCNGC 4 cut(s) 47, 275, 291, 546
GlaI GCGC 1 cut(s) 278
GluI GCNGC 4 cut(s) 47, 275, 291, 546
GsuI CTGGAG 2 cut(s) 102, 233
HaeIII GGCC 3 cut(s) 182, 675, 707
HapII CCGG 2 cut(s) 191, 303
HhaI GCGC 1 cut(s) 279
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 3 cut(s) 199, 469, 647
Hin6I GCGC 1 cut(s) 277
HinP1I GCGC 1 cut(s) 277
HindIII AAGCTT 1 cut(s) 553
HinfI GANTC 1 cut(s) 248
HpaII CCGG 2 cut(s) 191, 303
HphI GGTGA 1 cut(s) 551
Hpy188I TCNGA 3 cut(s) 260, 316, 693
Hpy188III TCNNGA 6 cut(s) 212, 390, 492, 605, 619, 734
Hpy99I CGWCG 3 cut(s) 145, 148, 151
HpyAV CCTTC 2 cut(s) 172, 574
HpyCH4IV ACGT 4 cut(s) 62, 146, 529, 649
HpyCH4V TGCA 4 cut(s) 293, 328, 347, 533
HpyF10VI GCNNNNNNNGC 3 cut(s) 317, 344, 694
HpyF3I CTNAG 3 cut(s) 176, 639, 725
HpySE526I ACGT 4 cut(s) 62, 146, 529, 649
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 3 cut(s) 199, 469, 647
HspAI GCGC 1 cut(s) 277
Kzo9I GATC 3 cut(s) 26, 382, 386
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 54, 416, 430, 685
Lsp1109I GCAGC 4 cut(s) 58, 261, 277, 557
LweI GCATC 5 cut(s) 320, 463, 542, 564, 697
MaeII ACGT 4 cut(s) 62, 146, 529, 649
MalI GATC 3 cut(s) 28, 384, 388
MboI GATC 3 cut(s) 26, 382, 386
MboII GAAGA 8 cut(s) 34, 53, 96, 99, 102, 105, 239, 677
MhlI GDGCHC 3 cut(s) 184, 223, 435
MluCI AATT 4 cut(s) 93, 568, 680, 795
MmeI TCCRAC 1 cut(s) 486
MseI TTAA 1 cut(s) 281
MspCI CTTAAG 1 cut(s) 280
MspI CCGG 2 cut(s) 191, 303
MspR9I CCNGG 6 cut(s) 191, 335, 563, 677, 716, 830
Mva1269I GAATGC 1 cut(s) 236
MvaI CCWGG 5 cut(s) 335, 563, 677, 716, 830
MwoI GCNNNNNNNGC 3 cut(s) 317, 344, 694
NciI CCSGG 1 cut(s) 191
NdeII GATC 3 cut(s) 26, 382, 386
NlaIII CATG 3 cut(s) 199, 469, 647
NlaIV GGNNCC 2 cut(s) 182, 418
NspV TTCGAA 1 cut(s) 39
PasI CCCWGGG 1 cut(s) 829
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 155
PctI GAATGC 1 cut(s) 236
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 145
PflMI CCANNNNNTGG 2 cut(s) 211, 682
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 4 cut(s) 48, 276, 292, 547
PmaCI CACGTG 1 cut(s) 530
PmlI CACGTG 1 cut(s) 530
Ppu21I YACGTR 1 cut(s) 530
PsiI TTATAA 1 cut(s) 859
Psp124BI GAGCTC 1 cut(s) 435
Psp6I CCWGG 5 cut(s) 333, 561, 675, 714, 828
PspCI CACGTG 1 cut(s) 530
PspGI CCWGG 5 cut(s) 333, 561, 675, 714, 828
PspN4I GGNNCC 2 cut(s) 182, 418
PspOMI GGGCCC 1 cut(s) 180
PspPI GGNCC 3 cut(s) 180, 181, 706
PstNI CAGNNNCTG 1 cut(s) 211
PsyI GACNNNGTC 1 cut(s) 145
RsaI GTAC 2 cut(s) 123, 713
RsaNI GTAC 2 cut(s) 122, 712
SacI GAGCTC 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 281
SatI GCNGC 4 cut(s) 47, 275, 291, 546
Sau3AI GATC 3 cut(s) 26, 382, 386
Sau96I GGNCC 3 cut(s) 180, 181, 706
ScrFI CCNGG 6 cut(s) 191, 335, 563, 677, 716, 830
SduI GDGCHC 3 cut(s) 184, 223, 435
SfaNI GCATC 5 cut(s) 320, 463, 542, 564, 697
SfuI TTCGAA 1 cut(s) 39
SmlI CTYRAG 3 cut(s) 280, 500, 734
SmoI CTYRAG 3 cut(s) 280, 500, 734
Sse9I AATT 4 cut(s) 93, 568, 680, 795
SsiI CCGC 2 cut(s) 18, 234
SstI GAGCTC 1 cut(s) 435
StyD4I CCNGG 6 cut(s) 189, 333, 561, 675, 714, 828
TaiI ACGT 4 cut(s) 65, 149, 532, 652
TaqI TCGA 4 cut(s) 32, 39, 168, 385
TasI AATT 4 cut(s) 93, 568, 680, 795
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TseI GCWGC 4 cut(s) 46, 274, 290, 545
TspDTI ATGAA 3 cut(s) 154, 660, 678
Tth111I GACNNNGTC 1 cut(s) 145
Van91I CCANNNNNTGG 2 cut(s) 211, 682
Vha464I CTTAAG 1 cut(s) 280
XapI RAATTY 2 cut(s) 93, 568
ZraI GACGTC 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.