Rh2BG031500

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003445
Physical Location & Seq
Forward (+)
2099039 .. 2101530
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG031500.1

Sequence Viewer

Length: 822 bp
ATGTCTGCCGTAGTGTGCGGGAAGAGATCATCGATATTCGAAGAGCAGCTCCCCTCATCAACGTCATCGTCTCCTCCCGTTTCCTCCAAGAGAATTCGCTTTTCTTCTTCTTCTCCAGTACGCTTCTCCCCTCCCACGACGACGTCGTTTGGTTCGTCCTTCATCGACCAGCTCAGGGCCCTTTTCCCGGACATGGATAATCAGCTTCTGGAGAGAGCACTTGAAGAATGCGGTGAGGATTTGGATTCAGCTATCAGAAGTTTGAATGAGCTGCGCTTAAGTTCTGCTGCAAATAAATCCGGTGTAGCATCAGAAGCAAATGTGCAACCCCAGGCACAAGGTGCAGTAGCAACAAATGGAGAAGTTTCTGGTAATGATGATCTATCAGGACAAAATAACCTGCCCACGCAAGGAGCCGACTGGGTGGAGCTCTTTGTTAGAGAGATGATGAGTGCCTCCAACATGGATGATGCTAAAGCCCGTGCTTCAAGTGTGCTTGAGGTCTTGGAGAAATCCATCTGCTCACGTGCATCCACAGAGGCAGCCCAAAGCTTTCACCAGGAAAATTTGATGCTGAAGGAACAAATGGAAGCTCTTATTCAGGAAAATACAATCCTGAAGCGAGCTGTATCCACTCAGCATGAACGTCAAAAAGAGTATGAAGATAGAGGCCAGGAATTGGAGCATCTGAAGCAAGTGGTGGCCCAGTACCAGGAACAACTGAGAACTCTTGAGGTCAACAACTATGCACTTACGATGCACCTGAAGCAAGCTCAGCAAAGCAGCTCCATTCCGGGGCGATTTCACCCTGATGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

273

Amino Acids

30.14

Weight (kDa)

5.02

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 55 - 91 6.9e-06 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 408
AccB7I CCANNNNNTGG 1 cut(s) 679
AciI CCGC 2 cut(s) 18, 231
AcsI RAATTY 2 cut(s) 93, 565
AcuI CTGAAG 4 cut(s) 596, 638, 710, 785
AcvI CACGTG 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 341
AfaI GTAC 2 cut(s) 120, 710
AfiI CCNNNNNNNGG 7 cut(s) 175, 187, 193, 410, 679, 712, 795
AflII CTTAAG 1 cut(s) 277
AgsI TTSAA 3 cut(s) 224, 265, 489
AjnI CCWGG 4 cut(s) 330, 558, 672, 711
Alw21I GWGCWC 2 cut(s) 220, 432
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 208
AoxI GGCC 3 cut(s) 177, 670, 702
ApaI GGGCCC 1 cut(s) 181
ApeKI GCWGC 5 cut(s) 46, 271, 287, 542, 783
ApoI RAATTY 2 cut(s) 93, 565
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 3 cut(s) 177, 178, 703
AsuC2I CCSGG 2 cut(s) 188, 795
AsuHPI GGTGA 3 cut(s) 245, 548, 797
AsuII TTCGAA 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 181, 432
BbrPI CACGTG 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 220, 432
BbvI GCAGC 5 cut(s) 58, 258, 274, 554, 795
BccI CCATC 1 cut(s) 524
BciT130I CCWGG 4 cut(s) 332, 560, 674, 713
BciVI GTATCC 1 cut(s) 640
BcnI CCSGG 2 cut(s) 188, 795
BcoDI GTCTC 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 277
BfuAI ACCTGC 1 cut(s) 408
BfuI GTATCC 1 cut(s) 640
BisI GCNGC 5 cut(s) 47, 272, 288, 543, 784
BlpI GCTNAGC 1 cut(s) 774
BlsI GCNGC 5 cut(s) 48, 273, 289, 544, 785
Bme1390I CCNGG 6 cut(s) 188, 332, 560, 674, 713, 795
BmgT120I GGNCC 3 cut(s) 177, 178, 703
BmiI GGNNCC 2 cut(s) 179, 415
BmrFI CCNGG 6 cut(s) 188, 332, 560, 674, 713, 795
BmrI ACTGGG 2 cut(s) 430, 700
BmsI GCATC 6 cut(s) 317, 460, 539, 561, 694, 747
BmuI ACTGGG 2 cut(s) 430, 700
BpmI CTGGAG 2 cut(s) 99, 230
Bpu10I CCTNAGC 1 cut(s) 173
Bpu1102I GCTNAGC 1 cut(s) 774
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 518, 752
BpuMI CCSGG 2 cut(s) 188, 795
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 143
BsaJI CCNNGG 2 cut(s) 330, 794
BsaWI WCCGGW 1 cut(s) 299
Bsc4I CCNNNNNNNGG 7 cut(s) 175, 187, 193, 410, 679, 712, 795
Bse1I ACTGG 3 cut(s) 116, 425, 706
BseBI CCWGG 4 cut(s) 332, 560, 674, 713
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 330, 794
BseGI GGATG 2 cut(s) 472, 530
BseLI CCNNNNNNNGG 7 cut(s) 175, 187, 193, 410, 679, 712, 795
BseMII CTCAG 4 cut(s) 187, 650, 713, 788
BseNI ACTGG 3 cut(s) 116, 425, 706
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 5 cut(s) 58, 258, 274, 554, 795
BsgI GTGCAG 1 cut(s) 363
BshFI GGCC 3 cut(s) 179, 672, 704
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 220, 432
BsiSI CCGG 3 cut(s) 188, 300, 794
BslI CCNNNNNNNGG 7 cut(s) 175, 187, 193, 410, 679, 712, 795
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 233
BsnI GGCC 3 cut(s) 179, 672, 704
Bsp119I TTCGAA 1 cut(s) 39
Bsp120I GGGCCC 1 cut(s) 177
Bsp1286I GDGCHC 3 cut(s) 181, 220, 432
Bsp143I GATC 2 cut(s) 26, 379
Bsp1720I GCTNAGC 1 cut(s) 774
BspACI CCGC 2 cut(s) 18, 231
BspANI GGCC 3 cut(s) 179, 672, 704
BspCNI CTCAG 4 cut(s) 186, 649, 714, 787
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 179, 415
BspMI ACCTGC 1 cut(s) 408
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 277
BsrI ACTGG 3 cut(s) 116, 425, 706
BssECI CCNNGG 2 cut(s) 330, 794
BssMI GATC 2 cut(s) 26, 379
BssNI GRCGYC 1 cut(s) 143
Bst2UI CCWGG 4 cut(s) 332, 560, 674, 713
Bst6I CTCTTC 2 cut(s) 17, 36
BstACI GRCGYC 1 cut(s) 143
BstAFI CTTAAG 1 cut(s) 277
BstAPI GCANNNNNTGC 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 624, 771
BstDEI CTNAG 4 cut(s) 173, 636, 722, 774
BstF5I GGATG 2 cut(s) 472, 530
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 2 cut(s) 29, 382
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 26, 379
BstMWI GCNNNNNNNGC 5 cut(s) 314, 341, 691, 766, 775
BstNI CCWGG 4 cut(s) 332, 560, 674, 713
BstSCI CCNGG 6 cut(s) 186, 330, 558, 672, 711, 793
BstSLI GKGCMC 1 cut(s) 181
BstV1I GCAGC 5 cut(s) 58, 258, 274, 554, 795
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 3 cut(s) 179, 672, 704
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 472, 530
BveI ACCTGC 1 cut(s) 408
Cac8I GCNNGC 2 cut(s) 624, 771
CaiI CAGNNNCTG 1 cut(s) 208
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 3 cut(s) 177, 178, 703
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 119, 709
CviAII CATG 3 cut(s) 193, 463, 641
CviQI GTAC 2 cut(s) 119, 709
DdeI CTNAG 4 cut(s) 173, 636, 722, 774
DpnI GATC 2 cut(s) 28, 381
DpnII GATC 2 cut(s) 26, 379
DraIII CACNNNGTG 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 17, 36
EarI CTCTTC 2 cut(s) 17, 36
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 2 cut(s) 181, 432
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 4 cut(s) 596, 638, 710, 785
Eco72I CACGTG 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 2 cut(s) 177, 178
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 4 cut(s) 330, 558, 672, 711
EcoT38I GRGCYC 2 cut(s) 181, 432
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 3 cut(s) 196, 466, 644
FaiI YATR 5 cut(s) 194, 464, 642, 660, 747
FatI CATG 3 cut(s) 192, 462, 640
FauI CCCGC 1 cut(s) 11
Fnu4HI GCNGC 5 cut(s) 47, 272, 288, 543, 784
FokI GGATG 2 cut(s) 479, 517
FriOI GRGCYC 2 cut(s) 181, 432
Fsp4HI GCNGC 5 cut(s) 47, 272, 288, 543, 784
GlaI GCGC 1 cut(s) 275
GluI GCNGC 5 cut(s) 47, 272, 288, 543, 784
GsuI CTGGAG 2 cut(s) 99, 230
HaeIII GGCC 3 cut(s) 179, 672, 704
HapII CCGG 3 cut(s) 188, 300, 794
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 3 cut(s) 196, 466, 644
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 1 cut(s) 245
HpaII CCGG 3 cut(s) 188, 300, 794
HphI GGTGA 3 cut(s) 245, 548, 797
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 3 cut(s) 257, 313, 690
Hpy188III TCNNGA 5 cut(s) 209, 387, 602, 616, 731
Hpy8I GTNNAC 1 cut(s) 739
Hpy99I CGWCG 3 cut(s) 142, 145, 148
HpyAV CCTTC 2 cut(s) 169, 571
HpyCH4IV ACGT 4 cut(s) 62, 143, 526, 646
HpyCH4V TGCA 6 cut(s) 290, 325, 344, 530, 749, 760
HpyF10VI GCNNNNNNNGC 5 cut(s) 314, 341, 691, 766, 775
HpyF3I CTNAG 4 cut(s) 173, 636, 722, 774
HpySE526I ACGT 4 cut(s) 62, 143, 526, 646
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 3 cut(s) 196, 466, 644
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 26, 379
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 5 cut(s) 54, 413, 427, 682, 791
Lsp1109I GCAGC 5 cut(s) 58, 258, 274, 554, 795
LweI GCATC 6 cut(s) 317, 460, 539, 561, 694, 747
MaeII ACGT 4 cut(s) 62, 143, 526, 646
MalI GATC 2 cut(s) 28, 381
MboI GATC 2 cut(s) 26, 379
MboII GAAGA 7 cut(s) 34, 53, 96, 99, 102, 236, 674
MhlI GDGCHC 3 cut(s) 181, 220, 432
MluCI AATT 3 cut(s) 93, 565, 677
MmeI TCCRAC 1 cut(s) 483
MseI TTAA 1 cut(s) 278
MslI CAYNNNNRTG 1 cut(s) 810
MspCI CTTAAG 1 cut(s) 277
MspI CCGG 3 cut(s) 188, 300, 794
MspR9I CCNGG 6 cut(s) 188, 332, 560, 674, 713, 795
Mva1269I GAATGC 1 cut(s) 233
MvaI CCWGG 4 cut(s) 332, 560, 674, 713
MwoI GCNNNNNNNGC 5 cut(s) 314, 341, 691, 766, 775
NciI CCSGG 2 cut(s) 188, 795
NdeII GATC 2 cut(s) 26, 379
NlaIII CATG 3 cut(s) 196, 466, 644
NlaIV GGNNCC 2 cut(s) 179, 415
NspV TTCGAA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 152
PctI GAATGC 1 cut(s) 233
PfeI GAWTC 1 cut(s) 245
PflFI GACNNNGTC 1 cut(s) 142
PflMI CCANNNNNTGG 1 cut(s) 679
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 5 cut(s) 48, 273, 289, 544, 785
PmaCI CACGTG 1 cut(s) 527
PmlI CACGTG 1 cut(s) 527
Ppu21I YACGTR 1 cut(s) 527
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 4 cut(s) 330, 558, 672, 711
PspCI CACGTG 1 cut(s) 527
PspGI CCWGG 4 cut(s) 330, 558, 672, 711
PspN4I GGNNCC 2 cut(s) 179, 415
PspOMI GGGCCC 1 cut(s) 177
PspPI GGNCC 3 cut(s) 177, 178, 703
PstNI CAGNNNCTG 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 142
RsaI GTAC 2 cut(s) 120, 710
RsaNI GTAC 2 cut(s) 119, 709
RseI CAYNNNNRTG 1 cut(s) 810
SacI GAGCTC 1 cut(s) 432
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 5 cut(s) 47, 272, 288, 543, 784
Sau3AI GATC 2 cut(s) 26, 379
Sau96I GGNCC 3 cut(s) 177, 178, 703
ScrFI CCNGG 6 cut(s) 188, 332, 560, 674, 713, 795
SduI GDGCHC 3 cut(s) 181, 220, 432
SfaNI GCATC 6 cut(s) 317, 460, 539, 561, 694, 747
SfuI TTCGAA 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 810
SmlI CTYRAG 3 cut(s) 277, 497, 731
SmoI CTYRAG 3 cut(s) 277, 497, 731
Sse9I AATT 3 cut(s) 93, 565, 677
SsiI CCGC 2 cut(s) 18, 231
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 6 cut(s) 186, 330, 558, 672, 711, 793
TaiI ACGT 4 cut(s) 65, 146, 529, 649
TaqI TCGA 3 cut(s) 32, 39, 165
TasI AATT 3 cut(s) 93, 565, 677
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TseI GCWGC 5 cut(s) 46, 271, 287, 542, 783
TspDTI ATGAA 3 cut(s) 151, 657, 675
Tth111I GACNNNGTC 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 679
Vha464I CTTAAG 1 cut(s) 277
XapI RAATTY 2 cut(s) 93, 565
ZraI GACGTC 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.