RLG00000015893

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003445
Physical Location & Seq
Forward (+)
2149205 .. 2151055
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015893

Sequence Viewer

Length: 837 bp
ATGTCTGCCGTAGTGTGCGGGAAGAGATCATCGATATTCGAAGAGCAGCTCCCCTCATCAACGTCATCGTCTCCTCCCGTTTCCTCCAAGAGAATTCGCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCAGTACGCTTCTCCCCTCCCACGACGACGTCGTTTGGTTCGTCCTTCATCGACCAGCTCAGGGCCGTTTTCCCGGACATGGATAATCAGCTTCTGGAGAGAGCACTTGAAGAATGCGGTGATGATTTGGATTCAGCTATCAGAAGTTTGAATGAGCTGCGCTTAAGTTCTGCTGCAAATAAATCCGGTGTAGCATCAGAAGCAAATGTGCAACCCCAGGCACAAGGTGCAGTAGCAACAAATGGAGAAGTTTCTGGTAATGATGATCTATCAGGACAAAATAACCTGCCCACGCAAGGAGCTGACTGGGTGGAGCTCTTTGTTAGAGAGATGATGAGTGCCTCCAACATGGATGATGCTAAAGCCCGTGCTTCAAGAGTGCTTGAGGTCTTGGAGAAATCCATCTGCTCACGTGCATCCACAGAGGCAGCCCAAAGCTTTCACCAGGAAAATTTGATGCTGAAGGAACAAGTGGAAGCTCTTATTCAGGAAAATACAATCCTGAAGCGAGCTGTATCCACTCAGCATGAACGTCAAAAAGAGTATGAAGATAGAGGCCAGGAATTGGAGCATCTGAAGCAAGTCGTGGCCCAGTACCAGGAACAACTGAGAACTCTTGAGGTGAACAACTATGCACTTACGATGCACCTGAAGCAAGCTCAGCAAAGCAGCTCCATTCCAGGGCGATTTCACCCTGATGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

279

Amino Acids

30.59

Weight (kDa)

5.09

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 60 - 96 8.6e-06 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 423
AccB7I CCANNNNNTGG 1 cut(s) 694
AciI CCGC 2 cut(s) 18, 246
AcsI RAATTY 2 cut(s) 93, 580
AcuI CTGAAG 4 cut(s) 611, 653, 725, 800
AcvI CACGTG 1 cut(s) 542
AcyI GRCGYC 1 cut(s) 158
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 2 cut(s) 135, 725
AfiI CCNNNNNNNGG 6 cut(s) 190, 208, 425, 694, 727, 810
AflII CTTAAG 1 cut(s) 292
AgsI TTSAA 3 cut(s) 239, 280, 504
AjnI CCWGG 5 cut(s) 345, 573, 687, 726, 808
Alw21I GWGCWC 2 cut(s) 235, 447
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 223
AoxI GGCC 3 cut(s) 192, 685, 717
ApeKI GCWGC 5 cut(s) 46, 286, 302, 557, 798
ApoI RAATTY 2 cut(s) 93, 580
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 192, 718
AsuC2I CCSGG 1 cut(s) 203
AsuHPI GGTGA 4 cut(s) 260, 563, 763, 812
AsuII TTCGAA 1 cut(s) 39
BanII GRGCYC 1 cut(s) 447
BbrPI CACGTG 1 cut(s) 542
Bbv12I GWGCWC 2 cut(s) 235, 447
BbvI GCAGC 5 cut(s) 58, 273, 289, 569, 810
BccI CCATC 1 cut(s) 539
BceAI ACGGC 1 cut(s) 179
BciT130I CCWGG 5 cut(s) 347, 575, 689, 728, 810
BciVI GTATCC 1 cut(s) 655
BcnI CCSGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 423
BfuI GTATCC 1 cut(s) 655
BisI GCNGC 5 cut(s) 47, 287, 303, 558, 799
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 5 cut(s) 48, 288, 304, 559, 800
Bme1390I CCNGG 6 cut(s) 203, 347, 575, 689, 728, 810
BmgT120I GGNCC 2 cut(s) 192, 718
BmrFI CCNGG 6 cut(s) 203, 347, 575, 689, 728, 810
BmrI ACTGGG 2 cut(s) 445, 715
BmsI GCATC 6 cut(s) 332, 475, 554, 576, 709, 762
BmuI ACTGGG 2 cut(s) 445, 715
BpmI CTGGAG 2 cut(s) 114, 245
Bpu10I CCTNAGC 1 cut(s) 188
Bpu1102I GCTNAGC 1 cut(s) 789
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 533, 767
BpuMI CCSGG 1 cut(s) 203
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 542
BsaHI GRCGYC 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 345, 809
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 208, 425, 694, 727, 810
Bse1I ACTGG 3 cut(s) 131, 440, 721
BseBI CCWGG 5 cut(s) 347, 575, 689, 728, 810
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 345, 809
BseGI GGATG 2 cut(s) 487, 545
BseLI CCNNNNNNNGG 6 cut(s) 190, 208, 425, 694, 727, 810
BseMII CTCAG 4 cut(s) 202, 665, 728, 803
BseNI ACTGG 3 cut(s) 131, 440, 721
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 5 cut(s) 58, 273, 289, 569, 810
BsgI GTGCAG 1 cut(s) 378
BshFI GGCC 3 cut(s) 194, 687, 719
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 235, 447
BsiSI CCGG 2 cut(s) 203, 315
BslI CCNNNNNNNGG 6 cut(s) 190, 208, 425, 694, 727, 810
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 3 cut(s) 194, 687, 719
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 235, 447
Bsp143I GATC 2 cut(s) 26, 394
Bsp1720I GCTNAGC 1 cut(s) 789
BspACI CCGC 2 cut(s) 18, 246
BspANI GGCC 3 cut(s) 194, 687, 719
BspCNI CTCAG 4 cut(s) 201, 664, 729, 802
BspDI ATCGAT 1 cut(s) 32
BspMI ACCTGC 1 cut(s) 423
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 292
BsrI ACTGG 3 cut(s) 131, 440, 721
BssECI CCNNGG 2 cut(s) 345, 809
BssMI GATC 2 cut(s) 26, 394
BssNI GRCGYC 1 cut(s) 158
Bst2UI CCWGG 5 cut(s) 347, 575, 689, 728, 810
Bst6I CTCTTC 2 cut(s) 17, 36
BstACI GRCGYC 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 292
BstAPI GCANNNNNTGC 1 cut(s) 356
BstBAI YACGTR 1 cut(s) 542
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 639, 786
BstDEI CTNAG 4 cut(s) 188, 651, 737, 789
BstF5I GGATG 2 cut(s) 487, 545
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 2 cut(s) 29, 397
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 26, 394
BstMWI GCNNNNNNNGC 5 cut(s) 329, 356, 706, 781, 790
BstNI CCWGG 5 cut(s) 347, 575, 689, 728, 810
BstSCI CCNGG 6 cut(s) 201, 345, 573, 687, 726, 808
BstV1I GCAGC 5 cut(s) 58, 273, 289, 569, 810
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 3 cut(s) 194, 687, 719
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 487, 545
BveI ACCTGC 1 cut(s) 423
Cac8I GCNNGC 2 cut(s) 639, 786
CaiI CAGNNNCTG 1 cut(s) 223
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 2 cut(s) 192, 718
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 134, 724
CviAII CATG 3 cut(s) 208, 478, 656
CviQI GTAC 2 cut(s) 134, 724
DdeI CTNAG 4 cut(s) 188, 651, 737, 789
DpnI GATC 2 cut(s) 28, 396
DpnII GATC 2 cut(s) 26, 394
DraIII CACNNNGTG 1 cut(s) 356
Eam1104I CTCTTC 2 cut(s) 17, 36
EarI CTCTTC 2 cut(s) 17, 36
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 4 cut(s) 611, 653, 725, 800
Eco72I CACGTG 1 cut(s) 542
EcoICRI GAGCTC 1 cut(s) 445
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 5 cut(s) 345, 573, 687, 726, 808
EcoT38I GRGCYC 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 3 cut(s) 211, 481, 659
FaiI YATR 5 cut(s) 209, 479, 657, 675, 762
FatI CATG 3 cut(s) 207, 477, 655
FauI CCCGC 1 cut(s) 11
Fnu4HI GCNGC 5 cut(s) 47, 287, 303, 558, 799
FokI GGATG 2 cut(s) 494, 532
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 5 cut(s) 47, 287, 303, 558, 799
GlaI GCGC 1 cut(s) 290
GluI GCNGC 5 cut(s) 47, 287, 303, 558, 799
GsuI CTGGAG 2 cut(s) 114, 245
HaeIII GGCC 3 cut(s) 194, 687, 719
HapII CCGG 2 cut(s) 203, 315
HhaI GCGC 1 cut(s) 291
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 3 cut(s) 211, 481, 659
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 565
HinfI GANTC 1 cut(s) 260
HpaII CCGG 2 cut(s) 203, 315
HphI GGTGA 4 cut(s) 260, 563, 763, 812
Hpy166II GTNNAC 1 cut(s) 754
Hpy188I TCNGA 3 cut(s) 272, 328, 705
Hpy188III TCNNGA 6 cut(s) 224, 402, 504, 617, 631, 746
Hpy8I GTNNAC 1 cut(s) 754
Hpy99I CGWCG 3 cut(s) 157, 160, 163
HpyAV CCTTC 2 cut(s) 184, 586
HpyCH4IV ACGT 4 cut(s) 62, 158, 541, 661
HpyCH4V TGCA 6 cut(s) 305, 340, 359, 545, 764, 775
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 356, 706, 781, 790
HpyF3I CTNAG 4 cut(s) 188, 651, 737, 789
HpySE526I ACGT 4 cut(s) 62, 158, 541, 661
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 3 cut(s) 211, 481, 659
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 2 cut(s) 26, 394
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 5 cut(s) 54, 428, 442, 697, 806
Lsp1109I GCAGC 5 cut(s) 58, 273, 289, 569, 810
LweI GCATC 6 cut(s) 332, 475, 554, 576, 709, 762
MaeII ACGT 4 cut(s) 62, 158, 541, 661
MalI GATC 2 cut(s) 28, 396
MboI GATC 2 cut(s) 26, 394
MhlI GDGCHC 2 cut(s) 235, 447
MluCI AATT 3 cut(s) 93, 580, 692
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 9 cut(s) 64, 84, 94, 156, 481, 508, 547, 677, 742
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 825
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 2 cut(s) 203, 315
MspR9I CCNGG 6 cut(s) 203, 347, 575, 689, 728, 810
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 5 cut(s) 347, 575, 689, 728, 810
MwoI GCNNNNNNNGC 5 cut(s) 329, 356, 706, 781, 790
NciI CCSGG 1 cut(s) 203
NdeII GATC 2 cut(s) 26, 394
NlaIII CATG 3 cut(s) 211, 481, 659
NspV TTCGAA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 158, 167
PctI GAATGC 1 cut(s) 248
PfeI GAWTC 1 cut(s) 260
PflFI GACNNNGTC 1 cut(s) 157
PflMI CCANNNNNTGG 1 cut(s) 694
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 5 cut(s) 48, 288, 304, 559, 800
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
Ppu21I YACGTR 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 5 cut(s) 345, 573, 687, 726, 808
PspCI CACGTG 1 cut(s) 542
PspGI CCWGG 5 cut(s) 345, 573, 687, 726, 808
PspPI GGNCC 2 cut(s) 192, 718
PstNI CAGNNNCTG 1 cut(s) 223
PsyI GACNNNGTC 1 cut(s) 157
RsaI GTAC 2 cut(s) 135, 725
RsaNI GTAC 2 cut(s) 134, 724
RseI CAYNNNNRTG 1 cut(s) 825
SacI GAGCTC 1 cut(s) 447
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 5 cut(s) 47, 287, 303, 558, 799
Sau3AI GATC 2 cut(s) 26, 394
Sau96I GGNCC 2 cut(s) 192, 718
ScrFI CCNGG 6 cut(s) 203, 347, 575, 689, 728, 810
SduI GDGCHC 2 cut(s) 235, 447
SfaNI GCATC 6 cut(s) 332, 475, 554, 576, 709, 762
SfuI TTCGAA 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 3 cut(s) 292, 512, 746
SmoI CTYRAG 3 cut(s) 292, 512, 746
Sse9I AATT 3 cut(s) 93, 580, 692
SsiI CCGC 2 cut(s) 18, 246
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 6 cut(s) 201, 345, 573, 687, 726, 808
TaiI ACGT 4 cut(s) 65, 161, 544, 664
TaqI TCGA 3 cut(s) 32, 39, 180
TasI AATT 3 cut(s) 93, 580, 692
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TseI GCWGC 5 cut(s) 46, 286, 302, 557, 798
TspDTI ATGAA 3 cut(s) 166, 672, 690
Tth111I GACNNNGTC 1 cut(s) 157
Van91I CCANNNNNTGG 1 cut(s) 694
Vha464I CTTAAG 1 cut(s) 292
XapI RAATTY 2 cut(s) 93, 580
ZraI GACGTC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.