Rw2G002530

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003445
Physical Location & Seq
Forward (+)
2109889 .. 2112585
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002530.1

Sequence Viewer

Length: 768 bp
ATGTCTGCCGTAGTGTGCGGGAAGAGATCATCGATATTCGAAGAGCAGCTCCCCTCATCAACGTCATCGTCTCCTCCCGTTTCCTCCAAGAGAATTCGCTTTTCTTCTTCTTCTTCTTCTTCTCCGGTACGCTTCTCCCCTCCCACGACGACGTCGTTTGGTTCGTCCTTCATCGACCAGCTCAGGGCCGTTTTCCCGGACATGGATAATCAGCTTCTGGAGAGAGCACTTGAAGAATGCGGTAACGATTTGGATTCAGCTATCAGAAGTTTGAATGAGCTGCGCTTAAGTTCTGCTGCAAATAAATCCGGTGTAGCATCAGAAGCAAATGTGCAACCCCAGGCACAAGGTGCAGTAGCAACAAATGGAGAAGTTTCTGGTAATGATGATCTATCAGCACAAAATAACCTGCCCACGCAAGGAGCCGACTGGGTGGAGCTCTTTGTTAGAGAGATGATGAGTGCCTCCAACATGGATGATGCTAAAGCCCGTGCTTCAAGAGTGCTTGAGGTCTTGGAGAAATCCATCTGCTCACGTGCATCCACAGAGGCAGCCCAAAGCTTTCACCAGGAAAATTTGATGCTGAAGGAACAAGTGGAAGCTCTTATTCAGGAAAATACAATCCTGAAGCGAGCTGTATCCACTCAGCATGAACGTCAAAAAGAGTATGAAGATAGAGGCCAGGAATTGGAGCATCTGAAGCAAGTGGTGGCCCAGTACCAGGAACAACTGAGAACTCTTGAGGTTGAGCTTTATACATTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

255

Amino Acids

28.09

Weight (kDa)

4.88

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 58 - 94 7.2e-07 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 155
Acc36I ACCTGC 1 cut(s) 417
AccB7I CCANNNNNTGG 1 cut(s) 688
AciI CCGC 2 cut(s) 18, 240
AcsI RAATTY 2 cut(s) 93, 574
AcuI CTGAAG 3 cut(s) 605, 647, 719
AcvI CACGTG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 152
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 129, 719
AfiI CCNNNNNNNGG 5 cut(s) 184, 202, 419, 688, 721
AflII CTTAAG 1 cut(s) 286
AgsI TTSAA 3 cut(s) 233, 274, 498
AjnI CCWGG 4 cut(s) 339, 567, 681, 720
Alw21I GWGCWC 2 cut(s) 229, 441
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 217
AoxI GGCC 3 cut(s) 186, 679, 711
ApeKI GCWGC 4 cut(s) 46, 280, 296, 551
ApoI RAATTY 2 cut(s) 93, 574
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 2 cut(s) 186, 712
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 1 cut(s) 557
AsuII TTCGAA 1 cut(s) 39
BanII GRGCYC 1 cut(s) 441
BbrPI CACGTG 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 229, 441
BbvI GCAGC 4 cut(s) 58, 267, 283, 563
BccI CCATC 1 cut(s) 533
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 4 cut(s) 341, 569, 683, 722
BciVI GTATCC 1 cut(s) 649
BcnI CCSGG 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 75
BfrI CTTAAG 1 cut(s) 286
BfuAI ACCTGC 1 cut(s) 417
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 4 cut(s) 47, 281, 297, 552
BlsI GCNGC 4 cut(s) 48, 282, 298, 553
Bme1390I CCNGG 5 cut(s) 197, 341, 569, 683, 722
BmgT120I GGNCC 2 cut(s) 186, 712
BmiI GGNNCC 1 cut(s) 424
BmrFI CCNGG 5 cut(s) 197, 341, 569, 683, 722
BmrI ACTGGG 2 cut(s) 439, 709
BmsI GCATC 5 cut(s) 326, 469, 548, 570, 703
BmuI ACTGGG 2 cut(s) 439, 709
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 527, 761
BpuMI CCSGG 1 cut(s) 197
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 339
BsaWI WCCGGW 2 cut(s) 124, 308
Bsc4I CCNNNNNNNGG 5 cut(s) 184, 202, 419, 688, 721
Bse1I ACTGG 2 cut(s) 434, 715
BseBI CCWGG 4 cut(s) 341, 569, 683, 722
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 339
BseGI GGATG 2 cut(s) 481, 539
BseLI CCNNNNNNNGG 5 cut(s) 184, 202, 419, 688, 721
BseMII CTCAG 3 cut(s) 196, 659, 722
BseNI ACTGG 2 cut(s) 434, 715
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 58, 267, 283, 563
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 3 cut(s) 188, 681, 713
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 229, 441
BsiSI CCGG 3 cut(s) 125, 197, 309
BslI CCNNNNNNNGG 5 cut(s) 184, 202, 419, 688, 721
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 3 cut(s) 188, 681, 713
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 229, 441
Bsp143I GATC 2 cut(s) 26, 388
BspACI CCGC 2 cut(s) 18, 240
BspANI GGCC 3 cut(s) 188, 681, 713
BspCNI CTCAG 3 cut(s) 195, 658, 723
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 424
BspMI ACCTGC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BspTI CTTAAG 1 cut(s) 286
BsrI ACTGG 2 cut(s) 434, 715
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 2 cut(s) 26, 388
BssNI GRCGYC 1 cut(s) 152
Bst2UI CCWGG 4 cut(s) 341, 569, 683, 722
Bst6I CTCTTC 2 cut(s) 17, 36
BstACI GRCGYC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 350
BstBAI YACGTR 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 633
BstDEI CTNAG 3 cut(s) 182, 645, 731
BstF5I GGATG 2 cut(s) 481, 539
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 2 cut(s) 29, 391
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 26, 388
BstMWI GCNNNNNNNGC 3 cut(s) 323, 350, 700
BstNI CCWGG 4 cut(s) 341, 569, 683, 722
BstSCI CCNGG 5 cut(s) 195, 339, 567, 681, 720
BstV1I GCAGC 4 cut(s) 58, 267, 283, 563
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 3 cut(s) 188, 681, 713
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 481, 539
BveI ACCTGC 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 633
CaiI CAGNNNCTG 1 cut(s) 217
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 2 cut(s) 186, 712
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 128, 718
CviAII CATG 3 cut(s) 202, 472, 650
CviQI GTAC 2 cut(s) 128, 718
DdeI CTNAG 3 cut(s) 182, 645, 731
DpnI GATC 2 cut(s) 28, 390
DpnII GATC 2 cut(s) 26, 388
DraIII CACNNNGTG 1 cut(s) 350
Eam1104I CTCTTC 2 cut(s) 17, 36
EarI CTCTTC 2 cut(s) 17, 36
Ecl136II GAGCTC 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 3 cut(s) 605, 647, 719
Eco72I CACGTG 1 cut(s) 536
EcoICRI GAGCTC 1 cut(s) 439
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 4 cut(s) 339, 567, 681, 720
EcoT38I GRGCYC 1 cut(s) 441
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 3 cut(s) 205, 475, 653
FaiI YATR 6 cut(s) 203, 473, 651, 669, 756, 766
FatI CATG 3 cut(s) 201, 471, 649
FauI CCCGC 1 cut(s) 11
Fnu4HI GCNGC 4 cut(s) 47, 281, 297, 552
FokI GGATG 2 cut(s) 488, 526
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 4 cut(s) 47, 281, 297, 552
GlaI GCGC 1 cut(s) 284
GluI GCNGC 4 cut(s) 47, 281, 297, 552
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 3 cut(s) 188, 681, 713
HapII CCGG 3 cut(s) 125, 197, 309
HhaI GCGC 1 cut(s) 285
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 3 cut(s) 205, 475, 653
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 1 cut(s) 254
HpaII CCGG 3 cut(s) 125, 197, 309
HphI GGTGA 1 cut(s) 557
Hpy188I TCNGA 3 cut(s) 266, 322, 699
Hpy188III TCNNGA 5 cut(s) 218, 498, 611, 625, 740
Hpy99I CGWCG 3 cut(s) 151, 154, 157
HpyAV CCTTC 2 cut(s) 178, 580
HpyCH4IV ACGT 4 cut(s) 62, 152, 535, 655
HpyCH4V TGCA 4 cut(s) 299, 334, 353, 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 350, 700
HpyF3I CTNAG 3 cut(s) 182, 645, 731
HpySE526I ACGT 4 cut(s) 62, 152, 535, 655
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 3 cut(s) 205, 475, 653
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 26, 388
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 4 cut(s) 54, 422, 436, 691
Lsp1109I GCAGC 4 cut(s) 58, 267, 283, 563
LweI GCATC 5 cut(s) 326, 469, 548, 570, 703
MaeII ACGT 4 cut(s) 62, 152, 535, 655
MaeIII GTNAC 1 cut(s) 242
MalI GATC 2 cut(s) 28, 390
MboI GATC 2 cut(s) 26, 388
MhlI GDGCHC 2 cut(s) 229, 441
MluCI AATT 3 cut(s) 93, 574, 686
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 9 cut(s) 64, 84, 94, 150, 475, 502, 541, 671, 736
MseI TTAA 1 cut(s) 287
MspCI CTTAAG 1 cut(s) 286
MspI CCGG 3 cut(s) 125, 197, 309
MspR9I CCNGG 5 cut(s) 197, 341, 569, 683, 722
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 4 cut(s) 341, 569, 683, 722
MwoI GCNNNNNNNGC 3 cut(s) 323, 350, 700
NciI CCSGG 1 cut(s) 197
NdeII GATC 2 cut(s) 26, 388
NlaIII CATG 3 cut(s) 205, 475, 653
NlaIV GGNNCC 1 cut(s) 424
NspV TTCGAA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 152, 161
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 254
PflFI GACNNNGTC 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 688
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 4 cut(s) 48, 282, 298, 553
PmaCI CACGTG 1 cut(s) 536
PmlI CACGTG 1 cut(s) 536
Ppu21I YACGTR 1 cut(s) 536
Psp124BI GAGCTC 1 cut(s) 441
Psp6I CCWGG 4 cut(s) 339, 567, 681, 720
PspCI CACGTG 1 cut(s) 536
PspGI CCWGG 4 cut(s) 339, 567, 681, 720
PspN4I GGNNCC 1 cut(s) 424
PspPI GGNCC 2 cut(s) 186, 712
PstNI CAGNNNCTG 1 cut(s) 217
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 2 cut(s) 129, 719
RsaNI GTAC 2 cut(s) 128, 718
SacI GAGCTC 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 287
SatI GCNGC 4 cut(s) 47, 281, 297, 552
Sau3AI GATC 2 cut(s) 26, 388
Sau96I GGNCC 2 cut(s) 186, 712
ScrFI CCNGG 5 cut(s) 197, 341, 569, 683, 722
SduI GDGCHC 2 cut(s) 229, 441
SfaNI GCATC 5 cut(s) 326, 469, 548, 570, 703
SfuI TTCGAA 1 cut(s) 39
SmlI CTYRAG 3 cut(s) 286, 506, 740
SmoI CTYRAG 3 cut(s) 286, 506, 740
Sse9I AATT 3 cut(s) 93, 574, 686
SsiI CCGC 2 cut(s) 18, 240
SstI GAGCTC 1 cut(s) 441
StyD4I CCNGG 5 cut(s) 195, 339, 567, 681, 720
TaiI ACGT 4 cut(s) 65, 155, 538, 658
TaqI TCGA 3 cut(s) 32, 39, 174
TasI AATT 3 cut(s) 93, 574, 686
TfiI GAWTC 1 cut(s) 254
Tru1I TTAA 1 cut(s) 287
Tru9I TTAA 1 cut(s) 287
TseI GCWGC 4 cut(s) 46, 280, 296, 551
TspDTI ATGAA 3 cut(s) 160, 666, 684
Tth111I GACNNNGTC 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 688
Vha464I CTTAAG 1 cut(s) 286
XapI RAATTY 2 cut(s) 93, 574
ZraI GACGTC 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.