Rroxscaffold_2G00153280

Ubiquitin system component Cue protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003445
Physical Location & Seq
Reverse (-)
90047436 .. 90049939
2504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153280.1

Sequence Viewer

Length: 828 bp
ATGTCTGCCGTAGTGTGCGGGAAGAGATCATCGATATTCGAAGAGCAGCTCCCCTCATCAACGTCATCGTCTCCTCCCGTTTCCTCCAAGAGAATTCGCTTTTCTTCTTCTTCTTCTTCTCCAGTACGCTTCTCCCCTCCCACGACGACGTCGTTTCGTTCGTCCTTCATCGACCAGCTCAGGGCCGTTTTCCCGGACATGGATAATCAGCTTCTGGAGAGAGCACTTGAAGAATGCGGTGATGATTTGGATTCAGCTATCAGAAGTTTGAATGAGCTGCGGTTAAGTTCCGCTGCAAATAAATCCGGTGTAGCATCAGAAGCAAATGTGCAACCCCAGGCACAAGGTGCAGTAGCAACAAATGGACAAGTTTCTGGTAATGATGATCTATCAGGACAAAATAACCTGCCCACGCAAGGAGCCGACTGGGTGGAGCTCTTTGTTAGAGAGATGATGAGTGCCTCCAACATGGATGATGCTAAAGCCCGTGCTTCAAGAGTGCTTGAGGTCTTGGAGAAATCCATCTGCTCACGTGCATCCACAGAGGCAGCCCAAAGCTTTCACCAGGAAAATTTGATGCTGAAGGAACAAGTGGAAGCTCTTATTCAGGAAAATACAATCCTGAAGCGAGCTGTATCCACTCAGCATGAACGTCAAAAAGAGTATGAAGATAGAGGCCAGGAATTGGAGCATCTGAAGCAAGTGGTGGCCCAGTACCAGGAACAACTGAGAACTCTTGAGGTGAACAACTATGCACTTACGATGCACCTGAAGCAAGCTCAGCAAAGCAGCTCCATTCCGGGGCGATTTCACCCTGATGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

275

Amino Acids

30.43

Weight (kDa)

5.26

Isoelectric Point (pI)

60.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 57 - 93 7.4e-06 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 414
AccB7I CCANNNNNTGG 1 cut(s) 685
AciI CCGC 4 cut(s) 18, 237, 280, 291
AcsI RAATTY 2 cut(s) 93, 571
AcuI CTGAAG 4 cut(s) 602, 644, 716, 791
AcvI CACGTG 1 cut(s) 533
AcyI GRCGYC 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 347
AfaI GTAC 2 cut(s) 126, 716
AfiI CCNNNNNNNGG 6 cut(s) 181, 199, 416, 685, 718, 801
AgsI TTSAA 3 cut(s) 230, 271, 495
AjnI CCWGG 4 cut(s) 336, 564, 678, 717
Alw21I GWGCWC 2 cut(s) 226, 438
Alw26I GTCTC 1 cut(s) 75
AlwNI CAGNNNCTG 1 cut(s) 214
AoxI GGCC 3 cut(s) 183, 676, 708
ApeKI GCWGC 5 cut(s) 46, 277, 293, 548, 789
ApoI RAATTY 2 cut(s) 93, 571
AspS9I GGNCC 2 cut(s) 183, 709
AsuC2I CCSGG 2 cut(s) 194, 801
AsuHPI GGTGA 4 cut(s) 251, 554, 754, 803
AsuII TTCGAA 1 cut(s) 39
BanII GRGCYC 1 cut(s) 438
BbrPI CACGTG 1 cut(s) 533
Bbv12I GWGCWC 2 cut(s) 226, 438
BbvI GCAGC 5 cut(s) 58, 264, 280, 560, 801
BccI CCATC 1 cut(s) 530
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 4 cut(s) 338, 566, 680, 719
BciVI GTATCC 1 cut(s) 646
BcnI CCSGG 2 cut(s) 194, 801
BcoDI GTCTC 1 cut(s) 75
BfuAI ACCTGC 1 cut(s) 414
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 5 cut(s) 47, 278, 294, 549, 790
BlpI GCTNAGC 1 cut(s) 780
BlsI GCNGC 5 cut(s) 48, 279, 295, 550, 791
Bme1390I CCNGG 6 cut(s) 194, 338, 566, 680, 719, 801
BmgT120I GGNCC 2 cut(s) 183, 709
BmiI GGNNCC 1 cut(s) 421
BmrFI CCNGG 6 cut(s) 194, 338, 566, 680, 719, 801
BmrI ACTGGG 2 cut(s) 436, 706
BmsI GCATC 6 cut(s) 323, 466, 545, 567, 700, 753
BmuI ACTGGG 2 cut(s) 436, 706
BpmI CTGGAG 2 cut(s) 105, 236
Bpu10I CCTNAGC 1 cut(s) 179
Bpu1102I GCTNAGC 1 cut(s) 780
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 524, 758
BpuMI CCSGG 2 cut(s) 194, 801
Bsa29I ATCGAT 1 cut(s) 32
BsaAI YACGTR 1 cut(s) 533
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 336, 800
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 6 cut(s) 181, 199, 416, 685, 718, 801
Bse1I ACTGG 3 cut(s) 122, 431, 712
BseBI CCWGG 4 cut(s) 338, 566, 680, 719
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 2 cut(s) 336, 800
BseGI GGATG 2 cut(s) 478, 536
BseLI CCNNNNNNNGG 6 cut(s) 181, 199, 416, 685, 718, 801
BseMII CTCAG 4 cut(s) 193, 656, 719, 794
BseNI ACTGG 3 cut(s) 122, 431, 712
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 5 cut(s) 58, 264, 280, 560, 801
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 3 cut(s) 185, 678, 710
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 226, 438
BsiSI CCGG 3 cut(s) 194, 306, 800
BslI CCNNNNNNNGG 6 cut(s) 181, 199, 416, 685, 718, 801
BsmAI GTCTC 1 cut(s) 75
BsmBI CGTCTC 1 cut(s) 75
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 3 cut(s) 185, 678, 710
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 2 cut(s) 226, 438
Bsp143I GATC 2 cut(s) 26, 385
Bsp1720I GCTNAGC 1 cut(s) 780
BspACI CCGC 4 cut(s) 18, 237, 280, 291
BspANI GGCC 3 cut(s) 185, 678, 710
BspCNI CTCAG 4 cut(s) 192, 655, 720, 793
BspDI ATCGAT 1 cut(s) 32
BspLI GGNNCC 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 414
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 39
BsrI ACTGG 3 cut(s) 122, 431, 712
BssECI CCNNGG 2 cut(s) 336, 800
BssMI GATC 2 cut(s) 26, 385
BssNI GRCGYC 1 cut(s) 149
Bst2UI CCWGG 4 cut(s) 338, 566, 680, 719
Bst6I CTCTTC 2 cut(s) 17, 36
BstACI GRCGYC 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 347
BstBAI YACGTR 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 630, 777
BstDEI CTNAG 4 cut(s) 179, 642, 728, 780
BstF5I GGATG 2 cut(s) 478, 536
BstKTI GATC 2 cut(s) 29, 388
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 2 cut(s) 26, 385
BstMWI GCNNNNNNNGC 5 cut(s) 320, 347, 697, 772, 781
BstNI CCWGG 4 cut(s) 338, 566, 680, 719
BstSCI CCNGG 6 cut(s) 192, 336, 564, 678, 717, 799
BstV1I GCAGC 5 cut(s) 58, 264, 280, 560, 801
Bsu15I ATCGAT 1 cut(s) 32
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 3 cut(s) 185, 678, 710
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 2 cut(s) 478, 536
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 2 cut(s) 630, 777
CaiI CAGNNNCTG 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 183, 709
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 125, 715
CviAII CATG 3 cut(s) 199, 469, 647
CviQI GTAC 2 cut(s) 125, 715
DdeI CTNAG 4 cut(s) 179, 642, 728, 780
DpnI GATC 2 cut(s) 28, 387
DpnII GATC 2 cut(s) 26, 385
DraIII CACNNNGTG 1 cut(s) 347
Eam1104I CTCTTC 2 cut(s) 17, 36
EarI CTCTTC 2 cut(s) 17, 36
Ecl136II GAGCTC 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 438
Eco53kI GAGCTC 1 cut(s) 436
Eco57I CTGAAG 4 cut(s) 602, 644, 716, 791
Eco72I CACGTG 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 436
EcoRI GAATTC 1 cut(s) 93
EcoRII CCWGG 4 cut(s) 336, 564, 678, 717
EcoT38I GRGCYC 1 cut(s) 438
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 3 cut(s) 202, 472, 650
FaiI YATR 5 cut(s) 200, 470, 648, 666, 753
FatI CATG 3 cut(s) 198, 468, 646
FauI CCCGC 1 cut(s) 11
Fnu4HI GCNGC 5 cut(s) 47, 278, 294, 549, 790
FokI GGATG 2 cut(s) 485, 523
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 5 cut(s) 47, 278, 294, 549, 790
GluI GCNGC 5 cut(s) 47, 278, 294, 549, 790
GsuI CTGGAG 2 cut(s) 105, 236
HaeIII GGCC 3 cut(s) 185, 678, 710
HapII CCGG 3 cut(s) 194, 306, 800
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 3 cut(s) 202, 472, 650
HindIII AAGCTT 1 cut(s) 556
HinfI GANTC 1 cut(s) 251
HpaII CCGG 3 cut(s) 194, 306, 800
HphI GGTGA 4 cut(s) 251, 554, 754, 803
Hpy166II GTNNAC 1 cut(s) 745
Hpy188I TCNGA 3 cut(s) 263, 319, 696
Hpy188III TCNNGA 6 cut(s) 215, 393, 495, 608, 622, 737
Hpy8I GTNNAC 1 cut(s) 745
Hpy99I CGWCG 3 cut(s) 148, 151, 154
HpyAV CCTTC 2 cut(s) 175, 577
HpyCH4IV ACGT 4 cut(s) 62, 149, 532, 652
HpyCH4V TGCA 6 cut(s) 296, 331, 350, 536, 755, 766
HpyF10VI GCNNNNNNNGC 5 cut(s) 320, 347, 697, 772, 781
HpyF3I CTNAG 4 cut(s) 179, 642, 728, 780
HpySE526I ACGT 4 cut(s) 62, 149, 532, 652
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 3 cut(s) 202, 472, 650
Kzo9I GATC 2 cut(s) 26, 385
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 5 cut(s) 54, 419, 433, 688, 797
Lsp1109I GCAGC 5 cut(s) 58, 264, 280, 560, 801
LweI GCATC 6 cut(s) 323, 466, 545, 567, 700, 753
MaeII ACGT 4 cut(s) 62, 149, 532, 652
MalI GATC 2 cut(s) 28, 387
MboI GATC 2 cut(s) 26, 385
MboII GAAGA 9 cut(s) 34, 53, 96, 99, 102, 105, 108, 242, 680
MhlI GDGCHC 2 cut(s) 226, 438
MluCI AATT 3 cut(s) 93, 571, 683
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 9 cut(s) 64, 84, 94, 147, 472, 499, 538, 668, 733
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 1 cut(s) 816
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 3 cut(s) 194, 306, 800
MspR9I CCNGG 6 cut(s) 194, 338, 566, 680, 719, 801
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 4 cut(s) 338, 566, 680, 719
MwoI GCNNNNNNNGC 5 cut(s) 320, 347, 697, 772, 781
NciI CCSGG 2 cut(s) 194, 801
NdeII GATC 2 cut(s) 26, 385
NlaIII CATG 3 cut(s) 202, 472, 650
NlaIV GGNNCC 1 cut(s) 421
NspV TTCGAA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 2 cut(s) 149, 158
PctI GAATGC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 251
PflFI GACNNNGTC 1 cut(s) 148
PflMI CCANNNNNTGG 1 cut(s) 685
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 5 cut(s) 48, 279, 295, 550, 791
PmaCI CACGTG 1 cut(s) 533
PmlI CACGTG 1 cut(s) 533
Ppu21I YACGTR 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 4 cut(s) 336, 564, 678, 717
PspCI CACGTG 1 cut(s) 533
PspGI CCWGG 4 cut(s) 336, 564, 678, 717
PspN4I GGNNCC 1 cut(s) 421
PspPI GGNCC 2 cut(s) 183, 709
PstNI CAGNNNCTG 1 cut(s) 214
PsyI GACNNNGTC 1 cut(s) 148
RsaI GTAC 2 cut(s) 126, 716
RsaNI GTAC 2 cut(s) 125, 715
RseI CAYNNNNRTG 1 cut(s) 816
SacI GAGCTC 1 cut(s) 438
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 5 cut(s) 47, 278, 294, 549, 790
Sau3AI GATC 2 cut(s) 26, 385
Sau96I GGNCC 2 cut(s) 183, 709
ScrFI CCNGG 6 cut(s) 194, 338, 566, 680, 719, 801
SduI GDGCHC 2 cut(s) 226, 438
SfaNI GCATC 6 cut(s) 323, 466, 545, 567, 700, 753
SfuI TTCGAA 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 2 cut(s) 503, 737
SmoI CTYRAG 2 cut(s) 503, 737
Sse9I AATT 3 cut(s) 93, 571, 683
SsiI CCGC 4 cut(s) 18, 237, 280, 291
SstI GAGCTC 1 cut(s) 438
StyD4I CCNGG 6 cut(s) 192, 336, 564, 678, 717, 799
TaiI ACGT 4 cut(s) 65, 152, 535, 655
TaqI TCGA 3 cut(s) 32, 39, 171
TasI AATT 3 cut(s) 93, 571, 683
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TseI GCWGC 5 cut(s) 46, 277, 293, 548, 789
TspDTI ATGAA 3 cut(s) 157, 663, 681
Tth111I GACNNNGTC 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 685
XapI RAATTY 2 cut(s) 93, 571
ZraI GACGTC 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.