MD17G1213400.v1.1

CUE domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003445
Physical Location & Seq
Forward (+)
26042528 .. 26046136
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1213400.v1.1.491

Sequence Viewer

Length: 807 bp
ATGTCTGCGGCGGTGTGCGGAAGCAAGAGGTCTTTCTTCGACGATCAATCGCCGCCCCTCGCACAGAGGCTTCCTCCGTCGCCGGTCCTCACAAAGAGGCAGCGCCGTTCGTCTTCTACCTCTCCCATTCGCTTCGCTGCTCCGTATCTTCTCGAGCAGCTTCGCTCTCTTTTCCCTCACATGGACCCTCAGGTTCTTGAGAGAGCATTGGAAGAATGTGGCAACGATATGGATGCTGCCATCAAGAGGCTGCATGAACTTTGCCTAGGATCTGCGGAGGAAACTTCTGCATCTGCCGAACAAACAGCTGATATCGTAGCTAACGGTCTATTGTGTAATGATGAAGCTGCAGCCACTACTGAGAATCCATCAGTTCCAAACAACCTTCCTGCAGATGGTGCAGAATGGGTTGAGTTGTTTGTAAGGGAAATGATGAGTGCCAGTAGTGTGGATGATGCTAGGGCCCGTGCTGCTAAAGTTCTAGAGGTTTTAGAAAAATCCATCAGTGAACGTGCTGGTGCTGAGGCAGCCCAAAGTTTTCAGAAGGAAAATATGATGCTGAAACAACAAATTGAAGGATTGGTAAGGGAGAATACCATACTGAAACGTGCTGTAGCTATCCAGCATGAACGTCAAAAAGAATTTGAAGAGAGGAACCAAGAATTACAGCATCTGAAGCAGCTAGTTTCTCAGTATCAGGAGCAGTGTAGAACACTAGAGATTAACAACTATGCTCTGACAATCCGTCTGAAGCAGGCGGAGCAAAGCAACTCCATCCCTGGACGTTTCCACCCGGACATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CUE

Protein Analysis

269

Amino Acids

30.01

Weight (kDa)

5.32

Isoelectric Point (pI)

61.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CUE PF02845 51 - 84 4.1e-09 CUE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 8, 11, 18, 53, 275, 758
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 2 cut(s) 695, 770
AfiI CCNNNNNNNGG 3 cut(s) 181, 246, 395
AgsI TTSAA 2 cut(s) 575, 647
AhdI GACNNNNNGTC 1 cut(s) 744
AjnI CCWGG 1 cut(s) 778
AluBI AGCT 6 cut(s) 160, 308, 320, 347, 617, 682
AluI AGCT 6 cut(s) 160, 308, 320, 347, 617, 682
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 673
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 462
ApaI GGGCCC 1 cut(s) 466
ApoI RAATTY 1 cut(s) 641
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 4 cut(s) 85, 184, 462, 463
AsuC2I CCSGG 1 cut(s) 794
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 2 cut(s) 85, 184
AvrII CCTAGG 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 522
BccI CCATC 5 cut(s) 248, 376, 389, 509, 782
BceAI ACGGC 1 cut(s) 90
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 780
BcnI CCSGG 1 cut(s) 794
BfaI CTAG 5 cut(s) 266, 459, 482, 683, 716
BfmI CTRYAG 3 cut(s) 348, 390, 612
BfoI RGCGCY 1 cut(s) 106
BlnI CCTAGG 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 780, 794
Bme18I GGWCC 2 cut(s) 85, 184
BmeRI GACNNNNNGTC 1 cut(s) 744
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 4 cut(s) 85, 184, 462, 463
BmiI GGNNCC 3 cut(s) 186, 464, 656
BmrFI CCNGG 2 cut(s) 780, 794
BmsI GCATC 5 cut(s) 223, 299, 445, 546, 679
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 58, 90
Bpu10I CCTNAGC 1 cut(s) 522
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 794
BsaJI CCNNGG 2 cut(s) 265, 778
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 246, 395
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 441
Bse21I CCTNAGG 1 cut(s) 189
BseBI CCWGG 1 cut(s) 780
BseDI CCNNGG 2 cut(s) 265, 778
BseGI GGATG 3 cut(s) 238, 457, 774
BseLI CCNNNNNNNGG 3 cut(s) 181, 246, 395
BseMII CTCAG 4 cut(s) 203, 351, 513, 704
BseNI ACTGG 1 cut(s) 441
BseSI GKGCMC 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 1 cut(s) 464
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 2 cut(s) 83, 794
BslI CCNNNNNNNGG 3 cut(s) 181, 246, 395
BsnI GGCC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 152
Bsp120I GGGCCC 1 cut(s) 462
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 2 cut(s) 43, 269
BspACI CCGC 6 cut(s) 8, 11, 18, 53, 275, 758
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 4 cut(s) 202, 352, 514, 703
BspLI GGNNCC 3 cut(s) 186, 464, 656
BspMAI CTGCAG 2 cut(s) 352, 394
BspPI GGATC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 441
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 265, 778
BssMI GATC 2 cut(s) 43, 269
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 1 cut(s) 326
Bst6I CTCTTC 1 cut(s) 642
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 756
BstDEI CTNAG 4 cut(s) 189, 360, 522, 690
BstF5I GGATG 3 cut(s) 238, 457, 774
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 46, 272
BstMBI GATC 2 cut(s) 43, 269
BstMWI GCNNNNNNNGC 5 cut(s) 398, 470, 527, 676, 760
BstNI CCWGG 1 cut(s) 780
BstSCI CCNGG 2 cut(s) 778, 792
BstSFI CTRYAG 3 cut(s) 348, 390, 612
BstSLI GKGCMC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 269
BstXI CCANNNNNNTGG 1 cut(s) 448
BstYI RGATCY 1 cut(s) 269
Bsu36I CCTNAGG 1 cut(s) 189
BsuRI GGCC 1 cut(s) 464
BtsCI GGATG 3 cut(s) 238, 457, 774
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 2 cut(s) 511, 710
Cac8I GCNNGC 1 cut(s) 756
CaiI CAGNNNCTG 1 cut(s) 673
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 4 cut(s) 85, 184, 462, 463
CviAII CATG 3 cut(s) 181, 254, 626
DdeI CTNAG 4 cut(s) 189, 360, 522, 690
DpnI GATC 2 cut(s) 45, 271
DpnII GATC 2 cut(s) 43, 269
DriI GACNNNNNGTC 1 cut(s) 744
Eam1104I CTCTTC 1 cut(s) 642
Eam1105I GACNNNNNGTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 773
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 466
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 85, 184
Eco57I CTGAAG 2 cut(s) 695, 770
Eco81I CCTNAGG 1 cut(s) 189
Eco88I CYCGRG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 778
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 3 cut(s) 184, 257, 629
FaiI YATR 7 cut(s) 182, 230, 255, 554, 599, 627, 732
FatI CATG 3 cut(s) 180, 253, 625
FokI GGATG 3 cut(s) 245, 464, 761
FriOI GRGCYC 1 cut(s) 466
FspBI CTAG 5 cut(s) 266, 459, 482, 683, 716
GlaI GCGC 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 1 cut(s) 464
HapII CCGG 2 cut(s) 83, 794
HhaI GCGC 1 cut(s) 105
Hin1II CATG 3 cut(s) 184, 257, 629
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 1 cut(s) 364
HpaII CCGG 2 cut(s) 83, 794
Hpy166II GTNNAC 1 cut(s) 509
Hpy188I TCNGA 4 cut(s) 543, 675, 738, 750
Hpy188III TCNNGA 5 cut(s) 152, 197, 244, 482, 698
Hpy8I GTNNAC 1 cut(s) 509
Hpy99I CGWCG 2 cut(s) 44, 82
HpyAV CCTTC 3 cut(s) 395, 538, 569
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 4 cut(s) 511, 607, 631, 784
HpyCH4V TGCA 5 cut(s) 253, 290, 350, 392, 401
HpyF10VI GCNNNNNNNGC 5 cut(s) 398, 470, 527, 676, 760
HpyF3I CTNAG 4 cut(s) 189, 360, 522, 690
HpySE526I ACGT 4 cut(s) 511, 607, 631, 784
Hsp92II CATG 3 cut(s) 184, 257, 629
HspAI GCGC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 43, 269
LmnI GCTCC 3 cut(s) 145, 700, 760
LweI GCATC 5 cut(s) 223, 299, 445, 546, 679
MaeI CTAG 5 cut(s) 266, 459, 482, 683, 716
MaeII ACGT 4 cut(s) 511, 607, 631, 784
MalI GATC 2 cut(s) 45, 271
MboI GATC 2 cut(s) 43, 269
MboII GAAGA 5 cut(s) 28, 105, 140, 224, 659
MflI RGATCY 1 cut(s) 269
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 3 cut(s) 570, 641, 662
MseI TTAA 2 cut(s) 723, 805
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 2 cut(s) 83, 794
MspR9I CCNGG 2 cut(s) 780, 794
MvaI CCWGG 1 cut(s) 780
MwoI GCNNNNNNNGC 5 cut(s) 398, 470, 527, 676, 760
NciI CCSGG 1 cut(s) 794
NdeII GATC 2 cut(s) 43, 269
NlaIII CATG 3 cut(s) 184, 257, 629
NlaIV GGNNCC 3 cut(s) 186, 464, 656
PaeR7I CTCGAG 1 cut(s) 152
PfeI GAWTC 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 778
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 3 cut(s) 186, 464, 656
PspOMI GGGCCC 1 cut(s) 462
PspPI GGNCC 4 cut(s) 85, 184, 462, 463
PstI CTGCAG 2 cut(s) 352, 394
PstNI CAGNNNCTG 1 cut(s) 673
PsuI RGATCY 1 cut(s) 269
PvuII CAGCTG 1 cut(s) 308
SaqAI TTAA 2 cut(s) 723, 805
Sau3AI GATC 2 cut(s) 43, 269
Sau96I GGNCC 4 cut(s) 85, 184, 462, 463
ScrFI CCNGG 2 cut(s) 780, 794
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 5 cut(s) 223, 299, 445, 546, 679
SfcI CTRYAG 3 cut(s) 348, 390, 612
Sfr274I CTCGAG 1 cut(s) 152
SinI GGWCC 2 cut(s) 85, 184
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 152, 197
SmoI CTYRAG 2 cut(s) 152, 197
Sse9I AATT 3 cut(s) 570, 641, 662
SsiI CCGC 6 cut(s) 8, 11, 18, 53, 275, 758
SspMI CTAG 5 cut(s) 266, 459, 482, 683, 716
StyD4I CCNGG 2 cut(s) 778, 792
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 4 cut(s) 514, 610, 634, 787
TaqI TCGA 2 cut(s) 39, 153
TasI AATT 3 cut(s) 570, 641, 662
TauI GCSGC 2 cut(s) 11, 55
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 723, 805
Tru9I TTAA 2 cut(s) 723, 805
TscAI CASTG 2 cut(s) 511, 710
TspDTI ATGAA 3 cut(s) 270, 357, 642
TspGWI ACGGA 3 cut(s) 66, 132, 734
TspRI CASTG 2 cut(s) 511, 710
VpaK11BI GGWCC 2 cut(s) 85, 184
XapI RAATTY 1 cut(s) 641
XbaI TCTAGA 1 cut(s) 481
XhoI CTCGAG 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 265
XspI CTAG 5 cut(s) 266, 459, 482, 683, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.