FvH4_6g54290

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006057
Physical Location & Seq
Forward (+)
39677376 .. 39679252
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54290.t1

Sequence Viewer

Length: 1272 bp
ATGGAAGATAGTTTCAGCGTTCGAGTAGGCAAAACCTTCGATATTCTGGCGGGTGCATCATCGGCGAGGGTTTCGTCTTCATCTCTGAGCTCGCTGTGGTCGCTGACAGGTGAGGAAATCGAGAAGAGAGAGTGGAACAGGGACAGGGGAAGCCCCGAACCCCAACTCCGACCTTGCGATCCGGAATCAAAGGATGATTTTTTCGGTAGCGAGCTGGAGAAGGACCTTCTAGACCTTGACGACGGCGATGACGTGGAAGAAGAAGAAGAAGAGGAGCAATCTGCTAAGCCTACGGCTATGCCGGATGGTTATGACGAGGAGCAGTGGCAGATTAAAAACTGCATTGGCTTGGACCCTACTCTCGACCACGAGGAAGAGGAAGATGAATTTGACAAAGTGGCAGTTGGTAAGGGAAATATTGGATATCGCTTTTATATGACGGATGTGAATGACTATCCCATAGATATTGATTCTCAATACGAGGTTCCCAATTCAGTGGAGGATTTTACTCCAGACCCACGTGCCAATCATCTAGCAGCTCAGATTAGACTCAGACAGGATGCAGAAGCAGCTGCTCAAATTAGGCTCCAAGAGGGTGCTGAAGCTGCTGGGAACAGTGCATCTGGTGCTATAGCTGATGAGTCCATCACGTCTGAAGATGCTATCAGCCTGAAATCAATTTTGAAGAGAAAGAATGATGAGCAGTCAGGCTCATCTAAATCACGAAAGCGTGTTCGGTTTGACCCTCAGTGTAAAGATGATTGTGATGAAGAGCCTGATGGATCCATAGATTTACCTGTCAAAAGCCTTTCAGGTGAAGATCATGCGTCGGCAGTTCCAGATTACATCCGGAATCCATCAAGATATACACATTACACATTTGATTCATTACCTGACGTGGACGAGGAAGCTAACAGGCAAGCCTATATGGATTTCCGTAATCTTCTGGGGCAGTCAAATACTATGGAAGCAGAAGCAGAAGCAGAAGCACCAAGGGATTTTTCAAAACCAGTCATATTCACACCAAAAAAGAAATCAACTGATGCGACAATGCTAGAAATTGATGGGGAGTTGGAGAGACCAGTAGGTGCTAGCAAGGAATTTCCGCTTTACAAAGTCATGCCAATTGCAATTGCAGCCAGGGAACAAGAAGACAGTGATGTGTGTGCAATGGACGAAGATGAACCGGAGACAGAAAGCAGTGTAAGAACAAGTGTACAGAGGAGTGGCCGCCAGTTTCGTACAAGGACCAGTTTAGAGTTGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

424

Amino Acids

47.33

Weight (kDa)

4.31

Isoelectric Point (pI)

59.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 203 - 323 6.6e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 181, 849
AciI CCGC 3 cut(s) 50, 1106, 1231
AclWI GGATC 3 cut(s) 173, 777, 790
AcoI YGGCCR 1 cut(s) 1228
AcsI RAATTY 2 cut(s) 386, 1100
AcuI CTGAAG 2 cut(s) 621, 675
AcvI CACGTG 1 cut(s) 521
AfaI GTAC 2 cut(s) 1218, 1243
AgsI TTSAA 2 cut(s) 685, 1005
AjiI CACGTC 3 cut(s) 253, 651, 898
AjnI CCWGG 1 cut(s) 1139
AjuI GAANNNNNNNTTGG 2 cut(s) 985, 1017
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 7 cut(s) 90, 214, 539, 572, 605, 635, 911
AluI AGCT 7 cut(s) 90, 214, 539, 572, 605, 635, 911
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 2 cut(s) 1072, 1184
AlwI GGATC 3 cut(s) 173, 777, 790
Aor13HI TCCGGA 2 cut(s) 181, 849
AoxI GGCC 1 cut(s) 1228
ApeKI GCWGC 5 cut(s) 536, 569, 572, 605, 1136
ApoI RAATTY 2 cut(s) 386, 1100
AspS9I GGNCC 3 cut(s) 223, 352, 1248
AsuHPI GGTGA 2 cut(s) 122, 827
AsuNHI GCTAGC 1 cut(s) 1091
AvaII GGWCC 3 cut(s) 223, 352, 1248
BamHI GGATCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 92
BauI CACGAG 1 cut(s) 368
BbrPI CACGTG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 69, 1158
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 5 cut(s) 548, 559, 581, 592, 1148
BccI CCATC 5 cut(s) 299, 653, 773, 865, 1058
BceAI ACGGC 2 cut(s) 259, 309
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 1 cut(s) 1141
BcoDI GTCTC 2 cut(s) 1072, 1184
BfaI CTAG 4 cut(s) 230, 533, 1055, 1092
BfmI CTRYAG 1 cut(s) 630
BisI GCNGC 6 cut(s) 537, 570, 573, 606, 1137, 1231
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 6 cut(s) 538, 571, 574, 607, 1138, 1232
Bme1390I CCNGG 1 cut(s) 1141
Bme18I GGWCC 3 cut(s) 223, 352, 1248
BmgBI CACGTC 3 cut(s) 253, 651, 898
BmgT120I GGNCC 3 cut(s) 223, 352, 1248
BmiI GGNNCC 4 cut(s) 354, 486, 587, 784
BmrFI CCNGG 1 cut(s) 1141
BmsI GCATC 5 cut(s) 65, 550, 629, 649, 1033
BmtI GCTAGC 1 cut(s) 1095
BpiI GAAGAC 2 cut(s) 69, 1158
BpmI CTGGAG 2 cut(s) 236, 495
Bpu1102I GCTNAGC 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 521
BsaI GGTCTC 1 cut(s) 1072
BsaJI CCNNGG 2 cut(s) 992, 1140
BsaWI WCCGGW 3 cut(s) 181, 849, 1186
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bse1I ACTGG 4 cut(s) 1010, 1082, 1234, 1251
Bse3DI GCAATG 1 cut(s) 1176
BseAI TCCGGA 2 cut(s) 181, 849
BseBI CCWGG 1 cut(s) 1141
BseDI CCNNGG 2 cut(s) 992, 1140
BseGI GGATG 6 cut(s) 199, 310, 448, 565, 846, 1270
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 4 cut(s) 77, 554, 565, 761
BseNI ACTGG 4 cut(s) 1010, 1082, 1234, 1251
BseRI GAGGAG 3 cut(s) 287, 332, 1237
BseXI GCAGC 5 cut(s) 548, 559, 581, 592, 1148
BseYI CCCAGC 1 cut(s) 608
BshFI GGCC 1 cut(s) 1230
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 4 cut(s) 182, 302, 850, 1187
BslFI GGGAC 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 1072, 1184
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 1 cut(s) 1230
Bso31I GGTCTC 1 cut(s) 1072
Bsp1286I GDGCHC 1 cut(s) 92
Bsp13I TCCGGA 2 cut(s) 181, 849
Bsp1407I TGTACA 1 cut(s) 1216
Bsp143I GATC 3 cut(s) 178, 782, 820
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 3 cut(s) 50, 1106, 1231
BspANI GGCC 1 cut(s) 1230
BspCNI CTCAG 4 cut(s) 78, 553, 564, 760
BspEI TCCGGA 2 cut(s) 181, 849
BspLI GGNNCC 4 cut(s) 354, 486, 587, 784
BspOI GCTAGC 1 cut(s) 1095
BspPI GGATC 3 cut(s) 173, 777, 790
BspQI GCTCTTC 1 cut(s) 765
BspTNI GGTCTC 1 cut(s) 1072
BsrDI GCAATG 1 cut(s) 1176
BsrGI TGTACA 1 cut(s) 1216
BsrI ACTGG 4 cut(s) 1010, 1082, 1234, 1251
BssECI CCNNGG 2 cut(s) 992, 1140
BssMI GATC 3 cut(s) 178, 782, 820
BssSI CACGAG 1 cut(s) 368
BssT1I CCWWGG 1 cut(s) 992
Bst2BI CACGAG 1 cut(s) 368
Bst2UI CCWGG 1 cut(s) 1141
Bst4CI ACNGT 2 cut(s) 617, 1157
Bst6I CTCTTC 5 cut(s) 119, 264, 369, 680, 765
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 1216
BstBAI YACGTR 1 cut(s) 521
BstC8I GCNNGC 4 cut(s) 92, 212, 921, 1093
BstDEI CTNAG 5 cut(s) 86, 285, 540, 551, 747
BstF5I GGATG 6 cut(s) 199, 310, 448, 565, 846, 1270
BstKTI GATC 3 cut(s) 181, 785, 823
BstMAI GTCTC 2 cut(s) 1072, 1184
BstMBI GATC 3 cut(s) 178, 782, 820
BstMWI GCNNNNNNNGC 6 cut(s) 62, 100, 569, 605, 626, 1136
BstNI CCWGG 1 cut(s) 1141
BstSCI CCNGG 1 cut(s) 1139
BstSFI CTRYAG 1 cut(s) 630
BstV1I GCAGC 5 cut(s) 548, 559, 581, 592, 1148
BstV2I GAAGAC 2 cut(s) 69, 1158
BstX2I RGATCY 1 cut(s) 782
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 1 cut(s) 782
BsuRI GGCC 1 cut(s) 1230
BtgZI GCGATG 1 cut(s) 261
BtrI CACGTC 3 cut(s) 253, 651, 898
BtsCI GGATG 6 cut(s) 199, 310, 448, 565, 846, 1270
BtsI GCAGTG 2 cut(s) 329, 1207
BtsIMutI CAGTG 6 cut(s) 329, 501, 622, 755, 1162, 1207
Cac8I GCNNGC 4 cut(s) 92, 212, 921, 1093
Cfr13I GGNCC 3 cut(s) 223, 352, 1248
CseI GACGC 1 cut(s) 816
Csp6I GTAC 2 cut(s) 1217, 1242
CviAII CATG 2 cut(s) 824, 1120
CviQI GTAC 2 cut(s) 1217, 1242
DdeI CTNAG 5 cut(s) 86, 285, 540, 551, 747
DpnI GATC 3 cut(s) 180, 784, 822
DpnII GATC 3 cut(s) 178, 782, 820
EaeI YGGCCR 1 cut(s) 1228
Eam1104I CTCTTC 5 cut(s) 119, 264, 369, 680, 765
EarI CTCTTC 5 cut(s) 119, 264, 369, 680, 765
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 992
Eco24I GRGCYC 1 cut(s) 92
Eco31I GGTCTC 1 cut(s) 1072
Eco32I GATATC 1 cut(s) 425
Eco47I GGWCC 3 cut(s) 223, 352, 1248
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 621, 675
Eco72I CACGTG 1 cut(s) 521
EcoICRI GAGCTC 1 cut(s) 90
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 1139
EcoRV GATATC 1 cut(s) 425
EcoT14I CCWWGG 1 cut(s) 992
EcoT38I GRGCYC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 992
FaeI CATG 2 cut(s) 827, 1123
FaqI GGGAC 1 cut(s) 155
FatI CATG 2 cut(s) 823, 1119
FauI CCCGC 1 cut(s) 43
Fnu4HI GCNGC 6 cut(s) 537, 570, 573, 606, 1137, 1231
FokI GGATG 5 cut(s) 206, 317, 455, 572, 833
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 6 cut(s) 537, 570, 573, 606, 1137, 1231
FspBI CTAG 4 cut(s) 230, 533, 1055, 1092
GluI GCNGC 6 cut(s) 537, 570, 573, 606, 1137, 1231
GsaI CCCAGC 1 cut(s) 612
GsuI CTGGAG 2 cut(s) 236, 495
HaeIII GGCC 1 cut(s) 1230
HapII CCGG 4 cut(s) 182, 302, 850, 1187
HgaI GACGC 1 cut(s) 816
Hin1II CATG 2 cut(s) 827, 1123
HinfI GANTC 6 cut(s) 185, 470, 549, 641, 853, 884
HpaII CCGG 4 cut(s) 182, 302, 850, 1187
HphI GGTGA 2 cut(s) 122, 827
Hpy166II GTNNAC 2 cut(s) 901, 1217
Hpy188I TCNGA 5 cut(s) 87, 170, 543, 554, 655
Hpy188III TCNNGA 9 cut(s) 121, 182, 230, 362, 512, 723, 839, 850, 861
Hpy8I GTNNAC 2 cut(s) 901, 1217
Hpy99I CGWCG 2 cut(s) 245, 832
HpyAV CCTTC 3 cut(s) 46, 214, 236
HpyCH4III ACNGT 2 cut(s) 617, 1157
HpyCH4IV ACGT 4 cut(s) 252, 520, 650, 897
HpyCH4V TGCA 7 cut(s) 56, 342, 563, 620, 1130, 1136, 1169
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 100, 569, 605, 626, 1136
HpyF3I CTNAG 5 cut(s) 86, 285, 540, 551, 747
HpySE526I ACGT 4 cut(s) 252, 520, 650, 897
Hsp92II CATG 2 cut(s) 827, 1123
Kpn2I TCCGGA 2 cut(s) 181, 849
Kzo9I GATC 3 cut(s) 178, 782, 820
LguI GCTCTTC 1 cut(s) 765
LmnI GCTCC 3 cut(s) 274, 319, 591
Lsp1109I GCAGC 5 cut(s) 548, 559, 581, 592, 1148
LweI GCATC 5 cut(s) 65, 550, 629, 649, 1033
MaeI CTAG 4 cut(s) 230, 533, 1055, 1092
MaeII ACGT 4 cut(s) 252, 520, 650, 897
MalI GATC 3 cut(s) 180, 784, 822
MboI GATC 3 cut(s) 178, 782, 820
MfeI CAATTG 2 cut(s) 1125, 1131
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 8 cut(s) 386, 490, 579, 678, 1059, 1100, 1125, 1131
MlyI GAGTC 2 cut(s) 543, 650
MmeI TCCRAC 3 cut(s) 193, 1053, 1242
MroI TCCGGA 2 cut(s) 181, 849
MseI TTAA 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 4 cut(s) 182, 302, 850, 1187
MspR9I CCNGG 1 cut(s) 1141
MunI CAATTG 2 cut(s) 1125, 1131
MvaI CCWGG 1 cut(s) 1141
MwoI GCNNNNNNNGC 6 cut(s) 62, 100, 569, 605, 626, 1136
NdeII GATC 3 cut(s) 178, 782, 820
NheI GCTAGC 1 cut(s) 1091
NlaIII CATG 2 cut(s) 827, 1123
NlaIV GGNNCC 4 cut(s) 354, 486, 587, 784
PciSI GCTCTTC 1 cut(s) 765
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 4 cut(s) 185, 470, 853, 884
PkrI GCNGC 6 cut(s) 538, 571, 574, 607, 1138, 1232
PleI GAGTC 2 cut(s) 543, 649
PmaCI CACGTG 1 cut(s) 521
PmlI CACGTG 1 cut(s) 521
PpsI GAGTC 2 cut(s) 543, 649
Ppu21I YACGTR 1 cut(s) 521
PpuMI RGGWCCY 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 1139
PspCI CACGTG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 608
PspGI CCWGG 1 cut(s) 1139
PspN4I GGNNCC 4 cut(s) 354, 486, 587, 784
PspPI GGNCC 3 cut(s) 223, 352, 1248
PspPPI RGGWCCY 1 cut(s) 223
PsuI RGATCY 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 2 cut(s) 1218, 1243
RsaNI GTAC 2 cut(s) 1217, 1242
SacI GAGCTC 1 cut(s) 92
SapI GCTCTTC 1 cut(s) 765
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 6 cut(s) 537, 570, 573, 606, 1137, 1231
Sau3AI GATC 3 cut(s) 178, 782, 820
Sau96I GGNCC 3 cut(s) 223, 352, 1248
SchI GAGTC 2 cut(s) 543, 650
ScrFI CCNGG 1 cut(s) 1141
SduI GDGCHC 1 cut(s) 92
SfaNI GCATC 5 cut(s) 65, 550, 629, 649, 1033
SfcI CTRYAG 1 cut(s) 630
SinI GGWCC 3 cut(s) 223, 352, 1248
Sse9I AATT 8 cut(s) 386, 490, 579, 678, 1059, 1100, 1125, 1131
SsiI CCGC 3 cut(s) 50, 1106, 1231
SspI AATATT 1 cut(s) 418
SspMI CTAG 4 cut(s) 230, 533, 1055, 1092
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 1 cut(s) 1139
StyI CCWWGG 1 cut(s) 992
TaaI ACNGT 2 cut(s) 617, 1157
TaiI ACGT 4 cut(s) 255, 523, 653, 900
TaqI TCGA 4 cut(s) 22, 39, 120, 363
TasI AATT 8 cut(s) 386, 490, 579, 678, 1059, 1100, 1125, 1131
TatI WGTACW 1 cut(s) 1216
TauI GCSGC 1 cut(s) 1233
TfiI GAWTC 4 cut(s) 185, 470, 853, 884
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 6 cut(s) 329, 501, 622, 755, 1162, 1207
TseI GCWGC 5 cut(s) 536, 569, 572, 605, 1136
TspDTI ATGAA 5 cut(s) 69, 399, 783, 876, 1197
TspGWI ACGGA 2 cut(s) 455, 926
TspRI CASTG 6 cut(s) 329, 501, 622, 755, 1162, 1207
VpaK11BI GGWCC 3 cut(s) 223, 352, 1248
XapI RAATTY 2 cut(s) 386, 1100
XbaI TCTAGA 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 1 cut(s) 1258
XspI CTAG 4 cut(s) 230, 533, 1055, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.