Rh3CG226300

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0006057
Physical Location & Seq
Reverse (-)
21245910 .. 21247088
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG226300.1

Sequence Viewer

Length: 1023 bp
ATGCAAGACAGTTTCAGCTTCCGAGTAGGCAAGACCTTCGACTCTCTACCTGGTTCATGGTCCCTTGGAGATGAGAAAATCGTTAGAAGGGAATGTAACAGGAACAATGAGAGCCCTGAACCCGAATCCGAGCTCCACCAATACCACACATATTCAACAGATGACTTCTGTAACGAGCTGGAGAATGGCCTCCTAGAATTTTTTGACGACAAGTTCTTAGTACAGGAAGAAGAACGTGGTTGGTTCGGTCAATCTTTCGAGTCTAAAGCCAACCCTAAGACTAAGCCAGATGATTATGAAGAGGAGCAGTGGGAAATTAAAACCTCTATTGGCTTAGACCTCACACTCGACTCCGAGGACGAGGAAGATGGATACGATAAATTGGCTGTTGGAATGGAAGACTCTGTAGATCGTCCTTATATGAATGAGTACGTAAACGACGACGATGAGACAGACAGTGATGAGGTTCCCAATTTGCTCGAGGGTTTCTCTGGAGACCCACTTGAAATTGGCATAAAATCGCTTTTGAAGAGGACGAGTATTGATGATCAATTAGTCTCATCTACAGTAGAAAAACGTGTTCGATTTCGAGACGAAGAGGAGCATGAAGGATCGAAAGATCGTTGGTCTGCAGCAGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATATGTTTGATTCATCTAGTGACATGGATGACAAATCTAACGAGCAAGCCTATTTGAACTTCCGTATGCAGTTTTCGAGATTGATGAAGTCAGATACTATGGAAGCCGAGGAAGTTGCTTGTATTGCTGTTTCAAAACCAGTCGCATTGGTACAAAGAAAGATATCAACTGATGCTAGTAAGGAACTTGCGCCTTGCAGAATCAGGCCAATTGCTTTTCCAGACACTGATGATTGTGAGATGGAGGAAGATGAACAGGTGACAGGATCAAGCAGTGGAAGTGCCGGCGTGCGGAGAACTATCCCCCGCCAGTTTTGTACAAACACCAGCTTAGAGTTGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

340

Amino Acids

39.04

Weight (kDa)

4.32

Isoelectric Point (pI)

50.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 206 - 256 8e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 967, 982
AclWI GGATC 2 cut(s) 619, 949
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 4 cut(s) 222, 431, 828, 994
AfiI CCNNNNNNNGG 1 cut(s) 966
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 5 cut(s) 156, 506, 529, 733, 810
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 4 cut(s) 18, 133, 178, 1005
AluI AGCT 4 cut(s) 18, 133, 178, 1005
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 4 cut(s) 443, 489, 562, 585
AlwI GGATC 2 cut(s) 619, 949
AlwNI CAGNNNCTG 1 cut(s) 902
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 2 cut(s) 187, 881
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 1 cut(s) 197
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 946
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 60
BanII GRGCYC 2 cut(s) 116, 135
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 644
BccI CCATC 3 cut(s) 362, 667, 910
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 773, 807
BciT130I CCWGG 1 cut(s) 51
BciVI GTATCC 1 cut(s) 365
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 4 cut(s) 443, 489, 562, 585
BfaI CTAG 3 cut(s) 194, 693, 852
BfmI CTRYAG 3 cut(s) 405, 564, 630
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 1 cut(s) 633
BlsI GCNGC 1 cut(s) 634
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 2 cut(s) 62, 468
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 2 cut(s) 200, 513
BsaAI YACGTR 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 3 cut(s) 64, 354, 783
Bsc4I CCNNNNNNNGG 1 cut(s) 966
Bse118I RCCGGY 1 cut(s) 959
Bse1I ACTGG 2 cut(s) 815, 985
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 3 cut(s) 64, 354, 783
BseGI GGATG 2 cut(s) 709, 1021
BseLI CCNNNNNNNGG 1 cut(s) 966
BseNI ACTGG 2 cut(s) 815, 985
BseRI GAGGAG 2 cut(s) 317, 614
BseXI GCAGC 1 cut(s) 644
BshFI GGCC 2 cut(s) 189, 883
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 1 cut(s) 960
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 966
BsmAI GTCTC 4 cut(s) 443, 489, 562, 585
BsmBI CGTCTC 1 cut(s) 585
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 2 cut(s) 189, 883
Bso31I GGTCTC 1 cut(s) 489
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 2 cut(s) 116, 135
Bsp1407I TGTACA 1 cut(s) 992
Bsp143I GATC 5 cut(s) 409, 547, 611, 619, 941
BspACI CCGC 2 cut(s) 967, 982
BspANI GGCC 2 cut(s) 189, 883
BspLI GGNNCC 2 cut(s) 62, 468
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 2 cut(s) 619, 949
BspTNI GGTCTC 1 cut(s) 489
BsrFI RCCGGY 1 cut(s) 959
BsrGI TGTACA 1 cut(s) 992
BsrI ACTGG 2 cut(s) 815, 985
BssAI RCCGGY 1 cut(s) 959
BssECI CCNNGG 3 cut(s) 64, 354, 783
BssMI GATC 5 cut(s) 409, 547, 611, 619, 941
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 11, 458, 568
Bst6I CTCTTC 3 cut(s) 294, 524, 591
BstAUI TGTACA 1 cut(s) 992
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 723, 961, 965
BstDEI CTNAG 5 cut(s) 217, 276, 282, 334, 1006
BstF5I GGATG 2 cut(s) 709, 1021
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 5 cut(s) 412, 550, 614, 622, 944
BstMAI GTCTC 4 cut(s) 443, 489, 562, 585
BstMBI GATC 5 cut(s) 409, 547, 611, 619, 941
BstMWI GCNNNNNNNGC 1 cut(s) 800
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstSFI CTRYAG 3 cut(s) 405, 564, 630
BstSNI TACGTA 1 cut(s) 433
BstV1I GCAGC 1 cut(s) 644
BstV2I GAAGAC 1 cut(s) 405
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 189, 883
BtsCI GGATG 2 cut(s) 709, 1021
BtsI GCAGTG 2 cut(s) 314, 955
BtsIMutI CAGTG 4 cut(s) 314, 463, 900, 955
Cac8I GCNNGC 3 cut(s) 723, 961, 965
CaiI CAGNNNCTG 1 cut(s) 902
CfoI GCGC 1 cut(s) 868
Cfr10I RCCGGY 1 cut(s) 959
Cfr13I GGNCC 1 cut(s) 60
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 4 cut(s) 221, 430, 827, 993
CviAII CATG 3 cut(s) 57, 605, 700
CviQI GTAC 4 cut(s) 221, 430, 827, 993
DdeI CTNAG 5 cut(s) 217, 276, 282, 334, 1006
DpnI GATC 5 cut(s) 411, 549, 613, 621, 943
DpnII GATC 5 cut(s) 409, 547, 611, 619, 941
Eam1104I CTCTTC 3 cut(s) 294, 524, 591
EarI CTCTTC 3 cut(s) 294, 524, 591
Ecl136II GAGCTC 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 116, 135
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 49
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 64
EcoT38I GRGCYC 2 cut(s) 116, 135
ErhI CCWWGG 1 cut(s) 64
Esp3I CGTCTC 1 cut(s) 585
FaeI CATG 3 cut(s) 60, 608, 703
FaqI GGGAC 1 cut(s) 46
FatI CATG 3 cut(s) 56, 604, 699
FauI CCCGC 1 cut(s) 989
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 1 cut(s) 633
FokI GGATG 1 cut(s) 716
FriOI GRGCYC 2 cut(s) 116, 135
Fsp4HI GCNGC 1 cut(s) 633
FspBI CTAG 3 cut(s) 194, 693, 852
GlaI GCGC 1 cut(s) 867
GluI GCNGC 1 cut(s) 633
GsuI CTGGAG 2 cut(s) 200, 513
HaeIII GGCC 2 cut(s) 189, 883
HapII CCGG 1 cut(s) 960
HhaI GCGC 1 cut(s) 868
Hin1II CATG 3 cut(s) 60, 608, 703
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HinfI GANTC 8 cut(s) 41, 125, 260, 350, 401, 655, 686, 876
HpaII CCGG 1 cut(s) 960
HphI GGTGA 1 cut(s) 946
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 4 cut(s) 23, 130, 355, 769
Hpy188III TCNNGA 6 cut(s) 492, 590, 641, 663, 753, 896
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 2 cut(s) 443, 446
HpyAV CCTTC 3 cut(s) 46, 81, 602
HpyCH4III ACNGT 3 cut(s) 11, 458, 568
HpyCH4IV ACGT 3 cut(s) 235, 432, 577
HpyCH4V TGCA 4 cut(s) 4, 632, 745, 873
HpyF10VI GCNNNNNNNGC 1 cut(s) 800
HpyF3I CTNAG 5 cut(s) 217, 276, 282, 334, 1006
HpySE526I ACGT 3 cut(s) 235, 432, 577
Hsp92II CATG 3 cut(s) 60, 608, 703
HspAI GCGC 1 cut(s) 866
KroI GCCGGC 1 cut(s) 959
KroNI GCCGGC 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 5 cut(s) 409, 547, 611, 619, 941
LmnI GCTCC 3 cut(s) 138, 304, 601
Lsp1109I GCAGC 1 cut(s) 644
LweI GCATC 1 cut(s) 838
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 3 cut(s) 194, 693, 852
MaeII ACGT 3 cut(s) 235, 432, 577
MaeIII GTNAC 4 cut(s) 95, 170, 695, 934
MalI GATC 5 cut(s) 411, 549, 613, 621, 943
MboI GATC 5 cut(s) 409, 547, 611, 619, 941
MboII GAAGA 8 cut(s) 239, 242, 311, 377, 410, 541, 608, 935
MfeI CAATTG 1 cut(s) 885
MhlI GDGCHC 2 cut(s) 116, 135
MluCI AATT 7 cut(s) 197, 315, 380, 472, 507, 551, 885
MlyI GAGTC 4 cut(s) 35, 269, 344, 395
MmeI TCCRAC 2 cut(s) 370, 993
MroNI GCCGGC 1 cut(s) 959
MseI TTAA 1 cut(s) 318
MspI CCGG 1 cut(s) 960
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 1 cut(s) 800
NaeI GCCGGC 1 cut(s) 961
NdeII GATC 5 cut(s) 409, 547, 611, 619, 941
NgoMIV GCCGGC 1 cut(s) 959
NlaIII CATG 3 cut(s) 60, 608, 703
NlaIV GGNNCC 2 cut(s) 62, 468
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 2 cut(s) 695, 934
PaeR7I CTCGAG 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 961
PfeI GAWTC 4 cut(s) 125, 655, 686, 876
PkrI GCNGC 1 cut(s) 634
PleI GAGTC 4 cut(s) 35, 268, 344, 395
PpsI GAGTC 4 cut(s) 35, 268, 344, 395
Ppu21I YACGTR 1 cut(s) 433
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 62, 468
PspPI GGNCC 1 cut(s) 60
PspXI VCTCGAGB 1 cut(s) 479
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 902
RsaI GTAC 4 cut(s) 222, 431, 828, 994
RsaNI GTAC 4 cut(s) 221, 430, 827, 993
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 1 cut(s) 633
Sau3AI GATC 5 cut(s) 409, 547, 611, 619, 941
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 4 cut(s) 35, 269, 344, 395
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 2 cut(s) 116, 135
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 1 cut(s) 838
SfcI CTRYAG 3 cut(s) 405, 564, 630
Sfr274I CTCGAG 1 cut(s) 479
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SnaBI TACGTA 1 cut(s) 433
Sse9I AATT 7 cut(s) 197, 315, 380, 472, 507, 551, 885
SsiI CCGC 2 cut(s) 967, 982
SspMI CTAG 3 cut(s) 194, 693, 852
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 11, 458, 568
TaiI ACGT 3 cut(s) 238, 435, 580
TaqI TCGA 8 cut(s) 39, 258, 348, 480, 583, 589, 614, 752
TaqII GACCGA 1 cut(s) 236
TasI AATT 7 cut(s) 197, 315, 380, 472, 507, 551, 885
TatI WGTACW 2 cut(s) 220, 992
TfiI GAWTC 4 cut(s) 125, 655, 686, 876
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 4 cut(s) 314, 463, 907, 955
TseFI GTSAC 2 cut(s) 695, 934
TseI GCWGC 1 cut(s) 632
Tsp45I GTSAC 2 cut(s) 695, 934
TspDTI ATGAA 7 cut(s) 45, 312, 437, 621, 678, 776, 942
TspGWI ACGGA 2 cut(s) 667, 728
TspRI CASTG 4 cut(s) 314, 463, 907, 955
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 3 cut(s) 194, 693, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.