RchiOBHm_Chr3g0475791

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0006057
Physical Location & Seq
Reverse (-)
21612425 .. 21613438
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44126

Sequence Viewer

Length: 1014 bp
ATGCAAGACAGTTTCAGCTTCCGAGTAGGCAAGACCTTCGACTCTCTACCTGGTTCATGGTCCCTTGGAGATGAGAAAATCGATAGAAGGGAATGTAACAGGAACAATGAGAGCCCTGAACCCGAATCCGAGCTCCACCGATACCACACATATTCAACGGATGACTTCTGTAACGAGCTGGAGAATGGCCTCCTAGAATTTTTTGACGACAAGTTCTTAGTACAGGAAGAAGAACGTGGTTGGTTCGGTCAATCTTTCGAGTCTAAAGCCAACCCTAAGACTAAGCCAGATGATTATGAAGAGGAGCAGTGGGAAATTAAAACCTCTATTGGCTTAGACCTCACACTCGACTCCGAGGACGAGGAAGATGGATACGATAAATTGGCTGTTGGAATGGAAGACTCTGTAGATCGTCCTGATATGAATGAGTACGTAAACGACGACGATGAGACAGACAGTGATGAGGTTCCCAATTTGCTCAAGGGTTTCTCTGGAGACCCACTTGAAATTGGCATAAAATCGCTTTTGAAGAGGACGAGTATTGATGATCAATTAGTCTCATCTACAGTAGAAAAACGTGTTCGATTTCGAGACGAAGAGGAGCATGAAGGATCGAAAGATCGTTGGGCTGCAGCAGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATATGTTTGATTCATCTAGTGACATGGATGACAAATCTAACGAGCAAGCCTATTTGAACTTCCGTATGCAATTTTCGAGATTGATGAAGTCAGATACTATGGAAGCCGAGGAAGTTGCTTGTATTGCTGTTTCAAAACCAGTCGCATTGGTACAAAGAAAGATATCAACTGATGCTAGTAAGGAACTTGCGCCTTGCAGAATCAGGCCAATTGCTTTTCCAGACACTGATGATTGTGAGATGGAGGAAGATGAACCGGTGACAGGATCAAGCAGTGGACCGGAAGAGCCGGCGTGCGGAGAACTATCCCCCGCCAATTTCGTACAAACACCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

337

Amino Acids

38.45

Weight (kDa)

4.27

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 206 - 256 8.2e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 972, 987
AclWI GGATC 2 cut(s) 619, 949
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 4 cut(s) 222, 431, 828, 999
AfiI CCNNNNNNNGG 2 cut(s) 938, 971
AflIII ACRYGT 1 cut(s) 577
AgeI ACCGGT 1 cut(s) 931
AgsI TTSAA 5 cut(s) 156, 506, 529, 733, 810
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 4 cut(s) 18, 133, 178, 1010
AluI AGCT 4 cut(s) 18, 133, 178, 1010
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 4 cut(s) 443, 489, 562, 585
AlwI GGATC 2 cut(s) 619, 949
AlwNI CAGNNNCTG 1 cut(s) 902
AoxI GGCC 2 cut(s) 187, 881
ApeKI GCWGC 2 cut(s) 629, 632
ApoI RAATTY 1 cut(s) 197
AsiGI ACCGGT 1 cut(s) 931
AspLEI GCGC 1 cut(s) 868
AspS9I GGNCC 2 cut(s) 60, 953
AsuHPI GGTGA 1 cut(s) 946
AvaII GGWCC 2 cut(s) 60, 953
BanII GRGCYC 2 cut(s) 116, 135
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 616, 644
BccI CCATC 3 cut(s) 362, 667, 910
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 773, 807
BciT130I CCWGG 1 cut(s) 51
BciVI GTATCC 1 cut(s) 365
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 4 cut(s) 443, 489, 562, 585
BfaI CTAG 3 cut(s) 194, 693, 852
BfmI CTRYAG 3 cut(s) 405, 564, 630
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 2 cut(s) 630, 633
BlsI GCNGC 2 cut(s) 631, 634
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 2 cut(s) 60, 953
BmgT120I GGNCC 2 cut(s) 60, 953
BmiI GGNNCC 2 cut(s) 62, 468
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 405
BpmI CTGGAG 2 cut(s) 200, 513
BpuEI CTTGAG 1 cut(s) 464
Bsa29I ATCGAT 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 3 cut(s) 64, 354, 783
BsaWI WCCGGW 2 cut(s) 931, 955
Bsc4I CCNNNNNNNGG 2 cut(s) 938, 971
Bse118I RCCGGY 2 cut(s) 931, 964
Bse1I ACTGG 1 cut(s) 815
BseBI CCWGG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 64, 354, 783
BseGI GGATG 2 cut(s) 166, 709
BseLI CCNNNNNNNGG 2 cut(s) 938, 971
BseNI ACTGG 1 cut(s) 815
BseRI GAGGAG 2 cut(s) 317, 614
BseXI GCAGC 2 cut(s) 616, 644
BshFI GGCC 2 cut(s) 189, 883
BshTI ACCGGT 1 cut(s) 931
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 3 cut(s) 932, 956, 965
BslFI GGGAC 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 938, 971
BsmAI GTCTC 4 cut(s) 443, 489, 562, 585
BsmBI CGTCTC 1 cut(s) 585
BsmFI GGGAC 1 cut(s) 46
BsnI GGCC 2 cut(s) 189, 883
Bso31I GGTCTC 1 cut(s) 489
Bsp1286I GDGCHC 2 cut(s) 116, 135
Bsp143I GATC 5 cut(s) 409, 547, 611, 619, 941
BspACI CCGC 2 cut(s) 972, 987
BspANI GGCC 2 cut(s) 189, 883
BspDI ATCGAT 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 62, 468
BspMAI CTGCAG 1 cut(s) 634
BspPI GGATC 2 cut(s) 619, 949
BspQI GCTCTTC 1 cut(s) 954
BspTNI GGTCTC 1 cut(s) 489
BsrFI RCCGGY 2 cut(s) 931, 964
BsrI ACTGG 1 cut(s) 815
BssAI RCCGGY 2 cut(s) 931, 964
BssECI CCNNGG 3 cut(s) 64, 354, 783
BssMI GATC 5 cut(s) 409, 547, 611, 619, 941
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 11, 458, 568
Bst6I CTCTTC 4 cut(s) 294, 524, 591, 954
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 3 cut(s) 723, 966, 970
BstDEI CTNAG 5 cut(s) 217, 276, 282, 334, 1011
BstF5I GGATG 2 cut(s) 166, 709
BstHHI GCGC 1 cut(s) 868
BstKTI GATC 5 cut(s) 412, 550, 614, 622, 944
BstMAI GTCTC 4 cut(s) 443, 489, 562, 585
BstMBI GATC 5 cut(s) 409, 547, 611, 619, 941
BstMWI GCNNNNNNNGC 1 cut(s) 800
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstSFI CTRYAG 3 cut(s) 405, 564, 630
BstSNI TACGTA 1 cut(s) 433
BstV1I GCAGC 2 cut(s) 616, 644
BstV2I GAAGAC 1 cut(s) 405
Bsu15I ATCGAT 1 cut(s) 81
BsuI GTATCC 1 cut(s) 365
BsuRI GGCC 2 cut(s) 189, 883
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 2 cut(s) 166, 709
BtsI GCAGTG 2 cut(s) 314, 955
BtsIMutI CAGTG 4 cut(s) 314, 463, 900, 955
Cac8I GCNNGC 3 cut(s) 723, 966, 970
CaiI CAGNNNCTG 1 cut(s) 902
CfoI GCGC 1 cut(s) 868
Cfr10I RCCGGY 2 cut(s) 931, 964
Cfr13I GGNCC 2 cut(s) 60, 953
ClaI ATCGAT 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 4 cut(s) 221, 430, 827, 998
CspAI ACCGGT 1 cut(s) 931
CviAII CATG 3 cut(s) 57, 605, 700
CviQI GTAC 4 cut(s) 221, 430, 827, 998
DdeI CTNAG 5 cut(s) 217, 276, 282, 334, 1011
DpnI GATC 5 cut(s) 411, 549, 613, 621, 943
DpnII GATC 5 cut(s) 409, 547, 611, 619, 941
Eam1104I CTCTTC 4 cut(s) 294, 524, 591, 954
EarI CTCTTC 4 cut(s) 294, 524, 591, 954
Ecl136II GAGCTC 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 116, 135
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 2 cut(s) 60, 953
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 49
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 64
EcoT38I GRGCYC 2 cut(s) 116, 135
ErhI CCWWGG 1 cut(s) 64
Esp3I CGTCTC 1 cut(s) 585
FaeI CATG 3 cut(s) 60, 608, 703
FaqI GGGAC 1 cut(s) 46
FatI CATG 3 cut(s) 56, 604, 699
FauI CCCGC 1 cut(s) 994
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 630, 633
FokI GGATG 2 cut(s) 173, 716
FriOI GRGCYC 2 cut(s) 116, 135
Fsp4HI GCNGC 2 cut(s) 630, 633
FspBI CTAG 3 cut(s) 194, 693, 852
GlaI GCGC 1 cut(s) 867
GluI GCNGC 2 cut(s) 630, 633
GsuI CTGGAG 2 cut(s) 200, 513
HaeIII GGCC 2 cut(s) 189, 883
HapII CCGG 3 cut(s) 932, 956, 965
HhaI GCGC 1 cut(s) 868
Hin1II CATG 3 cut(s) 60, 608, 703
Hin6I GCGC 1 cut(s) 866
HinP1I GCGC 1 cut(s) 866
HinfI GANTC 8 cut(s) 41, 125, 260, 350, 401, 655, 686, 876
HpaII CCGG 3 cut(s) 932, 956, 965
HphI GGTGA 1 cut(s) 946
Hpy166II GTNNAC 2 cut(s) 436, 953
Hpy188I TCNGA 4 cut(s) 23, 130, 355, 769
Hpy188III TCNNGA 7 cut(s) 416, 492, 590, 641, 663, 753, 896
Hpy8I GTNNAC 2 cut(s) 436, 953
Hpy99I CGWCG 2 cut(s) 443, 446
HpyAV CCTTC 3 cut(s) 46, 81, 602
HpyCH4III ACNGT 3 cut(s) 11, 458, 568
HpyCH4IV ACGT 3 cut(s) 235, 432, 577
HpyCH4V TGCA 4 cut(s) 4, 632, 745, 873
HpyF10VI GCNNNNNNNGC 1 cut(s) 800
HpyF3I CTNAG 5 cut(s) 217, 276, 282, 334, 1011
HpySE526I ACGT 3 cut(s) 235, 432, 577
Hsp92II CATG 3 cut(s) 60, 608, 703
HspAI GCGC 1 cut(s) 866
KroI GCCGGC 1 cut(s) 964
KroNI GCCGGC 1 cut(s) 966
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 5 cut(s) 409, 547, 611, 619, 941
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 3 cut(s) 138, 304, 601
Lsp1109I GCAGC 2 cut(s) 616, 644
LweI GCATC 1 cut(s) 838
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 3 cut(s) 194, 693, 852
MaeII ACGT 3 cut(s) 235, 432, 577
MaeIII GTNAC 4 cut(s) 95, 170, 695, 934
MalI GATC 5 cut(s) 411, 549, 613, 621, 943
MboI GATC 5 cut(s) 409, 547, 611, 619, 941
MboII GAAGA 9 cut(s) 239, 242, 311, 377, 410, 541, 608, 935, 971
MfeI CAATTG 1 cut(s) 885
MhlI GDGCHC 2 cut(s) 116, 135
MluCI AATT 9 cut(s) 197, 315, 380, 472, 507, 551, 746, 885, 991
MlyI GAGTC 4 cut(s) 35, 269, 344, 395
MmeI TCCRAC 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 964
MseI TTAA 1 cut(s) 318
MspI CCGG 3 cut(s) 932, 956, 965
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 885
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 1 cut(s) 800
NaeI GCCGGC 1 cut(s) 966
NdeII GATC 5 cut(s) 409, 547, 611, 619, 941
NgoMIV GCCGGC 1 cut(s) 964
NlaIII CATG 3 cut(s) 60, 608, 703
NlaIV GGNNCC 2 cut(s) 62, 468
NmeAIII GCCGAG 1 cut(s) 808
NmuCI GTSAC 2 cut(s) 695, 934
PciSI GCTCTTC 1 cut(s) 954
PcsI WCGNNNNNNNCGW 1 cut(s) 438
PdiI GCCGGC 1 cut(s) 966
PfeI GAWTC 4 cut(s) 125, 655, 686, 876
PinAI ACCGGT 1 cut(s) 931
PkrI GCNGC 2 cut(s) 631, 634
PleI GAGTC 4 cut(s) 35, 268, 344, 395
PpsI GAGTC 4 cut(s) 35, 268, 344, 395
Ppu21I YACGTR 1 cut(s) 433
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 2 cut(s) 62, 468
PspPI GGNCC 2 cut(s) 60, 953
PstI CTGCAG 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 902
RsaI GTAC 4 cut(s) 222, 431, 828, 999
RsaNI GTAC 4 cut(s) 221, 430, 827, 998
SacI GAGCTC 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 2 cut(s) 630, 633
Sau3AI GATC 5 cut(s) 409, 547, 611, 619, 941
Sau96I GGNCC 2 cut(s) 60, 953
SchI GAGTC 4 cut(s) 35, 269, 344, 395
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 2 cut(s) 116, 135
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 1 cut(s) 838
SfcI CTRYAG 3 cut(s) 405, 564, 630
SinI GGWCC 2 cut(s) 60, 953
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SnaBI TACGTA 1 cut(s) 433
Sse9I AATT 9 cut(s) 197, 315, 380, 472, 507, 551, 746, 885, 991
SsiI CCGC 2 cut(s) 972, 987
SspMI CTAG 3 cut(s) 194, 693, 852
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 11, 458, 568
TaiI ACGT 3 cut(s) 238, 435, 580
TaqI TCGA 8 cut(s) 39, 81, 258, 348, 583, 589, 614, 752
TaqII GACCGA 1 cut(s) 236
TasI AATT 9 cut(s) 197, 315, 380, 472, 507, 551, 746, 885, 991
TatI WGTACW 1 cut(s) 220
TfiI GAWTC 4 cut(s) 125, 655, 686, 876
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 4 cut(s) 314, 463, 907, 955
TseFI GTSAC 2 cut(s) 695, 934
TseI GCWGC 2 cut(s) 629, 632
Tsp45I GTSAC 2 cut(s) 695, 934
TspDTI ATGAA 7 cut(s) 45, 312, 437, 621, 678, 776, 942
TspGWI ACGGA 3 cut(s) 173, 667, 728
TspRI CASTG 4 cut(s) 314, 463, 907, 955
VpaK11BI GGWCC 2 cut(s) 60, 953
XapI RAATTY 1 cut(s) 197
XspI CTAG 3 cut(s) 194, 693, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.