Rorug02G0595200

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0006057
Physical Location & Seq
Reverse (-)
71672862 .. 71675109
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0595200.1

Sequence Viewer

Length: 870 bp
ATGGGTCAAGCACTTGGTTGTATTCTAGTGAAGGAGTCGACAGTTGTTGTGAAGGAACATTTTGGGAAGTTTGATGATGTGCTTGAACCAGGGTGTCACTGCTTGCCTCGGTGTTTCGGATACAAGGCAGCCGGTGTGCTATCAATGCGTGTGCGGCAATTCCAAGTTAAATGTGAAACTAAGACTAAGGATAATGTGTTTGTCAATGTGGTTGCATCGATTCAATTCCGTGCCTTACCAGAGAAAGTAGGGGATGCTTTCTACAAGCTCACTAACACCACTGCTCAGATTCAGGCCTATGTTTTCGATGTTATCAGGGCAAGTGTACCCAAATTGGAGTTGGATGCTGTGTTTGAACAGAAGAGTGACATAGCAAAAGCTGTTGAAGAGGAGCTTGAAAAGGCAATGTCTGCTTATGGTTTTGAAATCGTCGAGACTCTCATTGTTGATATTGTGCCTGATGATCGTGTGAAGAAAGCAATGAATGGGATCAATGCAGCTGCAAGACAGAGGGTTGCAGCAAAGGAAAAGGCTGAAGCCGAGAAGATACTACAGGTCAAGCGAGCTGAAGGAGAGGCAGAGTCCAAGTACTTGGCAGGGCTTGGGATAGCTCGCCAGCGCCAGGCCATTGTTGACGGGTTGAGGGACAGTGTTCTCGTCTTCTCTGAGAATGTTCCTGGAACTTCTCCCAAGGATGTCATGGACATGGTGCTGGTGACTCAATACTTCGACACTATGAAGGAGATAGGAGCATCCTCAAAGTCAACATCTGTCTTCATCCCACACGGGCCAGGTGCTGTCAAGGACGTTGCTTCCCAGATTCGAGAGGGTCTCCTCCAAGCTGATTCTGCTCACAATTTGAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

289

Amino Acids

31.6

Weight (kDa)

6.47

Isoelectric Point (pI)

25.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 10 - 182 2.7e-26 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 38
AciI CCGC 1 cut(s) 154
AclWI GGATC 1 cut(s) 497
AcuI CTGAAG 2 cut(s) 555, 588
AfaI GTAC 2 cut(s) 327, 590
AfiI CCNNNNNNNGG 1 cut(s) 622
AgsI TTSAA 7 cut(s) 86, 224, 356, 386, 398, 425, 862
AjnI CCWGG 4 cut(s) 88, 621, 676, 790
AluBI AGCT 7 cut(s) 268, 380, 394, 500, 566, 611, 842
AluI AGCT 7 cut(s) 268, 380, 394, 500, 566, 611, 842
Alw26I GTCTC 2 cut(s) 428, 836
AlwI GGATC 1 cut(s) 497
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 3 cut(s) 294, 624, 788
ApeKI GCWGC 4 cut(s) 128, 497, 500, 518
AspLEI GCGC 1 cut(s) 621
AspS9I GGNCC 1 cut(s) 788
AsuHPI GGTGA 1 cut(s) 727
BbsI GAAGAC 2 cut(s) 652, 766
BbvI GCAGC 4 cut(s) 140, 487, 509, 530
BciT130I CCWGG 4 cut(s) 90, 623, 678, 792
BciVI GTATCC 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 428, 836
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 551
BfoI RGCGCY 1 cut(s) 622
BfuI GTATCC 1 cut(s) 113
BisI GCNGC 5 cut(s) 129, 155, 498, 501, 519
BlsI GCNGC 5 cut(s) 130, 156, 499, 502, 520
BmcAI AGTACT 1 cut(s) 590
Bme1390I CCNGG 4 cut(s) 90, 623, 678, 792
BmgT120I GGNCC 1 cut(s) 788
BmrFI CCNGG 4 cut(s) 90, 623, 678, 792
BmsI GCATC 4 cut(s) 224, 244, 334, 761
BpiI GAAGAC 2 cut(s) 652, 766
BplI GAGNNNNNCTC 2 cut(s) 816, 848
Bsa29I ATCGAT 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 836
BsaJI CCNNGG 3 cut(s) 89, 107, 690
Bsc4I CCNNNNNNNGG 1 cut(s) 622
Bse118I RCCGGY 1 cut(s) 131
Bse3DI GCAATG 2 cut(s) 411, 486
BseBI CCWGG 4 cut(s) 90, 623, 678, 792
BseCI ATCGAT 1 cut(s) 218
BseDI CCNNGG 3 cut(s) 89, 107, 690
BseGI GGATG 5 cut(s) 259, 349, 700, 752, 777
BseLI CCNNNNNNNGG 1 cut(s) 622
BseMI GCAATG 2 cut(s) 411, 486
BseMII CTCAG 2 cut(s) 299, 657
BseRI GAGGAG 2 cut(s) 404, 824
BseXI GCAGC 4 cut(s) 140, 487, 509, 530
BshFI GGCC 3 cut(s) 296, 626, 790
BshVI ATCGAT 1 cut(s) 218
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 1 cut(s) 659
BslI CCNNNNNNNGG 1 cut(s) 622
BsmAI GTCTC 2 cut(s) 428, 836
BsmFI GGGAC 1 cut(s) 659
BsnI GGCC 3 cut(s) 296, 626, 790
Bso31I GGTCTC 1 cut(s) 836
Bsp143I GATC 2 cut(s) 463, 489
BspACI CCGC 1 cut(s) 154
BspANI GGCC 3 cut(s) 296, 626, 790
BspCNI CTCAG 2 cut(s) 298, 658
BspDI ATCGAT 1 cut(s) 218
BspPI GGATC 1 cut(s) 497
BspTNI GGTCTC 1 cut(s) 836
BsrDI GCAATG 2 cut(s) 411, 486
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 89, 107, 690
BssMI GATC 2 cut(s) 463, 489
BssT1I CCWWGG 1 cut(s) 690
Bst2UI CCWGG 4 cut(s) 90, 623, 678, 792
Bst4CI ACNGT 2 cut(s) 43, 650
Bst6I CTCTTC 2 cut(s) 356, 381
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 4 cut(s) 104, 564, 613, 617
BstDEI CTNAG 4 cut(s) 180, 186, 285, 666
BstF5I GGATG 5 cut(s) 259, 349, 700, 752, 777
BstH2I RGCGCY 1 cut(s) 622
BstHHI GCGC 1 cut(s) 621
BstKTI GATC 2 cut(s) 466, 492
BstMAI GTCTC 2 cut(s) 428, 836
BstMBI GATC 2 cut(s) 463, 489
BstMWI GCNNNNNNNGC 4 cut(s) 145, 154, 410, 848
BstNI CCWGG 4 cut(s) 90, 623, 678, 792
BstSCI CCNGG 4 cut(s) 88, 621, 676, 790
BstSFI CTRYAG 1 cut(s) 551
BstV1I GCAGC 4 cut(s) 140, 487, 509, 530
BstV2I GAAGAC 2 cut(s) 652, 766
BstXI CCANNNNNNTGG 1 cut(s) 592
Bsu15I ATCGAT 1 cut(s) 218
BsuI GTATCC 1 cut(s) 113
BsuRI GGCC 3 cut(s) 296, 626, 790
BsuTUI ATCGAT 1 cut(s) 218
BtsCI GGATG 5 cut(s) 259, 349, 700, 752, 777
BtsI GCAGTG 2 cut(s) 97, 279
BtsIMutI CAGTG 3 cut(s) 97, 279, 655
Cac8I GCNNGC 4 cut(s) 104, 564, 613, 617
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 1 cut(s) 621
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 1 cut(s) 788
ClaI ATCGAT 1 cut(s) 218
Csp6I GTAC 2 cut(s) 326, 589
CviAII CATG 2 cut(s) 700, 706
CviQI GTAC 2 cut(s) 326, 589
DdeI CTNAG 4 cut(s) 180, 186, 285, 666
DpnI GATC 2 cut(s) 465, 491
DpnII GATC 2 cut(s) 463, 489
Eam1104I CTCTTC 2 cut(s) 356, 381
EarI CTCTTC 2 cut(s) 356, 381
Eco130I CCWWGG 1 cut(s) 690
Eco147I AGGCCT 1 cut(s) 296
Eco31I GGTCTC 1 cut(s) 836
Eco57I CTGAAG 2 cut(s) 555, 588
EcoRII CCWGG 4 cut(s) 88, 621, 676, 790
EcoT14I CCWWGG 1 cut(s) 690
ErhI CCWWGG 1 cut(s) 690
FaeI CATG 2 cut(s) 703, 709
FaiI YATR 7 cut(s) 300, 371, 417, 701, 707, 737, 868
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 1 cut(s) 659
FatI CATG 2 cut(s) 699, 705
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 5 cut(s) 129, 155, 498, 501, 519
FokI GGATG 5 cut(s) 266, 356, 707, 739, 764
Fsp4HI GCNGC 5 cut(s) 129, 155, 498, 501, 519
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 620
GluI GCNGC 5 cut(s) 129, 155, 498, 501, 519
HaeII RGCGCY 1 cut(s) 622
HaeIII GGCC 3 cut(s) 296, 626, 790
HapII CCGG 1 cut(s) 132
HhaI GCGC 1 cut(s) 621
Hin1II CATG 2 cut(s) 703, 709
Hin6I GCGC 1 cut(s) 619
HinP1I GCGC 1 cut(s) 619
HincII GTYRAC 3 cut(s) 39, 634, 765
HindII GTYRAC 3 cut(s) 39, 634, 765
HinfI GANTC 8 cut(s) 35, 220, 289, 436, 581, 718, 820, 845
HpaII CCGG 1 cut(s) 132
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 4 cut(s) 39, 326, 634, 765
Hpy188I TCNGA 3 cut(s) 119, 288, 667
Hpy188III TCNNGA 2 cut(s) 433, 824
Hpy8I GTNNAC 4 cut(s) 39, 326, 634, 765
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 5 cut(s) 25, 46, 563, 733, 856
HpyCH4III ACNGT 2 cut(s) 43, 650
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 4 cut(s) 215, 497, 503, 518
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 154, 410, 848
HpyF3I CTNAG 4 cut(s) 180, 186, 285, 666
HpySE526I ACGT 1 cut(s) 807
Hsp92II CATG 2 cut(s) 703, 709
HspAI GCGC 1 cut(s) 619
Kzo9I GATC 2 cut(s) 463, 489
LmnI GCTCC 2 cut(s) 391, 749
Lsp1109I GCAGC 4 cut(s) 140, 487, 509, 530
LweI GCATC 4 cut(s) 224, 244, 334, 761
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 3 cut(s) 95, 365, 715
MalI GATC 2 cut(s) 465, 491
MboI GATC 2 cut(s) 463, 489
MboII GAAGA 6 cut(s) 373, 398, 484, 556, 652, 766
MluCI AATT 4 cut(s) 158, 224, 332, 856
MlyI GAGTC 4 cut(s) 44, 430, 590, 712
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 8 cut(s) 117, 382, 504, 568, 636, 766, 820, 845
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 4 cut(s) 90, 623, 678, 792
MvaI CCWGG 4 cut(s) 90, 623, 678, 792
MwoI GCNNNNNNNGC 4 cut(s) 145, 154, 410, 848
NdeII GATC 2 cut(s) 463, 489
NlaIII CATG 2 cut(s) 703, 709
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 3 cut(s) 95, 365, 715
PceI AGGCCT 1 cut(s) 296
PfeI GAWTC 4 cut(s) 220, 289, 820, 845
PfoI TCCNGGA 1 cut(s) 676
PkrI GCNGC 5 cut(s) 130, 156, 499, 502, 520
PleI GAGTC 4 cut(s) 43, 430, 589, 712
PpsI GAGTC 4 cut(s) 43, 430, 589, 712
Psp6I CCWGG 4 cut(s) 88, 621, 676, 790
PspGI CCWGG 4 cut(s) 88, 621, 676, 790
PspPI GGNCC 1 cut(s) 788
PstNI CAGNNNCTG 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 327, 590
RsaNI GTAC 2 cut(s) 326, 589
RseI CAYNNNNRTG 1 cut(s) 704
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 5 cut(s) 129, 155, 498, 501, 519
Sau3AI GATC 2 cut(s) 463, 489
Sau96I GGNCC 1 cut(s) 788
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 4 cut(s) 44, 430, 590, 712
ScrFI CCNGG 4 cut(s) 90, 623, 678, 792
SfaNI GCATC 4 cut(s) 224, 244, 334, 761
SfcI CTRYAG 1 cut(s) 551
SmiMI CAYNNNNRTG 1 cut(s) 704
Sse9I AATT 4 cut(s) 158, 224, 332, 856
SseBI AGGCCT 1 cut(s) 296
SsiI CCGC 1 cut(s) 154
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 1 cut(s) 296
StyD4I CCNGG 4 cut(s) 88, 621, 676, 790
StyI CCWWGG 1 cut(s) 690
TaaI ACNGT 2 cut(s) 43, 650
TaiI ACGT 1 cut(s) 810
TaqI TCGA 6 cut(s) 38, 218, 306, 432, 729, 823
TasI AATT 4 cut(s) 158, 224, 332, 856
TatI WGTACW 1 cut(s) 588
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 4 cut(s) 220, 289, 820, 845
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 3 cut(s) 104, 286, 655
TseFI GTSAC 3 cut(s) 95, 365, 715
TseI GCWGC 4 cut(s) 128, 497, 500, 518
Tsp45I GTSAC 3 cut(s) 95, 365, 715
TspDTI ATGAA 3 cut(s) 497, 752, 766
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 3 cut(s) 104, 286, 655
XcmI CCANNNNNNNNNTGG 2 cut(s) 337, 697
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 1 cut(s) 26
ZrmI AGTACT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.