Rw2G055240

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006057
Physical Location & Seq
Forward (+)
82698569 .. 82700203
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G055240.1

Sequence Viewer

Length: 1269 bp
ATGGAAGAGAGTTTCAGCGTTCGAGTAAGCAAAACGTTCGATATTCTAGATGGCTCATCACCATCAGCGACAGCGAGTGCTCCGTCTTCGTCGCTTTGGTCCCTAACAGGTGAGGAAATCGAGAAGAGAGAATGGAACAGGGACAAGGGGAGCCCCGAACCCCAACCCCGACCCTGCAACCCAGATTCAAATTCAAAGGACGATTTCTTCGGTAACGAGCTGGAGAAGGACCTTCTGGACCTTGACGACGACGACGACGACGACGACGTGGAAGAAGAAGAAGAAGAAGACGAGGTGGTGGAGGAGCAATCTGCTAAGCCTAAGCCGAAGCCGAAGCCTATGCCGGATGATTATAACGAGGAGCAGTGGCAAATTAAAACCTGCATTGGCTTGGACTGTACTCTCGACCACGAGGTAACCTTGCTCTCTCATTCTAACAGAAATGCTGCAGACCGCTTTTATATGAAGGATGTAAATGACTATCCGATAGACATTGATTCTCAATACGAGGTTCCCAATTCATTGAACGATTTCACTAGAGACCCTCGCGCCAATCACCTAGCAGCTAAGATTAGACTCCAACTGGATGCAGAAGCAGCTCGAAAAATTGACTCCTTGCGGGTCTCTGGGAACACTGCACCATCCGGTGCTATAGCTGATGAATTCGTCCCGTCTGAAGATGCTATCAACCTGAAATCAATTTTGAAGAGGAAAAATGATGAGCAGTTAGGCTCATCTAAATCACAAAAGCGTGTTCGGTTTGACCCTGAGTGTAAAGATGATTGCGATCAAGAGCCTGATGGATCCAAAGATATACCTGTGAAAAGCCTTTCAGGTGAAGATCATGTGTCTGCAGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATACATTTGATTCATCAACTGACATGGATGAGGAAGCTAACAGGCAAGCCTATATGGATTTCCGTAATCTTTTGGGGATGTCAAATACTACGGATGCAGAAGTTTCAAAACCGATCATATTCACACCAAAAAAGAAATCAACTGATGCGACAATGCTAGAAATTGATGGGGAGTCGGAGAGACCAGTAGGTGCTAGTAAGGAATCTGCGTCTTACAGAGTCGTGCCAATTGCTATTGCAGCCAGGGATCACGAAGACAGTGATGTGTGTGCAATGGATGAAGATGAACCAGATATAGAAACCAGTGGAAGAACAAGTTCACAGAGGATTGGCCGCCAGTTTCGTACAAGAACCAGTTTAGAGTTGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

422

Amino Acids

47.44

Weight (kDa)

4.41

Isoelectric Point (pI)

54.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 277 - 329 1.6e-06 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 389
AccII CGCG 1 cut(s) 549
AciI CCGC 3 cut(s) 454, 619, 1228
AclI AACGTT 1 cut(s) 35
AclWI GGATC 3 cut(s) 798, 811, 1149
AcoI YGGCCR 1 cut(s) 1225
AcsI RAATTY 2 cut(s) 190, 662
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 2 cut(s) 400, 1240
AgsI TTSAA 5 cut(s) 189, 195, 526, 706, 1002
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 1 cut(s) 1136
AluBI AGCT 5 cut(s) 220, 566, 599, 656, 932
AluI AGCT 5 cut(s) 220, 566, 599, 656, 932
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 3 cut(s) 534, 628, 1069
AlwI GGATC 3 cut(s) 798, 811, 1149
AoxI GGCC 1 cut(s) 1225
ApeKI GCWGC 4 cut(s) 446, 563, 596, 1133
ApoI RAATTY 2 cut(s) 190, 662
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
Asp700I GAANNNNTTC 2 cut(s) 530, 1210
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 3 cut(s) 99, 229, 238
AsuHPI GGTGA 4 cut(s) 51, 122, 548, 848
AvaII GGWCC 3 cut(s) 99, 229, 238
BamHI GGATCC 1 cut(s) 803
BanII GRGCYC 1 cut(s) 155
BauI CACGAG 1 cut(s) 410
BbsI GAAGAC 3 cut(s) 78, 294, 1155
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 4 cut(s) 433, 575, 608, 1145
BccI CCATC 6 cut(s) 44, 70, 649, 794, 886, 1055
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 322, 356
BciT130I CCWGG 1 cut(s) 1138
BcoDI GTCTC 3 cut(s) 534, 628, 1069
BfaI CTAG 5 cut(s) 47, 537, 560, 1052, 1089
BfmI CTRYAG 3 cut(s) 447, 651, 852
BfuAI ACCTGC 1 cut(s) 389
BisI GCNGC 5 cut(s) 447, 564, 597, 1134, 1228
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 5 cut(s) 448, 565, 598, 1135, 1229
Bme1390I CCNGG 1 cut(s) 1138
Bme18I GGWCC 3 cut(s) 99, 229, 238
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 3 cut(s) 99, 229, 238
BmiI GGNNCC 4 cut(s) 101, 152, 513, 805
BmrFI CCNGG 1 cut(s) 1138
BmsI GCATC 4 cut(s) 577, 670, 979, 1030
BpiI GAAGAC 3 cut(s) 78, 294, 1155
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 315
BsaBI GATNNNNATC 1 cut(s) 786
BsaI GGTCTC 3 cut(s) 534, 628, 1069
BsaJI CCNNGG 1 cut(s) 1137
BsaWI WCCGGW 1 cut(s) 644
Bse1I ACTGG 5 cut(s) 588, 1079, 1197, 1231, 1248
Bse3DI GCAATG 1 cut(s) 1173
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 1 cut(s) 1138
BseDI CCNNGG 1 cut(s) 1137
BseGI GGATG 9 cut(s) 352, 475, 592, 641, 928, 978, 994, 1177, 1267
BseJI GATNNNNATC 1 cut(s) 786
BseMI GCAATG 1 cut(s) 1173
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 5 cut(s) 588, 1079, 1197, 1231, 1248
BseRI GAGGAG 2 cut(s) 317, 374
BseXI GCAGC 4 cut(s) 433, 575, 608, 1145
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 1227
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 2 cut(s) 344, 645
BslFI GGGAC 3 cut(s) 85, 155, 653
BsmAI GTCTC 3 cut(s) 534, 628, 1069
BsmFI GGGAC 3 cut(s) 85, 155, 653
BsnI GGCC 1 cut(s) 1227
Bso31I GGTCTC 3 cut(s) 534, 628, 1069
Bsp1286I GDGCHC 2 cut(s) 82, 155
Bsp143I GATC 5 cut(s) 787, 803, 841, 1008, 1141
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 3 cut(s) 454, 619, 1228
BspANI GGCC 1 cut(s) 1227
BspCNI CTCAG 1 cut(s) 760
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 4 cut(s) 101, 152, 513, 805
BspMAI CTGCAG 2 cut(s) 451, 856
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 3 cut(s) 798, 811, 1149
BspTNI GGTCTC 3 cut(s) 534, 628, 1069
BsrDI GCAATG 1 cut(s) 1173
BsrI ACTGG 5 cut(s) 588, 1079, 1197, 1231, 1248
BssECI CCNNGG 1 cut(s) 1137
BssMI GATC 5 cut(s) 787, 803, 841, 1008, 1141
BssSI CACGAG 1 cut(s) 410
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 1 cut(s) 1138
Bst4CI ACNGT 2 cut(s) 398, 1154
Bst6I CTCTTC 2 cut(s) 119, 701
BstC8I GCNNGC 1 cut(s) 942
BstDEI CTNAG 4 cut(s) 315, 321, 567, 768
BstEII GGTNACC 1 cut(s) 415
BstF5I GGATG 9 cut(s) 352, 475, 592, 641, 928, 978, 994, 1177, 1267
BstFNI CGCG 1 cut(s) 549
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 5 cut(s) 790, 806, 844, 1011, 1144
BstMAI GTCTC 3 cut(s) 534, 628, 1069
BstMBI GATC 5 cut(s) 787, 803, 841, 1008, 1141
BstMWI GCNNNNNNNGC 2 cut(s) 596, 1133
BstNI CCWGG 1 cut(s) 1138
BstPI GGTNACC 1 cut(s) 415
BstSCI CCNGG 1 cut(s) 1136
BstSFI CTRYAG 3 cut(s) 447, 651, 852
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 4 cut(s) 433, 575, 608, 1145
BstV2I GAAGAC 3 cut(s) 78, 294, 1155
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 1 cut(s) 1227
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 9 cut(s) 352, 475, 592, 641, 928, 978, 994, 1177, 1267
BtsI GCAGTG 2 cut(s) 371, 633
BtsIMutI CAGTG 4 cut(s) 371, 633, 1159, 1204
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 1 cut(s) 942
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 3 cut(s) 99, 229, 238
CseI GACGC 1 cut(s) 1092
Csp6I GTAC 2 cut(s) 399, 1239
CviAII CATG 2 cut(s) 845, 919
CviQI GTAC 2 cut(s) 399, 1239
DdeI CTNAG 4 cut(s) 315, 321, 567, 768
DpnI GATC 5 cut(s) 789, 805, 843, 1010, 1143
DpnII GATC 5 cut(s) 787, 803, 841, 1008, 1141
EaeI YGGCCR 1 cut(s) 1225
Eam1104I CTCTTC 2 cut(s) 119, 701
EarI CTCTTC 2 cut(s) 119, 701
Eco24I GRGCYC 1 cut(s) 155
Eco31I GGTCTC 3 cut(s) 534, 628, 1069
Eco47I GGWCC 3 cut(s) 99, 229, 238
Eco57I CTGAAG 1 cut(s) 696
Eco91I GGTNACC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 1136
EcoT38I GRGCYC 1 cut(s) 155
FaeI CATG 2 cut(s) 848, 922
FaqI GGGAC 3 cut(s) 85, 155, 653
FatI CATG 2 cut(s) 844, 918
FauI CCCGC 1 cut(s) 612
Fnu4HI GCNGC 5 cut(s) 447, 564, 597, 1134, 1228
FokI GGATG 8 cut(s) 359, 482, 599, 628, 935, 985, 1001, 1184
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 5 cut(s) 447, 564, 597, 1134, 1228
FspBI CTAG 5 cut(s) 47, 537, 560, 1052, 1089
GlaI GCGC 1 cut(s) 550
GluI GCNGC 5 cut(s) 447, 564, 597, 1134, 1228
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 1 cut(s) 1227
HapII CCGG 2 cut(s) 344, 645
HgaI GACGC 1 cut(s) 1092
HhaI GCGC 1 cut(s) 551
Hin1II CATG 2 cut(s) 848, 922
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HinfI GANTC 9 cut(s) 185, 497, 576, 611, 874, 905, 1067, 1097, 1113
HpaII CCGG 2 cut(s) 344, 645
HphI GGTGA 4 cut(s) 51, 122, 548, 848
Hpy166II GTNNAC 1 cut(s) 1214
Hpy188I TCNGA 3 cut(s) 486, 676, 1072
Hpy188III TCNNGA 8 cut(s) 47, 121, 236, 404, 791, 860, 882, 1145
Hpy8I GTNNAC 1 cut(s) 1214
Hpy99I CGWCG 8 cut(s) 94, 251, 254, 257, 260, 263, 266, 269
HpyAV CCTTC 3 cut(s) 220, 242, 460
HpyCH4III ACNGT 2 cut(s) 398, 1154
HpyCH4IV ACGT 2 cut(s) 35, 267
HpyCH4V TGCA 9 cut(s) 177, 384, 449, 590, 638, 854, 992, 1133, 1166
HpyF10VI GCNNNNNNNGC 2 cut(s) 596, 1133
HpyF3I CTNAG 4 cut(s) 315, 321, 567, 768
HpySE526I ACGT 2 cut(s) 35, 267
Hsp92II CATG 2 cut(s) 848, 922
HspAI GCGC 1 cut(s) 549
Kzo9I GATC 5 cut(s) 787, 803, 841, 1008, 1141
LmnI GCTCC 4 cut(s) 85, 150, 304, 361
Lsp1109I GCAGC 4 cut(s) 433, 575, 608, 1145
LweI GCATC 4 cut(s) 577, 670, 979, 1030
MaeI CTAG 5 cut(s) 47, 537, 560, 1052, 1089
MaeII ACGT 2 cut(s) 35, 267
MaeIII GTNAC 2 cut(s) 212, 415
MalI GATC 5 cut(s) 789, 805, 843, 1010, 1143
MboI GATC 5 cut(s) 787, 803, 841, 1008, 1141
MfeI CAATTG 1 cut(s) 1122
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 2 cut(s) 82, 155
MluCI AATT 8 cut(s) 190, 372, 517, 606, 662, 699, 1056, 1122
MlyI GAGTC 4 cut(s) 570, 605, 1076, 1122
MmeI TCCRAC 3 cut(s) 604, 1050, 1239
MroXI GAANNNNTTC 2 cut(s) 530, 1210
MseI TTAA 1 cut(s) 375
MspI CCGG 2 cut(s) 344, 645
MspR9I CCNGG 1 cut(s) 1138
MunI CAATTG 1 cut(s) 1122
MvaI CCWGG 1 cut(s) 1138
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 2 cut(s) 596, 1133
NdeII GATC 5 cut(s) 787, 803, 841, 1008, 1141
NlaIII CATG 2 cut(s) 848, 922
NlaIV GGNNCC 4 cut(s) 101, 152, 513, 805
PcsI WCGNNNNNNNCGW 5 cut(s) 252, 255, 258, 261, 264
PdmI GAANNNNTTC 2 cut(s) 530, 1210
PfeI GAWTC 5 cut(s) 185, 497, 874, 905, 1097
PkrI GCNGC 5 cut(s) 448, 565, 598, 1135, 1229
PleI GAGTC 4 cut(s) 570, 605, 1075, 1121
PpsI GAGTC 4 cut(s) 570, 605, 1075, 1121
PpuMI RGGWCCY 1 cut(s) 229
PsiI TTATAA 1 cut(s) 354
Psp1406I AACGTT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 1136
PspEI GGTNACC 1 cut(s) 415
PspGI CCWGG 1 cut(s) 1136
PspN4I GGNNCC 4 cut(s) 101, 152, 513, 805
PspPI GGNCC 3 cut(s) 99, 229, 238
PspPPI RGGWCCY 1 cut(s) 229
PstI CTGCAG 2 cut(s) 451, 856
PsuI RGATCY 1 cut(s) 803
RsaI GTAC 2 cut(s) 400, 1240
RsaNI GTAC 2 cut(s) 399, 1239
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 5 cut(s) 447, 564, 597, 1134, 1228
Sau3AI GATC 5 cut(s) 787, 803, 841, 1008, 1141
Sau96I GGNCC 3 cut(s) 99, 229, 238
SchI GAGTC 4 cut(s) 570, 605, 1076, 1122
ScrFI CCNGG 1 cut(s) 1138
SduI GDGCHC 2 cut(s) 82, 155
SfaNI GCATC 4 cut(s) 577, 670, 979, 1030
SfcI CTRYAG 3 cut(s) 447, 651, 852
SinI GGWCC 3 cut(s) 99, 229, 238
Sse9I AATT 8 cut(s) 190, 372, 517, 606, 662, 699, 1056, 1122
SsiI CCGC 3 cut(s) 454, 619, 1228
SspMI CTAG 5 cut(s) 47, 537, 560, 1052, 1089
StyD4I CCNGG 1 cut(s) 1136
TaaI ACNGT 2 cut(s) 398, 1154
TaiI ACGT 2 cut(s) 38, 270
TaqI TCGA 5 cut(s) 22, 39, 120, 405, 601
TasI AATT 8 cut(s) 190, 372, 517, 606, 662, 699, 1056, 1122
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 1230
TfiI GAWTC 5 cut(s) 185, 497, 874, 905, 1097
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 4 cut(s) 371, 640, 1159, 1204
TseI GCWGC 4 cut(s) 446, 563, 596, 1133
TspDTI ATGAA 6 cut(s) 479, 510, 675, 897, 1188, 1194
TspGWI ACGGA 4 cut(s) 72, 886, 947, 1001
TspRI CASTG 4 cut(s) 371, 640, 1159, 1204
VpaK11BI GGWCC 3 cut(s) 99, 229, 238
XapI RAATTY 2 cut(s) 190, 662
XbaI TCTAGA 1 cut(s) 46
XcmI CCANNNNNNNNNTGG 1 cut(s) 1255
XmnI GAANNNNTTC 2 cut(s) 530, 1210
XspI CTAG 5 cut(s) 47, 537, 560, 1052, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.