Rroxscaffold_2G00076360

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006057
Physical Location & Seq
Reverse (-)
223967 .. 225777
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076360.1

Sequence Viewer

Length: 1272 bp
ATGGAAGAGAGTTTCAGCGTTCGAGTAGGCAAAACGTTCGATATTCTAGCTGGCTCATCACCATCAGCGACAGCGAGGGCTCCGTCTTCGTCGCTGTGGTCCCTAACAGGTGAGGAAATCGAGAAGAGAGAATGGAACAGGGACAAGGGGAGCCCCGAACCCCAACCCCGACCCTGCAACCCAAATTCAAAGGATGATTTCTTCGGTAACGAGCTGGAGAAGGACCTTCTGGACCTTGACGACGACGATGACGTGGAAGAAGAAGAAGAAGACGAGGTGGTGGAGGAGCAATCTGCTAAGCCTAAGCCGAAGCCTATGCCGGATGATTATAACGAGGAGCAGTGGCAAATTAAAACCTGCATTGGCTTGGACTGTACTCTCGACCACGAGGAAGAGGAAGATGAATTTGACAAAGTGGCTGTTGGCAAGGGAAATGCTGGAGACCGCTTTTATATGAAGGATGTAAATGACTATCCGATAGACATTGATTCTCAATACGAGGTTCCCAATTCATTGAACGATTTCACTAGAGACCCTCGCGCCAATCACCTAGCAGCGAAGATTAGACTCCAACAGGATGCAGAAGCAGCTCAAAAAATTGACTCCTTGCGGGTTTCTGGGAACACTGCACCATCCGGTGCTATAGCTGATGAATTCATCCCGTCTGAAGATGCTATCAACCTGAAATCAATTTTGAAGAGGAAAAATGATGAGCAGTTAGGCTCATCTAAATCACAAAAGCGTGTTCGGTTTGACCCTGAGTGTAAAGATGATTGCGATGAAGAGCCTGATGGATCCAAAGATATACCTGTGAAAAGCCCTTCAGGTGAAGATCATGTGTCTGCAGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATACATTTGATTCATCAACTGACATGGATGAGGAAGCTAACAGGCAAGCCTATATGGATTTCCGTAATCTTTTGGGGATGTCAAATACTACGGATGCAGAAGCACCCAGGGATTTTTCAAAACCGATCATATTCACACCAAAAAAGAAATCAACTGATGCGACAATGCTAGAAATTGATGGGGAGTCGGAGAGACCAGTAGGTGCTAGTAAGGAATCTGCGCCTTACAGAGTCGTGCCAATTGCTATTGCAGCCAGGGATCACGAAGACAGTGATGTGTGTGCAATGGATGAAGATGAACCAGATATAGAAACCAGTGGAAGAACAAGTTCACAGAGGATTGGCCGCCAGTTTCGTACAAGAACCAGTTTAGAGTTGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

423

Amino Acids

47.53

Weight (kDa)

4.4

Isoelectric Point (pI)

57.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 274 - 326 1.1e-06 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 330
Acc36I ACCTGC 1 cut(s) 365
AccII CGCG 1 cut(s) 540
AciI CCGC 3 cut(s) 445, 610, 1231
AclI AACGTT 1 cut(s) 35
AclWI GGATC 3 cut(s) 789, 802, 1152
AcoI YGGCCR 1 cut(s) 1228
AcsI RAATTY 3 cut(s) 184, 404, 653
AcuI CTGAAG 2 cut(s) 687, 807
AfaI GTAC 2 cut(s) 376, 1243
AgsI TTSAA 4 cut(s) 189, 517, 697, 1005
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 2 cut(s) 992, 1139
AluBI AGCT 5 cut(s) 50, 214, 590, 647, 923
AluI AGCT 5 cut(s) 50, 214, 590, 647, 923
Alw26I GTCTC 3 cut(s) 435, 525, 1072
AlwI GGATC 3 cut(s) 789, 802, 1152
AoxI GGCC 1 cut(s) 1228
ApeKI GCWGC 3 cut(s) 554, 587, 1136
ApoI RAATTY 3 cut(s) 184, 404, 653
Asp700I GAANNNNTTC 2 cut(s) 521, 1213
AspLEI GCGC 2 cut(s) 542, 1108
AspS9I GGNCC 3 cut(s) 99, 223, 232
AsuHPI GGTGA 4 cut(s) 51, 122, 539, 839
AvaII GGWCC 3 cut(s) 99, 223, 232
BamHI GGATCC 1 cut(s) 794
BanII GRGCYC 2 cut(s) 82, 155
BauI CACGAG 1 cut(s) 386
BbsI GAAGAC 3 cut(s) 78, 276, 1158
BbvI GCAGC 3 cut(s) 566, 599, 1148
BccI CCATC 5 cut(s) 70, 640, 785, 877, 1058
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 298, 332
BciT130I CCWGG 2 cut(s) 994, 1141
BcoDI GTCTC 3 cut(s) 435, 525, 1072
BfaI CTAG 5 cut(s) 47, 528, 551, 1055, 1092
BfmI CTRYAG 2 cut(s) 642, 843
BfuAI ACCTGC 1 cut(s) 365
BisI GCNGC 4 cut(s) 555, 588, 1137, 1231
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 4 cut(s) 556, 589, 1138, 1232
Bme1390I CCNGG 2 cut(s) 994, 1141
Bme18I GGWCC 3 cut(s) 99, 223, 232
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 99, 223, 232
BmiI GGNNCC 5 cut(s) 81, 101, 152, 504, 796
BmrFI CCNGG 2 cut(s) 994, 1141
BmsI GCATC 4 cut(s) 568, 661, 970, 1033
BpiI GAAGAC 3 cut(s) 78, 276, 1158
BpmI CTGGAG 2 cut(s) 236, 459
Bpu10I CCTNAGC 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 297
BsaI GGTCTC 3 cut(s) 435, 525, 1072
BsaJI CCNNGG 3 cut(s) 992, 993, 1140
BsaWI WCCGGW 1 cut(s) 635
Bse1I ACTGG 4 cut(s) 1082, 1200, 1234, 1251
Bse3DI GCAATG 1 cut(s) 1176
BseBI CCWGG 2 cut(s) 994, 1141
BseDI CCNNGG 3 cut(s) 992, 993, 1140
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 4 cut(s) 1082, 1200, 1234, 1251
BseRI GAGGAG 2 cut(s) 299, 350
BseXI GCAGC 3 cut(s) 566, 599, 1148
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 540
BshFI GGCC 1 cut(s) 1230
BsiSI CCGG 2 cut(s) 320, 636
BslFI GGGAC 2 cut(s) 85, 155
BsmAI GTCTC 3 cut(s) 435, 525, 1072
BsmFI GGGAC 2 cut(s) 85, 155
BsnI GGCC 1 cut(s) 1230
Bso31I GGTCTC 3 cut(s) 435, 525, 1072
Bsp1286I GDGCHC 2 cut(s) 82, 155
Bsp143I GATC 4 cut(s) 794, 832, 1011, 1144
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 3 cut(s) 445, 610, 1231
BspANI GGCC 1 cut(s) 1230
BspCNI CTCAG 1 cut(s) 751
BspFNI CGCG 1 cut(s) 540
BspLI GGNNCC 5 cut(s) 81, 101, 152, 504, 796
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 365
BspPI GGATC 3 cut(s) 789, 802, 1152
BspQI GCTCTTC 1 cut(s) 777
BspTNI GGTCTC 3 cut(s) 435, 525, 1072
BsrDI GCAATG 1 cut(s) 1176
BsrI ACTGG 4 cut(s) 1082, 1200, 1234, 1251
BssECI CCNNGG 3 cut(s) 992, 993, 1140
BssMI GATC 4 cut(s) 794, 832, 1011, 1144
BssSI CACGAG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 994, 1141
Bst4CI ACNGT 2 cut(s) 374, 1157
Bst6I CTCTTC 4 cut(s) 119, 387, 692, 777
BstC8I GCNNGC 2 cut(s) 52, 933
BstDEI CTNAG 3 cut(s) 297, 303, 759
BstFNI CGCG 1 cut(s) 540
BstHHI GCGC 2 cut(s) 542, 1108
BstKTI GATC 4 cut(s) 797, 835, 1014, 1147
BstMAI GTCTC 3 cut(s) 435, 525, 1072
BstMBI GATC 4 cut(s) 794, 832, 1011, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 587, 1136
BstNI CCWGG 2 cut(s) 994, 1141
BstSCI CCNGG 2 cut(s) 992, 1139
BstSFI CTRYAG 2 cut(s) 642, 843
BstUI CGCG 1 cut(s) 540
BstV1I GCAGC 3 cut(s) 566, 599, 1148
BstV2I GAAGAC 3 cut(s) 78, 276, 1158
BstX2I RGATCY 1 cut(s) 794
BstYI RGATCY 1 cut(s) 794
BsuRI GGCC 1 cut(s) 1230
BtgZI GCGATG 1 cut(s) 792
BtrI CACGTC 1 cut(s) 253
BtsI GCAGTG 2 cut(s) 347, 624
BtsIMutI CAGTG 4 cut(s) 347, 624, 1162, 1207
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 52, 933
CfoI GCGC 2 cut(s) 542, 1108
Cfr13I GGNCC 3 cut(s) 99, 223, 232
Csp6I GTAC 2 cut(s) 375, 1242
CviAII CATG 2 cut(s) 836, 910
CviQI GTAC 2 cut(s) 375, 1242
DdeI CTNAG 3 cut(s) 297, 303, 759
DpnI GATC 4 cut(s) 796, 834, 1013, 1146
DpnII GATC 4 cut(s) 794, 832, 1011, 1144
EaeI YGGCCR 1 cut(s) 1228
Eam1104I CTCTTC 4 cut(s) 119, 387, 692, 777
EarI CTCTTC 4 cut(s) 119, 387, 692, 777
Eco24I GRGCYC 2 cut(s) 82, 155
Eco31I GGTCTC 3 cut(s) 435, 525, 1072
Eco47I GGWCC 3 cut(s) 99, 223, 232
Eco57I CTGAAG 2 cut(s) 687, 807
EcoO109I RGGNCCY 1 cut(s) 223
EcoRI GAATTC 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 992, 1139
EcoT38I GRGCYC 2 cut(s) 82, 155
FaeI CATG 2 cut(s) 839, 913
FaqI GGGAC 2 cut(s) 85, 155
FatI CATG 2 cut(s) 835, 909
FauI CCCGC 1 cut(s) 603
Fnu4HI GCNGC 4 cut(s) 555, 588, 1137, 1231
FriOI GRGCYC 2 cut(s) 82, 155
Fsp4HI GCNGC 4 cut(s) 555, 588, 1137, 1231
FspBI CTAG 5 cut(s) 47, 528, 551, 1055, 1092
GlaI GCGC 2 cut(s) 541, 1107
GluI GCNGC 4 cut(s) 555, 588, 1137, 1231
GsuI CTGGAG 2 cut(s) 236, 459
HaeIII GGCC 1 cut(s) 1230
HapII CCGG 2 cut(s) 320, 636
HhaI GCGC 2 cut(s) 542, 1108
Hin1II CATG 2 cut(s) 839, 913
Hin6I GCGC 2 cut(s) 540, 1106
HinP1I GCGC 2 cut(s) 540, 1106
HinfI GANTC 8 cut(s) 488, 567, 602, 865, 896, 1070, 1100, 1116
HpaII CCGG 2 cut(s) 320, 636
HphI GGTGA 4 cut(s) 51, 122, 539, 839
Hpy166II GTNNAC 1 cut(s) 1217
Hpy188I TCNGA 3 cut(s) 477, 667, 1075
Hpy188III TCNNGA 6 cut(s) 121, 230, 380, 851, 873, 1148
Hpy8I GTNNAC 1 cut(s) 1217
Hpy99I CGWCG 3 cut(s) 94, 245, 248
HpyAV CCTTC 4 cut(s) 214, 236, 451, 831
HpyCH4III ACNGT 2 cut(s) 374, 1157
HpyCH4IV ACGT 2 cut(s) 35, 252
HpyCH4V TGCA 8 cut(s) 177, 360, 581, 629, 845, 983, 1136, 1169
HpyF10VI GCNNNNNNNGC 2 cut(s) 587, 1136
HpyF3I CTNAG 3 cut(s) 297, 303, 759
HpySE526I ACGT 2 cut(s) 35, 252
Hsp92II CATG 2 cut(s) 839, 913
HspAI GCGC 2 cut(s) 540, 1106
Kzo9I GATC 4 cut(s) 794, 832, 1011, 1144
LguI GCTCTTC 1 cut(s) 777
LmnI GCTCC 4 cut(s) 85, 150, 286, 337
Lsp1109I GCAGC 3 cut(s) 566, 599, 1148
LweI GCATC 4 cut(s) 568, 661, 970, 1033
MaeI CTAG 5 cut(s) 47, 528, 551, 1055, 1092
MaeII ACGT 2 cut(s) 35, 252
MaeIII GTNAC 1 cut(s) 206
MalI GATC 4 cut(s) 796, 834, 1013, 1146
MboI GATC 4 cut(s) 794, 832, 1011, 1144
MfeI CAATTG 1 cut(s) 1125
MflI RGATCY 1 cut(s) 794
MhlI GDGCHC 2 cut(s) 82, 155
MluCI AATT 9 cut(s) 184, 348, 404, 508, 597, 653, 690, 1059, 1125
MlyI GAGTC 4 cut(s) 561, 596, 1079, 1125
MmeI TCCRAC 3 cut(s) 595, 1053, 1242
MroXI GAANNNNTTC 2 cut(s) 521, 1213
MseI TTAA 1 cut(s) 351
MspI CCGG 2 cut(s) 320, 636
MspR9I CCNGG 2 cut(s) 994, 1141
MunI CAATTG 1 cut(s) 1125
MvaI CCWGG 2 cut(s) 994, 1141
MvnI CGCG 1 cut(s) 540
MwoI GCNNNNNNNGC 2 cut(s) 587, 1136
NdeII GATC 4 cut(s) 794, 832, 1011, 1144
NlaIII CATG 2 cut(s) 839, 913
NlaIV GGNNCC 5 cut(s) 81, 101, 152, 504, 796
PasI CCCWGGG 1 cut(s) 993
PciSI GCTCTTC 1 cut(s) 777
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdmI GAANNNNTTC 2 cut(s) 521, 1213
PfeI GAWTC 4 cut(s) 488, 865, 896, 1100
PkrI GCNGC 4 cut(s) 556, 589, 1138, 1232
PleI GAGTC 4 cut(s) 561, 596, 1078, 1124
PpsI GAGTC 4 cut(s) 561, 596, 1078, 1124
PpuMI RGGWCCY 1 cut(s) 223
PsiI TTATAA 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 992, 1139
PspGI CCWGG 2 cut(s) 992, 1139
PspN4I GGNNCC 5 cut(s) 81, 101, 152, 504, 796
PspPI GGNCC 3 cut(s) 99, 223, 232
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 1 cut(s) 794
RsaI GTAC 2 cut(s) 376, 1243
RsaNI GTAC 2 cut(s) 375, 1242
SapI GCTCTTC 1 cut(s) 777
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 4 cut(s) 555, 588, 1137, 1231
Sau3AI GATC 4 cut(s) 794, 832, 1011, 1144
Sau96I GGNCC 3 cut(s) 99, 223, 232
SchI GAGTC 4 cut(s) 561, 596, 1079, 1125
ScrFI CCNGG 2 cut(s) 994, 1141
SduI GDGCHC 2 cut(s) 82, 155
SfaNI GCATC 4 cut(s) 568, 661, 970, 1033
SfcI CTRYAG 2 cut(s) 642, 843
SinI GGWCC 3 cut(s) 99, 223, 232
Sse9I AATT 9 cut(s) 184, 348, 404, 508, 597, 653, 690, 1059, 1125
SsiI CCGC 3 cut(s) 445, 610, 1231
SspMI CTAG 5 cut(s) 47, 528, 551, 1055, 1092
StyD4I CCNGG 2 cut(s) 992, 1139
TaaI ACNGT 2 cut(s) 374, 1157
TaiI ACGT 2 cut(s) 38, 255
TaqI TCGA 4 cut(s) 22, 39, 120, 381
TasI AATT 9 cut(s) 184, 348, 404, 508, 597, 653, 690, 1059, 1125
TatI WGTACW 1 cut(s) 374
TauI GCSGC 1 cut(s) 1233
TfiI GAWTC 4 cut(s) 488, 865, 896, 1100
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 4 cut(s) 347, 631, 1162, 1207
TseI GCWGC 3 cut(s) 554, 587, 1136
TspDTI ATGAA 9 cut(s) 417, 470, 501, 646, 666, 795, 888, 1191, 1197
TspGWI ACGGA 4 cut(s) 72, 877, 938, 992
TspRI CASTG 4 cut(s) 347, 631, 1162, 1207
VpaK11BI GGWCC 3 cut(s) 99, 223, 232
XapI RAATTY 3 cut(s) 184, 404, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 1258
XmnI GAANNNNTTC 2 cut(s) 521, 1213
XspI CTAG 5 cut(s) 47, 528, 551, 1055, 1092
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.