RLG00000022418

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006057
Physical Location & Seq
Forward (+)
83215640 .. 83217477
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022418

Sequence Viewer

Length: 1251 bp
ATGGAAGAGAGTTTCAGCGTTCGAGTAGGCAAAACGTTCGATATTCTAGCTGGCTCATCACCATCACCGACAGCGAGGGCTCCGTCTTCGTCGCTGTGGTCCCTAACGGGTGAGGAAATCGAGAAGAGAGAATGGAACAGGGACAAGGGGAGCCCCGAACCCCAACCCCGACCCTGCAACCCAAATTCAAAGGATGATTTCTTCGGTAACGAGCTGGAGAAGGACCTTCTGGACCTTGACGACGACGATGACGTGGAAGAAGACGAGGTGGTGGAGGAGCAATCTGCTAAGCCTATGCCGGATGATTATAACGAGGAGCAGTGGCAAATTAAAACCTGCATTGGCTTGGACTGTACTCTTGACCACGAGGAAGAGGAAGATGAATTTGACAAAGTGGCTGTTGGCAAGGGAAATGCTGGAGACCGCTTTTATATGAAGGATGTAAATGACTATCCGATAGACATTGATTCTCAATACGAGGTTCCCAATTCATTGAACGATTTCACTAGAGACCCTCGCGCCAATCACCTAGCAGCTAAGATTAGACTCCAACAGGATGCAGAAGCAGCTCGAAAAATTGATTCCTTGCGGGTTTCTGGGAACGCTGCACCATCCAGTGCTATAGCTGATGAATTTGTCCCGTCTGAAGATGCTATCAACCTGAAATCAATTTTGAAGAGGAAGAATGATGAGCAGTCAGGCTCATCTAAATCACAAAAGCGTGTTCGGTTTGACCCTGAGTGTAAAGATGATTGCGATGAAGAGCCTGATGGATCCAAAGATATACCTGTGAAAAGCCTTTCAGGTGAAGATCATGTGTCTGCAGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATACATTTGATTCATCAACTGACATGGATGAGGAAGCTAACAGGCAAGCCTATATGGATTTCCGTAATCTTATGGGGATGTCAAATACTACGGATGCAGAAGCACCCAGGGATTTTTCAAAACCCGTCATATTCACACCGAAAAAGAAATCAACTGATGCGACAATGCTAGAAATTGATGGGGAGTTGGAGAGACCAGTAGGTGCTAGTAAGGAATCGGCGCCTTACAGAGTCGTGCCAATTGCTATTGCAGCCAGGGATCACGAAGACAGTGATGTGTGTGCAATGGATGAAGACGAACCAGATATAGTAACCAGTGGAAGAACAAGTTCACAGAGGATTGGCCGCCAGTTTCGTACAAGAACCAGTTTAGAGTTGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

417

Amino Acids

46.72

Weight (kDa)

4.42

Isoelectric Point (pI)

56.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 263 - 322 6.1e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 1084
AccII CGCG 1 cut(s) 519
AciI CCGC 3 cut(s) 424, 589, 1210
AclI AACGTT 1 cut(s) 35
AclWI GGATC 3 cut(s) 768, 781, 1131
AcoI YGGCCR 1 cut(s) 1207
AcsI RAATTY 3 cut(s) 184, 383, 632
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 1085
AfaI GTAC 2 cut(s) 355, 1222
AgsI TTSAA 4 cut(s) 189, 496, 676, 984
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 2 cut(s) 971, 1118
AluBI AGCT 6 cut(s) 50, 214, 536, 569, 626, 902
AluI AGCT 6 cut(s) 50, 214, 536, 569, 626, 902
Alw26I GTCTC 3 cut(s) 414, 504, 1051
AlwI GGATC 3 cut(s) 768, 781, 1131
AoxI GGCC 1 cut(s) 1207
ApeKI GCWGC 4 cut(s) 533, 566, 605, 1115
ApoI RAATTY 3 cut(s) 184, 383, 632
Asp700I GAANNNNTTC 2 cut(s) 500, 1192
AspLEI GCGC 2 cut(s) 521, 1087
AspS9I GGNCC 3 cut(s) 99, 223, 232
AsuHPI GGTGA 5 cut(s) 51, 57, 122, 518, 818
AvaII GGWCC 3 cut(s) 99, 223, 232
BamHI GGATCC 1 cut(s) 773
BanI GGYRCC 1 cut(s) 1084
BanII GRGCYC 2 cut(s) 82, 155
BauI CACGAG 1 cut(s) 365
BbsI GAAGAC 4 cut(s) 78, 267, 1137, 1164
BbvI GCAGC 4 cut(s) 545, 578, 592, 1127
BccI CCATC 5 cut(s) 70, 619, 764, 856, 1037
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 2 cut(s) 973, 1120
BcoDI GTCTC 3 cut(s) 414, 504, 1051
BfaI CTAG 5 cut(s) 47, 507, 530, 1034, 1071
BfmI CTRYAG 2 cut(s) 621, 822
BfoI RGCGCY 1 cut(s) 1088
BfuAI ACCTGC 1 cut(s) 344
BisI GCNGC 5 cut(s) 534, 567, 606, 1116, 1210
BlpI GCTNAGC 1 cut(s) 288
BlsI GCNGC 5 cut(s) 535, 568, 607, 1117, 1211
Bme1390I CCNGG 2 cut(s) 973, 1120
Bme18I GGWCC 3 cut(s) 99, 223, 232
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 99, 223, 232
BmiI GGNNCC 6 cut(s) 81, 101, 152, 483, 775, 1086
BmrFI CCNGG 2 cut(s) 973, 1120
BmsI GCATC 4 cut(s) 547, 640, 949, 1012
BpiI GAAGAC 4 cut(s) 78, 267, 1137, 1164
BpmI CTGGAG 2 cut(s) 236, 438
Bpu1102I GCTNAGC 1 cut(s) 288
BsaHI GRCGYC 1 cut(s) 1085
BsaI GGTCTC 3 cut(s) 414, 504, 1051
BsaJI CCNNGG 3 cut(s) 971, 972, 1119
Bse1I ACTGG 5 cut(s) 615, 1061, 1179, 1213, 1230
Bse3DI GCAATG 1 cut(s) 1155
BseBI CCWGG 2 cut(s) 973, 1120
BseDI CCNNGG 3 cut(s) 971, 972, 1119
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 1 cut(s) 729
BseNI ACTGG 5 cut(s) 615, 1061, 1179, 1213, 1230
BseRI GAGGAG 2 cut(s) 290, 329
BseXI GCAGC 4 cut(s) 545, 578, 592, 1127
BsgI GTGCAG 1 cut(s) 591
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 1 cut(s) 1209
BshNI GGYRCC 1 cut(s) 1084
BsiSI CCGG 1 cut(s) 299
BslFI GGGAC 3 cut(s) 85, 155, 623
BsmAI GTCTC 3 cut(s) 414, 504, 1051
BsmFI GGGAC 3 cut(s) 85, 155, 623
BsnI GGCC 1 cut(s) 1209
Bso31I GGTCTC 3 cut(s) 414, 504, 1051
Bsp1286I GDGCHC 2 cut(s) 82, 155
Bsp143I GATC 3 cut(s) 773, 811, 1123
Bsp1720I GCTNAGC 1 cut(s) 288
BspACI CCGC 3 cut(s) 424, 589, 1210
BspANI GGCC 1 cut(s) 1209
BspCNI CTCAG 1 cut(s) 730
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 6 cut(s) 81, 101, 152, 483, 775, 1086
BspMAI CTGCAG 1 cut(s) 826
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 3 cut(s) 768, 781, 1131
BspQI GCTCTTC 1 cut(s) 756
BspT107I GGYRCC 1 cut(s) 1084
BspTNI GGTCTC 3 cut(s) 414, 504, 1051
BsrDI GCAATG 1 cut(s) 1155
BsrI ACTGG 5 cut(s) 615, 1061, 1179, 1213, 1230
BssECI CCNNGG 3 cut(s) 971, 972, 1119
BssMI GATC 3 cut(s) 773, 811, 1123
BssNI GRCGYC 1 cut(s) 1085
BssSI CACGAG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 2 cut(s) 973, 1120
Bst4CI ACNGT 2 cut(s) 353, 1136
Bst6I CTCTTC 4 cut(s) 119, 366, 671, 756
BstACI GRCGYC 1 cut(s) 1085
BstC8I GCNNGC 2 cut(s) 52, 912
BstDEI CTNAG 3 cut(s) 288, 537, 738
BstFNI CGCG 1 cut(s) 519
BstH2I RGCGCY 1 cut(s) 1088
BstHHI GCGC 2 cut(s) 521, 1087
BstKTI GATC 3 cut(s) 776, 814, 1126
BstMAI GTCTC 3 cut(s) 414, 504, 1051
BstMBI GATC 3 cut(s) 773, 811, 1123
BstMWI GCNNNNNNNGC 2 cut(s) 566, 1115
BstNI CCWGG 2 cut(s) 973, 1120
BstSCI CCNGG 2 cut(s) 971, 1118
BstSFI CTRYAG 2 cut(s) 621, 822
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 4 cut(s) 545, 578, 592, 1127
BstV2I GAAGAC 4 cut(s) 78, 267, 1137, 1164
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BsuRI GGCC 1 cut(s) 1209
BtgZI GCGATG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 326
BtsIMutI CAGTG 4 cut(s) 326, 622, 1141, 1186
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 52, 912
CfoI GCGC 2 cut(s) 521, 1087
Cfr13I GGNCC 3 cut(s) 99, 223, 232
Csp6I GTAC 2 cut(s) 354, 1221
CviAII CATG 2 cut(s) 815, 889
CviQI GTAC 2 cut(s) 354, 1221
DdeI CTNAG 3 cut(s) 288, 537, 738
DinI GGCGCC 1 cut(s) 1086
DpnI GATC 3 cut(s) 775, 813, 1125
DpnII GATC 3 cut(s) 773, 811, 1123
EaeI YGGCCR 1 cut(s) 1207
Eam1104I CTCTTC 4 cut(s) 119, 366, 671, 756
EarI CTCTTC 4 cut(s) 119, 366, 671, 756
Eco24I GRGCYC 2 cut(s) 82, 155
Eco31I GGTCTC 3 cut(s) 414, 504, 1051
Eco47I GGWCC 3 cut(s) 99, 223, 232
Eco57I CTGAAG 1 cut(s) 666
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 971, 1118
EcoT38I GRGCYC 2 cut(s) 82, 155
EgeI GGCGCC 1 cut(s) 1086
EheI GGCGCC 1 cut(s) 1086
FaeI CATG 2 cut(s) 818, 892
FaqI GGGAC 3 cut(s) 85, 155, 623
FatI CATG 2 cut(s) 814, 888
FauI CCCGC 1 cut(s) 582
Fnu4HI GCNGC 5 cut(s) 534, 567, 606, 1116, 1210
FokI GGATG 9 cut(s) 206, 314, 452, 569, 598, 905, 955, 971, 1166
FriOI GRGCYC 2 cut(s) 82, 155
Fsp4HI GCNGC 5 cut(s) 534, 567, 606, 1116, 1210
FspBI CTAG 5 cut(s) 47, 507, 530, 1034, 1071
GlaI GCGC 2 cut(s) 520, 1086
GluI GCNGC 5 cut(s) 534, 567, 606, 1116, 1210
GsuI CTGGAG 2 cut(s) 236, 438
HaeII RGCGCY 1 cut(s) 1088
HaeIII GGCC 1 cut(s) 1209
HapII CCGG 1 cut(s) 299
HhaI GCGC 2 cut(s) 521, 1087
Hin1I GRCGYC 1 cut(s) 1085
Hin1II CATG 2 cut(s) 818, 892
Hin6I GCGC 2 cut(s) 519, 1085
HinP1I GCGC 2 cut(s) 519, 1085
HinfI GANTC 7 cut(s) 467, 546, 581, 844, 875, 1079, 1095
HpaII CCGG 1 cut(s) 299
HphI GGTGA 5 cut(s) 51, 57, 122, 518, 818
Hpy166II GTNNAC 1 cut(s) 1196
Hpy188I TCNGA 2 cut(s) 456, 646
Hpy188III TCNNGA 6 cut(s) 121, 230, 359, 830, 852, 1127
Hpy8I GTNNAC 1 cut(s) 1196
Hpy99I CGWCG 3 cut(s) 94, 245, 248
HpyAV CCTTC 3 cut(s) 214, 236, 430
HpyCH4III ACNGT 2 cut(s) 353, 1136
HpyCH4IV ACGT 2 cut(s) 35, 252
HpyCH4V TGCA 8 cut(s) 177, 339, 560, 608, 824, 962, 1115, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 566, 1115
HpyF3I CTNAG 3 cut(s) 288, 537, 738
HpySE526I ACGT 2 cut(s) 35, 252
Hsp92I GRCGYC 1 cut(s) 1085
Hsp92II CATG 2 cut(s) 818, 892
HspAI GCGC 2 cut(s) 519, 1085
KasI GGCGCC 1 cut(s) 1084
Kzo9I GATC 3 cut(s) 773, 811, 1123
LguI GCTCTTC 1 cut(s) 756
LmnI GCTCC 4 cut(s) 85, 150, 277, 316
Lsp1109I GCAGC 4 cut(s) 545, 578, 592, 1127
LweI GCATC 4 cut(s) 547, 640, 949, 1012
MaeI CTAG 5 cut(s) 47, 507, 530, 1034, 1071
MaeII ACGT 2 cut(s) 35, 252
MaeIII GTNAC 2 cut(s) 206, 1174
MalI GATC 3 cut(s) 775, 813, 1125
MboI GATC 3 cut(s) 773, 811, 1123
MfeI CAATTG 1 cut(s) 1104
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 2 cut(s) 82, 155
MluCI AATT 9 cut(s) 184, 327, 383, 487, 576, 632, 669, 1038, 1104
Mly113I GGCGCC 1 cut(s) 1085
MlyI GAGTC 2 cut(s) 540, 1104
MmeI TCCRAC 3 cut(s) 574, 1032, 1221
MroXI GAANNNNTTC 2 cut(s) 500, 1192
MseI TTAA 1 cut(s) 330
MspI CCGG 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 973, 1120
MunI CAATTG 1 cut(s) 1104
MvaI CCWGG 2 cut(s) 973, 1120
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 2 cut(s) 566, 1115
NarI GGCGCC 1 cut(s) 1085
NdeII GATC 3 cut(s) 773, 811, 1123
NlaIII CATG 2 cut(s) 818, 892
NlaIV GGNNCC 6 cut(s) 81, 101, 152, 483, 775, 1086
PasI CCCWGGG 1 cut(s) 972
PciSI GCTCTTC 1 cut(s) 756
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PdmI GAANNNNTTC 2 cut(s) 500, 1192
PfeI GAWTC 5 cut(s) 467, 581, 844, 875, 1079
PkrI GCNGC 5 cut(s) 535, 568, 607, 1117, 1211
PleI GAGTC 2 cut(s) 540, 1103
PluTI GGCGCC 1 cut(s) 1088
PpsI GAGTC 2 cut(s) 540, 1103
PpuMI RGGWCCY 1 cut(s) 223
PsiI TTATAA 1 cut(s) 309
Psp1406I AACGTT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 2 cut(s) 971, 1118
PspGI CCWGG 2 cut(s) 971, 1118
PspN4I GGNNCC 6 cut(s) 81, 101, 152, 483, 775, 1086
PspPI GGNCC 3 cut(s) 99, 223, 232
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 1 cut(s) 826
PsuI RGATCY 1 cut(s) 773
RsaI GTAC 2 cut(s) 355, 1222
RsaNI GTAC 2 cut(s) 354, 1221
SapI GCTCTTC 1 cut(s) 756
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 5 cut(s) 534, 567, 606, 1116, 1210
Sau3AI GATC 3 cut(s) 773, 811, 1123
Sau96I GGNCC 3 cut(s) 99, 223, 232
SchI GAGTC 2 cut(s) 540, 1104
ScrFI CCNGG 2 cut(s) 973, 1120
SduI GDGCHC 2 cut(s) 82, 155
SfaNI GCATC 4 cut(s) 547, 640, 949, 1012
SfcI CTRYAG 2 cut(s) 621, 822
SfoI GGCGCC 1 cut(s) 1086
SinI GGWCC 3 cut(s) 99, 223, 232
Sse9I AATT 9 cut(s) 184, 327, 383, 487, 576, 632, 669, 1038, 1104
SsiI CCGC 3 cut(s) 424, 589, 1210
SspDI GGCGCC 1 cut(s) 1084
SspMI CTAG 5 cut(s) 47, 507, 530, 1034, 1071
StyD4I CCNGG 2 cut(s) 971, 1118
TaaI ACNGT 2 cut(s) 353, 1136
TaiI ACGT 2 cut(s) 38, 255
TaqI TCGA 4 cut(s) 22, 39, 120, 571
TasI AATT 9 cut(s) 184, 327, 383, 487, 576, 632, 669, 1038, 1104
TatI WGTACW 1 cut(s) 353
TauI GCSGC 1 cut(s) 1212
TfiI GAWTC 5 cut(s) 467, 581, 844, 875, 1079
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 4 cut(s) 326, 622, 1141, 1186
TseI GCWGC 4 cut(s) 533, 566, 605, 1115
TspDTI ATGAA 7 cut(s) 396, 449, 480, 645, 774, 867, 1170
TspGWI ACGGA 4 cut(s) 72, 856, 917, 971
TspRI CASTG 4 cut(s) 326, 622, 1141, 1186
VpaK11BI GGWCC 3 cut(s) 99, 223, 232
XapI RAATTY 3 cut(s) 184, 383, 632
XcmI CCANNNNNNNNNTGG 1 cut(s) 1237
XmnI GAANNNNTTC 2 cut(s) 500, 1192
XspI CTAG 5 cut(s) 47, 507, 530, 1034, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.