MD17G1006900.v1.1

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006057
Physical Location & Seq
Reverse (-)
461487 .. 463256
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1006900.v1.1.491

Sequence Viewer

Length: 1278 bp
ATGGAAGACACGTTCAACGTTCGAGTAGGCAAAATCTTCGGCTCTCTCCCAGTTTCATCGTCGTCGTCCGGTCAACAGTCGTGTCTGAGCTCGCTATGGTCCCTAACCGACGAGGAAATCGAGAGGAGGGAATGGAACAGAGACAAGGGCAGCCCCGAACCCGAACCCGAACCCCTACCCTTCTACTCAAAATCAAATTCAAGGTCGAAGAATGATTTCAGTGACGACCTGGAGAAGGACCTTCTAGACCTTGACGACGACGACGTCGAAGACGAAGACGAAGGCGAGGAGGAGCAAGAGGCATCTGGTTCTCCCAGTCAGCCCGCAACTGAGGATAAGCCAGACGGCTACAATGAAGAGGAGTGGGAAATTAAATCCTCAATCGGCAGGGACTGTACTCTCGACTTCGAGGAAGAGGAAGATGGATATGACAAAGTGGCTGTTGGCAAGGAGACCGCCGGAGATCGCTTGTATATGAGGGATGTCAACGACTATGGGATCGATATTGATACTCAAGAGAAGGTTCCTAGTTCCATTAAGGATTTCACTAGAGACCCGCGTGCTAATCACCTGGCAGCTAAGATTAGGCTCCAACAAGATGCTGAAGCAGCTCAAAAAAGTGACTCCCTCAGGGTTTCTGGGAACGGCACATCGTCTTCTATAGCTGCTGAAAACATCTCTGAAGATGCTGCCAACCTAAAGTCGATTCTGAAGAGGAAGAATGATAATCAGTTAGACTCGTCCAAAACACAAAAACGTGTCCGGTTTGACTCCAACTGCAAAAGTAATGAAGATGAAGACGGAGCCAAAGATGTTCCTGTAGAAACTCATTCAAATGAAAATCCTCCAGTTCCGGATTATATACGAAATCCTTCAAGATATACGCATTACACGTTTGATTCATCAGGTGATGTGGACGAGGAGTCTAACAAGCAAGCCTACATGGAATTCCTTAATCTGGTGAGGAAGTCAAATTCTACGGAACCACAGGCAGAGGATGCTTCTGTTGATCTTTCAAAACCTGTCACATTTATACCAAGAAAGAAATCAAGTGATGCTTTAATGATAGAAAACGATGGGGAGTCAGAGAGACCAGGGGGTGCTCGAAGGGAAACTGCGGCTTGCAAAGTGATGCCACTTGCCATTGCAGCAGAGGATAATGAAGATGTTTGTGCAATGGAGGAAGATGAACCGGATACAGCAATGGATGGAAGAAGCAGTATACAGAGAACTGGACGCCAATACCGTACAAAAACCAGTTTGGGATTGGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

426

Amino Acids

47.43

Weight (kDa)

4.38

Isoelectric Point (pI)

64.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 269 - 333 4.5e-08 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AatII GACGTC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 1222
AccII CGCG 1 cut(s) 559
AccIII TCCGGA 1 cut(s) 853
AciI CCGC 4 cut(s) 324, 456, 557, 1118
AclI AACGTT 1 cut(s) 18
AclWI GGATC 1 cut(s) 506
AcsI RAATTY 3 cut(s) 196, 947, 973
AcuI CTGAAG 3 cut(s) 624, 702, 731
AcyI GRCGYC 2 cut(s) 264, 1237
AfaI GTAC 2 cut(s) 397, 1249
AfiI CCNNNNNNNGG 2 cut(s) 235, 958
AflIII ACRYGT 3 cut(s) 9, 757, 891
AgsI TTSAA 5 cut(s) 16, 201, 834, 876, 1017
AhdI GACNNNNNGTC 1 cut(s) 922
AjnI CCWGG 3 cut(s) 228, 570, 1093
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 90, 578, 611, 665
AluI AGCT 4 cut(s) 90, 578, 611, 665
Alw21I GWGCWC 2 cut(s) 92, 1105
Alw26I GTCTC 4 cut(s) 135, 446, 546, 1084
AlwI GGATC 1 cut(s) 506
AlwNI CAGNNNCTG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 853
ApeKI GCWGC 6 cut(s) 150, 575, 608, 665, 689, 1148
ApoI RAATTY 3 cut(s) 196, 947, 973
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 2 cut(s) 215, 871
AspS9I GGNCC 2 cut(s) 99, 238
AsuHPI GGTGA 3 cut(s) 560, 920, 973
AvaII GGWCC 2 cut(s) 99, 238
AxyI CCTNAGG 1 cut(s) 629
BanII GRGCYC 1 cut(s) 92
BbsI GAAGAC 5 cut(s) 12, 276, 282, 648, 804
Bbv12I GWGCWC 2 cut(s) 92, 1105
BbvI GCAGC 6 cut(s) 162, 587, 620, 652, 676, 1160
BccI CCATC 3 cut(s) 416, 1070, 1202
BceAI ACGGC 2 cut(s) 361, 661
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 3 cut(s) 230, 572, 1095
BciVI GTATCC 1 cut(s) 1189
BcoDI GTCTC 4 cut(s) 135, 446, 546, 1084
BfaI CTAG 3 cut(s) 245, 528, 549
BfmI CTRYAG 2 cut(s) 660, 819
BfuI GTATCC 1 cut(s) 1189
BisI GCNGC 7 cut(s) 151, 576, 609, 666, 690, 1119, 1149
BlsI GCNGC 7 cut(s) 152, 577, 610, 667, 691, 1120, 1150
Bme1390I CCNGG 3 cut(s) 230, 572, 1095
Bme18I GGWCC 2 cut(s) 99, 238
BmeRI GACNNNNNGTC 1 cut(s) 922
BmgT120I GGNCC 2 cut(s) 99, 238
BmiI GGNNCC 5 cut(s) 101, 525, 590, 805, 984
BmrFI CCNGG 3 cut(s) 230, 572, 1095
BmrI ACTGGG 2 cut(s) 44, 309
BmsI GCATC 6 cut(s) 311, 589, 676, 988, 1045, 1122
BmuI ACTGGG 2 cut(s) 44, 309
BpiI GAAGAC 5 cut(s) 12, 276, 282, 648, 804
BpmI CTGGAG 2 cut(s) 251, 831
BpuEI CTTGAG 1 cut(s) 498
Bsa29I ATCGAT 1 cut(s) 501
BsaHI GRCGYC 2 cut(s) 264, 1237
BsaI GGTCTC 3 cut(s) 446, 546, 1084
BsaJI CCNNGG 1 cut(s) 1094
BsaWI WCCGGW 4 cut(s) 68, 762, 853, 1192
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 958
Bse1I ACTGG 5 cut(s) 50, 315, 848, 1237, 1257
Bse21I CCTNAGG 1 cut(s) 629
Bse3DI GCAATG 3 cut(s) 1143, 1182, 1209
BseAI TCCGGA 1 cut(s) 853
BseBI CCWGG 3 cut(s) 230, 572, 1095
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 1 cut(s) 1094
BseGI GGATG 4 cut(s) 487, 1003, 1213, 1276
BseLI CCNNNNNNNGG 2 cut(s) 235, 958
BseMI GCAATG 3 cut(s) 1143, 1182, 1209
BseMII CTCAG 3 cut(s) 77, 321, 643
BseNI ACTGG 5 cut(s) 50, 315, 848, 1237, 1257
BseRI GAGGAG 5 cut(s) 139, 302, 305, 374, 935
BseXI GCAGC 6 cut(s) 162, 587, 620, 652, 676, 1160
Bsh1236I CGCG 1 cut(s) 559
BshVI ATCGAT 1 cut(s) 501
BsiHKAI GWGCWC 2 cut(s) 92, 1105
BsiSI CCGG 5 cut(s) 69, 459, 763, 854, 1193
BslFI GGGAC 2 cut(s) 85, 404
BslI CCNNNNNNNGG 2 cut(s) 235, 958
BsmAI GTCTC 4 cut(s) 135, 446, 546, 1084
BsmFI GGGAC 2 cut(s) 85, 404
Bso31I GGTCTC 3 cut(s) 446, 546, 1084
Bsp1286I GDGCHC 2 cut(s) 92, 1105
Bsp13I TCCGGA 1 cut(s) 853
Bsp143I GATC 3 cut(s) 463, 498, 1009
BspACI CCGC 4 cut(s) 324, 456, 557, 1118
BspCNI CTCAG 3 cut(s) 78, 322, 642
BspDI ATCGAT 1 cut(s) 501
BspEI TCCGGA 1 cut(s) 853
BspFNI CGCG 1 cut(s) 559
BspLI GGNNCC 5 cut(s) 101, 525, 590, 805, 984
BspPI GGATC 1 cut(s) 506
BspTNI GGTCTC 3 cut(s) 446, 546, 1084
BsrDI GCAATG 3 cut(s) 1143, 1182, 1209
BsrI ACTGG 5 cut(s) 50, 315, 848, 1237, 1257
BssECI CCNNGG 1 cut(s) 1094
BssMI GATC 3 cut(s) 463, 498, 1009
BssNAI GTATAC 1 cut(s) 1223
BssNI GRCGYC 2 cut(s) 264, 1237
Bst1107I GTATAC 1 cut(s) 1223
Bst2UI CCWGG 3 cut(s) 230, 572, 1095
Bst4CI ACNGT 3 cut(s) 78, 395, 1247
Bst6I CTCTTC 3 cut(s) 351, 408, 707
BstACI GRCGYC 2 cut(s) 264, 1237
BstAPI GCANNNNNTGC 1 cut(s) 998
BstC8I GCNNGC 5 cut(s) 92, 324, 561, 936, 1123
BstDEI CTNAG 4 cut(s) 86, 330, 579, 629
BstENI CCTNNNNNAGG 1 cut(s) 233
BstF5I GGATG 4 cut(s) 487, 1003, 1213, 1276
BstFNI CGCG 1 cut(s) 559
BstKTI GATC 3 cut(s) 466, 501, 1012
BstMAI GTCTC 4 cut(s) 135, 446, 546, 1084
BstMBI GATC 3 cut(s) 463, 498, 1009
BstMWI GCNNNNNNNGC 3 cut(s) 608, 998, 1148
BstNI CCWGG 3 cut(s) 230, 572, 1095
BstSCI CCNGG 3 cut(s) 228, 570, 1093
BstSFI CTRYAG 2 cut(s) 660, 819
BstUI CGCG 1 cut(s) 559
BstV1I GCAGC 6 cut(s) 162, 587, 620, 652, 676, 1160
BstV2I GAAGAC 5 cut(s) 12, 276, 282, 648, 804
BstZ17I GTATAC 1 cut(s) 1223
Bsu15I ATCGAT 1 cut(s) 501
Bsu36I CCTNAGG 1 cut(s) 629
BsuI GTATCC 1 cut(s) 1189
BsuTUI ATCGAT 1 cut(s) 501
BtsCI GGATG 4 cut(s) 487, 1003, 1213, 1276
BtsIMutI CAGTG 1 cut(s) 226
Cac8I GCNNGC 5 cut(s) 92, 324, 561, 936, 1123
CaiI CAGNNNCTG 1 cut(s) 393
Cfr13I GGNCC 2 cut(s) 99, 238
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 1 cut(s) 1245
Csp6I GTAC 2 cut(s) 396, 1248
CviAII CATG 1 cut(s) 943
CviQI GTAC 2 cut(s) 396, 1248
DdeI CTNAG 4 cut(s) 86, 330, 579, 629
DpnI GATC 3 cut(s) 465, 500, 1011
DpnII GATC 3 cut(s) 463, 498, 1009
DrdI GACNNNNNNGTC 1 cut(s) 263
DriI GACNNNNNGTC 1 cut(s) 922
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 3 cut(s) 351, 408, 707
Eam1105I GACNNNNNGTC 1 cut(s) 922
EarI CTCTTC 3 cut(s) 351, 408, 707
Ecl136II GAGCTC 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 92
Eco31I GGTCTC 3 cut(s) 446, 546, 1084
Eco47I GGWCC 2 cut(s) 99, 238
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 3 cut(s) 624, 702, 731
Eco81I CCTNAGG 1 cut(s) 629
EcoICRI GAGCTC 1 cut(s) 90
EcoNI CCTNNNNNAGG 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 238
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 3 cut(s) 228, 570, 1093
EcoT38I GRGCYC 1 cut(s) 92
FaeI CATG 1 cut(s) 946
FalI AAGNNNNNCTT 2 cut(s) 1042, 1074
FaqI GGGAC 2 cut(s) 85, 404
FatI CATG 1 cut(s) 942
FauI CCCGC 2 cut(s) 331, 564
FblI GTMKAC 1 cut(s) 1222
Fnu4HI GCNGC 7 cut(s) 151, 576, 609, 666, 690, 1119, 1149
FokI GGATG 3 cut(s) 494, 1010, 1220
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 7 cut(s) 151, 576, 609, 666, 690, 1119, 1149
FspBI CTAG 3 cut(s) 245, 528, 549
GluI GCNGC 7 cut(s) 151, 576, 609, 666, 690, 1119, 1149
GsuI CTGGAG 2 cut(s) 251, 831
HapII CCGG 5 cut(s) 69, 459, 763, 854, 1193
HgaI GACGC 1 cut(s) 1245
Hin1I GRCGYC 2 cut(s) 264, 1237
Hin1II CATG 1 cut(s) 946
HincII GTYRAC 2 cut(s) 74, 487
HindII GTYRAC 2 cut(s) 74, 487
HinfI GANTC 7 cut(s) 623, 706, 737, 770, 899, 923, 1082
HpaII CCGG 5 cut(s) 69, 459, 763, 854, 1193
HphI GGTGA 3 cut(s) 560, 920, 973
Hpy166II GTNNAC 4 cut(s) 74, 487, 916, 1223
Hpy188I TCNGA 4 cut(s) 87, 682, 711, 1087
Hpy188III TCNNGA 6 cut(s) 121, 245, 401, 515, 854, 876
Hpy8I GTNNAC 4 cut(s) 74, 487, 916, 1223
Hpy99I CGWCG 7 cut(s) 64, 67, 113, 260, 263, 266, 269
HpyAV CCTTC 7 cut(s) 190, 229, 251, 275, 514, 882, 1101
HpyCH4III ACNGT 3 cut(s) 78, 395, 1247
HpyCH4IV ACGT 5 cut(s) 11, 18, 264, 757, 893
HpyCH4V TGCA 4 cut(s) 780, 1125, 1148, 1175
HpyF10VI GCNNNNNNNGC 3 cut(s) 608, 998, 1148
HpyF3I CTNAG 4 cut(s) 86, 330, 579, 629
HpySE526I ACGT 5 cut(s) 11, 18, 264, 757, 893
Hsp92I GRCGYC 2 cut(s) 264, 1237
Hsp92II CATG 1 cut(s) 946
Kpn2I TCCGGA 1 cut(s) 853
Kzo9I GATC 3 cut(s) 463, 498, 1009
LmnI GCTCC 3 cut(s) 292, 594, 803
Lsp1109I GCAGC 6 cut(s) 162, 587, 620, 652, 676, 1160
LweI GCATC 6 cut(s) 311, 589, 676, 988, 1045, 1122
MaeI CTAG 3 cut(s) 245, 528, 549
MaeII ACGT 5 cut(s) 11, 18, 264, 757, 893
MaeIII GTNAC 3 cut(s) 221, 620, 1024
MalI GATC 3 cut(s) 465, 500, 1011
MboI GATC 3 cut(s) 463, 498, 1009
MhlI GDGCHC 2 cut(s) 92, 1105
MluCI AATT 4 cut(s) 196, 369, 947, 973
MlyI GAGTC 5 cut(s) 617, 731, 764, 932, 1091
MmeI TCCRAC 2 cut(s) 616, 798
MroI TCCGGA 1 cut(s) 853
MroXI GAANNNNTTC 2 cut(s) 215, 871
MseI TTAA 4 cut(s) 372, 537, 954, 1061
MslI CAYNNNNRTG 1 cut(s) 834
MspI CCGG 5 cut(s) 69, 459, 763, 854, 1193
MspR9I CCNGG 3 cut(s) 230, 572, 1095
MvaI CCWGG 3 cut(s) 230, 572, 1095
MvnI CGCG 1 cut(s) 559
MwoI GCNNNNNNNGC 3 cut(s) 608, 998, 1148
NdeII GATC 3 cut(s) 463, 498, 1009
NlaIII CATG 1 cut(s) 946
NlaIV GGNNCC 5 cut(s) 101, 525, 590, 805, 984
NmuCI GTSAC 3 cut(s) 221, 620, 1024
PcsI WCGNNNNNNNCGW 6 cut(s) 117, 261, 264, 270, 890, 1243
PdmI GAANNNNTTC 2 cut(s) 215, 871
PfeI GAWTC 2 cut(s) 706, 899
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 7 cut(s) 152, 577, 610, 667, 691, 1120, 1150
PleI GAGTC 5 cut(s) 617, 731, 764, 931, 1090
PpsI GAGTC 5 cut(s) 617, 731, 764, 931, 1090
PpuMI RGGWCCY 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 92
Psp1406I AACGTT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 3 cut(s) 228, 570, 1093
PspGI CCWGG 3 cut(s) 228, 570, 1093
PspN4I GGNNCC 5 cut(s) 101, 525, 590, 805, 984
PspPI GGNCC 2 cut(s) 99, 238
PspPPI RGGWCCY 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 393
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 2 cut(s) 397, 1249
RsaNI GTAC 2 cut(s) 396, 1248
RseI CAYNNNNRTG 1 cut(s) 834
SacI GAGCTC 1 cut(s) 92
SaqAI TTAA 4 cut(s) 372, 537, 954, 1061
SatI GCNGC 7 cut(s) 151, 576, 609, 666, 690, 1119, 1149
Sau3AI GATC 3 cut(s) 463, 498, 1009
Sau96I GGNCC 2 cut(s) 99, 238
SchI GAGTC 5 cut(s) 617, 731, 764, 932, 1091
ScrFI CCNGG 3 cut(s) 230, 572, 1095
SduI GDGCHC 2 cut(s) 92, 1105
SfaNI GCATC 6 cut(s) 311, 589, 676, 988, 1045, 1122
SfcI CTRYAG 2 cut(s) 660, 819
SinI GGWCC 2 cut(s) 99, 238
SmiMI CAYNNNNRTG 1 cut(s) 834
SmlI CTYRAG 1 cut(s) 513
SmoI CTYRAG 1 cut(s) 513
Sse9I AATT 4 cut(s) 196, 369, 947, 973
SsiI CCGC 4 cut(s) 324, 456, 557, 1118
SspMI CTAG 3 cut(s) 245, 528, 549
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 3 cut(s) 228, 570, 1093
TaaI ACNGT 3 cut(s) 78, 395, 1247
TaiI ACGT 5 cut(s) 14, 21, 267, 760, 896
TaqI TCGA 9 cut(s) 22, 120, 206, 267, 402, 408, 501, 704, 1105
TasI AATT 4 cut(s) 196, 369, 947, 973
TatI WGTACW 1 cut(s) 395
TauI GCSGC 1 cut(s) 1121
TfiI GAWTC 2 cut(s) 706, 899
Tru1I TTAA 4 cut(s) 372, 537, 954, 1061
Tru9I TTAA 4 cut(s) 372, 537, 954, 1061
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 3 cut(s) 221, 620, 1024
TseI GCWGC 6 cut(s) 150, 575, 608, 665, 689, 1148
Tsp45I GTSAC 3 cut(s) 221, 620, 1024
TspDTI ATGAA 8 cut(s) 45, 369, 804, 810, 852, 891, 1176, 1203
TspGWI ACGGA 2 cut(s) 816, 995
TspRI CASTG 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 99, 238
XagI CCTNNNNNAGG 1 cut(s) 233
XapI RAATTY 3 cut(s) 196, 947, 973
XbaI TCTAGA 1 cut(s) 244
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XmiI GTMKAC 1 cut(s) 1222
XmnI GAANNNNTTC 2 cut(s) 215, 871
XspI CTAG 3 cut(s) 245, 528, 549
ZraI GACGTC 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.