MD09G1002700.v1.1

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006057
Physical Location & Seq
Reverse (-)
283931 .. 285638
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1002700.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGGAAGACACGTTTAGGGTTCGAGTAGGCAAAATCTTCGGCTCGCTAGCCGGTTCATCGCCATCGCCGTCGCCATCGAGCGGTCCACCGTCGTCTCTGAGTTCGCTATGGTCCCTAACAGACGAGGAAATGGAGAGGAGGGAATGGAACAGAGACAAGGACAGCCCCGAACCCGAAGCTGAACCCCTACCCTTCTACTCAAAATCAAAGTCAAAGAATGATTTCAGTGACGACCTGGAGGAGGACCTTCTAGACCTTGACGACGACTTGGAAGACAAAGAAGAAGACGAGGAGGAAGATGCATCTCCTTCTTCTTCCAGTCAGCCTGCAACAAAGCCCAAGCCAGACGACTACAACGAAGAGGAGTGGGAAATTAAATCCTCAATCGGCTGGGACTGTACTCTCGACTTCGAGGATGAGGAAGATGGATATGACAAAGTGGCTGTTGGCAAGGAAACCGCTGGAGATCGCTTGTATATGAGGGATGTCAACGACTATGGTATCGACATAGACACTCAAGATGAGGTTCCTAGTTCCATCAAGGATTTCACTAGAGACCCACGTGCCAATCACCTGGCAGCTAAGATTAGGCTCCAACAAGATGCTGAAGCAGCTCAAAAAATTGACTCCCTCAGGGTTTCTGGGAACGGCGCACCATCTGCCATAGCTGCTGAAAACATCTCTGAAGATGCTCCCAACCCGAAGTCGATTCTGAAGAGGAAGAATGATAATCAGTTAGACTCGTCTAAAACACAAAAACGTGTCCGGTTTGACTCCAACTGCAAAAGTAATGAAGATGTAGAGGGTGAGGGAGCCAAAGATGTTACTGTAGAAACTCATTCAAATGAAAATCCTGCAGTTCCGGATTATATACGAAATCCTTCAAGATATACGCATTATACGTTTGATTCATCAGGTGACGTAGATGAGGAGTCTAACAAGCAAGCCTATATGGACTTCCTTAATCTGGTGAGGAAATCAAATTCTATGGAACCACAGGCTGAGGATGCTTCTGTTGATCTTTCAAAACCTGTCACATTTATACCGAGAAAGAAATCAAGCGATGCTGTAATGATAGAATACGATGGGGAGTCAGAGAGACCAGGAGGTGATAGAAGGGAATCTGCGGCTTGTAAAGTGATGCCACTTGCCATTGCAGCGGAGGATAACGAAGATGTTTGCGCAATGGAGGAAGATGAACCGGATACAACAACGGATGGAAGAAGCAGTATACAGAGAACTGGACGCCAATACCGGACAAAAACCAGTTTGGAATTCGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

429

Amino Acids

47.85

Weight (kDa)

4.34

Isoelectric Point (pI)

62.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 265 - 336 3.8e-08 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1183
AccBSI CCGCTC 1 cut(s) 81
AccI GTMKAC 1 cut(s) 1231
AccIII TCCGGA 1 cut(s) 862
AciI CCGC 4 cut(s) 81, 459, 1127, 1160
AcsI RAATTY 2 cut(s) 982, 1274
AcuI CTGAAG 3 cut(s) 627, 705, 734
AcvI CACGTG 1 cut(s) 563
AcyI GRCGYC 1 cut(s) 1246
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 3 cut(s) 80, 241, 967
AflIII ACRYGT 2 cut(s) 9, 760
AgsI TTSAA 3 cut(s) 843, 885, 1026
AjnI CCWGG 3 cut(s) 234, 573, 1102
AluBI AGCT 4 cut(s) 179, 581, 614, 668
AluI AGCT 4 cut(s) 179, 581, 614, 668
Alw26I GTCTC 4 cut(s) 99, 147, 549, 1093
Aor13HI TCCGGA 1 cut(s) 862
ApeKI GCWGC 4 cut(s) 578, 611, 668, 1157
ApoI RAATTY 2 cut(s) 982, 1274
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
Asp700I GAANNNNTTC 2 cut(s) 221, 880
AspLEI GCGC 2 cut(s) 653, 1184
AspS9I GGNCC 3 cut(s) 83, 111, 244
AsuHPI GGTGA 5 cut(s) 563, 818, 929, 982, 1121
AsuNHI GCTAGC 1 cut(s) 46
AvaII GGWCC 3 cut(s) 83, 111, 244
AxyI CCTNAGG 1 cut(s) 632
BaeI ACNNNNGTAYC 2 cut(s) 484, 517
BbrPI CACGTG 1 cut(s) 563
BbsI GAAGAC 3 cut(s) 12, 279, 291
BbvCI CCTCAGC 1 cut(s) 1002
BbvI GCAGC 4 cut(s) 590, 623, 655, 1169
BccI CCATC 7 cut(s) 70, 82, 419, 545, 664, 1079, 1211
BceAI ACGGC 2 cut(s) 52, 664
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 3 cut(s) 236, 575, 1104
BciVI GTATCC 1 cut(s) 1198
BcoDI GTCTC 4 cut(s) 99, 147, 549, 1093
BfaI CTAG 4 cut(s) 47, 251, 531, 552
BfmI CTRYAG 2 cut(s) 828, 855
BfuI GTATCC 1 cut(s) 1198
BisI GCNGC 5 cut(s) 579, 612, 669, 1128, 1158
BlsI GCNGC 5 cut(s) 580, 613, 670, 1129, 1159
Bme1390I CCNGG 3 cut(s) 236, 575, 1104
Bme18I GGWCC 3 cut(s) 83, 111, 244
BmgT120I GGNCC 3 cut(s) 83, 111, 244
BmiI GGNNCC 5 cut(s) 113, 528, 593, 814, 993
BmrFI CCNGG 3 cut(s) 236, 575, 1104
BmsI GCATC 7 cut(s) 289, 311, 592, 679, 997, 1054, 1131
BmtI GCTAGC 1 cut(s) 50
BpiI GAAGAC 3 cut(s) 12, 279, 291
BpmI CTGGAG 2 cut(s) 257, 483
Bpu10I CCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 501
BsaAI YACGTR 1 cut(s) 563
BsaHI GRCGYC 1 cut(s) 1246
BsaI GGTCTC 2 cut(s) 549, 1093
BsaWI WCCGGW 4 cut(s) 765, 862, 1201, 1254
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 241, 967
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 318, 1246, 1266
Bse21I CCTNAGG 1 cut(s) 632
Bse3DI GCAATG 2 cut(s) 1152, 1191
BseAI TCCGGA 1 cut(s) 862
BseBI CCWGG 3 cut(s) 236, 575, 1104
BseGI GGATG 4 cut(s) 421, 490, 1012, 1222
BseLI CCNNNNNNNGG 3 cut(s) 80, 241, 967
BseMI GCAATG 2 cut(s) 1152, 1191
BseMII CTCAG 3 cut(s) 89, 646, 993
BseNI ACTGG 3 cut(s) 318, 1246, 1266
BseRI GAGGAG 5 cut(s) 151, 254, 305, 377, 944
BseXI GCAGC 4 cut(s) 590, 623, 655, 1169
BseYI CCCAGC 1 cut(s) 390
BsiSI CCGG 5 cut(s) 51, 766, 863, 1202, 1255
BslFI GGGAC 2 cut(s) 97, 407
BslI CCNNNNNNNGG 3 cut(s) 80, 241, 967
BsmAI GTCTC 4 cut(s) 99, 147, 549, 1093
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 97, 407
Bso31I GGTCTC 2 cut(s) 549, 1093
Bsp13I TCCGGA 1 cut(s) 862
Bsp143I GATC 2 cut(s) 466, 1018
BspACI CCGC 4 cut(s) 81, 459, 1127, 1160
BspCNI CTCAG 3 cut(s) 90, 645, 994
BspEI TCCGGA 1 cut(s) 862
BspLI GGNNCC 5 cut(s) 113, 528, 593, 814, 993
BspMAI CTGCAG 1 cut(s) 859
BspOI GCTAGC 1 cut(s) 50
BspTNI GGTCTC 2 cut(s) 549, 1093
BsrBI CCGCTC 1 cut(s) 81
BsrDI GCAATG 2 cut(s) 1152, 1191
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 3 cut(s) 318, 1246, 1266
BssAI RCCGGY 1 cut(s) 50
BssMI GATC 2 cut(s) 466, 1018
BssNAI GTATAC 1 cut(s) 1232
BssNI GRCGYC 1 cut(s) 1246
Bst1107I GTATAC 1 cut(s) 1232
Bst2UI CCWGG 3 cut(s) 236, 575, 1104
Bst4CI ACNGT 3 cut(s) 90, 398, 829
Bst6I CTCTTC 2 cut(s) 354, 710
BstACI GRCGYC 1 cut(s) 1246
BstAPI GCANNNNNTGC 1 cut(s) 659
BstBAI YACGTR 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 44, 48, 327, 945
BstDEI CTNAG 4 cut(s) 98, 582, 632, 1002
BstENI CCTNNNNNAGG 1 cut(s) 239
BstF5I GGATG 4 cut(s) 421, 490, 1012, 1222
BstHHI GCGC 2 cut(s) 653, 1184
BstKTI GATC 2 cut(s) 469, 1021
BstMAI GTCTC 4 cut(s) 99, 147, 549, 1093
BstMBI GATC 2 cut(s) 466, 1018
BstMWI GCNNNNNNNGC 5 cut(s) 611, 659, 668, 1007, 1157
BstNI CCWGG 3 cut(s) 236, 575, 1104
BstSCI CCNGG 3 cut(s) 234, 573, 1102
BstSFI CTRYAG 2 cut(s) 828, 855
BstV1I GCAGC 4 cut(s) 590, 623, 655, 1169
BstV2I GAAGAC 3 cut(s) 12, 279, 291
BstXI CCANNNNNNTGG 1 cut(s) 574
BstZ17I GTATAC 1 cut(s) 1232
Bsu36I CCTNAGG 1 cut(s) 632
BsuI GTATCC 1 cut(s) 1198
BtgZI GCGATG 3 cut(s) 42, 48, 1077
BtsCI GGATG 4 cut(s) 421, 490, 1012, 1222
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 4 cut(s) 44, 48, 327, 945
CfoI GCGC 2 cut(s) 653, 1184
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 3 cut(s) 83, 111, 244
CseI GACGC 1 cut(s) 1254
Csp6I GTAC 1 cut(s) 399
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 4 cut(s) 98, 582, 632, 1002
DpnI GATC 2 cut(s) 468, 1020
DpnII GATC 2 cut(s) 466, 1018
Eam1104I CTCTTC 2 cut(s) 354, 710
EarI CTCTTC 2 cut(s) 354, 710
Eco31I GGTCTC 2 cut(s) 549, 1093
Eco47I GGWCC 3 cut(s) 83, 111, 244
Eco57I CTGAAG 3 cut(s) 627, 705, 734
Eco72I CACGTG 1 cut(s) 563
Eco81I CCTNAGG 1 cut(s) 632
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 3 cut(s) 234, 573, 1102
EcoT22I ATGCAT 1 cut(s) 304
Esp3I CGTCTC 1 cut(s) 99
FaqI GGGAC 2 cut(s) 97, 407
FblI GTMKAC 1 cut(s) 1231
Fnu4HI GCNGC 5 cut(s) 579, 612, 669, 1128, 1158
FokI GGATG 4 cut(s) 428, 497, 1019, 1229
Fsp4HI GCNGC 5 cut(s) 579, 612, 669, 1128, 1158
FspBI CTAG 4 cut(s) 47, 251, 531, 552
FspI TGCGCA 1 cut(s) 1183
GlaI GCGC 2 cut(s) 652, 1183
GluI GCNGC 5 cut(s) 579, 612, 669, 1128, 1158
GsaI CCCAGC 1 cut(s) 394
GsuI CTGGAG 2 cut(s) 257, 483
HapII CCGG 5 cut(s) 51, 766, 863, 1202, 1255
HgaI GACGC 1 cut(s) 1254
HhaI GCGC 2 cut(s) 653, 1184
Hin1I GRCGYC 1 cut(s) 1246
Hin6I GCGC 2 cut(s) 651, 1182
HinP1I GCGC 2 cut(s) 651, 1182
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 8 cut(s) 626, 709, 740, 773, 908, 932, 1091, 1121
HpaII CCGG 5 cut(s) 51, 766, 863, 1202, 1255
HphI GGTGA 5 cut(s) 563, 818, 929, 982, 1121
Hpy166II GTNNAC 3 cut(s) 86, 490, 1232
Hpy188I TCNGA 4 cut(s) 99, 685, 714, 1096
Hpy188III TCNNGA 5 cut(s) 251, 404, 518, 863, 885
Hpy8I GTNNAC 3 cut(s) 86, 490, 1232
Hpy99I CGWCG 3 cut(s) 73, 94, 266
HpyAV CCTTC 5 cut(s) 202, 257, 318, 891, 1110
HpyCH4III ACNGT 3 cut(s) 90, 398, 829
HpyCH4IV ACGT 5 cut(s) 11, 562, 760, 902, 921
HpyCH4V TGCA 5 cut(s) 302, 329, 783, 857, 1157
HpyF10VI GCNNNNNNNGC 5 cut(s) 611, 659, 668, 1007, 1157
HpyF3I CTNAG 4 cut(s) 98, 582, 632, 1002
HpySE526I ACGT 5 cut(s) 11, 562, 760, 902, 921
Hsp92I GRCGYC 1 cut(s) 1246
HspAI GCGC 2 cut(s) 651, 1182
Kpn2I TCCGGA 1 cut(s) 862
Kzo9I GATC 2 cut(s) 466, 1018
LmnI GCTCC 3 cut(s) 597, 697, 812
Lsp1109I GCAGC 4 cut(s) 590, 623, 655, 1169
LweI GCATC 7 cut(s) 289, 311, 592, 679, 997, 1054, 1131
MaeI CTAG 4 cut(s) 47, 251, 531, 552
MaeII ACGT 5 cut(s) 11, 562, 760, 902, 921
MaeIII GTNAC 4 cut(s) 227, 823, 917, 1033
MalI GATC 2 cut(s) 468, 1020
MbiI CCGCTC 1 cut(s) 81
MboI GATC 2 cut(s) 466, 1018
MluCI AATT 4 cut(s) 372, 621, 982, 1274
MlyI GAGTC 5 cut(s) 620, 734, 767, 941, 1100
MmeI TCCRAC 2 cut(s) 619, 801
Mph1103I ATGCAT 1 cut(s) 304
MroI TCCGGA 1 cut(s) 862
MroXI GAANNNNTTC 2 cut(s) 221, 880
MseI TTAA 2 cut(s) 375, 963
MslI CAYNNNNRTG 1 cut(s) 843
MspA1I CMGCKG 2 cut(s) 461, 1160
MspI CCGG 5 cut(s) 51, 766, 863, 1202, 1255
MspR9I CCNGG 3 cut(s) 236, 575, 1104
MvaI CCWGG 3 cut(s) 236, 575, 1104
MwoI GCNNNNNNNGC 5 cut(s) 611, 659, 668, 1007, 1157
NdeII GATC 2 cut(s) 466, 1018
NheI GCTAGC 1 cut(s) 46
NlaIV GGNNCC 5 cut(s) 113, 528, 593, 814, 993
NmuCI GTSAC 3 cut(s) 227, 917, 1033
NsbI TGCGCA 1 cut(s) 1183
NsiI ATGCAT 1 cut(s) 304
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 354, 899
PdmI GAANNNNTTC 2 cut(s) 221, 880
PfeI GAWTC 3 cut(s) 709, 908, 1121
PkrI GCNGC 5 cut(s) 580, 613, 670, 1129, 1159
PleI GAGTC 5 cut(s) 620, 734, 767, 940, 1099
PmaCI CACGTG 1 cut(s) 563
PmlI CACGTG 1 cut(s) 563
PpsI GAGTC 5 cut(s) 620, 734, 767, 940, 1099
Ppu21I YACGTR 1 cut(s) 563
PpuMI RGGWCCY 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 244
Psp6I CCWGG 3 cut(s) 234, 573, 1102
PspCI CACGTG 1 cut(s) 563
PspFI CCCAGC 1 cut(s) 390
PspGI CCWGG 3 cut(s) 234, 573, 1102
PspN4I GGNNCC 5 cut(s) 113, 528, 593, 814, 993
PspPI GGNCC 3 cut(s) 83, 111, 244
PspPPI RGGWCCY 1 cut(s) 244
PstI CTGCAG 1 cut(s) 859
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
RseI CAYNNNNRTG 1 cut(s) 843
SaqAI TTAA 2 cut(s) 375, 963
SatI GCNGC 5 cut(s) 579, 612, 669, 1128, 1158
Sau3AI GATC 2 cut(s) 466, 1018
Sau96I GGNCC 3 cut(s) 83, 111, 244
SchI GAGTC 5 cut(s) 620, 734, 767, 941, 1100
ScrFI CCNGG 3 cut(s) 236, 575, 1104
SfaNI GCATC 7 cut(s) 289, 311, 592, 679, 997, 1054, 1131
SfcI CTRYAG 2 cut(s) 828, 855
SinI GGWCC 3 cut(s) 83, 111, 244
SmiMI CAYNNNNRTG 1 cut(s) 843
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 4 cut(s) 372, 621, 982, 1274
SsiI CCGC 4 cut(s) 81, 459, 1127, 1160
SspMI CTAG 4 cut(s) 47, 251, 531, 552
StyD4I CCNGG 3 cut(s) 234, 573, 1102
TaaI ACNGT 3 cut(s) 90, 398, 829
TaiI ACGT 5 cut(s) 14, 565, 763, 905, 924
TaqI TCGA 7 cut(s) 22, 77, 405, 411, 504, 707, 1278
TasI AATT 4 cut(s) 372, 621, 982, 1274
TatI WGTACW 1 cut(s) 398
TauI GCSGC 1 cut(s) 1130
TfiI GAWTC 3 cut(s) 709, 908, 1121
Tru1I TTAA 2 cut(s) 375, 963
Tru9I TTAA 2 cut(s) 375, 963
TscAI CASTG 1 cut(s) 232
TseFI GTSAC 3 cut(s) 227, 917, 1033
TseI GCWGC 4 cut(s) 578, 611, 668, 1157
Tsp45I GTSAC 3 cut(s) 227, 917, 1033
TspDTI ATGAA 5 cut(s) 45, 807, 861, 900, 1212
TspGWI ACGGA 1 cut(s) 1229
TspRI CASTG 1 cut(s) 232
VpaK11BI GGWCC 3 cut(s) 83, 111, 244
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 2 cut(s) 982, 1274
XbaI TCTAGA 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 1231
XmnI GAANNNNTTC 2 cut(s) 221, 880
XspI CTAG 4 cut(s) 47, 251, 531, 552
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.