Rh2AG673500

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006057
Physical Location & Seq
Forward (+)
88587504 .. 88589120
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG673500.1

Sequence Viewer

Length: 1278 bp
ATGGAGGAGAGTTTCAGCGTTCGAGTAAGCAAAACGTTCGATATTCTAGCTGGCTCATCACCATCAGCGACAGCGAGGGCTCCGTCTTCGTCGCTGTGGTCCCTAACAGGTGAGGAAATCGAGAAGAGAGAATGGAACAGGGACAAGGGGAGCCCCGAACCCCAACCCCGACCCTGCAACCCAGATTCAAATTCAAAGGATGATTTCTTCGGTAACGAGCTGGAGAAGGACCTTCTGGACCTTGACGACGACGACGATGACGTGGAAGAAGAAGAAGACGAGGTGGTGGAGGAGCAATCTGCTAAGCCTAAGCCGAAGCCGAAGCCGAAGCCTATGCCGGATGATTATAACGAGGAGCAGTGGCAAATTAAAACCTGCATTGGCTTGGACTGTACTCTCGACCACGAGGAAGAGGAAGATGAATTTGACAAAGTGGCTGTTGGCAAGGGAAATGCTGCAGACCGCTTTTATATGAAGGATGTAAATGACTATCCGATAGACATTGATTCTCAATACGAGGTTCCCAATTCATTGAACGATTGCACTAGAGACCCTCGCGCCAATCACCTAGCAGCTAAGATTAGACTCCAACTGGATGCAGAAGCAGCTCAAAAAATTGACTCCTTGCGGGTCTCTGGGAACACTGCACCATCCGGTGCTATAGCTGATGAATTCGTCCCGTCTGAAGATGCTATCAACCTGAAATCAATTTTGAAGAGGAAAAGTGACGAGCAGTTAGGCTCATCTAAATCACGAAAGCGTGTTCGGTTTGACCCTGAGTGTAAAGATGATTGTGATGAAGAGCCTGATGGGTCCAAAGATATACCTGTGAAAAGCCTTTCAGGTGAAGATCATGTGTCTGCAGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATACATTTGATTCATCAACTGACATGGATGAGGAAGCTAACAGGCAAGCCTATATGGATTTCCGTAATCTTTTGGGGATGTCAAATACTACGGATGCAGAAGTTTCAAAATCGATCATATTCACACCAAAAAAGAAATCAACTGATGCGACAATGCTAGAAATTGATGGGGAGTCGGAGAGACCAATAGGTGCTAATAAGGAATCTGCGCCTTACAGAGTCGTGCCAATTGCTATTGCAGCCAGGGATCACGAAGACAGTGATGTGTGTGCAATGGATGAAGATGAACCAGATATAGAAACCAGTGGAAGAACAAGTTCACAGAGGATTGGCCGCCAGTTTCGTACAAGAACCAGTTTAGAGTTGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

425

Amino Acids

47.7

Weight (kDa)

4.44

Isoelectric Point (pI)

56.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 283 - 333 1.7e-06 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
Acc36I ACCTGC 1 cut(s) 383
AccII CGCG 1 cut(s) 558
AciI CCGC 3 cut(s) 463, 628, 1237
AclI AACGTT 1 cut(s) 35
AclWI GGATC 1 cut(s) 1158
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 3 cut(s) 190, 422, 671
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 2 cut(s) 394, 1249
AgsI TTSAA 5 cut(s) 189, 195, 535, 715, 1011
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 1145
AluBI AGCT 6 cut(s) 50, 220, 575, 608, 665, 941
AluI AGCT 6 cut(s) 50, 220, 575, 608, 665, 941
Alw26I GTCTC 3 cut(s) 543, 637, 1078
AlwI GGATC 1 cut(s) 1158
AoxI GGCC 1 cut(s) 1234
ApeKI GCWGC 4 cut(s) 455, 572, 605, 1142
ApoI RAATTY 3 cut(s) 190, 422, 671
Asp700I GAANNNNTTC 1 cut(s) 1219
AspLEI GCGC 2 cut(s) 560, 1114
AspS9I GGNCC 4 cut(s) 99, 229, 238, 813
AsuHPI GGTGA 4 cut(s) 51, 122, 557, 857
AvaII GGWCC 4 cut(s) 99, 229, 238, 813
BanII GRGCYC 2 cut(s) 82, 155
BauI CACGAG 1 cut(s) 404
BbsI GAAGAC 3 cut(s) 78, 282, 1164
BbvI GCAGC 4 cut(s) 442, 584, 617, 1154
BccI CCATC 5 cut(s) 70, 658, 803, 895, 1064
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 316, 350
BciT130I CCWGG 1 cut(s) 1147
BcoDI GTCTC 3 cut(s) 543, 637, 1078
BfaI CTAG 4 cut(s) 47, 546, 569, 1061
BfmI CTRYAG 3 cut(s) 456, 660, 861
BfuAI ACCTGC 1 cut(s) 383
BisI GCNGC 5 cut(s) 456, 573, 606, 1143, 1237
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 5 cut(s) 457, 574, 607, 1144, 1238
Bme1390I CCNGG 1 cut(s) 1147
Bme18I GGWCC 4 cut(s) 99, 229, 238, 813
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 4 cut(s) 99, 229, 238, 813
BmiI GGNNCC 5 cut(s) 81, 101, 152, 522, 814
BmrFI CCNGG 1 cut(s) 1147
BmsI GCATC 4 cut(s) 586, 679, 988, 1039
BpiI GAAGAC 3 cut(s) 78, 282, 1164
BpmI CTGGAG 1 cut(s) 242
Bpu10I CCTNAGC 1 cut(s) 309
Bpu1102I GCTNAGC 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 1016
BsaI GGTCTC 3 cut(s) 543, 637, 1078
BsaJI CCNNGG 1 cut(s) 1146
BsaWI WCCGGW 1 cut(s) 653
Bse1I ACTGG 4 cut(s) 597, 1206, 1240, 1257
Bse3DI GCAATG 1 cut(s) 1182
BseBI CCWGG 1 cut(s) 1147
BseCI ATCGAT 1 cut(s) 1016
BseDI CCNNGG 1 cut(s) 1146
BseMI GCAATG 1 cut(s) 1182
BseMII CTCAG 1 cut(s) 768
BseNI ACTGG 4 cut(s) 597, 1206, 1240, 1257
BseRI GAGGAG 3 cut(s) 20, 305, 368
BseXI GCAGC 4 cut(s) 442, 584, 617, 1154
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 1 cut(s) 558
BshFI GGCC 1 cut(s) 1236
BshVI ATCGAT 1 cut(s) 1016
BsiSI CCGG 2 cut(s) 338, 654
BslFI GGGAC 3 cut(s) 85, 155, 662
BsmAI GTCTC 3 cut(s) 543, 637, 1078
BsmFI GGGAC 3 cut(s) 85, 155, 662
BsnI GGCC 1 cut(s) 1236
Bso31I GGTCTC 3 cut(s) 543, 637, 1078
Bsp1286I GDGCHC 2 cut(s) 82, 155
Bsp143I GATC 3 cut(s) 850, 1017, 1150
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 3 cut(s) 463, 628, 1237
BspANI GGCC 1 cut(s) 1236
BspCNI CTCAG 1 cut(s) 769
BspDI ATCGAT 1 cut(s) 1016
BspFNI CGCG 1 cut(s) 558
BspLI GGNNCC 5 cut(s) 81, 101, 152, 522, 814
BspMAI CTGCAG 2 cut(s) 460, 865
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 1158
BspQI GCTCTTC 1 cut(s) 795
BspTNI GGTCTC 3 cut(s) 543, 637, 1078
BsrDI GCAATG 1 cut(s) 1182
BsrI ACTGG 4 cut(s) 597, 1206, 1240, 1257
BssECI CCNNGG 1 cut(s) 1146
BssMI GATC 3 cut(s) 850, 1017, 1150
BssSI CACGAG 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 404
Bst2UI CCWGG 1 cut(s) 1147
Bst4CI ACNGT 2 cut(s) 392, 1163
Bst6I CTCTTC 4 cut(s) 119, 405, 710, 795
BstC8I GCNNGC 2 cut(s) 52, 951
BstDEI CTNAG 4 cut(s) 303, 309, 576, 777
BstFNI CGCG 1 cut(s) 558
BstHHI GCGC 2 cut(s) 560, 1114
BstKTI GATC 3 cut(s) 853, 1020, 1153
BstMAI GTCTC 3 cut(s) 543, 637, 1078
BstMBI GATC 3 cut(s) 850, 1017, 1150
BstMWI GCNNNNNNNGC 2 cut(s) 605, 1142
BstNI CCWGG 1 cut(s) 1147
BstSCI CCNGG 1 cut(s) 1145
BstSFI CTRYAG 3 cut(s) 456, 660, 861
BstUI CGCG 1 cut(s) 558
BstV1I GCAGC 4 cut(s) 442, 584, 617, 1154
BstV2I GAAGAC 3 cut(s) 78, 282, 1164
Bsu15I ATCGAT 1 cut(s) 1016
BsuRI GGCC 1 cut(s) 1236
BsuTUI ATCGAT 1 cut(s) 1016
BtrI CACGTC 1 cut(s) 262
BtsI GCAGTG 2 cut(s) 365, 642
BtsIMutI CAGTG 4 cut(s) 365, 642, 1168, 1213
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 52, 951
CfoI GCGC 2 cut(s) 560, 1114
Cfr13I GGNCC 4 cut(s) 99, 229, 238, 813
ClaI ATCGAT 1 cut(s) 1016
Csp6I GTAC 2 cut(s) 393, 1248
CviAII CATG 2 cut(s) 854, 928
CviQI GTAC 2 cut(s) 393, 1248
DdeI CTNAG 4 cut(s) 303, 309, 576, 777
DpnI GATC 3 cut(s) 852, 1019, 1152
DpnII GATC 3 cut(s) 850, 1017, 1150
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 4 cut(s) 119, 405, 710, 795
EarI CTCTTC 4 cut(s) 119, 405, 710, 795
Eco24I GRGCYC 2 cut(s) 82, 155
Eco31I GGTCTC 3 cut(s) 543, 637, 1078
Eco47I GGWCC 4 cut(s) 99, 229, 238, 813
Eco57I CTGAAG 1 cut(s) 705
EcoO109I RGGNCCY 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 671
EcoRII CCWGG 1 cut(s) 1145
EcoT38I GRGCYC 2 cut(s) 82, 155
FaeI CATG 2 cut(s) 857, 931
FaqI GGGAC 3 cut(s) 85, 155, 662
FatI CATG 2 cut(s) 853, 927
FauI CCCGC 1 cut(s) 621
Fnu4HI GCNGC 5 cut(s) 456, 573, 606, 1143, 1237
FokI GGATG 9 cut(s) 212, 353, 491, 608, 637, 944, 994, 1010, 1193
FriOI GRGCYC 2 cut(s) 82, 155
Fsp4HI GCNGC 5 cut(s) 456, 573, 606, 1143, 1237
FspBI CTAG 4 cut(s) 47, 546, 569, 1061
GlaI GCGC 2 cut(s) 559, 1113
GluI GCNGC 5 cut(s) 456, 573, 606, 1143, 1237
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 1 cut(s) 1236
HapII CCGG 2 cut(s) 338, 654
HhaI GCGC 2 cut(s) 560, 1114
Hin1II CATG 2 cut(s) 857, 931
Hin6I GCGC 2 cut(s) 558, 1112
HinP1I GCGC 2 cut(s) 558, 1112
HinfI GANTC 9 cut(s) 185, 506, 585, 620, 883, 914, 1076, 1106, 1122
HpaII CCGG 2 cut(s) 338, 654
HphI GGTGA 4 cut(s) 51, 122, 557, 857
Hpy166II GTNNAC 1 cut(s) 1223
Hpy188I TCNGA 3 cut(s) 495, 685, 1081
Hpy188III TCNNGA 7 cut(s) 121, 236, 398, 753, 869, 891, 1154
Hpy8I GTNNAC 1 cut(s) 1223
Hpy99I CGWCG 4 cut(s) 94, 251, 254, 257
HpyAV CCTTC 3 cut(s) 220, 242, 469
HpyCH4III ACNGT 2 cut(s) 392, 1163
HpyCH4IV ACGT 2 cut(s) 35, 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 1142
HpyF3I CTNAG 4 cut(s) 303, 309, 576, 777
HpySE526I ACGT 2 cut(s) 35, 261
Hsp92II CATG 2 cut(s) 857, 931
HspAI GCGC 2 cut(s) 558, 1112
Kzo9I GATC 3 cut(s) 850, 1017, 1150
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 4 cut(s) 85, 150, 292, 355
Lsp1109I GCAGC 4 cut(s) 442, 584, 617, 1154
LweI GCATC 4 cut(s) 586, 679, 988, 1039
MaeI CTAG 4 cut(s) 47, 546, 569, 1061
MaeII ACGT 2 cut(s) 35, 261
MaeIII GTNAC 2 cut(s) 212, 725
MalI GATC 3 cut(s) 852, 1019, 1152
MboI GATC 3 cut(s) 850, 1017, 1150
MfeI CAATTG 1 cut(s) 1131
MhlI GDGCHC 2 cut(s) 82, 155
MluCI AATT 9 cut(s) 190, 366, 422, 526, 615, 671, 708, 1065, 1131
MlyI GAGTC 4 cut(s) 579, 614, 1085, 1131
MmeI TCCRAC 3 cut(s) 613, 1059, 1248
MroXI GAANNNNTTC 1 cut(s) 1219
MseI TTAA 1 cut(s) 369
MspI CCGG 2 cut(s) 338, 654
MspR9I CCNGG 1 cut(s) 1147
MunI CAATTG 1 cut(s) 1131
MvaI CCWGG 1 cut(s) 1147
MvnI CGCG 1 cut(s) 558
MwoI GCNNNNNNNGC 2 cut(s) 605, 1142
NdeII GATC 3 cut(s) 850, 1017, 1150
NlaIII CATG 2 cut(s) 857, 931
NlaIV GGNNCC 5 cut(s) 81, 101, 152, 522, 814
NmuCI GTSAC 1 cut(s) 725
PciSI GCTCTTC 1 cut(s) 795
PcsI WCGNNNNNNNCGW 2 cut(s) 252, 258
PdmI GAANNNNTTC 1 cut(s) 1219
PfeI GAWTC 5 cut(s) 185, 506, 883, 914, 1106
PkrI GCNGC 5 cut(s) 457, 574, 607, 1144, 1238
PleI GAGTC 4 cut(s) 579, 614, 1084, 1130
PpsI GAGTC 4 cut(s) 579, 614, 1084, 1130
PpuMI RGGWCCY 1 cut(s) 229
PsiI TTATAA 1 cut(s) 348
Psp1406I AACGTT 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 1145
PspGI CCWGG 1 cut(s) 1145
PspN4I GGNNCC 5 cut(s) 81, 101, 152, 522, 814
PspPI GGNCC 4 cut(s) 99, 229, 238, 813
PspPPI RGGWCCY 1 cut(s) 229
PstI CTGCAG 2 cut(s) 460, 865
RsaI GTAC 2 cut(s) 394, 1249
RsaNI GTAC 2 cut(s) 393, 1248
SapI GCTCTTC 1 cut(s) 795
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 5 cut(s) 456, 573, 606, 1143, 1237
Sau3AI GATC 3 cut(s) 850, 1017, 1150
Sau96I GGNCC 4 cut(s) 99, 229, 238, 813
SchI GAGTC 4 cut(s) 579, 614, 1085, 1131
ScrFI CCNGG 1 cut(s) 1147
SduI GDGCHC 2 cut(s) 82, 155
SfaNI GCATC 4 cut(s) 586, 679, 988, 1039
SfcI CTRYAG 3 cut(s) 456, 660, 861
SinI GGWCC 4 cut(s) 99, 229, 238, 813
Sse9I AATT 9 cut(s) 190, 366, 422, 526, 615, 671, 708, 1065, 1131
SsiI CCGC 3 cut(s) 463, 628, 1237
SspMI CTAG 4 cut(s) 47, 546, 569, 1061
StyD4I CCNGG 1 cut(s) 1145
TaaI ACNGT 2 cut(s) 392, 1163
TaiI ACGT 2 cut(s) 38, 264
TaqI TCGA 5 cut(s) 22, 39, 120, 399, 1016
TasI AATT 9 cut(s) 190, 366, 422, 526, 615, 671, 708, 1065, 1131
TatI WGTACW 1 cut(s) 392
TauI GCSGC 1 cut(s) 1239
TfiI GAWTC 5 cut(s) 185, 506, 883, 914, 1106
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 365, 649, 1168, 1213
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 4 cut(s) 455, 572, 605, 1142
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 8 cut(s) 435, 488, 519, 684, 813, 906, 1197, 1203
TspGWI ACGGA 4 cut(s) 72, 895, 956, 1010
TspRI CASTG 4 cut(s) 365, 649, 1168, 1213
VpaK11BI GGWCC 4 cut(s) 99, 229, 238, 813
XapI RAATTY 3 cut(s) 190, 422, 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 1219
XspI CTAG 4 cut(s) 47, 546, 569, 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.