RLG00000023817

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006057
Physical Location & Seq
Forward (+)
28648589 .. 28649601
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023817

Sequence Viewer

Length: 852 bp
ATGCAAGACAGTTTCAGCTTCCGAGTAGGCAAGACCTTCGACTCTCTACCTGGTTCATGGTCCCTTGGAGATGAGAAAATCGATAGAAGGGAATGTAACAGGAACAAGGAGAGCCCTGAACCCGAATCCGAGCTCCACCAATACCACACATATTCAACGGATGACTTGGGTAACGAGCTGGAGAATGGCCTCCTAGAATTTTTGGACAAGTTCTTAGTACAGGAAGAAGAACGTGACTCTGTAGATCGTCCTGATATGAATGAGTACGTAAACGACGACGATGAGACAGACAGTGATGAGGTTCCCAATTTGCTCAAGGGTTTCTCTGGAGACCCACTTGAAATTGGCATAAAATCGCTTTTGAAGAGGACGAGTATTGATGATCAATTAGTCTCATCTACAGTAGAAAAACGTGTTCGATTTCAAGATGAAGAGGAGCATGAAGGATCAAAAGATCGTTGGTCTGCAGCGGTTCCAGATTACATACGGAATCCATCAAGATATACACATTATACGATTGATTCATCTAGTGACACGGATGACAAATCTAACGAGCAAGTCTATTTGAACTTCCGTATGCAATTTTCTAGATTGATGAAGTCAGATACTATGGAAGCCGAGGAAGTTGCTTGTATTGCCGTTTCAAAACCAGTCGCATTGGTACCAAGAAAGAAATCAACTGATGCTAGTAAGGAACTTGCGCCTTGCAGAATCAGGCCAATTGCTTTTCCAGACACTGATGAGATGGAGGAAGAAGAACCGGTGACAGGATCAAGCAGTGGAAGAGCCGGCGTGCGGAGAACTGTCCCCCGCCAGTTTCGTACAAACACCAGCTTAGAGTTGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

284

Amino Acids

32.4

Weight (kDa)

4.56

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 151 - 201 1.5e-06 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 661
AccB1I GGYRCC 1 cut(s) 661
AciI CCGC 3 cut(s) 470, 796, 811
AclWI GGATC 2 cut(s) 454, 778
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 4 cut(s) 219, 266, 663, 823
AfiI CCNNNNNNNGG 2 cut(s) 767, 795
AflIII ACRYGT 1 cut(s) 412
AgeI ACCGGT 1 cut(s) 760
AgsI TTSAA 6 cut(s) 156, 341, 364, 425, 568, 645
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 4 cut(s) 18, 133, 178, 834
AluI AGCT 4 cut(s) 18, 133, 178, 834
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 3 cut(s) 278, 324, 397
AlwI GGATC 2 cut(s) 454, 778
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 2 cut(s) 187, 716
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 197
AsiGI ACCGGT 1 cut(s) 760
Asp718I GGTACC 1 cut(s) 661
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 775
AvaII GGWCC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 661
BanII GRGCYC 2 cut(s) 116, 135
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 1 cut(s) 479
BccI CCATC 2 cut(s) 502, 739
BceAI ACGGC 1 cut(s) 623
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 608, 642
BciT130I CCWGG 1 cut(s) 51
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 3 cut(s) 278, 324, 397
BfaI CTAG 4 cut(s) 194, 528, 588, 687
BfmI CTRYAG 3 cut(s) 240, 399, 465
BisI GCNGC 1 cut(s) 468
BlsI GCNGC 1 cut(s) 469
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 4 cut(s) 62, 303, 474, 663
BmrFI CCNGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 673
BpmI CTGGAG 2 cut(s) 200, 348
BpuEI CTTGAG 1 cut(s) 299
Bsa29I ATCGAT 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 64, 618
BsaWI WCCGGW 1 cut(s) 760
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 795
Bse118I RCCGGY 2 cut(s) 760, 788
Bse1I ACTGG 2 cut(s) 650, 814
BseBI CCWGG 1 cut(s) 51
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 64, 618
BseGI GGATG 3 cut(s) 166, 544, 850
BseLI CCNNNNNNNGG 2 cut(s) 767, 795
BseNI ACTGG 2 cut(s) 650, 814
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 1 cut(s) 479
BshFI GGCC 2 cut(s) 189, 718
BshNI GGYRCC 1 cut(s) 661
BshTI ACCGGT 1 cut(s) 760
BshVI ATCGAT 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 2 cut(s) 761, 789
BslFI GGGAC 2 cut(s) 46, 791
BslI CCNNNNNNNGG 2 cut(s) 767, 795
BsmAI GTCTC 3 cut(s) 278, 324, 397
BsmFI GGGAC 2 cut(s) 46, 791
BsnI GGCC 2 cut(s) 189, 718
Bso31I GGTCTC 1 cut(s) 324
Bsp1286I GDGCHC 2 cut(s) 116, 135
Bsp143I GATC 5 cut(s) 244, 382, 446, 454, 770
BspACI CCGC 3 cut(s) 470, 796, 811
BspANI GGCC 2 cut(s) 189, 718
BspDI ATCGAT 1 cut(s) 81
BspLI GGNNCC 4 cut(s) 62, 303, 474, 663
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 2 cut(s) 454, 778
BspQI GCTCTTC 1 cut(s) 778
BspT107I GGYRCC 1 cut(s) 661
BspTNI GGTCTC 1 cut(s) 324
BsrFI RCCGGY 2 cut(s) 760, 788
BsrI ACTGG 2 cut(s) 650, 814
BssAI RCCGGY 2 cut(s) 760, 788
BssECI CCNNGG 2 cut(s) 64, 618
BssMI GATC 5 cut(s) 244, 382, 446, 454, 770
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 4 cut(s) 11, 293, 403, 805
Bst6I CTCTTC 3 cut(s) 359, 426, 778
BstBAI YACGTR 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 790, 794
BstDEI CTNAG 2 cut(s) 214, 835
BstF5I GGATG 3 cut(s) 166, 544, 850
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 5 cut(s) 247, 385, 449, 457, 773
BstMAI GTCTC 3 cut(s) 278, 324, 397
BstMBI GATC 5 cut(s) 244, 382, 446, 454, 770
BstMWI GCNNNNNNNGC 1 cut(s) 635
BstNI CCWGG 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 49
BstSFI CTRYAG 3 cut(s) 240, 399, 465
BstSNI TACGTA 1 cut(s) 268
BstV1I GCAGC 1 cut(s) 479
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 2 cut(s) 189, 718
BsuTUI ATCGAT 1 cut(s) 81
BtsCI GGATG 3 cut(s) 166, 544, 850
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 3 cut(s) 298, 735, 784
Cac8I GCNNGC 2 cut(s) 790, 794
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 1 cut(s) 703
Cfr10I RCCGGY 2 cut(s) 760, 788
Cfr13I GGNCC 1 cut(s) 60
ClaI ATCGAT 1 cut(s) 81
CsiI ACCWGGT 1 cut(s) 49
Csp6I GTAC 4 cut(s) 218, 265, 662, 822
CspAI ACCGGT 1 cut(s) 760
CviAII CATG 2 cut(s) 57, 440
CviJI RGCY 9 cut(s) 18, 114, 133, 178, 189, 617, 718, 788, 834
CviKI_1 RGCY 9 cut(s) 18, 114, 133, 178, 189, 617, 718, 788, 834
CviQI GTAC 4 cut(s) 218, 265, 662, 822
DdeI CTNAG 2 cut(s) 214, 835
DpnI GATC 5 cut(s) 246, 384, 448, 456, 772
DpnII GATC 5 cut(s) 244, 382, 446, 454, 770
Eam1104I CTCTTC 3 cut(s) 359, 426, 778
EarI CTCTTC 3 cut(s) 359, 426, 778
Ecl136II GAGCTC 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 64
Eco24I GRGCYC 2 cut(s) 116, 135
Eco31I GGTCTC 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 64
EcoT38I GRGCYC 2 cut(s) 116, 135
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 2 cut(s) 60, 443
FaqI GGGAC 2 cut(s) 46, 791
FatI CATG 2 cut(s) 56, 439
FauI CCCGC 1 cut(s) 818
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 1 cut(s) 468
FokI GGATG 2 cut(s) 173, 551
FriOI GRGCYC 2 cut(s) 116, 135
Fsp4HI GCNGC 1 cut(s) 468
FspBI CTAG 4 cut(s) 194, 528, 588, 687
GlaI GCGC 1 cut(s) 702
GluI GCNGC 1 cut(s) 468
GsuI CTGGAG 2 cut(s) 200, 348
HaeIII GGCC 2 cut(s) 189, 718
HapII CCGG 2 cut(s) 761, 789
HhaI GCGC 1 cut(s) 703
Hin1II CATG 2 cut(s) 60, 443
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HinfI GANTC 6 cut(s) 41, 125, 236, 490, 521, 711
HpaII CCGG 2 cut(s) 761, 789
HphI GGTGA 1 cut(s) 775
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 3 cut(s) 23, 130, 604
Hpy188III TCNNGA 7 cut(s) 251, 327, 425, 476, 498, 588, 731
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 2 cut(s) 278, 281
HpyAV CCTTC 3 cut(s) 46, 81, 437
HpyCH4III ACNGT 4 cut(s) 11, 293, 403, 805
HpyCH4IV ACGT 3 cut(s) 232, 267, 412
HpyCH4V TGCA 4 cut(s) 4, 467, 580, 708
HpyF10VI GCNNNNNNNGC 1 cut(s) 635
HpyF3I CTNAG 2 cut(s) 214, 835
HpySE526I ACGT 3 cut(s) 232, 267, 412
Hsp92II CATG 2 cut(s) 60, 443
HspAI GCGC 1 cut(s) 701
KpnI GGTACC 1 cut(s) 665
KroI GCCGGC 1 cut(s) 788
KroNI GCCGGC 1 cut(s) 790
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 244, 382, 446, 454, 770
LguI GCTCTTC 1 cut(s) 778
LmnI GCTCC 2 cut(s) 138, 436
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 1 cut(s) 673
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 4 cut(s) 194, 528, 588, 687
MaeII ACGT 3 cut(s) 232, 267, 412
MaeIII GTNAC 5 cut(s) 95, 170, 233, 530, 763
MalI GATC 5 cut(s) 246, 384, 448, 456, 772
MboI GATC 5 cut(s) 244, 382, 446, 454, 770
MboII GAAGA 7 cut(s) 236, 239, 376, 443, 764, 767, 795
MfeI CAATTG 1 cut(s) 720
MhlI GDGCHC 2 cut(s) 116, 135
MluCI AATT 6 cut(s) 197, 307, 342, 386, 581, 720
MlyI GAGTC 2 cut(s) 35, 230
MmeI TCCRAC 1 cut(s) 822
MnlI CCTC 6 cut(s) 200, 292, 360, 427, 613, 742
MroNI GCCGGC 1 cut(s) 788
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 761, 789
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 1 cut(s) 720
MvaI CCWGG 1 cut(s) 51
MwoI GCNNNNNNNGC 1 cut(s) 635
NaeI GCCGGC 1 cut(s) 790
NdeII GATC 5 cut(s) 244, 382, 446, 454, 770
NgoMIV GCCGGC 1 cut(s) 788
NlaIII CATG 2 cut(s) 60, 443
NlaIV GGNNCC 4 cut(s) 62, 303, 474, 663
NmeAIII GCCGAG 1 cut(s) 643
NmuCI GTSAC 3 cut(s) 233, 530, 763
PciSI GCTCTTC 1 cut(s) 778
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 790
PfeI GAWTC 4 cut(s) 125, 490, 521, 711
PinAI ACCGGT 1 cut(s) 760
PkrI GCNGC 1 cut(s) 469
PleI GAGTC 2 cut(s) 35, 230
PpsI GAGTC 2 cut(s) 35, 230
Ppu21I YACGTR 1 cut(s) 268
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 4 cut(s) 62, 303, 474, 663
PspPI GGNCC 1 cut(s) 60
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 4 cut(s) 219, 266, 663, 823
RsaNI GTAC 4 cut(s) 218, 265, 662, 822
SacI GAGCTC 1 cut(s) 135
SapI GCTCTTC 1 cut(s) 778
SatI GCNGC 1 cut(s) 468
Sau3AI GATC 5 cut(s) 244, 382, 446, 454, 770
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 2 cut(s) 35, 230
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 2 cut(s) 116, 135
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 3 cut(s) 240, 399, 465
SinI GGWCC 1 cut(s) 60
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
SnaBI TACGTA 1 cut(s) 268
Sse9I AATT 6 cut(s) 197, 307, 342, 386, 581, 720
SsiI CCGC 3 cut(s) 470, 796, 811
SspMI CTAG 4 cut(s) 194, 528, 588, 687
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 4 cut(s) 11, 293, 403, 805
TaiI ACGT 3 cut(s) 235, 270, 415
TaqI TCGA 3 cut(s) 39, 81, 418
TasI AATT 6 cut(s) 197, 307, 342, 386, 581, 720
TatI WGTACW 1 cut(s) 217
TfiI GAWTC 4 cut(s) 125, 490, 521, 711
TscAI CASTG 3 cut(s) 298, 742, 784
TseFI GTSAC 3 cut(s) 233, 530, 763
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 3 cut(s) 233, 530, 763
TspDTI ATGAA 6 cut(s) 45, 272, 444, 456, 513, 611
TspGWI ACGGA 4 cut(s) 173, 502, 551, 563
TspRI CASTG 3 cut(s) 298, 742, 784
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 197
XbaI TCTAGA 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XspI CTAG 4 cut(s) 194, 528, 588, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.