pycom17g00250

Tumour suppressing sub-chromosomal transferable candidate 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0006057
Physical Location & Seq
Reverse (-)
163735 .. 165284
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g00250.1

Sequence Viewer

Length: 1293 bp
ATGGAGGACACGTTCAACGTTCGAGTAGGCAAAATCTTCGGCTCTCTCCCCGTTTCCTCGCCATCGCCATCGCCATCGCCGTCGTCATCGAGCGGTCAACAGTCGTGTCTGAGCTCGCTATGGTCCCTAACCGACGAGGAAATCGAGAGGAGGGAATGGAACAGAGACAAGGGCAGCCCCGAACCCGAAGCCGAACCCCTACCCTTCTACTCAAAATCAAATTCAAGGTCGAAGAATGATTTCAGTGACGACCTGGAGAAGGACCTTCTAGACCTTGACGACGACGTCGAAGACGAAGGCGAGGAGGAGCAAGAGGCATCTGGTTCTTCCAGTCAGCCCGCAACTGAGGATAAGCCAGAAGGCTACAATGAAGAGGAGTGGGAAATTAAATCCTCAATCGGCAGGGACTGTACTCTCGACTTCGAGGAAGAGGAAGATGGATATGACAAAGTGGCTGTTGGCAAGGAGACTGCCGGAGATCGCTTGTATATGAGGGATGTCAACGACTATGGGATCGATATTGATACTCAAGAGGAGGTTCCTAGTTCCATTAAGGATTTCACTAGAGACCCGCGTGCTAATCACCTGGCAGCTAAGATTAGGCTCCAACAAGATGCTGAAGCAGCTCAAAAAATTGACTCCCTCAGGGTTTCTGGGAACGGCTCATCGTCTTCTATAGCTGTTGAAAACAGCTCTGAAGATGCTGCCAACCTAAAGTCGATTCTGAAGAGGAAGAATGATAATCAGATAGACTCGTCCAAAACACAAAAACGTGTCCGGTTTGATTCCAACTGCAAAAGTAACGAAGATGAAGAGGAAGCCAAAGATGTTCCTGTAGAAGCTCATTCAAATGAAAATCCTCCAGTTCCGGATTATATACGAAATCCTTCAAGATATACGCATTATACGTTTGATTCATCAGGTGATGTGGACGAGGAGTCTAACAAGCAAGCCTATATGGAATTCCTTAATCTGGTGAGAAAGTCAAATTCTACGGAACCACAGGCAGAGGATGCTTGTGTTGATCTTTCAAAACCTGTCACATTTATACCAAGAAAGAAATCAAGTGATGCTATAATGATAGAAAACGATGGGGAGTCAGAGAGACCAGGAGGTGCTCCAAGGGAAACTGCGGCTTGCAAAGTGATGCCACTTGCCATTGCAGCAGAGGATAATGAAGATGTTTGTGCAATGGAGGAAGATGAACCGGATACAGCAATGGATGGAAGAAGCAGTATACAGAGAACTGGACGCCAATACCGTACAAAAACCAGTTTGGGATTGGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

431

Amino Acids

47.81

Weight (kDa)

4.37

Isoelectric Point (pI)

70.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 228 - 335 3e-08 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AatII GACGTC 1 cut(s) 288
AccBSI CCGCTC 1 cut(s) 93
AccI GTMKAC 1 cut(s) 1237
AccII CGCG 1 cut(s) 574
AccIII TCCGGA 1 cut(s) 868
AciI CCGC 4 cut(s) 93, 339, 572, 1133
AclI AACGTT 1 cut(s) 18
AclWI GGATC 1 cut(s) 521
AcsI RAATTY 3 cut(s) 220, 962, 988
AcuI CTGAAG 3 cut(s) 639, 717, 746
AcyI GRCGYC 2 cut(s) 285, 1252
AfaI GTAC 2 cut(s) 412, 1264
AfiI CCNNNNNNNGG 2 cut(s) 259, 973
AflIII ACRYGT 2 cut(s) 9, 772
AgsI TTSAA 6 cut(s) 16, 225, 686, 849, 891, 1032
AhdI GACNNNNNGTC 1 cut(s) 937
AjnI CCWGG 3 cut(s) 252, 585, 1108
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 6 cut(s) 114, 593, 626, 680, 693, 842
AluI AGCT 6 cut(s) 114, 593, 626, 680, 693, 842
Alw21I GWGCWC 2 cut(s) 116, 1120
Alw26I GTCTC 4 cut(s) 159, 461, 561, 1099
AlwI GGATC 1 cut(s) 521
AlwNI CAGNNNCTG 1 cut(s) 408
Aor13HI TCCGGA 1 cut(s) 868
ApeKI GCWGC 5 cut(s) 174, 590, 623, 704, 1163
ApoI RAATTY 3 cut(s) 220, 962, 988
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
Asp700I GAANNNNTTC 2 cut(s) 239, 886
AspS9I GGNCC 2 cut(s) 123, 262
AsuHPI GGTGA 3 cut(s) 575, 935, 988
AvaII GGWCC 2 cut(s) 123, 262
AxyI CCTNAGG 1 cut(s) 644
BanII GRGCYC 1 cut(s) 116
BbsI GAAGAC 2 cut(s) 297, 663
Bbv12I GWGCWC 2 cut(s) 116, 1120
BbvI GCAGC 5 cut(s) 186, 602, 635, 691, 1175
BccI CCATC 6 cut(s) 70, 76, 82, 431, 1085, 1217
BceAI ACGGC 2 cut(s) 64, 676
BcgI CGANNNNNNTGC 2 cut(s) 19, 53
BciT130I CCWGG 3 cut(s) 254, 587, 1110
BciVI GTATCC 1 cut(s) 1204
BcoDI GTCTC 4 cut(s) 159, 461, 561, 1099
BfaI CTAG 3 cut(s) 269, 543, 564
BfmI CTRYAG 2 cut(s) 675, 834
BfuI GTATCC 1 cut(s) 1204
BisI GCNGC 6 cut(s) 175, 591, 624, 705, 1134, 1164
BlsI GCNGC 6 cut(s) 176, 592, 625, 706, 1135, 1165
Bme1390I CCNGG 3 cut(s) 254, 587, 1110
Bme18I GGWCC 2 cut(s) 123, 262
BmeRI GACNNNNNGTC 1 cut(s) 937
BmgT120I GGNCC 2 cut(s) 123, 262
BmiI GGNNCC 4 cut(s) 125, 540, 605, 999
BmrFI CCNGG 3 cut(s) 254, 587, 1110
BmsI GCATC 6 cut(s) 326, 604, 691, 1003, 1060, 1137
BpiI GAAGAC 2 cut(s) 297, 663
BpmI CTGGAG 2 cut(s) 275, 846
BpuEI CTTGAG 1 cut(s) 513
Bsa29I ATCGAT 1 cut(s) 516
BsaHI GRCGYC 2 cut(s) 285, 1252
BsaI GGTCTC 2 cut(s) 561, 1099
BsaJI CCNNGG 1 cut(s) 1121
BsaWI WCCGGW 3 cut(s) 777, 868, 1207
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 973
Bse1I ACTGG 4 cut(s) 330, 863, 1252, 1272
Bse21I CCTNAGG 1 cut(s) 644
Bse3DI GCAATG 3 cut(s) 1158, 1197, 1224
BseAI TCCGGA 1 cut(s) 868
BseBI CCWGG 3 cut(s) 254, 587, 1110
BseCI ATCGAT 1 cut(s) 516
BseDI CCNNGG 1 cut(s) 1121
BseGI GGATG 4 cut(s) 502, 1018, 1228, 1291
BseLI CCNNNNNNNGG 2 cut(s) 259, 973
BseMI GCAATG 3 cut(s) 1158, 1197, 1224
BseMII CTCAG 3 cut(s) 101, 336, 658
BseNI ACTGG 4 cut(s) 330, 863, 1252, 1272
BseRI GAGGAG 6 cut(s) 163, 317, 320, 389, 548, 950
BseXI GCAGC 5 cut(s) 186, 602, 635, 691, 1175
Bsh1236I CGCG 1 cut(s) 574
BshVI ATCGAT 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 116, 1120
BsiSI CCGG 4 cut(s) 474, 778, 869, 1208
BslFI GGGAC 2 cut(s) 109, 419
BslI CCNNNNNNNGG 2 cut(s) 259, 973
BsmAI GTCTC 4 cut(s) 159, 461, 561, 1099
BsmFI GGGAC 2 cut(s) 109, 419
Bso31I GGTCTC 2 cut(s) 561, 1099
Bsp1286I GDGCHC 2 cut(s) 116, 1120
Bsp13I TCCGGA 1 cut(s) 868
Bsp143I GATC 3 cut(s) 478, 513, 1024
BspACI CCGC 4 cut(s) 93, 339, 572, 1133
BspCNI CTCAG 3 cut(s) 102, 337, 657
BspDI ATCGAT 1 cut(s) 516
BspEI TCCGGA 1 cut(s) 868
BspFNI CGCG 1 cut(s) 574
BspLI GGNNCC 4 cut(s) 125, 540, 605, 999
BspPI GGATC 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 561, 1099
BsrBI CCGCTC 1 cut(s) 93
BsrDI GCAATG 3 cut(s) 1158, 1197, 1224
BsrI ACTGG 4 cut(s) 330, 863, 1252, 1272
BssECI CCNNGG 1 cut(s) 1121
BssMI GATC 3 cut(s) 478, 513, 1024
BssNAI GTATAC 1 cut(s) 1238
BssNI GRCGYC 2 cut(s) 285, 1252
BssT1I CCWWGG 1 cut(s) 1121
Bst1107I GTATAC 1 cut(s) 1238
Bst2UI CCWGG 3 cut(s) 254, 587, 1110
Bst4CI ACNGT 3 cut(s) 102, 410, 1262
Bst6I CTCTTC 4 cut(s) 366, 423, 722, 807
BstACI GRCGYC 2 cut(s) 285, 1252
BstAPI GCANNNNNTGC 1 cut(s) 1013
BstC8I GCNNGC 5 cut(s) 116, 339, 576, 951, 1138
BstDEI CTNAG 4 cut(s) 110, 345, 594, 644
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 502, 1018, 1228, 1291
BstFNI CGCG 1 cut(s) 574
BstKTI GATC 3 cut(s) 481, 516, 1027
BstMAI GTCTC 4 cut(s) 159, 461, 561, 1099
BstMBI GATC 3 cut(s) 478, 513, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 623, 1013, 1163
BstNI CCWGG 3 cut(s) 254, 587, 1110
BstSCI CCNGG 3 cut(s) 252, 585, 1108
BstSFI CTRYAG 2 cut(s) 675, 834
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 5 cut(s) 186, 602, 635, 691, 1175
BstV2I GAAGAC 2 cut(s) 297, 663
BstZ17I GTATAC 1 cut(s) 1238
Bsu15I ATCGAT 1 cut(s) 516
Bsu36I CCTNAGG 1 cut(s) 644
BsuI GTATCC 1 cut(s) 1204
BsuTUI ATCGAT 1 cut(s) 516
BtgZI GCGATG 3 cut(s) 48, 54, 60
BtsCI GGATG 4 cut(s) 502, 1018, 1228, 1291
BtsIMutI CAGTG 1 cut(s) 250
Cac8I GCNNGC 5 cut(s) 116, 339, 576, 951, 1138
CaiI CAGNNNCTG 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 123, 262
ClaI ATCGAT 1 cut(s) 516
CseI GACGC 1 cut(s) 1260
Csp6I GTAC 2 cut(s) 411, 1263
CviQI GTAC 2 cut(s) 411, 1263
DdeI CTNAG 4 cut(s) 110, 345, 594, 644
DpnI GATC 3 cut(s) 480, 515, 1026
DpnII GATC 3 cut(s) 478, 513, 1024
DrdI GACNNNNNNGTC 1 cut(s) 284
DriI GACNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 4 cut(s) 366, 423, 722, 807
Eam1105I GACNNNNNGTC 1 cut(s) 937
EarI CTCTTC 4 cut(s) 366, 423, 722, 807
Ecl136II GAGCTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 1121
Eco24I GRGCYC 1 cut(s) 116
Eco31I GGTCTC 2 cut(s) 561, 1099
Eco47I GGWCC 2 cut(s) 123, 262
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 3 cut(s) 639, 717, 746
Eco81I CCTNAGG 1 cut(s) 644
EcoICRI GAGCTC 1 cut(s) 114
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 3 cut(s) 252, 585, 1108
EcoT14I CCWWGG 1 cut(s) 1121
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 1121
FaqI GGGAC 2 cut(s) 109, 419
FauI CCCGC 2 cut(s) 346, 579
FblI GTMKAC 1 cut(s) 1237
Fnu4HI GCNGC 6 cut(s) 175, 591, 624, 705, 1134, 1164
FokI GGATG 3 cut(s) 509, 1025, 1235
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 6 cut(s) 175, 591, 624, 705, 1134, 1164
FspBI CTAG 3 cut(s) 269, 543, 564
GluI GCNGC 6 cut(s) 175, 591, 624, 705, 1134, 1164
GsuI CTGGAG 2 cut(s) 275, 846
HapII CCGG 4 cut(s) 474, 778, 869, 1208
HgaI GACGC 1 cut(s) 1260
Hin1I GRCGYC 2 cut(s) 285, 1252
HincII GTYRAC 2 cut(s) 98, 502
HindII GTYRAC 2 cut(s) 98, 502
HinfI GANTC 7 cut(s) 638, 721, 752, 785, 914, 938, 1097
HpaII CCGG 4 cut(s) 474, 778, 869, 1208
HphI GGTGA 3 cut(s) 575, 935, 988
Hpy166II GTNNAC 4 cut(s) 98, 502, 931, 1238
Hpy188I TCNGA 5 cut(s) 111, 697, 726, 747, 1102
Hpy188III TCNNGA 6 cut(s) 145, 269, 416, 530, 869, 891
Hpy8I GTNNAC 4 cut(s) 98, 502, 931, 1238
Hpy99I CGWCG 5 cut(s) 85, 137, 284, 287, 290
HpyAV CCTTC 6 cut(s) 214, 253, 275, 290, 353, 897
HpyCH4III ACNGT 3 cut(s) 102, 410, 1262
HpyCH4IV ACGT 5 cut(s) 11, 18, 285, 772, 908
HpyCH4V TGCA 4 cut(s) 795, 1140, 1163, 1190
HpyF10VI GCNNNNNNNGC 3 cut(s) 623, 1013, 1163
HpyF3I CTNAG 4 cut(s) 110, 345, 594, 644
HpySE526I ACGT 5 cut(s) 11, 18, 285, 772, 908
Hsp92I GRCGYC 2 cut(s) 285, 1252
Kpn2I TCCGGA 1 cut(s) 868
Kzo9I GATC 3 cut(s) 478, 513, 1024
LmnI GCTCC 3 cut(s) 307, 609, 1123
Lsp1109I GCAGC 5 cut(s) 186, 602, 635, 691, 1175
LweI GCATC 6 cut(s) 326, 604, 691, 1003, 1060, 1137
MaeI CTAG 3 cut(s) 269, 543, 564
MaeII ACGT 5 cut(s) 11, 18, 285, 772, 908
MaeIII GTNAC 3 cut(s) 245, 800, 1039
MalI GATC 3 cut(s) 480, 515, 1026
MbiI CCGCTC 1 cut(s) 93
MboI GATC 3 cut(s) 478, 513, 1024
MhlI GDGCHC 2 cut(s) 116, 1120
MluCI AATT 5 cut(s) 220, 384, 633, 962, 988
MlyI GAGTC 4 cut(s) 632, 746, 947, 1106
MmeI TCCRAC 2 cut(s) 631, 813
MroI TCCGGA 1 cut(s) 868
MroXI GAANNNNTTC 2 cut(s) 239, 886
MseI TTAA 3 cut(s) 387, 552, 969
MslI CAYNNNNRTG 1 cut(s) 849
MspI CCGG 4 cut(s) 474, 778, 869, 1208
MspR9I CCNGG 3 cut(s) 254, 587, 1110
MvaI CCWGG 3 cut(s) 254, 587, 1110
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 3 cut(s) 623, 1013, 1163
NdeII GATC 3 cut(s) 478, 513, 1024
NlaIV GGNNCC 4 cut(s) 125, 540, 605, 999
NmuCI GTSAC 2 cut(s) 245, 1039
PcsI WCGNNNNNNNCGW 6 cut(s) 77, 141, 285, 291, 905, 1258
PdmI GAANNNNTTC 2 cut(s) 239, 886
PfeI GAWTC 3 cut(s) 721, 785, 914
PflFI GACNNNGTC 1 cut(s) 284
PkrI GCNGC 6 cut(s) 176, 592, 625, 706, 1135, 1165
PleI GAGTC 4 cut(s) 632, 746, 946, 1105
PpsI GAGTC 4 cut(s) 632, 746, 946, 1105
PpuMI RGGWCCY 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 116
Psp1406I AACGTT 1 cut(s) 18
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 3 cut(s) 252, 585, 1108
PspGI CCWGG 3 cut(s) 252, 585, 1108
PspN4I GGNNCC 4 cut(s) 125, 540, 605, 999
PspPI GGNCC 2 cut(s) 123, 262
PspPPI RGGWCCY 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 408
PsyI GACNNNGTC 1 cut(s) 284
RsaI GTAC 2 cut(s) 412, 1264
RsaNI GTAC 2 cut(s) 411, 1263
RseI CAYNNNNRTG 1 cut(s) 849
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 3 cut(s) 387, 552, 969
SatI GCNGC 6 cut(s) 175, 591, 624, 705, 1134, 1164
Sau3AI GATC 3 cut(s) 478, 513, 1024
Sau96I GGNCC 2 cut(s) 123, 262
SchI GAGTC 4 cut(s) 632, 746, 947, 1106
ScrFI CCNGG 3 cut(s) 254, 587, 1110
SduI GDGCHC 2 cut(s) 116, 1120
SfaNI GCATC 6 cut(s) 326, 604, 691, 1003, 1060, 1137
SfcI CTRYAG 2 cut(s) 675, 834
SinI GGWCC 2 cut(s) 123, 262
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 5 cut(s) 220, 384, 633, 962, 988
SsiI CCGC 4 cut(s) 93, 339, 572, 1133
SspMI CTAG 3 cut(s) 269, 543, 564
SstI GAGCTC 1 cut(s) 116
StyD4I CCNGG 3 cut(s) 252, 585, 1108
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 3 cut(s) 102, 410, 1262
TaiI ACGT 5 cut(s) 14, 21, 288, 775, 911
TaqI TCGA 9 cut(s) 22, 89, 144, 230, 288, 417, 423, 516, 719
TasI AATT 5 cut(s) 220, 384, 633, 962, 988
TatI WGTACW 1 cut(s) 410
TauI GCSGC 1 cut(s) 1136
TfiI GAWTC 3 cut(s) 721, 785, 914
Tru1I TTAA 3 cut(s) 387, 552, 969
Tru9I TTAA 3 cut(s) 387, 552, 969
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 2 cut(s) 245, 1039
TseI GCWGC 5 cut(s) 174, 590, 623, 704, 1163
Tsp45I GTSAC 2 cut(s) 245, 1039
TspDTI ATGAA 6 cut(s) 384, 825, 867, 906, 1191, 1218
TspGWI ACGGA 1 cut(s) 1010
TspRI CASTG 1 cut(s) 250
Tth111I GACNNNGTC 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 123, 262
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 3 cut(s) 220, 962, 988
XbaI TCTAGA 1 cut(s) 268
XcmI CCANNNNNNNNNTGG 1 cut(s) 1279
XmiI GTMKAC 1 cut(s) 1237
XmnI GAANNNNTTC 2 cut(s) 239, 886
XspI CTAG 3 cut(s) 269, 543, 564
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.