FvH4_7g28941

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005924
Physical Location & Seq
Forward (+)
21382310 .. 21383073
764 bp
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UTR
Exon/CDS
Intron
FvH4_7g28941.t1

Sequence Viewer

Length: 573 bp
ATGGAGGATCCGGCCAAGAAATCGTTGTACATATGTAGCCTGGAGGCGTACCTGATCGCTAACGTGATGTCAGGCTGCTACAGTGTCCAATTGTTCTCTCCGGCGGAGTTGAACAACCAAGACGACGTGAATCGATCTTCCGACTTCTATGTGTTGGAGACCAATCCCAAGTTGCGAAGAGTTAAGAAGCTCAGCATCATCAAAGCTGAAAGAGGCAAATCCAAAGCCAAAGCTCGTGATGATTTCAGTATTCCACAATTACAATCACCAAAAGGACGTCCCAAGCTGATGGAGATCCAGGGTGAATTCTACGTTCTCGATCTATATCCTGAACGAGTCTCCTACATGTGCTTTGAGGTATTTGATACCAAAACCGGATTATGGGCGTCTCTTCCGGATCTTCCGCCCCCGATTAGGGAATCTACAGGACGTCTCTCTTGTGTAGTTGTGGGCACCAACCTCTTGGTGTCTTCCGGCATGACTCCAGTCTTCTACTGCTTTGAAGTGGCGGAGCCTACCGGACCATGGAAGAGTTTAGGCGAGCCTCTATCTCTTGGGATCAGATGTTTTTGA

Protein Analysis

191

Amino Acids

21.32

Weight (kDa)

7.5

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 81 - 181 2e-10 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 280, 433
AccB1I GGYRCC 1 cut(s) 452
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 3 cut(s) 104, 404, 509
AclWI GGATC 5 cut(s) 2, 15, 289, 405, 566
AcoI YGGCCR 1 cut(s) 12
AcsI RAATTY 1 cut(s) 305
AcyI GRCGYC 3 cut(s) 277, 386, 430
AfaI GTAC 2 cut(s) 29, 50
AfiI CCNNNNNNNGG 4 cut(s) 381, 414, 415, 525
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 2 cut(s) 112, 503
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 2 cut(s) 39, 297
AluBI AGCT 4 cut(s) 190, 206, 233, 286
AluI AGCT 4 cut(s) 190, 206, 233, 286
Alw26I GTCTC 4 cut(s) 152, 343, 393, 437
AlwI GGATC 5 cut(s) 2, 15, 289, 405, 566
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 75
ApoI RAATTY 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 521
AsuHPI GGTGA 2 cut(s) 258, 314
AvaII GGWCC 1 cut(s) 521
BaeGI GKGCMC 1 cut(s) 455
BamHI GGATCC 1 cut(s) 7
BanI GGYRCC 1 cut(s) 452
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 2 cut(s) 462, 481
BbvI GCAGC 1 cut(s) 62
BccI CCATC 1 cut(s) 283
BciT130I CCWGG 2 cut(s) 41, 299
BcoDI GTCTC 4 cut(s) 152, 343, 393, 437
BfmI CTRYAG 2 cut(s) 79, 423
BisI GCNGC 1 cut(s) 76
BlpI GCTNAGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 41, 299
Bme18I GGWCC 1 cut(s) 521
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 521
BmiI GGNNCC 3 cut(s) 9, 454, 513
BmrFI CCNGG 2 cut(s) 41, 299
BmsI GCATC 1 cut(s) 204
BoxI GACNNNNGTC 1 cut(s) 485
BpiI GAAGAC 2 cut(s) 462, 481
BpmI CTGGAG 2 cut(s) 62, 468
Bpu1102I GCTNAGC 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 133
BsaBI GATNNNNATC 2 cut(s) 293, 324
BsaHI GRCGYC 3 cut(s) 277, 386, 430
BsaI GGTCTC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 298, 524
BsaWI WCCGGW 3 cut(s) 374, 394, 518
Bsc4I CCNNNNNNNGG 4 cut(s) 381, 414, 415, 525
Bse1I ACTGG 1 cut(s) 485
Bse8I GATNNNNATC 2 cut(s) 293, 324
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 2 cut(s) 41, 299
BseCI ATCGAT 1 cut(s) 133
BseDI CCNNGG 2 cut(s) 298, 524
BseJI GATNNNNATC 2 cut(s) 293, 324
BseLI CCNNNNNNNGG 4 cut(s) 381, 414, 415, 525
BseMII CTCAG 1 cut(s) 205
BseNI ACTGG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 1 cut(s) 62
BshFI GGCC 1 cut(s) 14
BshNI GGYRCC 1 cut(s) 452
BshVI ATCGAT 1 cut(s) 133
BsiSI CCGG 6 cut(s) 11, 101, 375, 395, 474, 519
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 4 cut(s) 381, 414, 415, 525
BsmAI GTCTC 4 cut(s) 152, 343, 393, 437
BsmBI CGTCTC 2 cut(s) 393, 437
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 1 cut(s) 14
Bso31I GGTCTC 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
Bsp1720I GCTNAGC 1 cut(s) 191
Bsp19I CCATGG 1 cut(s) 524
BspACI CCGC 3 cut(s) 104, 404, 509
BspANI GGCC 1 cut(s) 14
BspCNI CTCAG 1 cut(s) 204
BspDI ATCGAT 1 cut(s) 133
BspEI TCCGGA 1 cut(s) 394
BspLI GGNNCC 3 cut(s) 9, 454, 513
BspPI GGATC 5 cut(s) 2, 15, 289, 405, 566
BspT107I GGYRCC 1 cut(s) 452
BspTNI GGTCTC 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 27
BsrI ACTGG 1 cut(s) 485
BssECI CCNNGG 2 cut(s) 298, 524
BssMI GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
BssNI GRCGYC 3 cut(s) 277, 386, 430
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 524
Bst2BI CACGAG 1 cut(s) 234
Bst2UI CCWGG 2 cut(s) 41, 299
Bst4CI ACNGT 1 cut(s) 83
Bst6I CTCTTC 3 cut(s) 172, 396, 524
BstACI GRCGYC 3 cut(s) 277, 386, 430
BstAUI TGTACA 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 542
BstDEI CTNAG 1 cut(s) 191
BstDSI CCRYGG 1 cut(s) 524
BstKTI GATC 7 cut(s) 10, 57, 137, 297, 322, 400, 561
BstMAI GTCTC 4 cut(s) 152, 343, 393, 437
BstMBI GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
BstNI CCWGG 2 cut(s) 41, 299
BstNSI RCATGY 1 cut(s) 349
BstPAI GACNNNNGTC 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 39, 297
BstSFI CTRYAG 2 cut(s) 79, 423
BstSLI GKGCMC 1 cut(s) 455
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 2 cut(s) 462, 481
BstX2I RGATCY 3 cut(s) 7, 294, 397
BstXI CCANNNNNNTGG 2 cut(s) 289, 463
BstYI RGATCY 3 cut(s) 7, 294, 397
Bsu15I ATCGAT 1 cut(s) 133
BsuRI GGCC 1 cut(s) 14
BsuTUI ATCGAT 1 cut(s) 133
BtgI CCRYGG 1 cut(s) 524
BtrI CACGTC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 521
ClaI ATCGAT 1 cut(s) 133
CseI GACGC 1 cut(s) 375
Csp6I GTAC 2 cut(s) 28, 49
CviAII CATG 3 cut(s) 346, 478, 525
CviQI GTAC 2 cut(s) 28, 49
DdeI CTNAG 1 cut(s) 191
DpnI GATC 7 cut(s) 9, 56, 136, 296, 321, 399, 560
DpnII GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
EaeI YGGCCR 1 cut(s) 12
Eam1104I CTCTTC 3 cut(s) 172, 396, 524
EarI CTCTTC 3 cut(s) 172, 396, 524
EciI GGCGGA 3 cut(s) 119, 393, 524
Eco130I CCWWGG 1 cut(s) 524
Eco31I GGTCTC 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 521
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 39, 297
EcoT14I CCWWGG 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 524
Esp3I CGTCTC 2 cut(s) 393, 437
FaeI CATG 3 cut(s) 349, 481, 528
FaiI YATR 8 cut(s) 32, 34, 150, 325, 347, 382, 479, 526
FaqI GGGAC 1 cut(s) 264
FatI CATG 3 cut(s) 345, 477, 524
FauNDI CATATG 1 cut(s) 32
Fnu4HI GCNGC 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 76
GsuI CTGGAG 2 cut(s) 62, 468
HaeIII GGCC 1 cut(s) 14
HapII CCGG 6 cut(s) 11, 101, 375, 395, 474, 519
HgaI GACGC 1 cut(s) 375
Hin1I GRCGYC 3 cut(s) 277, 386, 430
Hin1II CATG 3 cut(s) 349, 481, 528
HinfI GANTC 4 cut(s) 130, 336, 419, 481
HpaII CCGG 6 cut(s) 11, 101, 375, 395, 474, 519
HphI GGTGA 2 cut(s) 258, 314
Hpy188I TCNGA 2 cut(s) 142, 563
Hpy188III TCNNGA 4 cut(s) 236, 317, 329, 395
Hpy99I CGWCG 1 cut(s) 128
HpyCH4III ACNGT 1 cut(s) 83
HpyCH4IV ACGT 5 cut(s) 63, 126, 277, 312, 430
HpyF3I CTNAG 1 cut(s) 191
HpySE526I ACGT 5 cut(s) 63, 126, 277, 312, 430
Hsp92I GRCGYC 3 cut(s) 277, 386, 430
Hsp92II CATG 3 cut(s) 349, 481, 528
Kpn2I TCCGGA 1 cut(s) 394
Kzo9I GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 204
MaeII ACGT 5 cut(s) 63, 126, 277, 312, 430
MalI GATC 7 cut(s) 9, 56, 136, 296, 321, 399, 560
MboI GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
MboII GAAGA 7 cut(s) 129, 189, 383, 392, 462, 481, 541
MfeI CAATTG 1 cut(s) 89
MflI RGATCY 3 cut(s) 7, 294, 397
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 3 cut(s) 89, 257, 305
MlyI GAGTC 2 cut(s) 345, 475
MmeI TCCRAC 2 cut(s) 135, 165
MnlI CCTC 5 cut(s) 37, 206, 349, 470, 555
MroI TCCGGA 1 cut(s) 394
MseI TTAA 1 cut(s) 183
MspI CCGG 6 cut(s) 11, 101, 375, 395, 474, 519
MspR9I CCNGG 2 cut(s) 41, 299
MunI CAATTG 1 cut(s) 89
MvaI CCWGG 2 cut(s) 41, 299
NcoI CCATGG 1 cut(s) 524
NdeI CATATG 1 cut(s) 32
NdeII GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
NlaIII CATG 3 cut(s) 349, 481, 528
NlaIV GGNNCC 3 cut(s) 9, 454, 513
NspI RCATGY 1 cut(s) 349
PciI ACATGT 1 cut(s) 345
PfeI GAWTC 2 cut(s) 130, 419
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 2 cut(s) 344, 475
PpsI GAGTC 2 cut(s) 344, 475
PscI ACATGT 1 cut(s) 345
PshAI GACNNNNGTC 1 cut(s) 485
Psp6I CCWGG 2 cut(s) 39, 297
PspGI CCWGG 2 cut(s) 39, 297
PspN4I GGNNCC 3 cut(s) 9, 454, 513
PspPI GGNCC 1 cut(s) 521
PsuI RGATCY 3 cut(s) 7, 294, 397
RsaI GTAC 2 cut(s) 29, 50
RsaNI GTAC 2 cut(s) 28, 49
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 7 cut(s) 7, 54, 134, 294, 319, 397, 558
Sau96I GGNCC 1 cut(s) 521
SchI GAGTC 2 cut(s) 345, 475
ScrFI CCNGG 2 cut(s) 41, 299
SduI GDGCHC 1 cut(s) 455
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 2 cut(s) 79, 423
SinI GGWCC 1 cut(s) 521
Sse9I AATT 3 cut(s) 89, 257, 305
SsiI CCGC 3 cut(s) 104, 404, 509
StyD4I CCNGG 2 cut(s) 39, 297
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 1 cut(s) 83
TaiI ACGT 5 cut(s) 66, 129, 280, 315, 433
TaqI TCGA 2 cut(s) 133, 318
TasI AATT 3 cut(s) 89, 257, 305
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 2 cut(s) 130, 419
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TscAI CASTG 1 cut(s) 88
TseI GCWGC 1 cut(s) 75
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 521
XapI RAATTY 1 cut(s) 305
XceI RCATGY 1 cut(s) 349
ZraI GACGTC 2 cut(s) 278, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.