pycom05g01990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005924
Physical Location & Seq
Forward (+)
2293560 .. 2293913
354 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01990.1

Sequence Viewer

Length: 354 bp
ATGCCCCACTTGCAAAGCCAAAGCCAAGGCAGCCAAAACCCAAAAGGGCCGGAAGAAAAGCGGTGGCCGACGGTTCCAATGGTGAGTAGTTCGTGTTACGTAAAGTGGCTTCCATGCACAACGAGATCTTCACTATGGATCATGGTCTCCGTCCCAGAAAGTCCACTCTTCTGGTTCGACGTTGCTGAACCCGCTCTACCATGGAGACGGCTCCCTCCTCCTCGTCTTCCTTCCCATGGCACGGCTTTAGTCCTCATTGATGGTCAGCTAATGTCCACCTACGCTTTTGCGCCTAGTATACAAGCCTTCACTAGCTGCCTGGAACATAACTATCATTACTATGATGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.38

Weight (kDa)

6.39

Isoelectric Point (pI)

106.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 194
AccI GTMKAC 1 cut(s) 298
AciI CCGC 2 cut(s) 61, 192
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 1 cut(s) 65
AfiI CCNNNNNNNGG 2 cut(s) 236, 241
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 2 cut(s) 268, 315
AluI AGCT 2 cut(s) 268, 315
Alw26I GTCTC 2 cut(s) 151, 199
AlwI GGATC 1 cut(s) 146
AoxI GGCC 2 cut(s) 47, 65
ApeKI GCWGC 2 cut(s) 30, 315
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 1 cut(s) 94
BarI GAAGNNNNNNTAC 2 cut(s) 93, 125
BbsI GAAGAC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 42, 302
BccI CCATC 1 cut(s) 254
BceAI ACGGC 2 cut(s) 224, 258
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 151, 199
BfaI CTAG 2 cut(s) 294, 312
BglII AGATCT 1 cut(s) 125
BisI GCNGC 2 cut(s) 31, 316
BlsI GCNGC 2 cut(s) 32, 317
Bme1390I CCNGG 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 75, 212
BmrFI CCNGG 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 100
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 25, 200, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 241
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 3 cut(s) 25, 200, 235
BseLI CCNNNNNNNGG 2 cut(s) 236, 241
BseRI GAGGAG 2 cut(s) 207, 210
BseXI GCAGC 2 cut(s) 42, 302
BshFI GGCC 2 cut(s) 49, 67
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 2 cut(s) 236, 241
BsmAI GTCTC 2 cut(s) 151, 199
BsmBI CGTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 2 cut(s) 49, 67
Bso31I GGTCTC 1 cut(s) 151
Bsp143I GATC 2 cut(s) 125, 138
Bsp19I CCATGG 2 cut(s) 200, 235
BspACI CCGC 2 cut(s) 61, 192
BspANI GGCC 2 cut(s) 49, 67
BspLI GGNNCC 2 cut(s) 75, 212
BspPI GGATC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 151
BsrBI CCGCTC 1 cut(s) 194
BssECI CCNNGG 3 cut(s) 25, 200, 235
BssMI GATC 2 cut(s) 125, 138
BssNAI GTATAC 1 cut(s) 299
BssT1I CCWWGG 3 cut(s) 25, 200, 235
Bst1107I GTATAC 1 cut(s) 299
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 1 cut(s) 173
BstBAI YACGTR 1 cut(s) 100
BstDSI CCRYGG 2 cut(s) 200, 235
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 2 cut(s) 128, 141
BstMAI GTCTC 2 cut(s) 151, 199
BstMBI GATC 2 cut(s) 125, 138
BstMWI GCNNNNNNNGC 3 cut(s) 10, 30, 191
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstSNI TACGTA 1 cut(s) 100
BstV1I GCAGC 2 cut(s) 42, 302
BstV2I GAAGAC 1 cut(s) 218
BstX2I RGATCY 1 cut(s) 125
BstXI CCANNNNNNTGG 1 cut(s) 171
BstYI RGATCY 1 cut(s) 125
BstZ17I GTATAC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 49, 67
BtgI CCRYGG 2 cut(s) 200, 235
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 47
CviAII CATG 4 cut(s) 114, 142, 201, 236
DpnI GATC 2 cut(s) 127, 140
DpnII GATC 2 cut(s) 125, 138
EaeI YGGCCR 1 cut(s) 65
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco105I TACGTA 1 cut(s) 100
Eco130I CCWWGG 3 cut(s) 25, 200, 235
Eco31I GGTCTC 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 3 cut(s) 25, 200, 235
ErhI CCWWGG 3 cut(s) 25, 200, 235
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 4 cut(s) 117, 145, 204, 239
FaiI YATR 8 cut(s) 115, 136, 143, 202, 237, 299, 327, 342
FaqI GGGAC 1 cut(s) 137
FatI CATG 4 cut(s) 113, 141, 200, 235
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 1 cut(s) 298
Fnu4HI GCNGC 2 cut(s) 31, 316
Fsp4HI GCNGC 2 cut(s) 31, 316
FspBI CTAG 2 cut(s) 294, 312
GlaI GCGC 1 cut(s) 291
GluI GCNGC 2 cut(s) 31, 316
HaeIII GGCC 2 cut(s) 49, 67
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 292
Hin1II CATG 4 cut(s) 117, 145, 204, 239
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 3 cut(s) 164, 276, 299
Hpy8I GTNNAC 3 cut(s) 164, 276, 299
Hpy99I CGWCG 2 cut(s) 73, 182
HpyAV CCTTC 2 cut(s) 240, 316
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 2 cut(s) 99, 180
HpyCH4V TGCA 2 cut(s) 13, 117
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 30, 191
HpySE526I ACGT 2 cut(s) 99, 180
Hsp92II CATG 4 cut(s) 117, 145, 204, 239
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 2 cut(s) 125, 138
LmnI GCTCC 1 cut(s) 216
LpnPI CCDG 5 cut(s) 63, 157, 168, 305, 332
Lsp1109I GCAGC 2 cut(s) 42, 302
MaeI CTAG 2 cut(s) 294, 312
MaeII ACGT 2 cut(s) 99, 180
MaeIII GTNAC 1 cut(s) 95
MalI GATC 2 cut(s) 127, 140
MbiI CCGCTC 1 cut(s) 194
MboI GATC 2 cut(s) 125, 138
MboII GAAGA 4 cut(s) 65, 120, 160, 218
MflI RGATCY 1 cut(s) 125
MnlI CCTC 4 cut(s) 225, 228, 231, 263
MseI TTAA 1 cut(s) 352
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 10, 30, 191
NcoI CCATGG 2 cut(s) 200, 235
NdeII GATC 2 cut(s) 125, 138
NlaIII CATG 4 cut(s) 117, 145, 204, 239
NlaIV GGNNCC 2 cut(s) 75, 212
PkrI GCNGC 2 cut(s) 32, 317
Ppu21I YACGTR 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 75, 212
PspPI GGNCC 1 cut(s) 47
PsrI GAACNNNNNNTAC 2 cut(s) 180, 212
PsuI RGATCY 1 cut(s) 125
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 1 cut(s) 352
SatI GCNGC 2 cut(s) 31, 316
Sau3AI GATC 2 cut(s) 125, 138
Sau96I GGNCC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 320
SetI ASST 5 cut(s) 102, 183, 270, 281, 317
SmiMI CAYNNNNRTG 1 cut(s) 339
SnaBI TACGTA 1 cut(s) 100
SsiI CCGC 2 cut(s) 61, 192
SspMI CTAG 2 cut(s) 294, 312
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 3 cut(s) 25, 200, 235
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 2 cut(s) 102, 183
TaqI TCGA 1 cut(s) 177
Tru1I TTAA 1 cut(s) 352
Tru9I TTAA 1 cut(s) 352
TseI GCWGC 2 cut(s) 30, 315
TspGWI ACGGA 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 298
XspI CTAG 2 cut(s) 294, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.