Rh1AG409800

source UniProtKB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005924
Physical Location & Seq
Reverse (-)
63926656 .. 63951689
25034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG409800.1

Sequence Viewer

Length: 927 bp
ATGGTAGATCCTTCTCTACCAAAACCGTGGGTTGGAGCAGCTTTGAATACCACTGGTCTGAGGAAGCTTTCCCGTGTGTACAGACTTGTGCGGGTTAGCTGTGCTCCCACTCCATCACAGTCACCGTATCTGAGCAACGACTCGTCCAGTGCAAATTCTGCGGATGCAACTTTTCCCATCAACAATTGTATGCTCACTTCGGAAGCAACAGTTTGTTTTGCTTCACAATTTGTCAACTTACAACGACATGGGACGACTTCCAAGGTCTACGTGTTGGACACCTTGCGAGATAAGCCCAAGTTCGTCAAACGCAAAGCCAACAAACCCAAAGCTTGTGATCATGGAATTCCGGAATTTCAATCACCAAAATGTCTTCCCCAGCTGATGGAGACCGACGGTAAACTGTACACTCTCGACTTAAAGCCTCAACAAGGCTTCCCCAGCTCCTCGTCCTTTGAGGTATTTGACCCCCAAACCAGATCGTGGGCCGCTCTTCCGGATCATCCTCCCCTGATTATCCAATGTCGTGGTCCCCTCTCTTGTGCAGTGGGCACCAAATTCTTGATCTCTGCCTGCGATACTCCGGTCTACTGCTTTGACGTGGCGGAGCCTACACGACCATGGAATGAACTCTGGAAACCTATCAAGGATAATGCTGAGAAGAAAAGGAAAAAGAAAGAGACAGCGGATGAAGACTCAGAGGTACAATTTAGCTTGGTATCAATGGCACCTGCATTACCAGAATCATTGAAGATGCTATGTCAGTGGGGTGAAGACAAATTCAGAGACGGGCGTACCATCAGCTTCCACATTGAACCTGAAGTCTTCGGATATTCTCGCAAGACCTTTCTAATGGGATCAGATGTTCGGCGACTTGCAAGCATGAGGGAACTTAGTGGAACTTGCATTGCTGTGTACCAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 Transposase_24

Protein Analysis

308

Amino Acids

34.48

Weight (kDa)

8.89

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 115 - 210 2.6e-06 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 739
Acc36I ACCTGC 1 cut(s) 739
AccB1I GGYRCC 2 cut(s) 551, 727
AccB7I CCANNNNNTGG 2 cut(s) 385, 483
AccBSI CCGCTC 1 cut(s) 491
AccI GTMKAC 2 cut(s) 267, 588
AccIII TCCGGA 2 cut(s) 349, 496
AciI CCGC 5 cut(s) 91, 161, 489, 605, 686
AclWI GGATC 3 cut(s) 2, 507, 865
AcsI RAATTY 5 cut(s) 154, 345, 353, 557, 779
AcuI CTGAAG 1 cut(s) 840
AfaI GTAC 5 cut(s) 80, 407, 705, 796, 917
AfiI CCNNNNNNNGG 4 cut(s) 32, 385, 431, 483
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 4 cut(s) 46, 359, 751, 815
AjiI CACGTC 1 cut(s) 601
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
AluBI AGCT 8 cut(s) 41, 67, 99, 332, 382, 444, 714, 804
AluI AGCT 8 cut(s) 41, 67, 99, 332, 382, 444, 714, 804
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 3 cut(s) 383, 674, 780
AlwI GGATC 3 cut(s) 2, 507, 865
Aor13HI TCCGGA 2 cut(s) 349, 496
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 38
ApoI RAATTY 5 cut(s) 154, 345, 353, 557, 779
AspS9I GGNCC 2 cut(s) 486, 530
AsuHPI GGTGA 3 cut(s) 114, 354, 782
AvaII GGWCC 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 554
BaeI ACNNNNGTAYC 4 cut(s) 570, 603, 778, 811
BanI GGYRCC 2 cut(s) 551, 727
BarI GAAGNNNNNNTAC 2 cut(s) 181, 213
BbsI GAAGAC 4 cut(s) 365, 699, 780, 817
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 1 cut(s) 50
BccI CCATC 4 cut(s) 121, 185, 379, 806
BclI TGATCA 1 cut(s) 337
BcoDI GTCTC 3 cut(s) 383, 674, 780
BfuAI ACCTGC 1 cut(s) 739
BisI GCNGC 2 cut(s) 39, 489
BlsI GCNGC 2 cut(s) 40, 490
Bme18I GGWCC 1 cut(s) 530
BmgBI CACGTC 1 cut(s) 601
BmgT120I GGNCC 2 cut(s) 486, 530
BmiI GGNNCC 4 cut(s) 532, 553, 609, 729
BmsI GCATC 2 cut(s) 154, 744
BpiI GAAGAC 4 cut(s) 365, 699, 780, 817
BsaAI YACGTR 1 cut(s) 271
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 26, 261, 620
BsaWI WCCGGW 3 cut(s) 349, 496, 583
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 385, 431, 483
Bse1I ACTGG 2 cut(s) 58, 147
Bse3DI GCAATG 1 cut(s) 906
BseAI TCCGGA 2 cut(s) 349, 496
BseDI CCNNGG 3 cut(s) 26, 261, 620
BseGI GGATG 3 cut(s) 169, 502, 694
BseLI CCNNNNNNNGG 4 cut(s) 32, 385, 431, 483
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 4 cut(s) 50, 122, 648, 711
BseNI ACTGG 2 cut(s) 58, 147
BseRI GAGGAG 1 cut(s) 436
BseSI GKGCMC 1 cut(s) 554
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 2 cut(s) 378, 440
BsgI GTGCAG 1 cut(s) 564
BshFI GGCC 1 cut(s) 488
BshNI GGYRCC 2 cut(s) 551, 727
BsiHKAI GWGCWC 1 cut(s) 106
BsiSI CCGG 3 cut(s) 350, 497, 584
BslFI GGGAC 2 cut(s) 265, 516
BslI CCNNNNNNNGG 4 cut(s) 32, 385, 431, 483
BsmAI GTCTC 3 cut(s) 383, 674, 780
BsmBI CGTCTC 1 cut(s) 780
BsmFI GGGAC 2 cut(s) 265, 516
BsnI GGCC 1 cut(s) 488
Bso31I GGTCTC 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 106, 554
Bsp13I TCCGGA 2 cut(s) 349, 496
Bsp1407I TGTACA 2 cut(s) 78, 405
Bsp143I GATC 6 cut(s) 7, 337, 479, 499, 564, 857
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 5 cut(s) 91, 161, 489, 605, 686
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 4 cut(s) 51, 123, 649, 710
BspEI TCCGGA 2 cut(s) 349, 496
BspLI GGNNCC 4 cut(s) 532, 553, 609, 729
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 3 cut(s) 2, 507, 865
BspQI GCTCTTC 1 cut(s) 498
BspT107I GGYRCC 2 cut(s) 551, 727
BspTNI GGTCTC 1 cut(s) 383
BsrBI CCGCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 906
BsrGI TGTACA 2 cut(s) 78, 405
BsrI ACTGG 2 cut(s) 58, 147
BssECI CCNNGG 3 cut(s) 26, 261, 620
BssMI GATC 6 cut(s) 7, 337, 479, 499, 564, 857
BssT1I CCWWGG 2 cut(s) 261, 620
Bst4CI ACNGT 6 cut(s) 27, 120, 126, 211, 398, 405
Bst6I CTCTTC 1 cut(s) 498
BstAPI GCANNNNNTGC 1 cut(s) 158
BstAUI TGTACA 2 cut(s) 78, 405
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 2 cut(s) 574, 880
BstDEI CTNAG 5 cut(s) 59, 131, 657, 697, 893
BstDSI CCRYGG 2 cut(s) 26, 620
BstENI CCTNNNNNAGG 1 cut(s) 429
BstF5I GGATG 3 cut(s) 169, 502, 694
BstKTI GATC 6 cut(s) 10, 340, 482, 502, 567, 860
BstMAI GTCTC 3 cut(s) 383, 674, 780
BstMBI GATC 6 cut(s) 7, 337, 479, 499, 564, 857
BstMWI GCNNNNNNNGC 3 cut(s) 158, 292, 441
BstSLI GKGCMC 1 cut(s) 554
BstV1I GCAGC 1 cut(s) 50
BstV2I GAAGAC 4 cut(s) 365, 699, 780, 817
BstX2I RGATCY 1 cut(s) 7
BstXI CCANNNNNNTGG 2 cut(s) 27, 527
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 488
BtgI CCRYGG 2 cut(s) 26, 620
BtrI CACGTC 1 cut(s) 601
BtsCI GGATG 3 cut(s) 169, 502, 694
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 51, 154, 552, 770
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 2 cut(s) 574, 880
Cfr13I GGNCC 2 cut(s) 486, 530
Csp6I GTAC 5 cut(s) 79, 406, 704, 795, 916
CviAII CATG 4 cut(s) 248, 341, 621, 883
CviQI GTAC 5 cut(s) 79, 406, 704, 795, 916
DdeI CTNAG 5 cut(s) 59, 131, 657, 697, 893
DpnI GATC 6 cut(s) 9, 339, 481, 501, 566, 859
DpnII GATC 6 cut(s) 7, 337, 479, 499, 564, 857
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 620
Eco130I CCWWGG 2 cut(s) 261, 620
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 840
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 345
EcoT14I CCWWGG 2 cut(s) 261, 620
ErhI CCWWGG 2 cut(s) 261, 620
Esp3I CGTCTC 1 cut(s) 780
FaeI CATG 4 cut(s) 251, 344, 624, 886
FaiI YATR 6 cut(s) 191, 249, 342, 622, 760, 884
FaqI GGGAC 2 cut(s) 265, 516
FatI CATG 4 cut(s) 247, 340, 620, 882
FauI CCCGC 1 cut(s) 84
FbaI TGATCA 1 cut(s) 337
FblI GTMKAC 2 cut(s) 267, 588
Fnu4HI GCNGC 2 cut(s) 39, 489
FokI GGATG 3 cut(s) 176, 489, 701
Fsp4HI GCNGC 2 cut(s) 39, 489
GluI GCNGC 2 cut(s) 39, 489
GsaI CCCAGC 2 cut(s) 382, 444
HaeIII GGCC 1 cut(s) 488
HapII CCGG 3 cut(s) 350, 497, 584
Hin1II CATG 4 cut(s) 251, 344, 624, 886
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 2 cut(s) 65, 330
HinfI GANTC 3 cut(s) 140, 695, 743
HpaII CCGG 3 cut(s) 350, 497, 584
HphI GGTGA 3 cut(s) 114, 354, 782
Hpy166II GTNNAC 7 cut(s) 79, 235, 268, 401, 408, 589, 916
Hpy188I TCNGA 7 cut(s) 60, 132, 202, 700, 785, 830, 862
Hpy188III TCNNGA 5 cut(s) 350, 413, 497, 562, 634
Hpy8I GTNNAC 7 cut(s) 79, 235, 268, 401, 408, 589, 916
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 6 cut(s) 27, 120, 126, 211, 398, 405
HpyCH4IV ACGT 2 cut(s) 270, 600
HpyCH4V TGCA 6 cut(s) 152, 167, 545, 734, 878, 906
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 292, 441
HpyF3I CTNAG 5 cut(s) 59, 131, 657, 697, 893
HpySE526I ACGT 2 cut(s) 270, 600
Hsp92II CATG 4 cut(s) 251, 344, 624, 886
Kpn2I TCCGGA 2 cut(s) 349, 496
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 6 cut(s) 7, 337, 479, 499, 564, 857
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 4 cut(s) 35, 109, 449, 607
Lsp1109I GCAGC 1 cut(s) 50
LweI GCATC 2 cut(s) 154, 744
MaeII ACGT 2 cut(s) 270, 600
MaeIII GTNAC 1 cut(s) 120
MalI GATC 6 cut(s) 9, 339, 481, 501, 566, 859
MbiI CCGCTC 1 cut(s) 491
MboI GATC 6 cut(s) 7, 337, 479, 499, 564, 857
MboII GAAGA 7 cut(s) 365, 485, 673, 704, 763, 785, 817
MfeI CAATTG 1 cut(s) 184
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 106, 554
MluCI AATT 8 cut(s) 154, 184, 227, 345, 353, 557, 707, 779
MlyI GAGTC 2 cut(s) 134, 689
MmeI TCCRAC 2 cut(s) 13, 255
MnlI CCTC 8 cut(s) 54, 435, 451, 457, 516, 545, 694, 879
MroI TCCGGA 2 cut(s) 349, 496
MseI TTAA 1 cut(s) 419
MslI CAYNNNNRTG 3 cut(s) 367, 619, 911
MspA1I CMGCKG 2 cut(s) 382, 686
MspI CCGG 3 cut(s) 350, 497, 584
MunI CAATTG 1 cut(s) 184
MwoI GCNNNNNNNGC 3 cut(s) 158, 292, 441
NcoI CCATGG 1 cut(s) 620
NdeII GATC 6 cut(s) 7, 337, 479, 499, 564, 857
NlaIII CATG 4 cut(s) 251, 344, 624, 886
NlaIV GGNNCC 4 cut(s) 532, 553, 609, 729
NmuCI GTSAC 1 cut(s) 120
PaqCI CACCTGC 1 cut(s) 739
PciSI GCTCTTC 1 cut(s) 498
PfeI GAWTC 1 cut(s) 743
PflMI CCANNNNNTGG 2 cut(s) 385, 483
PkrI GCNGC 2 cut(s) 40, 490
PleI GAGTC 2 cut(s) 134, 689
PpsI GAGTC 2 cut(s) 134, 689
Ppu21I YACGTR 1 cut(s) 271
PspFI CCCAGC 2 cut(s) 378, 440
PspN4I GGNNCC 4 cut(s) 532, 553, 609, 729
PspPI GGNCC 2 cut(s) 486, 530
PsuI RGATCY 1 cut(s) 7
PvuII CAGCTG 1 cut(s) 382
RsaI GTAC 5 cut(s) 80, 407, 705, 796, 917
RsaNI GTAC 5 cut(s) 79, 406, 704, 795, 916
RseI CAYNNNNRTG 3 cut(s) 367, 619, 911
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 2 cut(s) 39, 489
Sau3AI GATC 6 cut(s) 7, 337, 479, 499, 564, 857
Sau96I GGNCC 2 cut(s) 486, 530
SchI GAGTC 2 cut(s) 134, 689
SduI GDGCHC 2 cut(s) 106, 554
SfaNI GCATC 2 cut(s) 154, 744
SinI GGWCC 1 cut(s) 530
SmiMI CAYNNNNRTG 3 cut(s) 367, 619, 911
Sse9I AATT 8 cut(s) 154, 184, 227, 345, 353, 557, 707, 779
SsiI CCGC 5 cut(s) 91, 161, 489, 605, 686
StyI CCWWGG 2 cut(s) 261, 620
TaaI ACNGT 6 cut(s) 27, 120, 126, 211, 398, 405
TaiI ACGT 2 cut(s) 273, 603
TaqI TCGA 1 cut(s) 414
TaqII GACCGA 1 cut(s) 407
TasI AATT 8 cut(s) 154, 184, 227, 345, 353, 557, 707, 779
TatI WGTACW 2 cut(s) 78, 405
TauI GCSGC 1 cut(s) 491
TfiI GAWTC 1 cut(s) 743
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 4 cut(s) 58, 154, 552, 770
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 642, 705
TspRI CASTG 4 cut(s) 58, 154, 552, 770
Van91I CCANNNNNTGG 2 cut(s) 385, 483
VpaK11BI GGWCC 1 cut(s) 530
XagI CCTNNNNNAGG 1 cut(s) 429
XapI RAATTY 5 cut(s) 154, 345, 353, 557, 779
XmiI GTMKAC 2 cut(s) 267, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.