Rroxscaffold_4G00282050

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005924
Physical Location & Seq
Reverse (-)
4192227 .. 4193595
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282050.1

Sequence Viewer

Length: 894 bp
ATGGAGCGGAAATCGAAGTCGAAGTCGAGATTTAGGGAGGCTGTTCATCTGGATAGGGGCGACAAGGAAGCCAAGGAGTCGCTGTACATGTGTAGCATGGAGAGATATAGGGACGAGAGAGTGTTTACACATTGTTACAGTATCCAGGCCATCAACTTGACCGACTTGCTCTCCTTTTCCGATACTCCGATTACCATCTCTTTCTCCAGATCTCCACCAACCAAAGCTATGGGATCTCCACCAACCAAAGTCCAAGACCATATCCTGCGAGAAGTTGATTTTCTTCAAGGCAACGACGTTCCCTACGACATGGGTTTCGGTGTGTTTGGATCTCAGATTGTTTTGGCCGGCGGACTTCAACTTCCACAACGACATCGGACTTCCAAGGTCTACGTGTTGGACACCTTGCGAGATAAGCCCAAGTTCGTCAAACGCAAAGCCAACAAGCCCAAAGCTCGTGATCATGGAATTCCGGAATTTCAATCACCAAAACGTCTTCCCCAGCTGATGGAGATCGAGGGTAAACTGTACGCTCTCGACTTACGGCCTCAACAAGGCTTCCCCAGCTCCTCGTCCTTTGAGGTATTTGACCCCCAAACCGGATCATGGGCCGCTCTTCCGGATCATCCTCCCCCGATTATCCAATGTCGTGGTCCCCTCTCTTGTGCAGTTGTGGGCACCAAATTCTTGATCTCTGCCTGCGATACTCCGGTCTACTGCTTTGACGTGGCGGAGCCTACCCGACCATGGAATGAACTCTGGGTTCGTCATGACCGCCATAAACTGTACAATTATATTGACCCCAGAGCCCTCCCCTTTCTGCAGGTTTTGGTTTTGGACTTGGAGGAGGAAAATCATAAGATATTATTTTCCTCCTGTGGAACGATATACTAG

Protein Analysis

297

Amino Acids

33.96

Weight (kDa)

8.59

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 157 - 251 1.7e-10 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 814
AccB1I GGYRCC 1 cut(s) 677
AccB7I CCANNNNNTGG 1 cut(s) 508
AccBSI CCGCTC 2 cut(s) 7, 614
AccI GTMKAC 2 cut(s) 390, 714
AccIII TCCGGA 2 cut(s) 472, 619
AciI CCGC 5 cut(s) 7, 351, 612, 731, 775
AclWI GGATC 4 cut(s) 241, 337, 610, 630
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 3 cut(s) 468, 476, 683
AfaI GTAC 3 cut(s) 86, 530, 788
AfiI CCNNNNNNNGG 5 cut(s) 508, 554, 599, 606, 747
AflIII ACRYGT 2 cut(s) 87, 393
AgsI TTSAA 3 cut(s) 287, 359, 482
AjiI CACGTC 1 cut(s) 727
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 4 cut(s) 227, 455, 505, 567
AluI AGCT 4 cut(s) 227, 455, 505, 567
AlwI GGATC 4 cut(s) 241, 337, 610, 630
Aor13HI TCCGGA 2 cut(s) 472, 619
AoxI GGCC 4 cut(s) 147, 345, 545, 609
ApoI RAATTY 3 cut(s) 468, 476, 683
ArsI GACNNNNNNTTYG 4 cut(s) 8, 40, 299, 331
AspS9I GGNCC 2 cut(s) 609, 653
AsuHPI GGTGA 1 cut(s) 477
AvaII GGWCC 1 cut(s) 653
BaeGI GKGCMC 1 cut(s) 680
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BanI GGYRCC 1 cut(s) 677
BanII GRGCYC 1 cut(s) 811
BauI CACGAG 1 cut(s) 456
BbsI GAAGAC 1 cut(s) 488
BccI CCATC 3 cut(s) 158, 203, 502
BceAI ACGGC 1 cut(s) 560
BciT130I CCWGG 1 cut(s) 146
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 1 cut(s) 460
BfaI CTAG 1 cut(s) 892
BfmI CTRYAG 1 cut(s) 821
BfuAI ACCTGC 1 cut(s) 814
BfuI GTATCC 1 cut(s) 152
BglII AGATCT 1 cut(s) 209
BisI GCNGC 1 cut(s) 612
BlsI GCNGC 1 cut(s) 613
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 653
BmgBI CACGTC 1 cut(s) 727
BmgT120I GGNCC 2 cut(s) 609, 653
BmiI GGNNCC 3 cut(s) 655, 679, 735
BmrFI CCNGG 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 190
BsaAI YACGTR 1 cut(s) 394
BsaBI GATNNNNATC 2 cut(s) 194, 512
BsaJI CCNNGG 3 cut(s) 72, 384, 746
BsaWI WCCGGW 4 cut(s) 472, 599, 619, 709
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 155, 185
Bsc4I CCNNNNNNNGG 5 cut(s) 508, 554, 599, 606, 747
Bse118I RCCGGY 1 cut(s) 347
Bse8I GATNNNNATC 2 cut(s) 194, 512
BseAI TCCGGA 2 cut(s) 472, 619
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 3 cut(s) 72, 384, 746
BseGI GGATG 1 cut(s) 625
BseJI GATNNNNATC 2 cut(s) 194, 512
BseLI CCNNNNNNNGG 5 cut(s) 508, 554, 599, 606, 747
BseMII CTCAG 1 cut(s) 347
BseRI GAGGAG 2 cut(s) 559, 860
BseSI GKGCMC 1 cut(s) 680
BseYI CCCAGC 2 cut(s) 501, 563
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 4 cut(s) 149, 347, 547, 611
BshNI GGYRCC 1 cut(s) 677
BsiSI CCGG 5 cut(s) 348, 473, 600, 620, 710
BslFI GGGAC 2 cut(s) 125, 639
BslI CCNNNNNNNGG 5 cut(s) 508, 554, 599, 606, 747
BsmFI GGGAC 2 cut(s) 125, 639
BsnI GGCC 4 cut(s) 149, 347, 547, 611
Bsp1286I GDGCHC 2 cut(s) 680, 811
Bsp13I TCCGGA 2 cut(s) 472, 619
Bsp1407I TGTACA 2 cut(s) 84, 786
Bsp143I GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
Bsp19I CCATGG 1 cut(s) 746
BspACI CCGC 5 cut(s) 7, 351, 612, 731, 775
BspANI GGCC 4 cut(s) 149, 347, 547, 611
BspCNI CTCAG 1 cut(s) 346
BspEI TCCGGA 2 cut(s) 472, 619
BspHI TCATGA 1 cut(s) 769
BspLI GGNNCC 3 cut(s) 655, 679, 735
BspMAI CTGCAG 1 cut(s) 825
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 4 cut(s) 241, 337, 610, 630
BspQI GCTCTTC 1 cut(s) 621
BspT107I GGYRCC 1 cut(s) 677
BsrBI CCGCTC 2 cut(s) 7, 614
BsrFI RCCGGY 1 cut(s) 347
BsrGI TGTACA 2 cut(s) 84, 786
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 3 cut(s) 72, 384, 746
BssMI GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 3 cut(s) 72, 384, 746
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 140, 528, 786
Bst6I CTCTTC 1 cut(s) 621
BstAUI TGTACA 2 cut(s) 84, 786
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 2 cut(s) 349, 700
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 1 cut(s) 746
BstENI CCTNNNNNAGG 1 cut(s) 552
BstF5I GGATG 1 cut(s) 625
BstKTI GATC 8 cut(s) 212, 236, 332, 463, 516, 605, 625, 693
BstMBI GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
BstMWI GCNNNNNNNGC 2 cut(s) 415, 564
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 144
BstSFI CTRYAG 1 cut(s) 821
BstSLI GKGCMC 1 cut(s) 680
BstV2I GAAGAC 1 cut(s) 488
BstX2I RGATCY 3 cut(s) 209, 233, 329
BstXI CCANNNNNNTGG 2 cut(s) 229, 650
BstYI RGATCY 3 cut(s) 209, 233, 329
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 4 cut(s) 149, 347, 547, 611
BtgI CCRYGG 1 cut(s) 746
BtrI CACGTC 1 cut(s) 727
BtsCI GGATG 1 cut(s) 625
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 2 cut(s) 349, 700
CciI TCATGA 1 cut(s) 769
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 609, 653
Csp6I GTAC 3 cut(s) 85, 529, 787
CviAII CATG 7 cut(s) 88, 97, 310, 464, 606, 747, 770
CviQI GTAC 3 cut(s) 85, 529, 787
DdeI CTNAG 1 cut(s) 333
DpnI GATC 8 cut(s) 211, 235, 331, 462, 515, 604, 624, 692
DpnII GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
EciI GGCGGA 2 cut(s) 366, 746
Eco130I CCWWGG 3 cut(s) 72, 384, 746
Eco24I GRGCYC 1 cut(s) 811
Eco47I GGWCC 1 cut(s) 653
EcoNI CCTNNNNNAGG 1 cut(s) 552
EcoRI GAATTC 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 3 cut(s) 72, 384, 746
EcoT38I GRGCYC 1 cut(s) 811
ErhI CCWWGG 3 cut(s) 72, 384, 746
FaeI CATG 7 cut(s) 91, 100, 313, 467, 609, 750, 773
FaqI GGGAC 2 cut(s) 125, 639
FatI CATG 7 cut(s) 87, 96, 309, 463, 605, 746, 769
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 2 cut(s) 390, 714
Fnu4HI GCNGC 1 cut(s) 612
FokI GGATG 1 cut(s) 612
FriOI GRGCYC 1 cut(s) 811
Fsp4HI GCNGC 1 cut(s) 612
FspBI CTAG 1 cut(s) 892
GluI GCNGC 1 cut(s) 612
GsaI CCCAGC 2 cut(s) 505, 567
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 4 cut(s) 149, 347, 547, 611
HapII CCGG 5 cut(s) 348, 473, 600, 620, 710
Hin1II CATG 7 cut(s) 91, 100, 313, 467, 609, 750, 773
HinfI GANTC 1 cut(s) 77
HpaII CCGG 5 cut(s) 348, 473, 600, 620, 710
HphI GGTGA 1 cut(s) 477
Hpy166II GTNNAC 4 cut(s) 126, 391, 524, 715
Hpy188I TCNGA 4 cut(s) 181, 189, 336, 378
Hpy188III TCNNGA 9 cut(s) 27, 50, 207, 458, 473, 536, 620, 688, 770
Hpy8I GTNNAC 4 cut(s) 126, 391, 524, 715
Hpy99I CGWCG 1 cut(s) 299
HpyCH4III ACNGT 3 cut(s) 140, 528, 786
HpyCH4IV ACGT 4 cut(s) 297, 393, 493, 726
HpyCH4V TGCA 2 cut(s) 668, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 415, 564
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 4 cut(s) 297, 393, 493, 726
Hsp92II CATG 7 cut(s) 91, 100, 313, 467, 609, 750, 773
Kpn2I TCCGGA 2 cut(s) 472, 619
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
LguI GCTCTTC 1 cut(s) 621
LmnI GCTCC 3 cut(s) 4, 572, 733
MaeI CTAG 1 cut(s) 892
MaeII ACGT 4 cut(s) 297, 393, 493, 726
MaeIII GTNAC 1 cut(s) 134
MalI GATC 8 cut(s) 211, 235, 331, 462, 515, 604, 624, 692
MbiI CCGCTC 2 cut(s) 7, 614
MboI GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
MboII GAAGA 3 cut(s) 275, 488, 608
MflI RGATCY 3 cut(s) 209, 233, 329
MhlI GDGCHC 2 cut(s) 680, 811
MluCI AATT 4 cut(s) 468, 476, 683, 790
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 378
MroI TCCGGA 2 cut(s) 472, 619
MroNI GCCGGC 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 5 cut(s) 348, 473, 600, 620, 710
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 2 cut(s) 415, 564
NaeI GCCGGC 1 cut(s) 349
NcoI CCATGG 1 cut(s) 746
NdeII GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 7 cut(s) 91, 100, 313, 467, 609, 750, 773
NlaIV GGNNCC 3 cut(s) 655, 679, 735
NspI RCATGY 1 cut(s) 91
PagI TCATGA 1 cut(s) 769
PciI ACATGT 1 cut(s) 87
PciSI GCTCTTC 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 349
PflMI CCANNNNNTGG 1 cut(s) 508
PkrI GCNGC 1 cut(s) 613
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Ppu21I YACGTR 1 cut(s) 394
PscI ACATGT 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 144
PspFI CCCAGC 2 cut(s) 501, 563
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 655, 679, 735
PspPI GGNCC 2 cut(s) 609, 653
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 3 cut(s) 209, 233, 329
PvuII CAGCTG 1 cut(s) 505
RsaI GTAC 3 cut(s) 86, 530, 788
RsaNI GTAC 3 cut(s) 85, 529, 787
SapI GCTCTTC 1 cut(s) 621
SatI GCNGC 1 cut(s) 612
Sau3AI GATC 8 cut(s) 209, 233, 329, 460, 513, 602, 622, 690
Sau96I GGNCC 2 cut(s) 609, 653
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 2 cut(s) 680, 811
SfcI CTRYAG 1 cut(s) 821
SinI GGWCC 1 cut(s) 653
Sse9I AATT 4 cut(s) 468, 476, 683, 790
SsiI CCGC 5 cut(s) 7, 351, 612, 731, 775
SspMI CTAG 1 cut(s) 892
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 3 cut(s) 72, 384, 746
TaaI ACNGT 3 cut(s) 140, 528, 786
TaiI ACGT 4 cut(s) 300, 396, 496, 729
TaqI TCGA 5 cut(s) 14, 20, 26, 516, 537
TaqII GACCGA 1 cut(s) 176
TasI AATT 4 cut(s) 468, 476, 683, 790
TatI WGTACW 2 cut(s) 84, 786
TauI GCSGC 1 cut(s) 614
TspDTI ATGAA 2 cut(s) 35, 768
Van91I CCANNNNNTGG 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 653
XagI CCTNNNNNAGG 1 cut(s) 552
XapI RAATTY 3 cut(s) 468, 476, 683
XceI RCATGY 1 cut(s) 91
XmiI GTMKAC 2 cut(s) 390, 714
XspI CTAG 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.