MD00G1005200.v1.1

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005929
Physical Location & Seq
Reverse (-)
749410 .. 750615
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1005200.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGTCGGCCAGGGGATGGCGGATAATCCAATGAATATTCTGCTCATTCCTTCACCTCAACCAGTTGAGGTCTCCCTCGTTCAAGTTCCTGAGCACCATCCTTTTAGAGCTTCACCAAAAATCATGGCTTGGGCAGTTGATACTGTAAACGAAATTGTCAATAAAACTCTCAGAGCCGGAGTCATACTTGAAAAAATCATGGGTCCACTCTCCAGAGGCCATCTAGCGCTGAGGAACACAAACCTTAATGACAATTCATTTGTCACCTTTAACTACTTCAAAGAACCAGAGGACTTAAGGAAGTGCATTGAGGGAATGAAGACCATTATCGATGTTGTGAACTCCGATGCCTACTCAAAGTTCCGTTACAAATACATGCCGGTCGAAGCTCTCATTGACCTGATGTTGGCTTTACCGTTGAACAGAAGGCGAAAACATGCCAACGTTACATTCTGTTTGGAACAATTCTGCATAGACACTGTATTGACCATATGTCACTATCATGGAGGTTGTCGAGTTGGGAGGGTTGTTGATAAAGGATATAGGGTTCCTGGCATTGATTCTCTTAGGGTGGTTGATGGATCGACGTTTTATCGCACCCCGGGTACTAACCCTCAAGCTACTGTTATGATGCTCGGAAGGCACATGGGACAAAGGATTCTGCATGATGGATTACTCCGTGGATCGAAGAAGAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

234

Amino Acids

26.26

Weight (kDa)

9.44

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 69 - 213 2.8e-22 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 16
AciI CCGC 1 cut(s) 20
AclI AACGTT 1 cut(s) 444
AclWI GGATC 2 cut(s) 589, 691
AcoI YGGCCR 1 cut(s) 7
AfaI GTAC 1 cut(s) 607
AfeI AGCGCT 1 cut(s) 228
AfiI CCNNNNNNNGG 2 cut(s) 16, 406
AflII CTTAAG 1 cut(s) 295
AgsI TTSAA 4 cut(s) 83, 191, 280, 421
AhdI GACNNNNNGTC 1 cut(s) 492
AjnI CCWGG 2 cut(s) 9, 550
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AloI GAACNNNNNNTCC 2 cut(s) 531, 563
AluBI AGCT 3 cut(s) 110, 389, 620
AluI AGCT 3 cut(s) 110, 389, 620
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 76
AlwI GGATC 2 cut(s) 589, 691
Ama87I CYCGRG 1 cut(s) 601
Aor51HI AGCGCT 1 cut(s) 228
AoxI GGCC 2 cut(s) 7, 217
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 2 cut(s) 602, 603
AsuHPI GGTGA 3 cut(s) 45, 105, 256
AvaI CYCGRG 1 cut(s) 601
AvaII GGWCC 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 230
BccI CCATC 5 cut(s) 10, 105, 228, 572, 662
BciT130I CCWGG 2 cut(s) 11, 552
BcnI CCSGG 2 cut(s) 602, 603
BcoDI GTCTC 1 cut(s) 76
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 1 cut(s) 230
BfrI CTTAAG 1 cut(s) 295
Bme1390I CCNGG 4 cut(s) 11, 552, 602, 603
Bme18I GGWCC 1 cut(s) 203
BmeRI GACNNNNNGTC 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 204, 549
BmrFI CCNGG 4 cut(s) 11, 552, 602, 603
BmsI GCATC 2 cut(s) 337, 621
BpiI GAAGAC 1 cut(s) 326
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 2 cut(s) 90, 230
BpuEI CTTGAG 1 cut(s) 600
BpuMI CCSGG 2 cut(s) 602, 603
Bsa29I ATCGAT 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 4 cut(s) 10, 600, 601, 679
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 406
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 62
BseBI CCWGG 2 cut(s) 11, 552
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 4 cut(s) 10, 600, 601, 679
BseGI GGATG 2 cut(s) 21, 97
BseLI CCNNNNNNNGG 2 cut(s) 16, 406
BseMII CTCAG 3 cut(s) 81, 184, 221
BseNI ACTGG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 384
BshFI GGCC 2 cut(s) 9, 219
BshVI ATCGAT 1 cut(s) 330
BsiEI CGRYCG 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 3 cut(s) 177, 380, 602
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 2 cut(s) 16, 406
BsmAI GTCTC 1 cut(s) 76
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 2 cut(s) 9, 219
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 581, 683
BspACI CCGC 1 cut(s) 20
BspANI GGCC 2 cut(s) 9, 219
BspCNI CTCAG 3 cut(s) 82, 183, 222
BspDI ATCGAT 1 cut(s) 330
BspLI GGNNCC 2 cut(s) 204, 549
BspPI GGATC 2 cut(s) 589, 691
BspTI CTTAAG 1 cut(s) 295
BspTNI GGTCTC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 1 cut(s) 62
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 4 cut(s) 10, 600, 601, 679
BssMI GATC 2 cut(s) 581, 683
Bst2UI CCWGG 2 cut(s) 11, 552
Bst4CI ACNGT 4 cut(s) 145, 417, 481, 625
BstAFI CTTAAG 1 cut(s) 295
BstDEI CTNAG 4 cut(s) 90, 170, 230, 566
BstDSI CCRYGG 1 cut(s) 679
BstF5I GGATG 2 cut(s) 21, 97
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 2 cut(s) 584, 686
BstMAI GTCTC 1 cut(s) 76
BstMBI GATC 2 cut(s) 581, 683
BstMCI CGRYCG 1 cut(s) 384
BstMWI GCNNNNNNNGC 1 cut(s) 640
BstNI CCWGG 2 cut(s) 11, 552
BstNSI RCATGY 2 cut(s) 379, 440
BstSCI CCNGG 4 cut(s) 9, 550, 600, 601
BstV2I GAAGAC 1 cut(s) 326
Bsu15I ATCGAT 1 cut(s) 330
BsuRI GGCC 2 cut(s) 9, 219
BsuTUI ATCGAT 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 679
BtsCI GGATG 2 cut(s) 21, 97
BtsIMutI CAGTG 1 cut(s) 477
CfoI GCGC 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 203
Cfr9I CCCGGG 1 cut(s) 601
ClaI ATCGAT 1 cut(s) 330
Csp6I GTAC 1 cut(s) 606
CviAII CATG 7 cut(s) 124, 199, 376, 437, 503, 646, 665
CviJI RGCY 8 cut(s) 9, 110, 128, 176, 219, 389, 410, 620
CviKI_1 RGCY 8 cut(s) 9, 110, 128, 176, 219, 389, 410, 620
CviQI GTAC 1 cut(s) 606
DdeI CTNAG 4 cut(s) 90, 170, 230, 566
DpnI GATC 2 cut(s) 583, 685
DpnII GATC 2 cut(s) 581, 683
DriI GACNNNNNGTC 1 cut(s) 492
EaeI YGGCCR 1 cut(s) 7
Eam1105I GACNNNNNGTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 203
Eco47III AGCGCT 1 cut(s) 228
Eco88I CYCGRG 1 cut(s) 601
EcoRII CCWGG 2 cut(s) 9, 550
FaeI CATG 7 cut(s) 127, 202, 379, 440, 506, 649, 668
FaqI GGGAC 1 cut(s) 663
FatI CATG 7 cut(s) 123, 198, 375, 436, 502, 645, 664
FauNDI CATATG 1 cut(s) 491
FokI GGATG 2 cut(s) 28, 84
FspBI CTAG 1 cut(s) 224
GlaI GCGC 1 cut(s) 228
GsuI CTGGAG 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 2 cut(s) 9, 219
HapII CCGG 3 cut(s) 177, 380, 602
HhaI GCGC 1 cut(s) 229
Hin1II CATG 7 cut(s) 127, 202, 379, 440, 506, 649, 668
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HinfI GANTC 3 cut(s) 180, 560, 658
HpaII CCGG 3 cut(s) 177, 380, 602
HphI GGTGA 3 cut(s) 45, 105, 256
Hpy166II GTNNAC 3 cut(s) 148, 206, 340
Hpy188I TCNGA 3 cut(s) 173, 346, 638
Hpy188III TCNNGA 2 cut(s) 89, 213
Hpy8I GTNNAC 3 cut(s) 148, 206, 340
Hpy99I CGWCG 1 cut(s) 589
HpyAV CCTTC 3 cut(s) 60, 420, 633
HpyCH4III ACNGT 4 cut(s) 145, 417, 481, 625
HpyCH4IV ACGT 2 cut(s) 444, 587
HpyCH4V TGCA 3 cut(s) 306, 471, 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 640
HpyF3I CTNAG 4 cut(s) 90, 170, 230, 566
HpySE526I ACGT 2 cut(s) 444, 587
Hsp92II CATG 7 cut(s) 127, 202, 379, 440, 506, 649, 668
HspAI GCGC 1 cut(s) 227
Kzo9I GATC 2 cut(s) 581, 683
LweI GCATC 2 cut(s) 337, 621
MaeI CTAG 1 cut(s) 224
MaeII ACGT 2 cut(s) 444, 587
MaeIII GTNAC 4 cut(s) 262, 365, 445, 494
MalI GATC 2 cut(s) 583, 685
MboI GATC 2 cut(s) 581, 683
MboII GAAGA 2 cut(s) 331, 700
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 3 cut(s) 153, 253, 464
MlyI GAGTC 1 cut(s) 189
MseI TTAA 3 cut(s) 246, 270, 296
MslI CAYNNNNRTG 1 cut(s) 501
MspCI CTTAAG 1 cut(s) 295
MspI CCGG 3 cut(s) 177, 380, 602
MspR9I CCNGG 4 cut(s) 11, 552, 602, 603
MvaI CCWGG 2 cut(s) 11, 552
MwoI GCNNNNNNNGC 1 cut(s) 640
NciI CCSGG 2 cut(s) 602, 603
NdeI CATATG 1 cut(s) 491
NdeII GATC 2 cut(s) 581, 683
NlaIII CATG 7 cut(s) 127, 202, 379, 440, 506, 649, 668
NlaIV GGNNCC 2 cut(s) 204, 549
NmuCI GTSAC 2 cut(s) 262, 494
NspI RCATGY 2 cut(s) 379, 440
PfeI GAWTC 2 cut(s) 560, 658
PflMI CCANNNNNTGG 1 cut(s) 16
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Psp1406I AACGTT 1 cut(s) 444
Psp6I CCWGG 2 cut(s) 9, 550
PspGI CCWGG 2 cut(s) 9, 550
PspN4I GGNNCC 2 cut(s) 204, 549
PspPI GGNCC 1 cut(s) 203
RsaI GTAC 1 cut(s) 607
RsaNI GTAC 1 cut(s) 606
RseI CAYNNNNRTG 1 cut(s) 501
SaqAI TTAA 3 cut(s) 246, 270, 296
Sau3AI GATC 2 cut(s) 581, 683
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 4 cut(s) 11, 552, 602, 603
SduI GDGCHC 1 cut(s) 96
SfaNI GCATC 2 cut(s) 337, 621
SinI GGWCC 1 cut(s) 203
SmaI CCCGGG 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 2 cut(s) 295, 615
SmoI CTYRAG 2 cut(s) 295, 615
Sse9I AATT 3 cut(s) 153, 253, 464
SsiI CCGC 1 cut(s) 20
SspI AATATT 1 cut(s) 37
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 4 cut(s) 9, 550, 600, 601
TaaI ACNGT 4 cut(s) 145, 417, 481, 625
TaiI ACGT 2 cut(s) 447, 590
TaqI TCGA 5 cut(s) 330, 384, 514, 584, 686
TasI AATT 3 cut(s) 153, 253, 464
TfiI GAWTC 2 cut(s) 560, 658
Tru1I TTAA 3 cut(s) 246, 270, 296
Tru9I TTAA 3 cut(s) 246, 270, 296
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 2 cut(s) 262, 494
Tsp45I GTSAC 2 cut(s) 262, 494
TspDTI ATGAA 3 cut(s) 47, 246, 332
TspGWI ACGGA 2 cut(s) 353, 668
TspMI CCCGGG 1 cut(s) 601
TspRI CASTG 1 cut(s) 484
Van91I CCANNNNNTGG 1 cut(s) 16
Vha464I CTTAAG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 203
XceI RCATGY 2 cut(s) 379, 440
XmaI CCCGGG 1 cut(s) 601
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.