RLG00000017345

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005929
Physical Location & Seq
Forward (+)
15527074 .. 15528813
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017345

Sequence Viewer

Length: 732 bp
ATGCCGTCGTCGCTTCTTCGAGGCCGTCGTCTCGGAACCCACGCCGGCGTCGTCAAGAGCCAAGTTACCTTAGGTTTGTTTCCAACGCCACTGACTTCCCATCGGGGGAGTACTATAAGGACTACATTATCAGCGGAGGTGGCACTGCTGGGTGCTCTTTGGCTGCGACACTATCATCCAGCACTCATGGATGTCAATGCCTTTGACTCTCCCACACAAGCCTTCACATCTGAAGATGGTGTTCCAAATGTCCAAGGACGCATTCTTGGAGGCAGCAGGGCCATGAATGCTGGGGCTTACATTGAATCAGCCTCCACTGTTGTCCAATTTGCATCCCCAGCCCGTACTGTGGCCACTCTCATGGAGAAGATTCTCGGGCCAGTCTCTGCTGGTTCAACTGATGTCAAACCAGTGGTCAGTGGATCTGATGTTCTCAGGACCAAGTCTATGGATGATTTCAAGCTCCAGGGGTGGTTTGGGAATAAGGATTTTAGGTTTATAGGGCCTGCATTGCCAGTTGATCAGTCTGACTTTGACCAGATGGCAGATTTCTGCCGCCGCACTGTAAGCACCGTATGGCATTACCATGGGGGCTGCATTGCCGGGAAGGTGGTTGATGGTGATTTCCGCGTCATTGTACCACTCCGTACTTTTGGTATATCACCCAGGGACAAATCCTCTGGCTACCTTTCTGATACTTGGGAGATATGTTGGGCAGAAGATAATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

244

Amino Acids

26.35

Weight (kDa)

7.01

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 630
AciI CCGC 4 cut(s) 134, 556, 559, 628
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 351
AcuI CTGAAG 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 4 cut(s) 112, 346, 639, 649
AfiI CCNNNNNNNGG 2 cut(s) 105, 349
AgsI TTSAA 3 cut(s) 305, 396, 460
AjnI CCWGG 2 cut(s) 465, 665
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AloI GAACNNNNNNTCC 2 cut(s) 414, 446
AluBI AGCT 1 cut(s) 463
AluI AGCT 1 cut(s) 463
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 35, 388
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 5 cut(s) 22, 279, 351, 377, 503
ApeKI GCWGC 3 cut(s) 163, 273, 594
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AspS9I GGNCC 4 cut(s) 279, 377, 438, 503
AsuC2I CCSGG 1 cut(s) 604
AsuHPI GGTGA 2 cut(s) 632, 654
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 1 cut(s) 438
AxyI CCTNAGG 1 cut(s) 70
BalI TGGCCA 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 157
BbvI GCAGC 3 cut(s) 150, 285, 581
BccI CCATC 4 cut(s) 108, 230, 535, 611
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 2 cut(s) 467, 667
BclI TGATCA 1 cut(s) 520
BcnI CCSGG 1 cut(s) 604
BcoDI GTCTC 2 cut(s) 35, 388
BisI GCNGC 5 cut(s) 164, 274, 556, 559, 595
BlsI GCNGC 5 cut(s) 165, 275, 557, 560, 596
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 3 cut(s) 467, 604, 667
Bme18I GGWCC 1 cut(s) 438
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 4 cut(s) 279, 377, 438, 503
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 3 cut(s) 467, 604, 667
BmsI GCATC 1 cut(s) 341
BpmI CTGGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 5 cut(s) 253, 466, 586, 665, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 349
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 3 cut(s) 380, 410, 515
Bse21I CCTNAGG 1 cut(s) 70
Bse3DI GCAATG 2 cut(s) 509, 597
BseBI CCWGG 2 cut(s) 467, 667
BseDI CCNNGG 5 cut(s) 253, 466, 586, 665, 666
BseGI GGATG 4 cut(s) 175, 196, 332, 457
BseLI CCNNNNNNNGG 2 cut(s) 105, 349
BseMI GCAATG 2 cut(s) 509, 597
BseMII CTCAG 1 cut(s) 448
BseNI ACTGG 3 cut(s) 380, 410, 515
BseXI GCAGC 3 cut(s) 150, 285, 581
BseYI CCCAGC 3 cut(s) 148, 290, 337
Bsh1236I CGCG 1 cut(s) 630
BshFI GGCC 5 cut(s) 24, 281, 353, 379, 505
BsiHKAI GWGCWC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 2 cut(s) 45, 603
BslFI GGGAC 1 cut(s) 683
BslI CCNNNNNNNGG 2 cut(s) 105, 349
BsmAI GTCTC 2 cut(s) 35, 388
BsmBI CGTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 683
BsmI GAATGC 2 cut(s) 261, 292
BsnI GGCC 5 cut(s) 24, 281, 353, 379, 505
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 157
Bsp143I GATC 2 cut(s) 422, 520
Bsp19I CCATGG 1 cut(s) 586
BspACI CCGC 4 cut(s) 134, 556, 559, 628
BspANI GGCC 5 cut(s) 24, 281, 353, 379, 505
BspCNI CTCAG 1 cut(s) 447
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 1 cut(s) 37
BspPI GGATC 1 cut(s) 430
BsrDI GCAATG 2 cut(s) 509, 597
BsrFI RCCGGY 1 cut(s) 44
BsrI ACTGG 3 cut(s) 380, 410, 515
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 5 cut(s) 253, 466, 586, 665, 666
BssMI GATC 2 cut(s) 422, 520
BssNI GRCGYC 1 cut(s) 48
BssT1I CCWWGG 2 cut(s) 253, 586
Bst2UI CCWGG 2 cut(s) 467, 667
Bst4CI ACNGT 4 cut(s) 319, 349, 565, 574
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 46, 507
BstDEI CTNAG 2 cut(s) 70, 434
BstDSI CCRYGG 1 cut(s) 586
BstF5I GGATG 4 cut(s) 175, 196, 332, 457
BstFNI CGCG 1 cut(s) 630
BstKTI GATC 2 cut(s) 425, 523
BstMAI GTCTC 2 cut(s) 35, 388
BstMBI GATC 2 cut(s) 422, 520
BstMWI GCNNNNNNNGC 6 cut(s) 10, 140, 287, 338, 511, 567
BstNI CCWGG 2 cut(s) 467, 667
BstSCI CCNGG 3 cut(s) 465, 602, 665
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 3 cut(s) 150, 285, 581
BstX2I RGATCY 1 cut(s) 422
BstXI CCANNNNNNTGG 2 cut(s) 361, 448
BstYI RGATCY 1 cut(s) 422
Bsu36I CCTNAGG 1 cut(s) 70
BsuRI GGCC 5 cut(s) 24, 281, 353, 379, 505
BtgI CCRYGG 1 cut(s) 586
BtsCI GGATG 4 cut(s) 175, 196, 332, 457
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 6 cut(s) 89, 143, 315, 417, 424, 561
Cac8I GCNNGC 2 cut(s) 46, 507
CaiI CAGNNNCTG 1 cut(s) 386
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 4 cut(s) 279, 377, 438, 503
CseI GACGC 3 cut(s) 37, 267, 619
Csp6I GTAC 4 cut(s) 111, 345, 638, 648
CviAII CATG 4 cut(s) 187, 283, 361, 587
CviQI GTAC 4 cut(s) 111, 345, 638, 648
DdeI CTNAG 2 cut(s) 70, 434
DpnI GATC 2 cut(s) 424, 522
DpnII GATC 2 cut(s) 422, 520
EaeI YGGCCR 1 cut(s) 351
Eco130I CCWWGG 2 cut(s) 253, 586
Eco47I GGWCC 1 cut(s) 438
Eco57I CTGAAG 1 cut(s) 252
Eco81I CCTNAGG 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 503
EcoRII CCWGG 2 cut(s) 465, 665
EcoT14I CCWWGG 2 cut(s) 253, 586
ErhI CCWWGG 2 cut(s) 253, 586
Esp3I CGTCTC 1 cut(s) 35
FaeI CATG 4 cut(s) 190, 286, 364, 590
FaqI GGGAC 1 cut(s) 683
FatI CATG 4 cut(s) 186, 282, 360, 586
FbaI TGATCA 1 cut(s) 520
Fnu4HI GCNGC 5 cut(s) 164, 274, 556, 559, 595
FokI GGATG 4 cut(s) 162, 203, 319, 464
Fsp4HI GCNGC 5 cut(s) 164, 274, 556, 559, 595
GluI GCNGC 5 cut(s) 164, 274, 556, 559, 595
GsaI CCCAGC 3 cut(s) 152, 294, 341
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 5 cut(s) 24, 281, 353, 379, 505
HapII CCGG 2 cut(s) 45, 603
HgaI GACGC 3 cut(s) 37, 267, 619
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 4 cut(s) 190, 286, 364, 590
HinfI GANTC 3 cut(s) 206, 305, 370
HpaII CCGG 2 cut(s) 45, 603
HphI GGTGA 2 cut(s) 632, 654
Hpy188I TCNGA 5 cut(s) 35, 232, 427, 529, 694
Hpy188III TCNNGA 2 cut(s) 55, 436
Hpy99I CGWCG 4 cut(s) 10, 13, 30, 53
HpyAV CCTTC 2 cut(s) 232, 601
HpyCH4III ACNGT 4 cut(s) 319, 349, 565, 574
HpyCH4V TGCA 3 cut(s) 332, 509, 597
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 140, 287, 338, 511, 567
HpyF3I CTNAG 2 cut(s) 70, 434
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 4 cut(s) 190, 286, 364, 590
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 2 cut(s) 422, 520
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 3 cut(s) 150, 285, 581
LweI GCATC 1 cut(s) 341
MaeIII GTNAC 1 cut(s) 64
MalI GATC 2 cut(s) 424, 522
MboI GATC 2 cut(s) 422, 520
MboII GAAGA 4 cut(s) 8, 245, 379, 731
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 157
MlsI TGGCCA 1 cut(s) 353
MluCI AATT 1 cut(s) 326
MluNI TGGCCA 1 cut(s) 353
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 107
MnlI CCTC 5 cut(s) 14, 130, 263, 322, 688
Mox20I TGGCCA 1 cut(s) 353
MreI CGCCGGCG 1 cut(s) 44
MroNI GCCGGC 1 cut(s) 44
MscI TGGCCA 1 cut(s) 353
MslI CAYNNNNRTG 2 cut(s) 359, 585
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 2 cut(s) 45, 603
MspR9I CCNGG 3 cut(s) 467, 604, 667
Mva1269I GAATGC 2 cut(s) 261, 292
MvaI CCWGG 2 cut(s) 467, 667
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 6 cut(s) 10, 140, 287, 338, 511, 567
NaeI GCCGGC 1 cut(s) 46
NciI CCSGG 1 cut(s) 604
NcoI CCATGG 1 cut(s) 586
NdeII GATC 2 cut(s) 422, 520
NgoMIV GCCGGC 1 cut(s) 44
NlaIII CATG 4 cut(s) 190, 286, 364, 590
NlaIV GGNNCC 1 cut(s) 37
PasI CCCWGGG 1 cut(s) 666
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 48
PctI GAATGC 2 cut(s) 261, 292
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 2 cut(s) 305, 370
PflFI GACNNNGTC 1 cut(s) 442
PkrI GCNGC 5 cut(s) 165, 275, 557, 560, 596
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 2 cut(s) 465, 665
PspFI CCCAGC 3 cut(s) 148, 290, 337
PspGI CCWGG 2 cut(s) 465, 665
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 4 cut(s) 279, 377, 438, 503
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 422
PsyI GACNNNGTC 1 cut(s) 442
RsaI GTAC 4 cut(s) 112, 346, 639, 649
RsaNI GTAC 4 cut(s) 111, 345, 638, 648
RseI CAYNNNNRTG 2 cut(s) 359, 585
SatI GCNGC 5 cut(s) 164, 274, 556, 559, 595
Sau3AI GATC 2 cut(s) 422, 520
Sau96I GGNCC 4 cut(s) 279, 377, 438, 503
ScaI AGTACT 1 cut(s) 112
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 3 cut(s) 467, 604, 667
SduI GDGCHC 1 cut(s) 157
SetI ASST 7 cut(s) 71, 76, 141, 465, 497, 612, 690
SfaNI GCATC 1 cut(s) 341
SgrAI CRCCGGYG 1 cut(s) 44
SinI GGWCC 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 359, 585
Sse9I AATT 1 cut(s) 326
SsiI CCGC 4 cut(s) 134, 556, 559, 628
StyD4I CCNGG 3 cut(s) 465, 602, 665
StyI CCWWGG 2 cut(s) 253, 586
TaaI ACNGT 4 cut(s) 319, 349, 565, 574
TaqI TCGA 1 cut(s) 19
TasI AATT 1 cut(s) 326
TatI WGTACW 1 cut(s) 110
TauI GCSGC 2 cut(s) 558, 561
TfiI GAWTC 2 cut(s) 305, 370
TscAI CASTG 6 cut(s) 96, 150, 322, 417, 424, 568
TseI GCWGC 3 cut(s) 163, 273, 594
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 6 cut(s) 96, 150, 322, 417, 424, 568
Tth111I GACNNNGTC 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 438
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.