MD03G1091500.v1.1

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005929
Physical Location & Seq
Forward (+)
7594822 .. 7595187
366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1091500.v1.1.491

Sequence Viewer

Length: 366 bp
ATGGGTGATTTATTAAAAACAAACTCATTGAAACCGTATAAGGCTCAAGATTTGCTAGTTCTAGAAGGTTTCAACCTTTTTGGACCGCCCTTACCAGTGAACCAGTCAGATGATGCATCATTTGAAACATTTTGTCGAGATACAGTAGCCACATTTTGGCACTTCCATGGTGGATGCCTGGTCGGAAAGGTAGTTGATGAAGATTTGCGGGTCATGGGGATCAAGGCATTACGTGTTGTTGATGGATCTGTATTCAATTTATCACCAAGGACCAATCCTCAAGCCACTCTTATGATGTTGGGCAGGTATGCACGTTACATGTGCATTATGATGTTAGGCAGGTATGCATGTTACATGTGCATTTAA

Protein Analysis

122

Amino Acids

13.61

Weight (kDa)

7.62

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 34 - 101 7.1e-18 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 294, 330
AccB7I CCANNNNNTGG 1 cut(s) 156
AciI CCGC 2 cut(s) 86, 208
AclWI GGATC 2 cut(s) 227, 253
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 3 cut(s) 232, 318, 354
AgsI TTSAA 4 cut(s) 31, 73, 125, 256
AjnI CCWGG 1 cut(s) 177
AlwI GGATC 2 cut(s) 227, 253
AspS9I GGNCC 2 cut(s) 83, 270
AsuHPI GGTGA 2 cut(s) 17, 255
AvaII GGWCC 2 cut(s) 83, 270
BccI CCATC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 179
BfaI CTAG 2 cut(s) 56, 62
BfuAI ACCTGC 2 cut(s) 294, 330
Bme1390I CCNGG 1 cut(s) 179
Bme18I GGWCC 2 cut(s) 83, 270
BmgT120I GGNCC 2 cut(s) 83, 270
BmrFI CCNGG 1 cut(s) 179
BmsI GCATC 3 cut(s) 103, 125, 164
BpuEI CTTGAG 2 cut(s) 30, 264
BsaAI YACGTR 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 166, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 95, 103
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 166, 266
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 95, 103
BslI CCNNNNNNNGG 1 cut(s) 156
Bsp143I GATC 2 cut(s) 219, 245
Bsp19I CCATGG 1 cut(s) 166
BspACI CCGC 2 cut(s) 86, 208
BspMI ACCTGC 2 cut(s) 294, 330
BspPI GGATC 2 cut(s) 227, 253
BsrI ACTGG 2 cut(s) 95, 103
BssECI CCNNGG 2 cut(s) 166, 266
BssMI GATC 2 cut(s) 219, 245
BssT1I CCWWGG 2 cut(s) 166, 266
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 2 cut(s) 36, 145
BstBAI YACGTR 1 cut(s) 233
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 2 cut(s) 222, 248
BstMBI GATC 2 cut(s) 219, 245
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 3 cut(s) 322, 351, 358
BstSCI CCNGG 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 2 cut(s) 294, 330
Cfr13I GGNCC 2 cut(s) 83, 270
CviAII CATG 5 cut(s) 167, 214, 319, 348, 355
CviJI RGCY 3 cut(s) 44, 149, 284
CviKI_1 RGCY 3 cut(s) 44, 149, 284
DpnI GATC 2 cut(s) 221, 247
DpnII GATC 2 cut(s) 219, 245
Eco130I CCWWGG 2 cut(s) 166, 266
Eco47I GGWCC 2 cut(s) 83, 270
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 2 cut(s) 166, 266
EcoT22I ATGCAT 2 cut(s) 118, 349
ErhI CCWWGG 2 cut(s) 166, 266
FaeI CATG 5 cut(s) 170, 217, 322, 351, 358
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FatI CATG 5 cut(s) 166, 213, 318, 347, 354
FauI CCCGC 1 cut(s) 201
FokI GGATG 1 cut(s) 186
FspBI CTAG 2 cut(s) 56, 62
Hin1II CATG 5 cut(s) 170, 217, 322, 351, 358
HphI GGTGA 2 cut(s) 17, 255
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 109, 185
Hpy188III TCNNGA 3 cut(s) 47, 62, 137
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 2 cut(s) 36, 145
HpyCH4IV ACGT 2 cut(s) 232, 313
HpyCH4V TGCA 5 cut(s) 116, 311, 324, 347, 360
HpySE526I ACGT 2 cut(s) 232, 313
Hsp92II CATG 5 cut(s) 170, 217, 322, 351, 358
Kzo9I GATC 2 cut(s) 219, 245
LpnPI CCDG 6 cut(s) 108, 116, 164, 191, 289, 325
LweI GCATC 3 cut(s) 103, 125, 164
MaeI CTAG 2 cut(s) 56, 62
MaeII ACGT 2 cut(s) 232, 313
MaeIII GTNAC 2 cut(s) 314, 350
MalI GATC 2 cut(s) 221, 247
MboI GATC 2 cut(s) 219, 245
MboII GAAGA 1 cut(s) 212
MflI RGATCY 1 cut(s) 245
MluCI AATT 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 1 cut(s) 288
Mph1103I ATGCAT 2 cut(s) 118, 349
MseI TTAA 2 cut(s) 14, 364
MslI CAYNNNNRTG 3 cut(s) 165, 290, 329
MspR9I CCNGG 1 cut(s) 179
MvaI CCWGG 1 cut(s) 179
NcoI CCATGG 1 cut(s) 166
NdeII GATC 2 cut(s) 219, 245
NlaIII CATG 5 cut(s) 170, 217, 322, 351, 358
NsiI ATGCAT 2 cut(s) 118, 349
NspI RCATGY 3 cut(s) 322, 351, 358
PciI ACATGT 2 cut(s) 318, 354
PflMI CCANNNNNTGG 1 cut(s) 156
Ppu21I YACGTR 1 cut(s) 233
PscI ACATGT 2 cut(s) 318, 354
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspPI GGNCC 2 cut(s) 83, 270
PsuI RGATCY 1 cut(s) 245
RseI CAYNNNNRTG 3 cut(s) 165, 290, 329
SaqAI TTAA 2 cut(s) 14, 364
Sau3AI GATC 2 cut(s) 219, 245
Sau96I GGNCC 2 cut(s) 83, 270
ScrFI CCNGG 1 cut(s) 179
SetI ASST 7 cut(s) 70, 78, 192, 235, 308, 316, 344
SfaNI GCATC 3 cut(s) 103, 125, 164
SinI GGWCC 2 cut(s) 83, 270
SmiMI CAYNNNNRTG 3 cut(s) 165, 290, 329
SmlI CTYRAG 2 cut(s) 45, 279
SmoI CTYRAG 2 cut(s) 45, 279
Sse9I AATT 1 cut(s) 256
SsiI CCGC 2 cut(s) 86, 208
SspMI CTAG 2 cut(s) 56, 62
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 2 cut(s) 166, 266
TaaI ACNGT 2 cut(s) 36, 145
TaiI ACGT 2 cut(s) 235, 316
TaqI TCGA 1 cut(s) 136
TasI AATT 1 cut(s) 256
Tru1I TTAA 2 cut(s) 14, 364
Tru9I TTAA 2 cut(s) 14, 364
TscAI CASTG 1 cut(s) 102
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 1 cut(s) 102
Van91I CCANNNNNTGG 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 83, 270
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 3 cut(s) 322, 351, 358
XspI CTAG 2 cut(s) 56, 62
Zsp2I ATGCAT 2 cut(s) 118, 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.