Rroxscaffold_2G00146080

(R)-mandelonitrile lyase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005929
Physical Location & Seq
Reverse (-)
83924910 .. 83933044
8135 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146080.1

Sequence Viewer

Length: 480 bp
ATGCCGTCAACGCTTCTTCAAGGCCGTCGTCTCGGAACCACGTCGGTGTTGTCAAGCCCGTACTGTGGCCACTCTCATGGAGAAGGTTCTCGAGCAATCTCTGCTGGTTCAACTGATGTCAAGGTCAATCCTATCATTCAATTTAATTACTTCAACAGCCTAGTGGTCAGTGGATGTGATGTTCTCAGGGCCAAGTCTATGCATGATTTCAAGTTCCAGGGATGGTTTGGGAATAAGGATTTTAGGTTTATAGGGCCTGCTTTGCCGGTTGATCCGTCTGACTTTGACCAGATGGCAAACTTCTGCCGCTGCACTGTAAGCACCATATGGCATTACCATGGGGGCGGCATAGCCGTGAAGGTGGTTGCTGGTGATTTCCTCGTCATTGGTATAGATGCACTCCGTACTTTTGGTATATCATCGGGGACACCAGGCTACCCTTCTGATGCTTGGAAGGTCAGTGTCCTCAAATCATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko01110 map00460 map01110
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

159

Amino Acids

17.15

Weight (kDa)

8.65

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 307, 345
AclWI GGATC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 67
AfaI GTAC 2 cut(s) 62, 406
AfiI CCNNNNNNNGG 1 cut(s) 65
AgsI TTSAA 5 cut(s) 20, 111, 140, 154, 211
AjiI CACGTC 1 cut(s) 42
AjnI CCWGG 2 cut(s) 216, 430
AleI CACNNNNGTG 1 cut(s) 44
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 1 cut(s) 266
Ama87I CYCGRG 1 cut(s) 90
AoxI GGCC 4 cut(s) 22, 67, 189, 254
ApeKI GCWGC 1 cut(s) 309
AspS9I GGNCC 2 cut(s) 189, 254
AsuHPI GGTGA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 90
BalI TGGCCA 1 cut(s) 69
BbvI GCAGC 1 cut(s) 296
BccI CCATC 2 cut(s) 216, 286
BceAI ACGGC 2 cut(s) 9, 338
BciT130I CCWGG 2 cut(s) 218, 432
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 1 cut(s) 161
BisI GCNGC 3 cut(s) 307, 310, 346
BlsI GCNGC 3 cut(s) 308, 311, 347
Bme1390I CCNGG 2 cut(s) 218, 432
BmeT110I CYCGRG 1 cut(s) 90
BmgBI CACGTC 1 cut(s) 42
BmgT120I GGNCC 2 cut(s) 189, 254
BmiI GGNNCC 1 cut(s) 37
BmrFI CCNGG 2 cut(s) 218, 432
BmsI GCATC 2 cut(s) 385, 436
BsaJI CCNNGG 2 cut(s) 217, 337
Bsc4I CCNNNNNNNGG 1 cut(s) 65
Bse118I RCCGGY 1 cut(s) 265
BseBI CCWGG 2 cut(s) 218, 432
BseDI CCNNGG 2 cut(s) 217, 337
BseGI GGATG 2 cut(s) 179, 227
BseLI CCNNNNNNNGG 1 cut(s) 65
BseMII CTCAG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 295
BshFI GGCC 4 cut(s) 24, 69, 191, 256
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 1 cut(s) 266
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 35
BsmBI CGTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 4 cut(s) 24, 69, 191, 256
BsoBI CYCGRG 1 cut(s) 90
Bsp143I GATC 1 cut(s) 271
Bsp19I CCATGG 1 cut(s) 337
BspACI CCGC 2 cut(s) 307, 345
BspANI GGCC 4 cut(s) 24, 69, 191, 256
BspCNI CTCAG 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 37
BspPI GGATC 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 265
BssECI CCNNGG 2 cut(s) 217, 337
BssMI GATC 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 2 cut(s) 218, 432
Bst4CI ACNGT 2 cut(s) 65, 316
BstAPI GCANNNNNTGC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 1 cut(s) 185
BstDSI CCRYGG 1 cut(s) 337
BstF5I GGATG 2 cut(s) 179, 227
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 4 cut(s) 10, 101, 262, 318
BstNI CCWGG 2 cut(s) 218, 432
BstSCI CCNGG 2 cut(s) 216, 430
BstV1I GCAGC 1 cut(s) 296
BstXI CCANNNNNNTGG 1 cut(s) 77
BsuRI GGCC 4 cut(s) 24, 69, 191, 256
BtgI CCRYGG 1 cut(s) 337
BtrI CACGTC 1 cut(s) 42
BtsCI GGATG 2 cut(s) 179, 227
BtsIMutI CAGTG 3 cut(s) 175, 312, 466
Cac8I GCNNGC 1 cut(s) 258
Cfr10I RCCGGY 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 189, 254
Csp6I GTAC 2 cut(s) 61, 405
CviAII CATG 4 cut(s) 77, 203, 338, 474
CviJI RGCY 8 cut(s) 24, 57, 69, 159, 191, 256, 353, 435
CviKI_1 RGCY 8 cut(s) 24, 57, 69, 159, 191, 256, 353, 435
CviQI GTAC 2 cut(s) 61, 405
DdeI CTNAG 1 cut(s) 185
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 337
Eco88I CYCGRG 1 cut(s) 90
EcoO109I RGGNCCY 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 216, 430
EcoT14I CCWWGG 1 cut(s) 337
EcoT22I ATGCAT 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 35
FaeI CATG 4 cut(s) 80, 206, 341, 477
FaqI GGGAC 1 cut(s) 439
FatI CATG 4 cut(s) 76, 202, 337, 473
FauNDI CATATG 1 cut(s) 326
Fnu4HI GCNGC 3 cut(s) 307, 310, 346
FokI GGATG 2 cut(s) 186, 234
Fsp4HI GCNGC 3 cut(s) 307, 310, 346
FspBI CTAG 1 cut(s) 161
GluI GCNGC 3 cut(s) 307, 310, 346
HaeIII GGCC 4 cut(s) 24, 69, 191, 256
HapII CCGG 1 cut(s) 266
Hin1II CATG 4 cut(s) 80, 206, 341, 477
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 3 cut(s) 35, 280, 445
Hpy188III TCNNGA 1 cut(s) 90
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 2 cut(s) 30, 46
HpyAV CCTTC 4 cut(s) 77, 352, 448, 450
HpyCH4III ACNGT 2 cut(s) 65, 316
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 3 cut(s) 202, 312, 398
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 101, 262, 318
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 4 cut(s) 80, 206, 341, 477
Kzo9I GATC 1 cut(s) 271
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 385, 436
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 41
MalI GATC 1 cut(s) 273
MboI GATC 1 cut(s) 271
MboII GAAGA 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 69
MluCI AATT 2 cut(s) 140, 145
MluNI TGGCCA 1 cut(s) 69
MnlI CCTC 2 cut(s) 389, 476
Mox20I TGGCCA 1 cut(s) 69
Mph1103I ATGCAT 1 cut(s) 204
MscI TGGCCA 1 cut(s) 69
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 4 cut(s) 44, 75, 336, 353
Msp20I TGGCCA 1 cut(s) 69
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 218, 432
MvaI CCWGG 2 cut(s) 218, 432
MwoI GCNNNNNNNGC 4 cut(s) 10, 101, 262, 318
NcoI CCATGG 1 cut(s) 337
NdeI CATATG 1 cut(s) 326
NdeII GATC 1 cut(s) 271
NlaIII CATG 4 cut(s) 80, 206, 341, 477
NlaIV GGNNCC 1 cut(s) 37
NsiI ATGCAT 1 cut(s) 204
OliI CACNNNNGTG 1 cut(s) 44
PaeR7I CTCGAG 1 cut(s) 90
PkrI GCNGC 3 cut(s) 308, 311, 347
Psp6I CCWGG 2 cut(s) 216, 430
PspGI CCWGG 2 cut(s) 216, 430
PspN4I GGNNCC 1 cut(s) 37
PspPI GGNCC 2 cut(s) 189, 254
RsaI GTAC 2 cut(s) 62, 406
RsaNI GTAC 2 cut(s) 61, 405
RseI CAYNNNNRTG 4 cut(s) 44, 75, 336, 353
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 307, 310, 346
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 2 cut(s) 189, 254
ScrFI CCNGG 2 cut(s) 218, 432
SetI ASST 6 cut(s) 44, 88, 126, 248, 363, 459
SfaNI GCATC 2 cut(s) 385, 436
Sfr274I CTCGAG 1 cut(s) 90
SlaI CTCGAG 1 cut(s) 90
SmiMI CAYNNNNRTG 4 cut(s) 44, 75, 336, 353
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 2 cut(s) 140, 145
SsiI CCGC 2 cut(s) 307, 345
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 2 cut(s) 216, 430
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 2 cut(s) 65, 316
TaiI ACGT 1 cut(s) 44
TaqI TCGA 1 cut(s) 91
TasI AATT 2 cut(s) 140, 145
TauI GCSGC 2 cut(s) 309, 348
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 3 cut(s) 175, 319, 466
TseI GCWGC 1 cut(s) 309
TspGWI ACGGA 2 cut(s) 264, 392
TspRI CASTG 3 cut(s) 175, 319, 466
XcmI CCANNNNNNNNNTGG 1 cut(s) 224
XhoI CTCGAG 1 cut(s) 90
XspI CTAG 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.