Rh4CG129100

(R)-mandelonitrile lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005929
Physical Location & Seq
Reverse (-)
25319367 .. 25321416
2050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG129100.1

Sequence Viewer

Length: 354 bp
ATGAAGAAGATTGGGGAGTTATTAAATACGAAGTCACTGAAACCATTTAAGGTTCAAGCTTCGTCGAGCACAAAAGCTTACAATTTTTATGGTCCAACTTTACCTATGAACCAGACAGATGATGCATCTTTTGGAGAATTTTGTCGTGATACAGTATCAACAATTTGGCATTTCCACGGCGGATGCCTTGTCAGAAAGGTGGTCGATAGCAGCTTGCGGGTCATGGGAATCAATGCTTTGCGTGTTGTTGACGCATCCACATTCAATTTCACACCGGGTACAAATCCTCAGGCCACACTTATGATGTTGGGCAGGTACATTGGCATTAAGATGCTGCAACAAAGATCTTCTTAA

Protein Analysis

117

Amino Acids

13.0

Weight (kDa)

9.8

Isoelectric Point (pI)

23.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 36 - 104 1.3e-15 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 303
AciI CCGC 2 cut(s) 180, 217
AcsI RAATTY 1 cut(s) 137
AfaI GTAC 2 cut(s) 280, 317
AgsI TTSAA 2 cut(s) 56, 265
AluBI AGCT 3 cut(s) 59, 77, 213
AluI AGCT 3 cut(s) 59, 77, 213
Alw21I GWGCWC 1 cut(s) 71
AoxI GGCC 1 cut(s) 291
ApeKI GCWGC 2 cut(s) 210, 334
ApoI RAATTY 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 92
AsuC2I CCSGG 1 cut(s) 276
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 2 cut(s) 222, 321
BceAI ACGGC 1 cut(s) 193
BcnI CCSGG 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 303
BglII AGATCT 1 cut(s) 344
BisI GCNGC 2 cut(s) 211, 335
BlsI GCNGC 2 cut(s) 212, 336
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BmrFI CCNGG 1 cut(s) 276
BmsI GCATC 5 cut(s) 112, 134, 173, 263, 321
BpuMI CCSGG 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 288
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 2 cut(s) 188, 254
BseMII CTCAG 1 cut(s) 302
BseXI GCAGC 2 cut(s) 222, 321
BshFI GGCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 275
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 71
Bsp143I GATC 1 cut(s) 344
BspACI CCGC 2 cut(s) 180, 217
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 301
BspMI ACCTGC 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 1 cut(s) 344
Bst4CI ACNGT 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 2 cut(s) 188, 254
BstKTI GATC 1 cut(s) 347
BstMBI GATC 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 222, 321
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
Bsu36I CCTNAGG 1 cut(s) 288
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 2 cut(s) 188, 254
BtsIMutI CAGTG 1 cut(s) 35
BveI ACCTGC 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 215
Cfr13I GGNCC 1 cut(s) 92
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 279, 316
CviAII CATG 1 cut(s) 223
CviJI RGCY 4 cut(s) 59, 77, 213, 293
CviKI_1 RGCY 4 cut(s) 59, 77, 213, 293
CviQI GTAC 2 cut(s) 279, 316
DdeI CTNAG 1 cut(s) 288
DpnI GATC 1 cut(s) 346
DpnII GATC 1 cut(s) 344
EciI GGCGGA 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 127
FaeI CATG 1 cut(s) 226
FaiI YATR 4 cut(s) 90, 107, 224, 302
FalI AAGNNNNNCTT 1 cut(s) 334
FatI CATG 1 cut(s) 222
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 2 cut(s) 211, 335
FokI GGATG 2 cut(s) 195, 241
Fsp4HI GCNGC 2 cut(s) 211, 335
GluI GCNGC 2 cut(s) 211, 335
HaeIII GGCC 1 cut(s) 293
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 260
Hin1II CATG 1 cut(s) 226
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 2 cut(s) 57, 75
HinfI GANTC 1 cut(s) 228
HpaII CCGG 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 1 cut(s) 250
Hpy99I CGWCG 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 125, 337
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 1 cut(s) 226
Kzo9I GATC 1 cut(s) 344
LpnPI CCDG 4 cut(s) 125, 275, 288, 298
Lsp1109I GCAGC 2 cut(s) 222, 321
LweI GCATC 5 cut(s) 112, 134, 173, 263, 321
MaeIII GTNAC 1 cut(s) 33
MalI GATC 1 cut(s) 346
MboI GATC 1 cut(s) 344
MboII GAAGA 3 cut(s) 16, 19, 339
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 71
MluCI AATT 4 cut(s) 82, 137, 162, 265
MmeI TCCRAC 1 cut(s) 119
MnlI CCTC 1 cut(s) 297
Mph1103I ATGCAT 1 cut(s) 127
MseI TTAA 4 cut(s) 23, 48, 327, 352
MslI CAYNNNNRTG 2 cut(s) 299, 329
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 276
NciI CCSGG 1 cut(s) 276
NdeII GATC 1 cut(s) 344
NlaIII CATG 1 cut(s) 226
NmuCI GTSAC 1 cut(s) 33
NsiI ATGCAT 1 cut(s) 127
PfeI GAWTC 1 cut(s) 228
PkrI GCNGC 2 cut(s) 212, 336
PspPI GGNCC 1 cut(s) 92
PsuI RGATCY 1 cut(s) 344
RsaI GTAC 2 cut(s) 280, 317
RsaNI GTAC 2 cut(s) 279, 316
RseI CAYNNNNRTG 2 cut(s) 299, 329
SaqAI TTAA 4 cut(s) 23, 48, 327, 352
SatI GCNGC 2 cut(s) 211, 335
Sau3AI GATC 1 cut(s) 344
Sau96I GGNCC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 1 cut(s) 71
SetI ASST 7 cut(s) 54, 61, 79, 106, 201, 215, 317
SfaNI GCATC 5 cut(s) 112, 134, 173, 263, 321
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 299, 329
Sse9I AATT 4 cut(s) 82, 137, 162, 265
SsiI CCGC 2 cut(s) 180, 217
StyD4I CCNGG 1 cut(s) 274
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 2 cut(s) 65, 204
TasI AATT 4 cut(s) 82, 137, 162, 265
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 4 cut(s) 23, 48, 327, 352
Tru9I TTAA 4 cut(s) 23, 48, 327, 352
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 1 cut(s) 33
TseI GCWGC 2 cut(s) 210, 334
Tsp45I GTSAC 1 cut(s) 33
TspDTI ATGAA 2 cut(s) 17, 122
TspRI CASTG 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.